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Variations in sap flow of Zenia insignis under different rock bareness rate in North Guangdong,China 被引量:3
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作者 LI Hui-xia ZHOU Hong-yi +2 位作者 WEI Xing-hu LU Nan LIANG Zhao-xiong 《Journal of Mountain Science》 SCIE CSCD 2019年第10期2320-2334,共15页
Understanding the impact of rock bareness on the transpiration in karst plants is essential to karst rocky desertification control and sustainable management of plantation in karst area.This study focused on the varia... Understanding the impact of rock bareness on the transpiration in karst plants is essential to karst rocky desertification control and sustainable management of plantation in karst area.This study focused on the variations in sap flow of Zenia insignis caused by different rock bareness rate,and the impact of climate factors,soil water content(SWC)and leaf area index(LAI) on transpiration in karst plants,by continuously measuring sap flux densities (Fcd)of 12 sample trees using thermal dissipation probes and monitoring micrometeorology and SwC on a typical karst hill in north Guangdong of China during the year of 2016.Results show that:(1)the maximum hourly sap flux density occurred at11:00-14:00 and the peak daily sap flux density occurred in September.(2)Sap flow density of Zenia insignis increased with rock bareness rate at all hourly,daily and monthly scales,with the sequence of extremely severe>severe>moderate>mild rock bareness.(3)The transpiration of Zenia insi.gnis is controlled by different factors at different temporalscales.At hourly scale,transpiration was highly(n=144,R^2>0.72)correlated to Solar radiation(Rs),Air temperature(Ta),relative humidity(RH),and water vapor pressure deficit(VPD).At daily scale,transpiration was greatly(n=366,R2>0.31)affected by Solar radiation(Rs),Air temperature(Ta),and water vapor pressure deficit (VPD).While at monthly scale,transpiration was mainly(n=12,R^2=0.85)controlled by LAI.Our study proved that Zenia insi.gnis has a good physiecological adaption to fragile karst environment,and Zenia insignis plantation has long-term sustainability even in extremely rocky landscapes.The results may provide scientific basis for plantation management and ecological restoration in karst area. 展开更多
关键词 Sap flow Thermal DISSIPATION probes rock bareness KARST ECOSYSTEMS Zenia insignis
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“Baihui”(DU20)-penetrating “Qubin”(GB7) acupuncture on blood–brain barrier integrity in rat intracerebral hemorrhage models via the RhoA/ROCK Ⅱ/MLC 2 signaling pathway 被引量:1
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作者 Ce Zhang Jia Zheng +10 位作者 Xueping Yu Binglin Kuang Xiaohong Dai Lei Zheng Weiwei Yu Wei Teng Hongtao Cao Mingyue Li Jiayong Yao Xiaoying Liu Wei Zou 《Animal Models and Experimental Medicine》 CAS CSCD 2024年第5期740-757,共18页
Background: Blocking the Rho A/ROCK Ⅱ/MLC 2(Ras homolog gene family member A/Rho kinase Ⅱ/myosin light chain 2) signaling pathway can initiate neuroprotective mechanisms against neurological diseases such as stroke,... Background: Blocking the Rho A/ROCK Ⅱ/MLC 2(Ras homolog gene family member A/Rho kinase Ⅱ/myosin light chain 2) signaling pathway can initiate neuroprotective mechanisms against neurological diseases such as stroke, cerebral ischemia, and subarachnoid hemorrhage. Nevertheless, it is not clear whether and how disrupting the Rho A/ROCK Ⅱ/MLC 2 signaling pathway changes the pathogenic processes of the blood–brain barrier(BBB) after intracerebral hemorrhage(ICH). The present investigation included the injection of rat caudal vein blood into the basal ganglia area to replicate the pathophysiological conditions caused by ICH. Methods: Scalp acupuncture(SA) therapy was performed on rats with ICH at the acupuncture point “Baihui”-penetrating “Qubin,” and the ROCK selective inhibitor fasudil was used as a positive control to evaluate the inhibitory effect of acupuncture on the Rho A/ROCK Ⅱ/MLC 2 signaling pathway. Post-assessments included neurological deficits, brain edema, Evans blue extravasation, Western blot, quantitative polymerase chain reaction, and transmission electron microscope imaging. Results: We found that ROCK Ⅱ acts as a promoter of the Rho A/ROCK Ⅱ/MLC 2 signaling pathway, and