Objective: To explore the molecular biological mechanism of Arnebia root oil promoting wound surface healing by observing histological change and bFGF expression in wound surface tissue as well as wound surface healin...Objective: To explore the molecular biological mechanism of Arnebia root oil promoting wound surface healing by observing histological change and bFGF expression in wound surface tissue as well as wound surface healing rate. Methods: Raw surface in patients were randomly divided into 2 groups. Experimental group was treated by Arnebia root oil and control group was treated by petrolatum gauze, then the tissular structure of raw surface was observed by histology, histochemistry. electron microscope and raw surface healing rates was compared either. bFGF expression in wound surface tissue was also evaluated by Western-blot. Results: Raw surface healing rate of experimental group and control group had obvious difference(P<0. 05). Raw surface of experimental group had more fibroblast, collagen and blood capillary. bFGF was expressed in both groups, and the level of bFGF expression in experimental group was higher than that in control group in every period. There were significant differences between 2 groups in gray-density value ( P<0. 05). Being as an internal control, no significant change was found for β-actin expression, although it occured in various phases. Conclusion: Arnebia root oil plays an important regulative role in the course of healing of wound and it can promote skin raw surface repair and accelerate wound surface healing, which are caused by enhancing bFGF in the wound tissue.展开更多
Zingiber rubens Roxb., and Zingiber zerumbet (L.) Smith were collected respectively from Nghe An, Province and Ha Tinh, Province,Vietnam. The root essential oils were obtained by water distillation and analyzed by Gas...Zingiber rubens Roxb., and Zingiber zerumbet (L.) Smith were collected respectively from Nghe An, Province and Ha Tinh, Province,Vietnam. The root essential oils were obtained by water distillation and analyzed by Gas chromatography (GC) and Gas chromatography coupled with Mass spectrometry (GC-MS). The most abundant components of Z. rubens were (Z)-citral (30.1%), camphene (9.7%), β-phellandrene (7.5%) and 1,8-cineole (7.0%) and zingiberene (5.3%). The main oil constituents of Z. zerumbet were (Z)-citral (26.1%), camphene (16.3%), sabinene (14.6%), zingiberene (7.2%) and lavandulyl acetate (6.7%). This species has low zerumbone (1.2%) content.展开更多
[Objectives] Volatile oil components of traditional drug use site " root and rhizome" of N. jatamansi and herb were identified and contrasted,which aimed to provide the basis for rationally using wild N. jat...[Objectives] Volatile oil components of traditional drug use site " root and rhizome" of N. jatamansi and herb were identified and contrasted,which aimed to provide the basis for rationally using wild N. jatamansi resource. [Methods]Volatile oil components from different sites of N. jatamansi were identified and isolated by GC-MS. [Results] There were 56 kinds of volatile oil components from different sites of N. jatamansi,in which 39 components from herb of N. jatamansi,39 components from root and rhizome of N. jatamansi,and there were 22 common components. But 1 component in higher concentration had obvious difference. [Conclusions] The herb of N. jatamansi could not completely replace " root and rhizome" of N. jatamansi as the medicine,which was consistent with prior detection result of each physicochemical index from different sites of N. jatamansi. The research could provide the reference for making quality standard of N. jatamansi medicine and rational basis for its further research and development.展开更多
文摘Objective: To explore the molecular biological mechanism of Arnebia root oil promoting wound surface healing by observing histological change and bFGF expression in wound surface tissue as well as wound surface healing rate. Methods: Raw surface in patients were randomly divided into 2 groups. Experimental group was treated by Arnebia root oil and control group was treated by petrolatum gauze, then the tissular structure of raw surface was observed by histology, histochemistry. electron microscope and raw surface healing rates was compared either. bFGF expression in wound surface tissue was also evaluated by Western-blot. Results: Raw surface healing rate of experimental group and control group had obvious difference(P<0. 05). Raw surface of experimental group had more fibroblast, collagen and blood capillary. bFGF was expressed in both groups, and the level of bFGF expression in experimental group was higher than that in control group in every period. There were significant differences between 2 groups in gray-density value ( P<0. 05). Being as an internal control, no significant change was found for β-actin expression, although it occured in various phases. Conclusion: Arnebia root oil plays an important regulative role in the course of healing of wound and it can promote skin raw surface repair and accelerate wound surface healing, which are caused by enhancing bFGF in the wound tissue.
文摘Zingiber rubens Roxb., and Zingiber zerumbet (L.) Smith were collected respectively from Nghe An, Province and Ha Tinh, Province,Vietnam. The root essential oils were obtained by water distillation and analyzed by Gas chromatography (GC) and Gas chromatography coupled with Mass spectrometry (GC-MS). The most abundant components of Z. rubens were (Z)-citral (30.1%), camphene (9.7%), β-phellandrene (7.5%) and 1,8-cineole (7.0%) and zingiberene (5.3%). The main oil constituents of Z. zerumbet were (Z)-citral (26.1%), camphene (16.3%), sabinene (14.6%), zingiberene (7.2%) and lavandulyl acetate (6.7%). This species has low zerumbone (1.2%) content.
基金Supported by the Applied Basic Research Project in Sichuan(2018)National Science and Technology Support Program(2015BAC05B02)Innovative Research Projects of Graduate Students in Southwest Minzu University in 2017(CX2017SZ085)
文摘[Objectives] Volatile oil components of traditional drug use site " root and rhizome" of N. jatamansi and herb were identified and contrasted,which aimed to provide the basis for rationally using wild N. jatamansi resource. [Methods]Volatile oil components from different sites of N. jatamansi were identified and isolated by GC-MS. [Results] There were 56 kinds of volatile oil components from different sites of N. jatamansi,in which 39 components from herb of N. jatamansi,39 components from root and rhizome of N. jatamansi,and there were 22 common components. But 1 component in higher concentration had obvious difference. [Conclusions] The herb of N. jatamansi could not completely replace " root and rhizome" of N. jatamansi as the medicine,which was consistent with prior detection result of each physicochemical index from different sites of N. jatamansi. The research could provide the reference for making quality standard of N. jatamansi medicine and rational basis for its further research and development.
