目的比较生物陶瓷材料iRoot BP Plus和无机三氧化物聚合物(MTA)形成根尖屏障修复根尖未发育完全恒牙的效果。方法回顾2018年1月至2022年8月医院牙体牙髓专家门诊收治的80例(80颗患牙)根尖未发育完全恒牙患者,按材料差异应用差异分为两...目的比较生物陶瓷材料iRoot BP Plus和无机三氧化物聚合物(MTA)形成根尖屏障修复根尖未发育完全恒牙的效果。方法回顾2018年1月至2022年8月医院牙体牙髓专家门诊收治的80例(80颗患牙)根尖未发育完全恒牙患者,按材料差异应用差异分为两组,A组40例:根尖屏障修复术中应用iRoot BP Plus材料;B组40例:术中应用MTA材料;对比两组修复效果。结果A组、B组效果评价比较(P>0.05)。A组充填操作时间(8.51±1.38)min,短于B组的(15.48±2.09)min,差异有统计学意义(P<0.05)。A组治疗6个月O-PAI评分低于B组,并发症发生率2.50%低于B组的20.00%(P<0.05)。结论iRoot BP Plus和MTA形成根尖屏障修复根尖未发育完全恒牙均有相似效果,但iRoot BP Plus充填时间短,并发症少,值得应用。展开更多
目的比较生物陶瓷根管修复剂(Iroot BP Plus)与氢氧化钙[Ca(OH)_(2)]在牙髓保存中的效果。方法选取2018年1月-2020年1月景德镇市第一人民医院收治的100例牙体牙髓疾病患者(100颗牙),其中因创伤所致前牙创伤露髓牙30例(30颗牙),因有冷热...目的比较生物陶瓷根管修复剂(Iroot BP Plus)与氢氧化钙[Ca(OH)_(2)]在牙髓保存中的效果。方法选取2018年1月-2020年1月景德镇市第一人民医院收治的100例牙体牙髓疾病患者(100颗牙),其中因创伤所致前牙创伤露髓牙30例(30颗牙),因有冷热刺激一过性疼痛深龋齿70例(70颗牙)。按随机数字表法将30颗前牙创伤露髓牙与70颗冷热刺激一过性疼痛深龋齿各分为2组,其中对照A组15颗,观察A组15颗,对照B组35颗,观察B组35颗。所有患者均予以活髓切断术,对照A组、对照B组术后以Ca(OH)_(2)填充治疗,观察A组、观察B组以Iroot BP Plus填充治疗,随访至术后3个月。对比4组临床疗效、牙髓保存成功率。结果观察A组、观察B组治疗1,3个月的治疗Ⅰ级有效率分别为80.00%(12/15)、93.33%(14/15)、71.43%(25/35)、91.43%(32/35),高于对照A组、对照B组的13.33%(2/15)、26.67%(4/15)、45.71%(16/35)、57.14%(20/35),差异有统计学意义(P<0.05);治疗3个月后,观察A组、观察B组牙髓保存成功率分别为100.00%(15/15)、97.14%(34/35),高于对照A组、对照B组的40.00%(6/15)65.71%(23/35),差异有统计学意义(P<0.05)。结论与Ca(OH)_(2),相比,Iroot BP Plus在牙髓保存中效果更为确切,可有效修复牙体缺损,提高牙髓保存成功率。展开更多
目的:探讨根管充填及修复材料(iRoot BP plus)和三氧化物凝聚体(MTA)根管修复材料用于牙髓血运重建术治疗年轻恒牙根尖感染的临床疗效。方法:选取医院口腔科收治的40例年轻恒牙根尖感染患者,按照随机数表法将其分为观察组和对照组,每组2...目的:探讨根管充填及修复材料(iRoot BP plus)和三氧化物凝聚体(MTA)根管修复材料用于牙髓血运重建术治疗年轻恒牙根尖感染的临床疗效。方法:选取医院口腔科收治的40例年轻恒牙根尖感染患者,按照随机数表法将其分为观察组和对照组,每组20例。所有患者均运用牙髓血运重建术进行治疗,其中观察组选用iRoot BP plus材料封闭根管上端,对照组选用MTA材料进行根管上端的封闭。比较两组患者的临床疗效、治疗周期、就诊次数、咀嚼恢复时间及根尖感染痊愈时间,比较两组患者的根管壁厚度、牙根长度以及患者对治疗的满意度。结果:牙髓血运重建术后,观察组治疗总有效率为95%,显著高于对照组的60%,差异有统计学意义(χ^(2)=8.326,P<0.05)。观察组与对照组相比,治疗周期、就诊次数、咀嚼恢复时间和根尖痊愈时间均显著缩短,差异具有统计学意义(t=12.492,t=10.424,t=6.524,t=11.907;P<0.05)。治疗后观察组患者的牙冠根长度显著长于对照组,根管壁厚度优于对照组,差异有统计学意义(t=8.742,t=7.048;P<0.05)。观察组患者咬合度、牙齿色泽、咀嚼能力及整体美观度4项满意度评分均显著高于对照组,差异有统计学意义(t=5.437,t=5.093,t=7.591,t=6.852;P<0.05)。结论:在运用牙髓血运重建术治疗年轻恒牙根尖感染时,iRoot BP plus材料较MTA材料更具有优势,可更有效提高患者的临床疗效和治疗满意度。展开更多
目的:探究纤维桩+复合树脂+氧化锆全瓷冠修复根管治疗后后牙楔状缺损患者的效果及美观满意度。方法:纳入2022年1月-2023年1月在笔者医院就诊的102例后牙楔状缺损患者,根据患者自主选择意愿分为研究组(n=50)和对照组(n=52),对照组行金属...目的:探究纤维桩+复合树脂+氧化锆全瓷冠修复根管治疗后后牙楔状缺损患者的效果及美观满意度。方法:纳入2022年1月-2023年1月在笔者医院就诊的102例后牙楔状缺损患者,根据患者自主选择意愿分为研究组(n=50)和对照组(n=52),对照组行金属桩核+金属烤瓷冠修复,研究组行纤维桩+复合树脂+氧化锆全瓷冠修复。6个月后,对比两组临床疗效,观察两组牙周健康情况(牙龈指数、菌斑指数、龈沟出血指数),检测牙周炎症[肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)、白细胞介素-4(Interleukin-4,IL-4)、白细胞介素-6(Interleukin-6,IL-6)],调查美观满意度[美国公共健康服务标准(United states public health service,USPHS)]和实用度(咀嚼功能、咬合情况、语言功能),比较两组并发症发生情况。结果:治疗后,研究组治疗有效率达92.00%,高于对照组的76.92%(P<0.05);两组牙周健康相关指数及TNF-α、IL-4、IL-6均较治疗前降低,且研究组降低幅度大于对照组(P<0.05);治疗后,研究组牙齿表面形态、色泽协调性、边缘着色满意度均高于对照组(P<0.05),两组咀嚼功能、咬合情况、语言功能均较治疗前提高,但差异无统计学意义(P>0.05);两组并发症发生率比较治疗组低于对照组(P<0.05)。结论:采用纤维桩+复合树脂+氧化锆全瓷冠修复根管治疗后后牙楔状缺损能够减轻炎症反应,提升患者牙周健康,增加美观满意度及实用度,疗效显著,安全性高。展开更多
