基于模糊工具箱和ROSETTA的粗糙集数据挖掘

针对大量连续属性值的数据挖掘,提出了一种基于模糊工具箱和ROSETTA软件的粗糙集数据挖掘方法.在粗糙集理论的基础上,应用模糊工具箱中的模糊聚类方法离散分类连续属性值,并将其转化为粗糙集易于处理的知识表格.应用粗糙集数据挖掘软件ROSETTA对这些知识表格进行知识约简处理.通过约简知识属性和属性值,得到连续属性值的核心知识规则,并以实测数据为例,说明了该方法的实现过程和有效性.

粗集理论和Rosetta系统在教学质量评价中的应用

基于粗集理论,利用Rosetta系统对高校教学质量评价信息系统进行离散化和属性约简,得到了评价指标体系中的核心指标和决策规则。利用这些规则,测得被评估教师的实际教学水平,客观评价教师的教学质量,为教师教学质量评价提供一种新的分析方法。

一种利用噬菌体裂解大肠杆菌Rosetta菌株菌体的方法

Escherichia coli Rosetta菌株是基因克隆表达的重要宿主菌,为了探索裂解E.coli Rosetta菌体的新方法,从污水中分离到1株能裂解E.coli Rosetta菌株的噬菌体,命名为ETP-1,并研究其生物学特性和裂解效率。结果表明,EPT-1在双层平板上形成直径为1~3 mm的噬菌斑;噬菌斑透明,周围有晕环;该噬菌体的潜伏期为20min,裂解期为70 min,在55℃以上以及p H值大于11时,噬菌体失去活性。在6 h内,噬菌体可裂解73%的E.coli Rosetta菌体,ETP-1裂解其宿主细胞为E.coli Rosetta菌株菌体的破碎提供了新的方法。

基于Rosetta软件和Vague集的战术机动事件检测方法

事件检测是态势觉察的重要内容,是对当前战场态势变化的感知,是态势估计的基础,能否正确检测出当前战场所发生的有意义的行为,直接影响到了态势估计的结果。提出一种基于Rosetta软件和Vague集的战术机动事件检测方法,将目标历史数据离散化,建立事件决策表;利用Rosetta软件中的遗传算法约简方法对事件决策表进行约简,得到简约决策表;将实时检测到的目标状态变化离散化,根据简约决策表建立Vague集;计算事件的相似度,根据评价体系,判断发生何种事件。仿真结果表明,这种事件检测方法,较大地提高了检测的正确率。

Test of the Rosetta Pedotransfer Function for Saturated Hydraulic Conductivity

Simulation models are tools that can be used to explore, for example, effects of cultural practices on soil erosion and irrigation on crop yield. However, often these models require many soil related input data of which the saturated hydraulic conductivity (Ks) is one of the most important ones. These data are usually not available and experimental determination is both expensive and time consuming. Therefore, pedotransfer functions are often used, which make use of simple and often readily available soil information to calculate required input values for models, such as soil hydraulic values. Our objective was to test the Rosetta pedotransfer function to calculate Ks. Research was conducted in a 64-ha field near Lamesa, Texas, USA. Field measurements of soil texture and bulk density, and laboratory measurements of soil water retention at field capacity (–33 kPa) and permanent wilting point (–1500 kPa), were taken to implement Rosetta. Calculated values of Ks were then compared to measured Ks on undisturbed soil samples. Results showed that Rosetta could be used to obtain values of Ks for a field with different textures. The Root Mean Square Difference (RMSD) of Ks at 0.15 m soil depth was 7.81 × 10–7 m·s–1. Further, for a given soil texture the variability, from 2.30 × 10–7 to 2.66 × 10–6 m·s-1, of measured Ks was larger than the corresponding RMSD. We conclude that Rosetta is a tool that can be used to calculate Ks in the absence of measured values, for this particular soil. Level H5 of Rosetta yielded the best results when using the measured input data and thus calculated values of Ks can be used as input in simulation models.

