[ Objective] This study aimed to investigate the biological effects of laser-induced mutation on fibrous roots of yellow skin onion. [ Method] Wet seeds of two yellow skin onion cultivars were irradiated by CO2 laser ...[ Objective] This study aimed to investigate the biological effects of laser-induced mutation on fibrous roots of yellow skin onion. [ Method] Wet seeds of two yellow skin onion cultivars were irradiated by CO2 laser and He-Ne laser at three dosage levels separately. A randomized complete block design with three replications was adopted. The biological effects of laser-induced mutation on fibrous roots of Ll-generation yellow skin onion were investigated with biostatistics and physiological and biochemical methods. [Result] Significant variations in the biological effects caused by various laser treatments were observed in the length, quantity, fresh weight and activity of onion fibrous roots. Specifically, the variation in fibrous root length induced by different types of laser reached 5% significance level; significant variation was observed in fibrous roots of different onion cultivars induced by laser, while the variation among each treatment did not reach 5% sig- nifieance level ; the variation in fibrous root quantity induced by different dosage levels of laser reached 5% significance level ; laser radiation showed stimulating effect on root activity of onion. [ Conclusion] This study provided reference for laser-induced breeding of yellow skin onion.展开更多
Picea crassifolia Kom, a perennial arbor spe- cies is recognized as one of the most adaptable plants found to date in Qilian Mountains. To explore the cutting reproduction technology of P. crassifolia and reveal its r...Picea crassifolia Kom, a perennial arbor spe- cies is recognized as one of the most adaptable plants found to date in Qilian Mountains. To explore the cutting reproduction technology of P. crassifolia and reveal its rooting mechanism, cuttings of P. crassifolia with different cutting types （softwood, hardwood and root）, positions （top, upper, middle and bottom） and ages （7, 10, 15, 20, 25 year-old） were cultivated in a field experiment. One- year old softwood and hardwood cuttings were collected from 7-, 10-, 15-, 20-, and 25 year-old healthy ortets to analyze the changes from endogenous hormones and organic nutrients. Results indicate that the softwood cut- tings （0.5-1.0 cm in diameter） from upper branches of 15 year-old ortets shows better growth performance by improving rooting indexes, including a significant increasein rooting rate and a decrease in basal rot rate. Concomi- tantly, increasing rooting quantity and root length also increased. It is noteworthy that the high rooting rate of P. crassifolia cuttings due to its ability to accumulate high concentrations of indole-3-acetic acid （IAA） and total carbon （TC） rather than abscisic acid （ABA） and total nitrogen （TN）. The rooting rate was mainly regulated by the IAA/ABA and TC/TN ratio. In summary, our results suggest that the softwood cuttings （0.5-1.0 cm in diameter） from upper branches of 15 year-old P. crassifolia can be considered as an effective strategy to improve cutting rooting rate, and the IAA/ABA and TC/TN ratio was one of the main factors limiting the cutting rooting rate of P. crassifolia.展开更多
To reveal the period and after-effect of soil water stress on winter wheat, the article employs the experiment results carried out in the greenhouse. The results showed that the root-restricted weights varied with str...To reveal the period and after-effect of soil water stress on winter wheat, the article employs the experiment results carried out in the greenhouse. The results showed that the root-restricted weights varied with stress degrees and stress times during and after water stressing. In the course of stress, the chief reason resticting the weight of root was the stress intensity at this time, and that of severe stress treatment was larger than that of mild stress treatment. After water stress was relieved, the results of the after-effect of soil water stress on root growth were that, the stress intensity of short-time and mild stress was larger than that of long-time and severe stress. Comparing two-stage stress intensities, root-restricted weight resulted from after-effect intensity of stress under all of the short-time treatment, and the mild and the long-time stress treatments, while that resulted from the period stress intensity under the severe and the long-time stress treatments. In general, the effects of water stress on root were attributed to the three factors, a formed basis in the previous stage, the after-effect of water condition before this stage and influence of water in this stage, which lead to the characters of root in the whole growth stage.展开更多