its expression increased at 6 h after ICH, peaked at 3 days, and then decreased at 7 days after ICH, but was still higher than the preintervention level. According to some experimental results, although 3 days is the peak, 7 days is the best time point for acupuncture treatment. Starting from 6 h after ICH, the neurovascular structure and endothelial cell morphology around the hematoma began to change. Based on the changes in the promoter ROCK Ⅱ, a 7-day time point was selected as the breakthrough point for treating ICH model rats in the main experiment. The results of this experiment showed that both SA at “Baihui”-penetrating “Qubin” and treatment with fasudil could improve the expression of endothelial-related proteins by inhibiting the Rho A/ROCK Ⅱ/MLC 2 signaling pathway and reduce neurological dysfunction, brain edema, and BBB permeability in rats. Conclusion: This study found that these experimental data indicated that SA at “Baihui”-penetrating “Qubin” could preserve BBB integrity and neurological function recovery after ICH by inhibiting Rho A/ROCK Ⅱ/MLC 2 signaling pathway activation and by regulating endothelial cell–related proteins. 展开更多
关键词 blood-brain barrier CAVEOLAE INTRACEREBRAL hemorrhage RhoA/rock II/MLC 2 signaling pathway SCALP ACUPUNCTURE
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Correlation between the rock mass properties and maximum horizontal stress:A case study of overcoring stress measurements
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作者 Peng Li Meifeng Cai +2 位作者 Shengjun Miao Yuan Li Yu Wang 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS 2025年第1期39-48,共10页
Understanding the mechanical properties of the lithologies is crucial to accurately determine the horizontal stress magnitude.To investigate the correlation between the rock mass properties and maximum horizontal stre... Understanding the mechanical properties of the lithologies is crucial to accurately determine the horizontal stress magnitude.To investigate the correlation between the rock mass properties and maximum horizontal stress,the three-dimensional(3D)stress tensors at 89 measuring points determined using an improved overcoring technique in nine mines in China were adopted,a newly defined characteristic parameter C_(ERP)was proposed as an indicator for evaluating the structural properties of rock masses,and a fuzzy relation matrix was established using the information distribution method.The results indicate that both the vertical stress and horizontal stress exhibit a good linear growth relationship with depth.There is no remarkable correlation between the elastic modulus,Poisson's ratio and depth,and the distribution of data points is scattered and messy.Moreover,there is no obvious relationship between the rock quality designation(RQD)and depth.The maximum horizontal stress σ_(H) is a function of rock properties,showing a certain linear relationship with the C_(ERP)at the same depth.In addition,the overall change trend of σ_(H) determined by the established fuzzy identification method is to increase with the increase of C_(ERP).The fuzzy identification method also demonstrates a relatively detailed local relationship betweenσ_H and C_(ERP),and the predicted curve rises in a fluctuating way,which is in accord well with the measured stress data. 展开更多
关键词 overcoring stress measurements elastic modulus Poisson's ratio rock quality designation maximum horizontal stress fuzzy identification
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SLC9A3-AS1调控miR-148a-3p/ROCK1信号轴影响肾癌细胞生物学功能 被引量:1
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作者 向威 吕磊 +2 位作者 郑福鑫 章传华 袁敬东 《华中科技大学学报(医学版)》 CAS CSCD 北大核心 2024年第2期161-167,共7页
目的检测lncRNA SLC9A3-AS1在肾透明细胞癌(clear cell renal cell carcinoma,ccRCC)组织与肾癌细胞中的表达水平,探讨其促进肾癌细胞恶性生物学行为的机制。方法应用GEPIA2在线软件(http://gepia2.cancer-pku.cn/)分析TCGA数据库中SLC9... 目的检测lncRNA SLC9A3-AS1在肾透明细胞癌(clear cell renal cell carcinoma,ccRCC)组织与肾癌细胞中的表达水平,探讨其促进肾癌细胞恶性生物学行为的机制。方法应用GEPIA2在线软件(http://gepia2.cancer-pku.cn/)分析TCGA数据库中SLC9A3-AS1在ccRCC组织中的表达水平;采用实时荧光定量聚合酶链反应(qRT-PCR)检测SLC9A3-AS1在不同肾癌细胞系、24例ccRCC组织与癌旁正常肾脏组织中的表达水平;应用细胞增殖/毒性检测试剂盒(cell counting kit-8,CCK-8)和Transwell小室迁移实验检测敲低SLC9A3-AS1表达对肾癌细胞增殖与迁移的影响;应用蛋白质免疫印迹法检测增殖与迁移相关信号通路蛋白的表达水平;采用双荧光素酶报告基因实验验证SLC9A3-AS1与miR-148a-3p/ROCK1轴的靶向调控关系。结果GEPIA2软件分析结果显示,相较正常肾脏组织,SLC9A3-AS1在肾透明细胞癌组织中表达显著上调(P<0.01)。qRT-PCR结果显示,相较癌旁正常肾脏组织,SLC9A3-AS1在24例ccRCC组织中表达显著上调(P<0.01);相较永生化肾小管上皮细胞,SLC9A3-AS1在4种肾癌细胞系中表达均显著上调,以786-O细胞最为显著(P<0.01)。干扰SLC9A3-AS1表达,可显著抑制786-O细胞的增殖与迁移能力,上调E-cadherin的蛋白表达水平,而下调N-cadherin、MMP2的蛋白表达水平(均P<0.05);过表达miR-148a-3p可显著抑制786-O细胞的增殖与迁移能力(P<0.01)。双荧光素酶报告基因检测结果表明,SLC9A3-AS1可特异性结合miR-148a-3p,后者进一步靶向结合ROCK1 mRNA的3′UTR区域;过表达miR-148a-3p可明显下调ROCK1 mRNA的表达水平,敲低miR-148a-3p表达则产生相反的效应;敲低SLC9A3-AS1表达可显著下调786-O细胞中ROCK1 mRNA与蛋白的表达水平(P<0.01);下调miR-148a-3p表达可部分逆转SLC9A3-AS1沉默对786-O细胞增殖与迁移的抑制作用(P<0.05)。结论SLC9A3-AS1在ccRCC中通过调控miR-148a-3p/ROCK1轴发挥促癌作用,有望成为ccRCC的一个新的分子标志物。 展开更多