Nerve conduits have been a viable alternative to the ‘gold standard’ autograft for treating small peripheral nerve gap injuries. However, they often produce inadequate functional recovery outcomes and are ineffectiv...Nerve conduits have been a viable alternative to the ‘gold standard’ autograft for treating small peripheral nerve gap injuries. However, they often produce inadequate functional recovery outcomes and are ineffective in large gap injuries. Ridge/groove surface micropatterning has been shown to promote neural cell orientation and guide growth. However, optimization of the ratio of ridge/groove parameters to promote orientation and extension for dorsal root ganglion （DRG） cells on poly（lactic-co-glycolic acid） （PLGA） films has not been previously conducted. Photolithography and micro-molding were used to define various combinations of ridge/groove dimensions on PLGA films. The DRG cells obtained from chicken embryos were cultured on micropatterned PLGA films for cell orientation and migration evaluation.Biodegradation of the films occurred during the test period, however, this did not cause deformation or distortion of the micropatterns. Results from the DRG cell orientation test suggest that when the ridge/groove ratio equals 1 （ridge/groove width parameters are equal, i.e., 10 μm/10 μm （even））, the degree of alignment depends on the size of the ridges and grooves, when the ratio is smaller than 1 （groove controlled） the alignment increases as the ridge size decreases, and when the ratio is larger than 1 （ridge controlled）, the alignment is reduced as the width of the grooves decreases. The migration rate and neurite extension of DRG neurons were greatest on 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films. Based on the data, the 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films are the optimized ridge/groove surface patterns for the construction of nerve repair devices.展开更多
Schwann cell transplantation is a promising method to promote neural repair, and can be used for peripheral nerve protection and myelination. Microcapsule technology largely mitigates immune rejection of transplanted ...Schwann cell transplantation is a promising method to promote neural repair, and can be used for peripheral nerve protection and myelination. Microcapsule technology largely mitigates immune rejection of transplanted cells. We previously