Objective: To investigate the suitable time of treating virulent and side effects of chemotherapy for malignant tumor by acupoint injection of Astragalus Root injection. Methods: Sixty three patients with malignant tu...Objective: To investigate the suitable time of treating virulent and side effects of chemotherapy for malignant tumor by acupoint injection of Astragalus Root injection. Methods: Sixty three patients with malignant tumor were divided into three groups: prevention treatment (PT) group (n=23 cases),post chemotherapy treatment (PCT) group( n=22 cases), Western medicine (WM) group ( n=18 cases). The patients in PT, PCT and WM groups were treated respectively from the fifth day on before chemotherapy and from the first day on after chemotherapy, 18 days in all. The patients in WM group were administered Batilol and Leucogen from the first day on after chemotherapy. Changes of leukocytes and immunoglobulin before and after treatment were observed. Results: 1) Acupoint injection of Astragalus Root injectio could increase the number of leukocytes and immunoglobulin content and its effect was better than that of Western medicine (P<0.05); 2) The effect of PT group was better than that of PCT group in preventing and treating virulent and side effects of chemotherapy (P<0.05). Conclusion: It should be stressed on prevention of malignant tumor by using acupoint injection of Astragalus Root for relieving virulent and side effects of chemotherapy.展开更多
Recent climate changes, including an increase in precipitation, have affected tree physiology in eastern Siberia. We investigated the response of larch to wet and dry soil water conditions in pot experiments using lar...Recent climate changes, including an increase in precipitation, have affected tree physiology in eastern Siberia. We investigated the response of larch to wet and dry soil water conditions in pot experiments using larch seedlings grown under near-natural conditions in eastern Siberia over two growing seasons. Three patterns of wet- and dry-treatment combinations were applied over 2 years: wet treatments in 2006 and 2007 (WW treatment), dry in 2006 and wet in 2007 (DW treatment), and dry in 2006 and 2007 (DD treatment). After 1 year of treatment, no significant difference between the dry and wet treatment was found in root distribution and needle water content, except for the content of abscisic acid in roots. After 2 years of treatment, the DW treatment induced different tendencies in the gas exchange activity and in the needle biomass and root distribution of seedlings in comparison with WW treatments, despite the same water condition in 2007. We suggest a possibility that seedlings that experience drought stress might store some memory of drought that influences their physiology in the next growing season.展开更多
Traditional Chinese medicine Pseudostellaria heterophylla fibrous roots have been widely studied and applied in non-antibiotic clinical breeding of livestock and poultry. In vitro probiotic effect of P. heterophylla f...Traditional Chinese medicine Pseudostellaria heterophylla fibrous roots have been widely studied and applied in non-antibiotic clinical breeding of livestock and poultry. In vitro probiotic effect of P. heterophylla fibrous roots extract on feed probiotics Bacillus subtilis BD-K010 was studied, and the feasibility of solid fermentation of P. heterophylla fibrous roots was preliminarily evaluated. For in vitro probiotic effect, the increased concentration of P. heterophylla fibrous roots extract dependently increased the total biomass of B. subtilis BD-K010;1.0% P. heterophylla fibrous roots extract received the best effect, and the final p H of 1.0% experimental group was closer to neutral. Meantime, B. subtilis BD-K010 with optimum concentration of P. heterophylla fibrous roots showed significantly higher in vitro antibacterial effect than the control group(P<0.01),and the antibacterial effects on Escherichia coli and Staphylococcus aureus were improved by 51.99% and 63.16%, but it was ineffective to Salmonella. For solid fermentation, the profile of substrate complex and appendage flocculent structure on substrate surface at the end of fermenta-tion in experimental groups added with B. subtilis BD-K010 and cellulase plus BD-K010 were more complex;the live bacteria number, polysaccharide content and saponin content at fermentation end-point in two experimental groups were extremely higher than those in the control group(P<0.01).P. heterophylla fibrous roots extract had good in vitro probiotic effect on B. subtilis BD-K010 and promoted its antibacterial effect, and it is feasible to use probiotics for solid fermentation of P. heterophylla fibrous roots and to improve effective components. It is of great significance to further de-velop and utilize P. heterophylla fibrous roots resources in modern animal husbandry.展开更多