关键词 肾透明细胞癌 SLC9A3-AS1 miR-148a-3p rock1
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贝母素乙调节RhoA/ROCK信号通路对膝骨关节炎大鼠软骨损伤的影响
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作者 彭博文 冯立 覃剑 《中国骨质疏松杂志》 CAS CSCD 北大核心 2024年第7期980-984,共5页
目的 探讨贝母素乙调节Ras同源基因家族成员A(RhoA)/Rho相关的卷曲螺旋激酶(ROCK)信号通路对膝骨关节炎(knee osteoarthritis, KOA)大鼠软骨损伤的影响。方法 以碘乙酸钠(MIA)法制备KOA大鼠模型,随机分为模型组、贝母素乙(3.5 mg/kg)组... 目的 探讨贝母素乙调节Ras同源基因家族成员A(RhoA)/Rho相关的卷曲螺旋激酶(ROCK)信号通路对膝骨关节炎(knee osteoarthritis, KOA)大鼠软骨损伤的影响。方法 以碘乙酸钠(MIA)法制备KOA大鼠模型,随机分为模型组、贝母素乙(3.5 mg/kg)组、贝母素乙(3.5 mg/kg)+LPA(RhoA激活剂,1 mg/kg)组,每组10只,另选10只大鼠为对照组。机械刺激法和热辐射法检测大鼠膝关节疼痛症状;检测大鼠关节肿胀度、膝关节宽度并以步态评分评测其关节功能;透射电镜检测大鼠膝关节软骨细胞超微结构;TUNEL染色检测大鼠关节软骨细胞凋亡比;酶联免疫吸附(ELISA)法检测大鼠血清和关节液促炎因子白细胞介素(IL)-18、单核细胞趋化蛋白1(MCP-1)水平;免疫印迹检测各组大鼠关节软骨组织凋亡(Cleaved caspase-3、Bax)和RhoA/ROCK通路相关蛋白表达。结果 贝母素乙可降低KOA大鼠关节肿胀度、膝关节宽度、步态评分、软骨细胞凋亡比、血清及关节液IL-18、MCP-1水平、软骨组织Cleaved caspase-3、Bax、RhoA、ROCK1、ROCK1蛋白表达,升高机械痛阈值、热痛阈值(P<0.05);LPA可减弱贝母素乙对KOA大鼠关节软骨损伤的改善作用。结论 贝母素乙可通过抑制RhoA/ROCK信号激活而抑制KOA大鼠关节炎症反应和关节软骨细胞凋亡,减轻软骨损伤。 展开更多
关键词 贝母素乙 RhoA/rock 膝骨关节炎 软骨损伤 信号通路
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益母草碱调节RhoA/ROCK信号通路对乙型肝炎大鼠的治疗作用
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作者 王维 穆宝龙 +3 位作者 张文双 吴清雷 张慧慧 曹智丽 《广州中医药大学学报》 CAS 2024年第12期3259-3265,共7页
【目的】观察益母草碱对乙型肝炎大鼠的治疗作用及机制。【方法】将60只大鼠随机分为6组,即正常组,模型组,益母草碱低、中、高剂量组,益母草碱高剂量+溶血磷脂酸(LPA)组。除正常组外,其余各组大鼠构建乙型肝炎病毒(HBV)感染模型。造模... 【目的】观察益母草碱对乙型肝炎大鼠的治疗作用及机制。【方法】将60只大鼠随机分为6组,即正常组,模型组,益母草碱低、中、高剂量组,益母草碱高剂量+溶血磷脂酸(LPA)组。除正常组外,其余各组大鼠构建乙型肝炎病毒(HBV)感染模型。造模成功后,各组对应干预。干预结束后,采用酶联免疫吸附分析(ELISA)检测血清乙型肝炎e抗原(HBeAg)、乙型肝炎表面抗原(HBsAg)及炎症因子白细胞介素(IL)-10、IL-6、肿瘤坏死因子α(TNF-α)水平,自动分析仪测定血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)水平,实时定量聚合酶链反应(RT-qPCR)法检测肝组织HBV DNA水平,苏木素-伊红(HE)染色法观察肝组织病理变化,免疫组织化学法检测肝组织乙型肝炎核心抗原(HBcAg)表达水平,Western Blot法检测肝组织Ras同源基因家族成员A(RhoA)、Rho相关卷曲螺旋蛋白激酶(ROCK)1、ROCK2表达水平。【结果】与正常组比较,模型组大鼠肝小叶中心坏死,肝细胞水肿、变性,HBsAg、HBeAg、HBV DNA水平,HBcAg阳性细胞数,ALT、AST水平,IL-6、TNF-α、IL-10含量,RhoA、ROCK1、ROCK2表达水平均显著升高(均P<0.05);与模型组比较,益母草碱低、中、高剂量组大鼠肝损伤程度降低,肝细胞变性减轻,HBsAg、HBeAg、HBV DNA水平,HBcAg阳性细胞数,ALT、AST水平,IL-6、TNF-α含量,RhoA、ROCK1、ROCK2表达水平均显著降低,IL-10含量升高,差异均有统计学意义(P<0.05);与益母草碱高剂量组比较,益母草碱高剂量+LPA组肝损伤程度加重,HBsAg、HBeAg、HBV DNA水平,HBcAg阳性细胞数,ALT、AST水平,IL-6、TNF-α含量,RhoA、ROCK1、ROCK2表达水平升高,IL-10含量降低,差异均有统计学意义(P<0.05)。【结论】益母草碱能减轻乙型肝炎大鼠肝组织损伤,改善肝功能,减少炎症反应,其机制与抑制RhoA/ROCK通路有关。 展开更多
关键词 益母草碱 乙型肝炎 肝功能 炎症反应 RhoA/rock通路 大鼠
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桃叶珊瑚苷调节RhoA/ROCK信号通路对胶质母细胞瘤细胞活力和上皮间质转化的影响
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作者 李娟 石海平 李维民 《天津中医药》 CAS 2024年第3期386-392,共7页
[目的]研究桃叶珊瑚苷(AU)对胶质母细胞瘤(GBM)细胞活力和上皮间质转化(EMT)的影响,并对其作用机制进行探讨。[方法]将U87细胞随机分为对照组、AU低浓度组、AU中浓度组、AU高浓度组、Y-27632组、AU高浓度+Y-27632组。细胞计数器试剂盒(C... [目的]研究桃叶珊瑚苷(AU)对胶质母细胞瘤(GBM)细胞活力和上皮间质转化(EMT)的影响,并对其作用机制进行探讨。[方法]将U87细胞随机分为对照组、AU低浓度组、AU中浓度组、AU高浓度组、Y-27632组、AU高浓度+Y-27632组。细胞计数器试剂盒(CCK-8)法检测细胞活力,流式细胞术检测细胞凋亡,Transwell小室实验检测细胞迁移和侵袭,蛋白免疫印迹法(Western Blot)检测基质金属蛋白酶(MMP)2、MMP9、波形蛋白(Vimentin)、上皮钙黏蛋白(E-cadherin)、神经钙黏蛋白(N-cadherin)、Ras同源基因家族成员A(RhoA)、Rho相关卷曲螺旋蛋白激酶(ROCK)1、ROCK2表达。构建GBM裸鼠模型,随机分为裸鼠对照组、AU组、Y-27632组、AU+Y-27632组,测量肿瘤质量与体积,免疫组化法检测移植瘤组织RhoA、ROCK1、ROCK2蛋白表达。[结果]GBM细胞活力随着AU浓度的升高而逐渐降低(P<0.05),选择U87作为后续实验细胞,选择10、25、50μmol/L浓度作为AU后续实验浓度。与对照组比较,AU低、中、高浓度组和Y-27632组细胞活力、迁移和侵袭细胞数、MMP2、MMP9、N-cadherin、Vimentin、RhoA、ROCK1、ROCK2表达显著下降,凋亡率、E-cadherin表达显著升高(P<0.05),其中高浓度AU和Y-27632组共同处理的细胞变化更显著(P<0.05)。AU和Y-27632均能抑制移植瘤质量和体积,降低RhoA/ROCK信号通路蛋白表达(P<0.05)。[结论]AU能抑制GBM细胞活力、迁移侵袭和EMT,促进细胞凋亡,其作用机制可能与抑制RhoA/ROCK信号通路有关。 展开更多
关键词 胶质母细胞瘤 细胞活力 上皮间质转化 桃叶珊瑚苷 RhoA/rock信号通路
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Explicit reinforcement models for fully-grouted rebar rock bolts 被引量:13
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作者 Navid Bahrani John Hadjigeorgiou 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2017年第2期267-280,共14页