showed that microencapsulated olfactory ensheathing cells can reduce neuropathic pain and we hypothesized that microencapsulated Schwann cells can also inhibit neuropathic pain. Rat Schwann cells were cultured by subculture and then microencapsulated and were tested using a rat chronic constriction injury（CCI） neuropathic pain model. CCI rats were treated with Schwann cells or microencapsulated Schwann cells and were compared with sham and CCI groups. Mechanical withdrawal threshold and thermal withdrawal latency were assessed preoperatively and at 1, 3, 5, 7, 9, 11 and 14 days postoperatively. The expression of P2X3 receptors in L4-5 dorsal root ganglia of the different groups was detected by double-label immunofluorescence on day 14 after surgery. Compared with the chronic constriction injury group, mechanical withdrawal threshold and thermal withdrawal latency were higher, but the expression of P2X3 receptors was remarkably decreased in rats treated with Schwann cells and microencapsulated Schwann cells, especially in the rats transplanted with microencapsulated Schwann cells. The above data show that microencapsulated Schwann cell transplantation inhibits P2X3 receptor expression in L4-5 dorsal root ganglia and neuropathic pain.展开更多
Direct coaptation of contralateral C7 to the upper trunk could avoid the interposition of nerve grafts. We have successfully shortened the gap and graft lengths, and even achieved direct coaptation. However, direct re...Direct coaptation of contralateral C7 to the upper trunk could avoid the interposition of nerve grafts. We have successfully shortened the gap and graft lengths, and even achieved direct coaptation. However, direct repair can only be performed in some selected cases, and partial procedures still require autografts, which are the gold standard for repairing neurologic defects. As symptoms often occur after autografting, human acellular nerve allografts have been used to avoid concomitant symptoms. This study investigated the quality of shoulder abduction and elbow flexion following direct repair and acellular allografting to evaluate issues requiring attention for brachial plexus injury repair. Fifty-one brachial plexus injury patients in the surgical database were eligible for this retrospective study. Patients were divided into two groups according to different surgical methods. Direct repair was performed in 27 patients, while acellular nerve allografts were used to bridge the gap between the contralateral C7 nerve root and upper trunk in 24 patients. The length of the harvested contralateral C7 nerve root was measured intraoperatively. Deltoid and biceps muscle strength, and