基金Supported by Natural Science Foundation of Sichuan Education Department(2008ZA033)
文摘[ Objective] This study aimed to investigate the biological effects of laser-induced mutation on fibrous roots of yellow skin onion. [ Method] Wet seeds of two yellow skin onion cultivars were irradiated by CO2 laser and He-Ne laser at three dosage levels separately. A randomized complete block design with three replications was adopted. The biological effects of laser-induced mutation on fibrous roots of Ll-generation yellow skin onion were investigated with biostatistics and physiological and biochemical methods. [Result] Significant variations in the biological effects caused by various laser treatments were observed in the length, quantity, fresh weight and activity of onion fibrous roots. Specifically, the variation in fibrous root length induced by different types of laser reached 5% significance level; significant variation was observed in fibrous roots of different onion cultivars induced by laser, while the variation among each treatment did not reach 5% sig- nifieance level ; the variation in fibrous root quantity induced by different dosage levels of laser reached 5% significance level ; laser radiation showed stimulating effect on root activity of onion. [ Conclusion] This study provided reference for laser-induced breeding of yellow skin onion.
基金supported by the National Natural Science Foundation of China(Grant No.31360086)the grant from the major state basic research development program of china(973 program,No.2013CB429903)
文摘Picea crassifolia Kom, a perennial arbor spe- cies is recognized as one of the most adaptable plants found to date in Qilian Mountains. To explore the cutting reproduction technology of P. crassifolia and reveal its rooting mechanism, cuttings of P. crassifolia with different cutting types （softwood, hardwood and root）, positions （top, upper, middle and bottom） and ages （7, 10, 15, 20, 25 year-old） were cultivated in a field experiment. One- year old softwood and hardwood cuttings were collected from 7-, 10-, 15-, 20-, and 25 year-old healthy ortets to analyze the changes from endogenous hormones and organic nutrients. Results indicate that the softwood cut- tings （0.5-1.0 cm in diameter） from upper branches of 15 year-old ortets shows better growth performance by improving rooting indexes, including a significant increasein rooting rate and a decrease in basal rot rate. Concomi- tantly, increasing rooting quantity and root length also increased. It is noteworthy that the high rooting rate of P. crassifolia cuttings due to its ability to accumulate high concentrations of indole-3-acetic acid （IAA） and total carbon （TC） rather than abscisic acid （ABA） and total nitrogen （TN）. The rooting rate was mainly regulated by the IAA/ABA and TC/TN ratio. In summary, our results suggest that the softwood cuttings （0.5-1.0 cm in diameter） from upper branches of 15 year-old P. crassifolia can be considered as an effective strategy to improve cutting rooting rate, and the IAA/ABA and TC/TN ratio was one of the main factors limiting the cutting rooting rate of P. crassifolia.
基金the Ministry of Science and Technology of China （1999011700） the National Natural Science Foundation of China （49971042）.
文摘To reveal the period and after-effect of soil water stress on winter wheat, the article employs the experiment results carried out in the greenhouse. The results showed that the root-restricted weights varied with stress degrees and stress times during and after water stressing. In the course of stress, the chief reason resticting the weight of root was the stress intensity at this time, and that of severe stress treatment was larger than that of mild stress treatment. After water stress was relieved, the results of the after-effect of soil water stress on root growth were that, the stress intensity of short-time and mild stress was larger than that of long-time and severe stress. Comparing two-stage stress intensities, root-restricted weight resulted from after-effect intensity of stress under all of the short-time treatment, and the mild and the long-time stress treatments, while that resulted from the period stress intensity under the severe and the long-time stress treatments. In general, the effects of water stress on root were attributed to the three factors, a formed basis in the previous stage, the after-effect of water condition before this stage and influence of water in this stage, which lead to the characters of root in the whole growth stage.