This paper investigates the explicit use of rock reinforcement in a discontinuous stress analysis model.A series of numerical experiments was undertaken to evaluate the performance of local and global reinforcement mo... This paper investigates the explicit use of rock reinforcement in a discontinuous stress analysis model.A series of numerical experiments was undertaken to evaluate the performance of local and global reinforcement models implemented in universal distinct element code(UDEC).This was made possible by calibrating the reinforcement models to the laboratory behavior of a fully-grouted rebar bolt tested under pure pull and pure shear loading conditions.The model calibration focuses on matching different loading stages of the force-displacement curve including the initial elastic response,the hardening behavior and the bolt rupture.The paper concludes with a discussion on the suitability of the different reinforcement models in UDEC including their advantages and limitations.Finally,it addresses the choice of input parameters required for a realistic simulation of fully-grouted rebar bolts. 展开更多
关键词 rock reinforcement Fully-grouted rebar Distinct element method Local reinforcement Global reinforcement Pull-out test Shear test
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芍药苷调节RhoA/ROCK信号通路对实验性自身免疫性葡萄膜炎小鼠Th17/Treg免疫平衡的影响 被引量:2
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作者 庞彬彬 陈震 邢怡桥 《中药新药与临床药理》 CAS CSCD 北大核心 2024年第4期506-512,共7页
目的探讨芍药苷调节Ras同源基因家族蛋白A(RhoA)/Rho相关卷曲螺旋形成蛋白激酶(ROCK)信号通路对实验性自身免疫性葡萄膜炎(experimental autoimmune uveitis,EAU)小鼠辅助性T淋巴细胞17(Th17)/调节性T细胞(Treg)免疫平衡的影响。方法随... 目的探讨芍药苷调节Ras同源基因家族蛋白A(RhoA)/Rho相关卷曲螺旋形成蛋白激酶(ROCK)信号通路对实验性自身免疫性葡萄膜炎(experimental autoimmune uveitis,EAU)小鼠辅助性T淋巴细胞17(Th17)/调节性T细胞(Treg)免疫平衡的影响。方法随机选出10只小鼠作为正常对照组,其余小鼠构建EAU模型,将造模成功小鼠随机平分为模型组、芍药苷组(10 mg·kg^(-1)芍药苷)、RhoA/ROCK信号通路激活剂溶血磷脂酸(LPA)组(25μmol·L^(-1)LPA)、芍药苷+LPA组(10 mg·kg^(-1)芍药苷+25μmol·L^(-1)LPA),每组10只。模型组和正常对照组给予等量生理盐水,给药每天1次,持续14 d。眼前节临床表现评分评估炎症严重程度;HE染色观察眼球组织病理形态;ELISA法检测血清中炎性因子转化生长因子-β(trarsforming growth factor beta,TGF-β)、白细胞介素10(IL-10)、白细胞介素17(IL-17)、白细胞介素23(IL-23)水平;流式细胞术检测脾脏Th17/Treg细胞水平;Western Blot法检测维甲酸相关核孤儿受体γt(RORγt)、叉头状转录因子3(Foxp3)以及RhoA/ROCK信号通路蛋白表达。结果正常对照组小鼠无虹膜、结膜血管舒张充血,与正常对照组比,模型组小鼠出现虹膜血管舒张充血,前房、虹膜、睫状体和视网膜内均有大量炎性细胞膨胀,眼前节临床表现评分、IL-17和IL-23含量、Th17细胞水平、Th17/Treg、RORγt、RhoA、ROCK1蛋白水平明显升高(P<0.05),IL-10和TGF-β含量、Treg细胞水平、Foxp3水平明显下降(P<0.05)。与模型组比,芍药苷组小鼠虹膜血管充血症状明显缓解,眼前节临床表现评分、IL-17和IL-23含量、Th17细胞水平、Th17/Treg、RORγt、RhoA、ROCK1蛋白水平明显降低(P<0.05),IL-10和TGF-β含量、Treg细胞水平、Foxp3水平明显升高(P<0.05);而LPA加重了眼部损伤(P<0.05),逆转了芍药苷对EAU小鼠的改善效果(P<0.05)。结论芍药苷可通过下调RhoA/ROCK信号通路调节EAU小鼠Th17/Treg免疫平衡减轻EAU小鼠葡萄膜炎。 展开更多
关键词 芍药苷 RhoA/rock信号通路 自身免疫性葡萄膜炎 TH17/TREG 小鼠
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基于RhoA/ROCK2通路探讨七味白术散对糖尿病脑病大鼠学习记忆与突触可塑性的影响 被引量:1
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作者 尹怡然 冯晓帆 +4 位作者 高佳馨 王建波 张云雨 郑一 于海航 《时珍国医国药》 CAS CSCD 北大核心 2024年第1期56-59,共4页
目的 探讨七味白术散对糖尿病模型大鼠海马组织RhoA/ROCK2通路的作用,并探讨其改善糖尿病脑病的作用机制。方法 以高脂饲料结合STZ复制并筛选出符合条件的DE大鼠模型,随机分为模型组、七味白术散低、中、高剂量组、西药组。另设不造模... 目的 探讨七味白术散对糖尿病模型大鼠海马组织RhoA/ROCK2通路的作用,并探讨其改善糖尿病脑病的作用机制。方法 以高脂饲料结合STZ复制并筛选出符合条件的DE大鼠模型,随机分为模型组、七味白术散低、中、高剂量组、西药组。另设不造模的正常组。采用Morris水迷宫实验观察各组大鼠学习和空间记忆能力,用ELISA法检测海马组织中β-淀粉样蛋白42(β-amyloid protein 42,Aβ42)与磷酸化Tau蛋白(p-tau)含量,用RT-PCR法和Western blot检测海马样本中突触相关蛋白Syn、突触后致密物质PSD95(Postsynaptic density protein-95,PSD95)、RhoA、ROCK2的表达水平。结果 在检测各海马样本中Syn、PSD95、RhoA及ROCK2相对表达后,发现七味白术散干预4周后,模型组与正常组比较:RhoA、ROCK2表达水平显著升高,Syn、PSD95表达水平显著下降,差异有统计学意义(P<0.01),提示DE大鼠海马组织中的RhoA/ROCK2信号通路被抑制。中剂量组与模型组比较后发现:RhoA、ROCK2表达水平显著下降,Syn、PSD95表达水平显著上升,差异有统计学意义(P<0.01),表明七味白术散可能通过抑制RhoA/ROCK2通路,并调节突触可塑性。结论 七味白术散可能是通过抑制RhoA/ROCK2信号通路并上调突触可塑性来改善患有DE的大鼠。 展开更多
关键词 糖尿病脑病 RhoA/rock2通路 突触可塑性 七味白术散
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Dynamic rock tests using split Hopkinson (Kolsky) bar system - A review 被引量:89
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作者 Kaiwen Xia Wei Yao 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2015年第1期27-59,共33页