degrees of shoulder abduction and elbow flexion were examined according to the British Medical Research Council scoring system;meaningful recovery was defined as M3–M5. Lengths of anterior and posterior divisions of the contralateral C7 in the direct repair group were 7.64 ± 0.69 mm and 7.55 ± 0.69 mm, respectively, and in the acellular nerve allografts group were 6.46 ± 0.58 mm and 6.43 ± 0.59 mm, respectively. After a minimum of 4-year follow-up, meaningful recoveries of deltoid and biceps muscles in the direct repair group were 88.89% and 85.19%, respectively, while they were 70.83% and 66.67% in the acellular nerve allografts group. Time to C5/C6 reinnervation was shorter in the direct repair group compared with the acellular nerve allografts group. Direct repair facilitated the restoration of shoulder abduction and elbow flexion. Thus, if direct coaptation is not possible, use of acellular nerve allografts is a suitable option. This study was approved by the Medical Ethical Committee of the First Affiliated Hospital of Sun Yat-sen University, China (Application ID:[2017] 290) on November 14, 2017.展开更多
文摘目的比较生物陶瓷材料iRoot BP Plus和无机三氧化物聚合物(MTA)形成根尖屏障修复根尖未发育完全恒牙的效果。方法回顾2018年1月至2022年8月医院牙体牙髓专家门诊收治的80例(80颗患牙)根尖未发育完全恒牙患者,按材料差异应用差异分为两组,A组40例:根尖屏障修复术中应用iRoot BP Plus材料;B组40例:术中应用MTA材料;对比两组修复效果。结果A组、B组效果评价比较(P>0.05)。A组充填操作时间(8.51±1.38)min,短于B组的(15.48±2.09)min,差异有统计学意义(P<0.05)。A组治疗6个月O-PAI评分低于B组,并发症发生率2.50%低于B组的20.00%(P<0.05)。结论iRoot BP Plus和MTA形成根尖屏障修复根尖未发育完全恒牙均有相似效果,但iRoot BP Plus充填时间短,并发症少,值得应用。
文摘目的比较生物陶瓷根管修复剂(Iroot BP Plus)与氢氧化钙[Ca(OH)_(2)]在牙髓保存中的效果。方法选取2018年1月-2020年1月景德镇市第一人民医院收治的100例牙体牙髓疾病患者(100颗牙),其中因创伤所致前牙创伤露髓牙30例(30颗牙),因有冷热刺激一过性疼痛深龋齿70例(70颗牙)。按随机数字表法将30颗前牙创伤露髓牙与70颗冷热刺激一过性疼痛深龋齿各分为2组,其中对照A组15颗,观察A组15颗,对照B组35颗,观察B组35颗。所有患者均予以活髓切断术,对照A组、对照B组术后以Ca(OH)_(2)填充治疗,观察A组、观察B组以Iroot BP Plus填充治疗,随访至术后3个月。对比4组临床疗效、牙髓保存成功率。结果观察A组、观察B组治疗1,3个月的治疗Ⅰ级有效率分别为80.00%(12/15)、93.33%(14/15)、71.43%(25/35)、91.43%(32/35),高于对照A组、对照B组的13.33%(2/15)、26.67%(4/15)、45.71%(16/35)、57.14%(20/35),差异有统计学意义(P<0.05);治疗3个月后,观察A组、观察B组牙髓保存成功率分别为100.00%(15/15)、97.14%(34/35),高于对照A组、对照B组的40.00%(6/15)65.71%(23/35),差异有统计学意义(P<0.05)。结论与Ca(OH)_(2),相比,Iroot BP Plus在牙髓保存中效果更为确切,可有效修复牙体缺损,提高牙髓保存成功率。
基金2022年度内蒙古自治区卫生健康科技计划(202202170)“iRoot BP plus和MTA用于牙髓血运重建术在治疗年轻恒牙根尖感染的临床疗效对比”。
文摘目的:探讨根管充填及修复材料(iRoot BP plus)和三氧化物凝聚体(MTA)根管修复材料用于牙髓血运重建术治疗年轻恒牙根尖感染的临床疗效。方法:选取医院口腔科收治的40例年轻恒牙根尖感染患者,按照随机数表法将其分为观察组和对照组,每组20例。所有患者均运用牙髓血运重建术进行治疗,其中观察组选用iRoot BP plus材料封闭根管上端,对照组选用MTA材料进行根管上端的封闭。比较两组患者的临床疗效、治疗周期、就诊次数、咀嚼恢复时间及根尖感染痊愈时间,比较两组患者的根管壁厚度、牙根长度以及患者对治疗的满意度。结果:牙髓血运重建术后,观察组治疗总有效率为95%,显著高于对照组的60%,差异有统计学意义(χ^(2)=8.326,P<0.05)。观察组与对照组相比,治疗周期、就诊次数、咀嚼恢复时间和根尖痊愈时间均显著缩短,差异具有统计学意义(t=12.492,t=10.424,t=6.524,t=11.907;P<0.05)。治疗后观察组患者的牙冠根长度显著长于对照组,根管壁厚度优于对照组,差异有统计学意义(t=8.742,t=7.048;P<0.05)。观察组患者咬合度、牙齿色泽、咀嚼能力及整体美观度4项满意度评分均显著高于对照组,差异有统计学意义(t=5.437,t=5.093,t=7.591,t=6.852;P<0.05)。结论:在运用牙髓血运重建术治疗年轻恒牙根尖感染时,iRoot BP plus材料较MTA材料更具有优势,可更有效提高患者的临床疗效和治疗满意度。