Assessment the Physico-Chemical Characteristics of Water and Sediment in Rosetta Branch, Egypt

Water quality of Rosetta Branch may be changed by several factors in the last decades as a result of anthropogenic activities. So, it’s important to study the physicochemical characteristics of both water and sediment in the Rosetta Branch. Two identified sources are the main origin of most pollutants in this branch, namely: El-Rahawy drain and industrial activities in Kafr El-Zayat city. From the data of water quality index (WQI) based on six important parameters (pH, T °C, DO, BOD, COD and TP), it indicates that site 2 (from Kom Hamada to Edfina) is more polluted than the other two sites (from El-Qanater El-Khairia to Kom Hamada and from Edfina to Rosetta). The concentrations of heavy metals increase in sites that are more affected by drainage water from different drains. Great efforts are needed and wastewater must be treated before draining it into the River Nile water.

Pfu DNA聚合酶在大肠杆菌Rosetta(DE3)中的表达及快速纯化

来源于Pyrococcus furiosus COM1菌的Pfu DNA聚合酶具有保真性高、延伸速度快、热稳定性强等特点,能够提高PCR反应过程中的扩增效率,减少错误碱基掺入率.以大肠杆菌Rosetta(DE3)为重组细胞,利用一种快速表达纯化方法,让Pfu DNA聚合酶的表达纯化过程变得更加简便,并维持其高活性.结果表明,Pfu DNA聚合酶在大肠杆菌Rosetta(DE3)中能够进行大量表达,同时60℃高温水浴能有效去除杂蛋白,简化纯化步骤及提高Pfu DNA聚合酶纯度.经过一系列纯化步骤处理后,Pfu DNA聚合酶仍然能够保持较好的酶活,且在100μL PCR反应体系中,加入4μL的1 mg/mL Pfu DNA聚合酶液效果最佳.

Simulating the Effect of Improving Water Quality at the El-Rahawy and the Tala Drains in the Rosetta Branch Water Quality

The El-Rahawy and the Tala drains are the major sources of pollution along the Rosetta branch. The El-Rahawy drain receives primary treated wastewater from the Abu-Rawash Wastewater Treatment Plant (WWTP), while the Tala drain receives discharge from dairy industry and agricultural drainage, as well as domestic wastewater. This research involved attempting to study the effect of improving water quality on the El-Rahawy and the Tala drains in the Rosetta branch water quality. Water quality at the El-Rahawy drain is expected to improve after discharging water from the Al-Buhairi Water Canal to the El-Rahawy drain and improving effluent water quality at the Abu-Rawash WWTP. Water quality at the Tala drain is expected to improve after constructing a new WWTP and improving effluent water quality at the dairy industry. The river pollutant (RP) modeling enabled studying the effect of improving water quality at the drains on the Rosetta branch water quality. The RP modeling showed that applying the proposed solutions would significantly improve water quality at the Rosetta branch.

Improve Water Quality at the El-Rahawy Drain and the Rosetta Branch, Egypt

The El-Rahawy drain, is the major source of pollution along the Rosetta branch, receives primary treated wastewater from the Abu-Rawash Wastewater Treatment Plant (WWTP). The main purpose of this research was to manage water quality at the Rosetta branch by improving effluent water quality at the Abu-Rawash WWTP. This research involved attempting to determine the optimal dose of aluminum chloride (AlCl3) to reach an acceptable treatment at the Abu-Rawash WWTP. A dose of 2.0 mg of AlCl3 for each liter of wastewater was selected. Another approach involves discharging flow from Al-Buhairi Water Canal to the El-Rahawy drain in order to increase the dissolved oxygen (DO) concentration and reduce pollutant concentrations at the El-Rahawy drain. Applying these approaches will significantly improve water quality at the El-Rahawy drain. The river pollutant (RP) modeling was also used to study the effect of improving water quality at the El-Rahawy drain on the Rosetta branch water quality. The RP modeling showed that applying the proposed solutions will significantly improve water quality at the Rosetta branch.