Dynamic properties of rocks are important in a variety of rock mechanics and rock engineering problems. Due to the transient nature of the loading, dynamic tests of rock materials are very different from and much more... Dynamic properties of rocks are important in a variety of rock mechanics and rock engineering problems. Due to the transient nature of the loading, dynamic tests of rock materials are very different from and much more challenging than their static counterparts. Dynamic tests are usually conducted using the split Hopkinson bar or Kolsl^j bar systems, which include both split Hopkinson pressure bar (SHPB) and split Hopkinson tension bar (SHTB) systems. Significant progress has been made on the quantification of various rock dynamic properties, owing to the advances in the experimental techniques of SHPB system. This review aims to fully describe and critically assess the detailed procedures and principles of tech- niques for dynamic rock tests using split Hopkinson bars. The history and principles of SHPB are outlined, followed by the key loading techniques that are useful for dynamic rock tests with SHPB (i.e. pulse shaping, momentum-trap and multi-axial loading techniques). Various measurement techniques for rock tests in SHPB (i.e. X-ray micro computed tomography (CT), laser gap gauge (LGG), digital image corre- lation (DIC), Moir~ method, caustics method, photoelastic coating method, dynamic infrared thermog- raphy) are then discussed. As the main objective of the review, various dynamic measurement techniques for rocks using SHPB are described, including dynamic rock strength measurements (i.e. dynamic compression, tension, bending and shear tests), dynamic fracture measurements (i.e. dynamic imitation and propagation fracture toughness, dynamic fracture energy and fracture velocity), and dy- namic techniques for studying the influences of temperature and pore water. 展开更多
关键词 rock Split Hopkinson pressure bar (SHPB) Dynamic testsrock dynamic properties Loading rate
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Nonlinear shear behavior of rock joints using a linearized implementation of the Barton-Bandis model 被引量:8
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作者 Simon Heru Prassetyo Marte Gutierrez Nick Barton 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2017年第4期671-682,共12页
Experiments on rock joint behaviors have shown that joint surface roughness is mobilized under shearing,inducing dilation and resulting in nonlinear joint shear strength and shear stress vs.shear displacement behavior... Experiments on rock joint behaviors have shown that joint surface roughness is mobilized under shearing,inducing dilation and resulting in nonlinear joint shear strength and shear stress vs.shear displacement behaviors.The Barton-Bandis(B-B) joint model provides the most realistic prediction for the nonlinear shear behavior of rock joints.The B-B model accounts for asperity roughness and strength through the joint roughness coefficient(JRC) and joint wall compressive strength(JCS) parameters.Nevertheless,many computer codes for rock engineering analysis still use the constant shear strength parameters from the linear Mohr-Coulomb(M-C) model,which is only appropriate for smooth and non-dilatant joints.This limitation prevents fractured rock models from capturing the nonlinearity of joint shear behavior.To bridge the B-B and the M C models,this paper aims to provide a linearized implementation of the B-B model using a tangential technique to obtain the equivalent M-C parameters that can satisfy the nonlinear shear behavior of rock joints.These equivalent parameters,namely the equivalent peak cohesion,friction angle,and dilation angle,are then converted into their mobilized forms to account for the mobilization and degradation of JRC under shearing.The conversion is done by expressing JRC in the equivalent peak parameters as functions of joint shear displacement using proposed hyperbolic and logarithmic functions at the pre-and post-peak regions of shear displacement,respectively.Likewise,the pre-and post-peak joint shear stiffnesses are derived so that a complete shear stress-shear displacement relationship can be established.Verifications of the linearized implementation of the B-B model show that the shear stress-shear displacement curves,the dilation behavior,and the shear strength envelopes of rock joints are consistent with available experimental and numerical results. 展开更多
关键词 rock joints Joint shear behavior Friction and dilation barton-Bandis(B-B) model Equivalent Mohr-Coulomb(M-C) parameters