文摘Objective: To investigate the suitable time of treating virulent and side effects of chemotherapy for malignant tumor by acupoint injection of Astragalus Root injection. Methods: Sixty three patients with malignant tumor were divided into three groups: prevention treatment (PT) group (n=23 cases),post chemotherapy treatment (PCT) group( n=22 cases), Western medicine (WM) group ( n=18 cases). The patients in PT, PCT and WM groups were treated respectively from the fifth day on before chemotherapy and from the first day on after chemotherapy, 18 days in all. The patients in WM group were administered Batilol and Leucogen from the first day on after chemotherapy. Changes of leukocytes and immunoglobulin before and after treatment were observed. Results: 1) Acupoint injection of Astragalus Root injectio could increase the number of leukocytes and immunoglobulin content and its effect was better than that of Western medicine (P<0.05); 2) The effect of PT group was better than that of PCT group in preventing and treating virulent and side effects of chemotherapy (P<0.05). Conclusion: It should be stressed on prevention of malignant tumor by using acupoint injection of Astragalus Root for relieving virulent and side effects of chemotherapy.
文摘Recent climate changes, including an increase in precipitation, have affected tree physiology in eastern Siberia. We investigated the response of larch to wet and dry soil water conditions in pot experiments using larch seedlings grown under near-natural conditions in eastern Siberia over two growing seasons. Three patterns of wet- and dry-treatment combinations were applied over 2 years: wet treatments in 2006 and 2007 (WW treatment), dry in 2006 and wet in 2007 (DW treatment), and dry in 2006 and 2007 (DD treatment). After 1 year of treatment, no significant difference between the dry and wet treatment was found in root distribution and needle water content, except for the content of abscisic acid in roots. After 2 years of treatment, the DW treatment induced different tendencies in the gas exchange activity and in the needle biomass and root distribution of seedlings in comparison with WW treatments, despite the same water condition in 2007. We suggest a possibility that seedlings that experience drought stress might store some memory of drought that influences their physiology in the next growing season.
基金Supported by Innovation Fund Project of Fujian Provincial Department of Science and Technology(2018C0041)Soft Science Project of Fujian Provincial Intellectual Property Office(2018ER019)
文摘Traditional Chinese medicine Pseudostellaria heterophylla fibrous roots have been widely studied and applied in non-antibiotic clinical breeding of livestock and poultry. In vitro probiotic effect of P. heterophylla fibrous roots extract on feed probiotics Bacillus subtilis BD-K010 was studied, and the feasibility of solid fermentation of P. heterophylla fibrous roots was preliminarily evaluated. For in vitro probiotic effect, the increased concentration of P. heterophylla fibrous roots extract dependently increased the total biomass of B. subtilis BD-K010;1.0% P. heterophylla fibrous roots extract received the best effect, and the final p H of 1.0% experimental group was closer to neutral. Meantime, B. subtilis BD-K010 with optimum concentration of P. heterophylla fibrous roots showed significantly higher in vitro antibacterial effect than the control group(P<0.01),and the antibacterial effects on Escherichia coli and Staphylococcus aureus were improved by 51.99% and 63.16%, but it was ineffective to Salmonella. For solid fermentation, the profile of substrate complex and appendage flocculent structure on substrate surface at the end of fermenta-tion in experimental groups added with B. subtilis BD-K010 and cellulase plus BD-K010 were more complex;the live bacteria number, polysaccharide content and saponin content at fermentation end-point in two experimental groups were extremely higher than those in the control group(P<0.01).P. heterophylla fibrous roots extract had good in vitro probiotic effect on B. subtilis BD-K010 and promoted its antibacterial effect, and it is feasible to use probiotics for solid fermentation of P. heterophylla fibrous roots and to improve effective components. It is of great significance to further de-velop and utilize P. heterophylla fibrous roots resources in modern animal husbandry.