文摘目的:探究纤维桩+复合树脂+氧化锆全瓷冠修复根管治疗后后牙楔状缺损患者的效果及美观满意度。方法:纳入2022年1月-2023年1月在笔者医院就诊的102例后牙楔状缺损患者,根据患者自主选择意愿分为研究组(n=50)和对照组(n=52),对照组行金属桩核+金属烤瓷冠修复,研究组行纤维桩+复合树脂+氧化锆全瓷冠修复。6个月后,对比两组临床疗效,观察两组牙周健康情况(牙龈指数、菌斑指数、龈沟出血指数),检测牙周炎症[肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)、白细胞介素-4(Interleukin-4,IL-4)、白细胞介素-6(Interleukin-6,IL-6)],调查美观满意度[美国公共健康服务标准(United states public health service,USPHS)]和实用度(咀嚼功能、咬合情况、语言功能),比较两组并发症发生情况。结果:治疗后,研究组治疗有效率达92.00%,高于对照组的76.92%(P<0.05);两组牙周健康相关指数及TNF-α、IL-4、IL-6均较治疗前降低,且研究组降低幅度大于对照组(P<0.05);治疗后,研究组牙齿表面形态、色泽协调性、边缘着色满意度均高于对照组(P<0.05),两组咀嚼功能、咬合情况、语言功能均较治疗前提高,但差异无统计学意义(P>0.05);两组并发症发生率比较治疗组低于对照组(P<0.05)。结论:采用纤维桩+复合树脂+氧化锆全瓷冠修复根管治疗后后牙楔状缺损能够减轻炎症反应,提升患者牙周健康,增加美观满意度及实用度,疗效显著,安全性高。
文摘Nerve conduits have been a viable alternative to the ‘gold standard’ autograft for treating small peripheral nerve gap injuries. However, they often produce inadequate functional recovery outcomes and are ineffective in large gap injuries. Ridge/groove surface micropatterning has been shown to promote neural cell orientation and guide growth. However, optimization of the ratio of ridge/groove parameters to promote orientation and extension for dorsal root ganglion （DRG） cells on poly（lactic-co-glycolic acid） （PLGA） films has not been previously conducted. Photolithography and micro-molding were used to define various combinations of ridge/groove dimensions on PLGA films. The DRG cells obtained from chicken embryos were cultured on micropatterned PLGA films for cell orientation and migration evaluation.Biodegradation of the films occurred during the test period, however, this did not cause deformation or distortion of the micropatterns. Results from the DRG cell orientation test suggest that when the ridge/groove ratio equals 1 （ridge/groove width parameters are equal, i.e., 10 μm/10 μm （even））, the degree of alignment depends on the size of the ridges and grooves, when the ratio is smaller than 1 （groove controlled） the alignment increases as the ridge size decreases, and when the ratio is larger than 1 （ridge controlled）, the alignment is reduced as the width of the grooves decreases. The migration rate and neurite extension of DRG neurons were greatest on 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films. Based on the data, the 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films are the optimized ridge/groove surface patterns for the construction of nerve repair devices.