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Relationship between diameter of split Hopkinson pressure bar and minimum loading rate under rock failure 被引量:6
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作者 李夕兵 洪亮 +2 位作者 尹土兵 周子龙 叶洲元 《Journal of Central South University of Technology》 EI 2008年第2期218-223,共6页
In order to investigate the relationship between bar diameter and loading rate of the split Hopkinson pressure bar(SHPB) setup under the failure of rock specimen and realize the medium strain rate loading of specimen,... In order to investigate the relationship between bar diameter and loading rate of the split Hopkinson pressure bar(SHPB) setup under the failure of rock specimen and realize the medium strain rate loading of specimen,new SHPB setups with different elastic bar's diameters of 22,36,50 and 75 mm were constructed.The tests were carried out on these setups at different loading rates,and the specimens had the same diameter of elastic bars and same ratio of length to diameter.The test results show that the larger the elastic bar's diameter is,the less the loading rate is needed to cause specimen failure,they show good power relationship,and that under the same strain rate loading,specimens are broken more seriously with larger diameter SHPB setup than with smaller one. 展开更多
关键词 rock failure Hopkinson pressure bar DIAMETER minimum loading rate medium strain rate
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Partially decoupling and collar bonding of the encapsulated rebar rockbolts to improve their performance in seismic prone deep underground excavations 被引量:3
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作者 Reza Masoudi Mostafa Sharifzadeh Masoud Ghorbani 《International Journal of Mining Science and Technology》 SCIE EI CSCD 2019年第3期409-418,共10页
Rockbolt is widely employed all over the world as an effective ground reinforcement element in order to secure the underground workplaces.Ordinary encapsulated rebar or rebar rockbolt is most popular and commonly used... Rockbolt is widely employed all over the world as an effective ground reinforcement element in order to secure the underground workplaces.Ordinary encapsulated rebar or rebar rockbolt is most popular and commonly used as reinforcement in a ground support system because of its accessibility,cost effectiveness and easy practicability.Reinforcement elements in a seismic condition such as rock burst have to dissipate the energy release of the dynamic impact via their deformation and ultimate load capacity,knowing that the former is more important.In other words,achieving early stiff behaviour along with large deformation capacity in rockbolts are the goals for new development in rock reinforcement.Yielding rockbolts are expensive while some of them have large deformation capability with low ultimate load capacity.In this paper,modifications were made on encapsulation of rebar rockbolts to utilise it effectively as a yielding reinforcement in seismic conditions.Applying a sufficient decoupled length in the shank of rebar rockbolts which industry has regularly been using to control the bulking of the stress fractured ground,improves the deformation capacity of the bolt.Additionally,leaving a collar bonding underneath of the bearing pad and plate removes the weaknesses of the head anchorage of rockbolt.Therefore the dynamic performance of the bolt is improved by these easily applicable modifications.The behaviour and performance of encapsulated rockbolts have been discussed first,then the effects of modifications are illustrated.The proposed modification of the rebars is not only cost effective but also easy to apply in the field and improves the performance of reinforcements in seismic prone zones. 展开更多
关键词 rockbolt Tunnel SUPPORT COLLAR BONDING SEISMIC rock SUPPORT Tunnelling
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苍术素调节RhoA/ROCK信号通路对弥漫大B细胞淋巴瘤细胞恶性生物学行为的影响 被引量:1
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作者 张薇 陈姣敏 +2 位作者 许卫星 尹凤雷 王娟 《陕西医学杂志》 CAS 2024年第5期593-597,共5页
目的:探讨苍术素(ATR)调节Ras同源基因家族成员A(RhoA)/Rho相关卷曲螺旋形成蛋白激酶(ROCK)信号通路对弥漫大B细胞淋巴瘤(DLBCL)细胞恶性生物学行为的影响。方法:常规培养DLBCL细胞SUDHL-4,将其随机分为对照组、低浓度ATR组(ATR-L组,5μ... 目的:探讨苍术素(ATR)调节Ras同源基因家族成员A(RhoA)/Rho相关卷曲螺旋形成蛋白激酶(ROCK)信号通路对弥漫大B细胞淋巴瘤(DLBCL)细胞恶性生物学行为的影响。方法:常规培养DLBCL细胞SUDHL-4,将其随机分为对照组、低浓度ATR组(ATR-L组,5μmol/L ATR)、中浓度ATR组(ATR-M组,10μmol/L ATR)、高浓度ATR组(ATR-H组,20μmol/L ATR)和高浓度ATR+RhoA激活剂U46619组(ATR+U46619组,20μmol/L ATR+10 nmol/L U46619)。CCK-8法测定细胞增殖活性。划痕实验检测细胞迁移能力。Transwell实验测定细胞侵袭能力。流式细胞术检测细胞凋亡率。实时荧光定量PCR(RT-qPCR)法测定细胞中RhoA、ROCK1 mRNA表达。免疫印迹法(Western blot)测定各组细胞B细胞淋巴瘤-2(Bcl-2)、Bcl-2相关X蛋白(Bax)、RhoA、ROCK1蛋白表达。结果:与对照组比较,ATR-M组、ATR-H组SUDHL-4细胞OD450值(48、72 h)、细胞迁移率、细胞侵袭数目、RhoA、ROCK1 mRNA和蛋白表达、Bcl-2蛋白表达降低,细胞凋亡率、Bax蛋白表达升高(均P<0.05)。RhoA激活剂U46619减弱了ATR对DLBCL细胞恶性生物学行为的抑制作用。结论:ATR可能通过下调RhoA/ROCK信号通路抑制DLBCL细胞恶性生物学行为。 展开更多
关键词 弥漫大B细胞淋巴瘤 苍术素 RhoA/rock通路 细胞凋亡 细胞增殖 细胞侵袭 细胞迁移
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Controlled rocking pile foundation system with replaceable bar fuses for seismic resilience 被引量:3
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作者 Cheng Zhao Li Shuai Wang Jingquan 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2022年第3期683-696,共14页
Bridges designed following a conventional approach minimize the risk of collapse,but often require challenging,costly,and time-consuming restoration after an earthquake occurs.The new seismic design philosophy require... Bridges designed following a conventional approach minimize the risk of collapse,but often require challenging,costly,and time-consuming restoration after an earthquake occurs.The new seismic design philosophy requires bridges to maintain functionality even after severe earthquakes.In this context,this paper proposes a controlled rocking pile foundation(CRPF)system and numerically evaluates bridges′degree of seismic resilience.The CRPF system allows a pile cap to rock on a pile foundation and dissipate seismic energy through inelastic deformations of replaceable bar fuses that connect a pile cap and piles.Following the conceptual design of the CRPF system,two analytical models were developed for a bridge pier utilizing the CRPF system and a pier designed to develop a plastic hinge in its column.The analytical results indicate that,after experiencing a severe earthquake,a conventionally designed bridge pier sustained substantial damage in its column and exhibited significant residual displacement.In contrast,a pier using the CRPF system showed negligible residual displacement and maintained elastic behavior except,as expected,for bar fuses.The damaged fuses can be rapidly replaced to recover bridge seismic resistance following an earthquake.Therefore,the CRPF system helps to achieve the desired postearthquake performance objectives. 展开更多
关键词 seismic resilience bridges pier controlled rocking pile foundation repairable bar fuse
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Barton-Bandis criterion-based system reliability analysis of rock slopes 被引量:3
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作者 ZUO Shi HU Chang-wen +2 位作者 ZHAO Lian-heng ZHANG Ying-bin SONG Zhi-chen 《Journal of Central South University》 SCIE EI CAS CSCD 2020年第7期2123-2133,共11页
Several potential failure modes generally exist in rock slopes because of the existence of massive structural planes in rock masses. A system reliability analyses method for rock slopes with multiple failure modes bas... Several potential failure modes generally exist in rock slopes because of the existence of massive structural planes in rock masses. A system reliability analyses method for rock slopes with multiple failure modes based on nonlinear Barton-Bandis failure criterion is proposed. The factors of safety associated with the sliding and overturning failure modes are derived, respectively. The validity of this method is verified through a planar rock slope with an inclined slope top and tension crack. Several sensitivity analyses are adopted to study the influences of structural-plane parameters, geometric parameters, anchoring parameters and fracture morphology on the rock slopes system reliability. 展开更多
关键词 barton-Bandis criterion failure mode probability of failure rock slope system reliability analysis
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艾灸联合保真汤加减治疗糖尿病肾病的疗效观察及对CT灌注参数、Rho/ROCK信号通路蛋白的影响 被引量:1
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作者 赵小艳 王俊杰 《上海针灸杂志》 CSCD 2024年第1期17-23,共7页