基金supported by the National Natural Science Foundation of China,No.81760418 and 81260190the Natural Science Foundation of Jiangxi Province,No.20132BAB205023,20151BAB205022+1 种基金a grant from Science and Technology Research Project of Jiangxi Education Department,No.GJJ13159a grant from the Science and Technology Program of Department of Health of Jiangxi Province,No.20173010
文摘Schwann cell transplantation is a promising method to promote neural repair, and can be used for peripheral nerve protection and myelination. Microcapsule technology largely mitigates immune rejection of transplanted cells. We previously showed that microencapsulated olfactory ensheathing cells can reduce neuropathic pain and we hypothesized that microencapsulated Schwann cells can also inhibit neuropathic pain. Rat Schwann cells were cultured by subculture and then microencapsulated and were tested using a rat chronic constriction injury（CCI） neuropathic pain model. CCI rats were treated with Schwann cells or microencapsulated Schwann cells and were compared with sham and CCI groups. Mechanical withdrawal threshold and thermal withdrawal latency were assessed preoperatively and at 1, 3, 5, 7, 9, 11 and 14 days postoperatively. The expression of P2X3 receptors in L4-5 dorsal root ganglia of the different groups was detected by double-label immunofluorescence on day 14 after surgery. Compared with the chronic constriction injury group, mechanical withdrawal threshold and thermal withdrawal latency were higher, but the expression of P2X3 receptors was remarkably decreased in rats treated with Schwann cells and microencapsulated Schwann cells, especially in the rats transplanted with microencapsulated Schwann cells. The above data show that microencapsulated Schwann cell transplantation inhibits P2X3 receptor expression in L4-5 dorsal root ganglia and neuropathic pain.
基金supported by the National Natural Science Foundation of China,No.81572130(to LQG)and 81601057(to JTY)the National Key Research and Development Plan of China,No.2016YFC1101603(to XLL)the Natural Science Foundation of Guangdong Province of China,No.2015A030310350(to JTY)
文摘Direct coaptation of contralateral C7 to the upper trunk could avoid the interposition of nerve grafts. We have successfully shortened the gap and graft lengths, and even achieved direct coaptation. However, direct repair can only be performed in some selected cases, and partial procedures still require autografts, which are the gold standard for repairing neurologic defects. As symptoms often occur after autografting, human acellular nerve allografts have been used to avoid concomitant symptoms. This study investigated the quality of shoulder abduction and elbow flexion following direct repair and acellular allografting to evaluate issues requiring attention for brachial plexus injury repair. Fifty-one brachial plexus injury patients in the surgical database were eligible for this retrospective study. Patients were divided into two groups according to different surgical methods. Direct repair was performed in 27 patients, while acellular nerve allografts were used to bridge the gap between the contralateral C7 nerve root and upper trunk in 24 patients. The length of the harvested contralateral C7 nerve root was measured intraoperatively. Deltoid and biceps muscle strength, and degrees of shoulder abduction and elbow flexion were examined according to the British Medical Research Council scoring system;meaningful recovery was defined as M3–M5. Lengths of anterior and posterior divisions of the contralateral C7 in the direct repair group were 7.64 ± 0.69 mm and 7.55 ± 0.69 mm, respectively, and in the acellular nerve allografts group were 6.46 ± 0.58 mm and 6.43 ± 0.59 mm, respectively. After a minimum of 4-year follow-up, meaningful recoveries of deltoid and biceps muscles in the direct repair group were 88.89% and 85.19%, respectively, while they were 70.83% and 66.67% in the acellular nerve allografts group. Time to C5/C6 reinnervation was shorter in the direct repair group compared with the acellular nerve allografts group. Direct repair facilitated the restoration of shoulder abduction and elbow flexion. Thus, if direct coaptation is not possible, use of acellular nerve allografts is a suitable option. This study was approved by the Medical Ethical Committee of the First Affiliated Hospital of Sun Yat-sen University, China (Application ID:[2017] 290) on November 14, 2017.