目的 观察艾灸联合保真汤加减治疗糖尿病肾病气阴两虚证的临床疗效及对CT灌注参数、Rho/ROCK信号通路蛋白的影响。方法 将146例糖尿病肾病气阴两虚证患者随机分为观察组(74例)和对照组(72例)。在对症治疗的基础上,对照组给予厄贝沙坦片... 目的 观察艾灸联合保真汤加减治疗糖尿病肾病气阴两虚证的临床疗效及对CT灌注参数、Rho/ROCK信号通路蛋白的影响。方法 将146例糖尿病肾病气阴两虚证患者随机分为观察组(74例)和对照组(72例)。在对症治疗的基础上,对照组给予厄贝沙坦片,观察组给予艾灸联合保真汤加减。观察两组中医主症积分、CT灌注参数[血尿素氮(blood urea nitrogen, BUN)、血肌酐(serum creatinine, SCr)、尿白蛋白排泄率(urinary albumin excretion rate, UAER)和24 h尿蛋白定量(24-hour urinary protein quantification, 24 h Upro)]、肾血流指标[舒张末期血流速度(end-diastolic velocity, EDV)、肾段动脉的收缩期峰值流速(peak-systolic velocity, PSV)、搏动指数(pulsatility index, PI)和阻力指数(resistive index, RI)]、血清炎性因子[肿瘤坏死因子-α(tumor necrosis factor-α, TNF-α)、白细胞介素-1β(interleukin-1β, IL-1β)、白细胞介素-6(interleukin-6, IL-6)和C反应蛋白(C-reactive protein, CRP)]水平及Rho/ROCK信号通路[Ras同源基因家族成员A(Ras homolog gene family member A, RhoA)、Rho关联含卷曲螺旋蛋白激酶Ⅰ(Rho-associated coiled-coil containing protein kinaseⅠ, ROCKI)、α-平滑肌肌动蛋白(α-smooth muscle actin, α-SMA)和钙黏附蛋白-E(E-cadherin, E-Cad)]蛋白水平,并比较两组临床疗效及不良反应。结果 观察组总有效率为97.3%(72/74),明显高于对照组的81.9%(59/72)(P<0.05)。观察组治疗后中医主症积分低于治疗前和对照组(P<0.05)。两组治疗后CT灌注参数降低(P<0.05),且观察组低于对照组(P<0.05)。观察组治疗后PSV、EDV较治疗前和对照组加快(P<0.05),RI、PI较治疗前和对照组降低(P<0.05)。观察组治疗后血清炎性因子水平较治疗前和对照组降低(P<0.05)。观察组治疗后Rho A、ROCKI、α-SMA蛋白水平较治疗前和对照组降低(P<0.05),E-Cad蛋白水平较治疗前和对照组升高(P<0.05)。观察组不良反应发生率为1.4%(1/74),低于对照组的19.4%(14/72)(P<0.05)。结论 在对症治疗的基础上,艾灸联合保真汤加减可明显提高糖尿病肾病气阴两虚证患者的治疗效果,其机制可能与改善CT灌注参数,调节血清Rho/ROCK信号通路蛋白相关。 展开更多
关键词 灸法 糖尿病并发症 糖尿病肾病 气阴两虚 Rho/rock信号通路蛋白
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System reliability analysis of seismic pseudo-static stability of rock wedge based on nonlinear Barton–Bandis criterion 被引量:3
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作者 ZHAO Lian-heng JIAO Kang-fu +1 位作者 LI De-jian ZUO Shi 《Journal of Central South University》 SCIE EI CAS CSCD 2020年第11期3450-3463,共14页
Based on the nonlinear Barton–Bandis(B–B)failure criterion,this study considers the system reliability of rock wedge stability under the pseudo-static seismic load.The failure probability(Pf)of the system is calcula... Based on the nonlinear Barton–Bandis(B–B)failure criterion,this study considers the system reliability of rock wedge stability under the pseudo-static seismic load.The failure probability(Pf)of the system is calculated based on the Monte−Carlo method when considering parameter correlation and variability.Parameter analysis and sensitivity analysis are carried out to explore the influence of parameters on reliability.The relationships among the failure probability,safety factor(Fs),and variation coefficient are explored,and then stability probability curves of the rock wedge under the pseudo-static seismic load are drawn.The results show that the parameter correlation of the B–B failure criterion has a significant influence on the failure probability,but correlation increases system reliability or decreases system reliability affected by other parameters.Under the pseudo-static seismic action,sliding on both planes is the main failure mode of wedge system.In addition,the parameters with relatively high sensitivity are two angles related to the joint dip.When the coefficient of variation is consistent,the probability of system failure is a function of the safety factor. 展开更多
关键词 3D rock wedge seismic pseudo-static stability nonlinear barton–Bandis failure criterion system reliability sensitivity analysis stability probability curves
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黄芩苷通过ROS依赖性调节RhoA/ROCK通路保护氯化钴诱导心肌细胞损伤的实验研究 被引量:3
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作者 沈艳玲 刘承红 +3 位作者 王世魁 徐尧 张云波 顾申红 《海南医学院学报》 CAS 北大核心 2024年第7期481-488,共8页
目的:本研究旨在探讨黄芩苷(Baicalin)对氯化钴(CoCl_(2))诱导H9c2心肌细胞缺氧损伤的作用机制。方法:采用CoCl_(2)建立心肌细胞缺氧损伤模型,并分别加入不同浓度的黄芩苷培养。将正常氧培养设置为对照组,CoCl_(2)培养H9c2心肌细胞设置... 目的:本研究旨在探讨黄芩苷(Baicalin)对氯化钴(CoCl_(2))诱导H9c2心肌细胞缺氧损伤的作用机制。方法:采用CoCl_(2)建立心肌细胞缺氧损伤模型,并分别加入不同浓度的黄芩苷培养。将正常氧培养设置为对照组,CoCl_(2)培养H9c2心肌细胞设置为CoCl_(2)组;Baicalin、Y27632(Rho激酶抑制剂)预处理的H9c2缺氧心肌细胞设置为CoCl_(2)+Baicalin组、CoCl_(2)+Y27632组、CoCl_(2)+Baicalin+Y27632组。通过细胞毒性检测试剂盒(CCK8)检测细胞活性;荧光探针测定细胞中活性氧(ROS)的表达;WST-8检测心肌细胞超氧化歧化酶(SOD)活力;TBA法测定丙二醇(MDA)浓度;同时采用WesternBlot方法分析RhoA、ROCK1、ROCK2、TNF-α、IL-1β蛋白表达情况。结果:处理H9c2细胞24 h后,1 000μmol/L的CoCl_(2)和75μmol/L黄芩苷对治疗心肌细胞缺氧损伤具有较好的细胞活力。CoCl_(2)组细胞活性明显低于对照组,加入黄芩苷后可显著提高细胞活性(P<0.05);与对照组相比,CoCl_(2)组心肌细胞可上调ROS、MDA表达,下调SOD活力,升高RhoA、ROCK1、ROCK2、TNF-α、IL-1β蛋白表达水平,加入Baicalin后可逆转上诉蛋白的表达水平;与CoCl_(2)组相比,CoCl_(2)+Baicalin可抑制ROS、MDA的表达,上调SOD含量,下调RhoA、ROCK1、ROCK2、TNF-α、IL-1β蛋白表达水平,而加入Y27632(Rho激酶抑制剂)后则可显著增强Baicalin带来的保护作用。结论:Baicalin可减轻CoCl_(2)诱导H9c2心肌细胞缺氧损伤的炎症、氧化应激反应,其机制与抑制ROS依赖性RhoA/ROCK通路有关。 展开更多
关键词 黄芩苷 RhoA/Rho相关激酶(rock)信号通路 缺氧损伤模型
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