Impact of Improving Water Quality at the Tala Drain on the Rosetta Branch Water Quality

The Tala drain is the second major source of pollution along the Rosetta branch. The Tala drain receives discharge from dairy industry and agricultural drainage, as well as untreated domestic wastewater. This research involved attempting to improve water quality at the Rosetta branch by improving water quality at the Tala drain. Water quality at the Tala drain will be improved through improving effluent water quality from the dairy industry using aluminum chloride (AlCl3) as a coagulant, with injections of carbon dioxide (CO2), and constructing a new WWTP. Results indicated that the optimum aluminum chloride dosage was 225 mg/L at a pH value of 6.15. The estimated treatment cost of 1.0 m3 of dairy wastewater is $0.0425 per day. The river pollutant (RP) modeling was also used to study the effect of improving water quality at the Tala drain in the Rosetta branch water quality. The RP modeling showed that applying the proposed solutions will significantly improve water quality at the Tala drain and at the Rosetta branch.

供应链管理与Rosetta Net标准

1供应链管理 当今全球已步人经济一体化和因特网新经济时代,市场的变化正在不断加快,需求的不确定性正在不断加大,势必要求信息交换快捷与准确,产品上市快速与及时.原来传统的企业管理模式--"纵向一体化"管理已不适应新时代的要求.

“Rosetta stone”的启示

笔者译《国际晶体生长协会第五次大会程序记录》,内有大会主席劳迪斯的序言(Proceeding of the Fifth International Conference on Crystal Growth,Preface,by Co-chairman Laudise),序言中有这样一句话:

改善稀有密码子和氨基酸残基限制提高重组人ADAM15去整合素结构域蛋白表达水平

【目的】为提高重组人ADAM(A Disintegrin And Metalloproteinase)15去整合素结构域蛋白(rhADAM15)的表达水平。【方法】在详尽分析rhADAM15的cDNA和GST(谷胱甘肽-S-转移酶)-ADAM15结构的基础上,选择表达宿主菌并对表达质粒进行改造。【结果】(1)选择能为大肠杆菌稀有密码子提供额外tRNA的Escherichia coli.Rosetta(DE3)作为宿主菌,将质粒pGEX-ADAM15转化于其中在最佳诱导表达条件下获得298mg/L融合蛋白GST-ADAM15;(2)采用PCR体外定点突变技术将目标蛋白编码区稀有密码子GGA(Gly425)替换为GGC,使融合蛋白表达水平提高9.4%;(3)通过消除凝血酶识别序列附近的Pro-Glu-Phe残基,提高凝血酶酶切效率,使rhADAM15产量提高了35.7%;(4)在GGA替换为GGC基础上切除"Pro-Glu-Phe"残基,使rhADAM15产量提高到68mg/L,比分别切除"Pro-Glu-Phe"残基、GGA替换为GGC和野生型提高了19.2%、51.1%和61.9%。【结论】这一结果表明,在充分认识目标蛋白特性的基础上定向选择表达宿主并改造表达质粒能实现外源蛋白高水平表达。

基于粗集理论的中医喘证临床医案关联规则分析

中医临床经验和医案的丰富和庞杂,迫切需要高效的医案分析方法进行辅助学习,通过现代数据处理技术对中医用药经验进行量化研究,为中医走向现代化探索一条有效的研究途径。ROSETTA系统是目前最完整灵活、先进的粗糙集软件系统,基于分类规则,为医学临床诊断提供了科学的数据挖掘和分析方法。该文以中医喘证临床医案为例,详细论述了应用该系统处理症状信息和挖掘用药配伍规律的全过程,为中医临床经验的传承和学习提供了新的研究思路。

一种基于片段组装的蛋白质构象空间优化算法

针对蛋白质构象空间优化问题,提出一种基于片段组装的构象空间优化算法。算法利用基于Rosetta粗粒度的知识能量模型有效地提高了收敛速度;同时,借助片段组装技术可以有效弥补因能量函数不精确而导致的预测精度不足的缺陷;此外,差分进化算法的引入使得算法具有较好的全局搜索能力。5种测试蛋白的实验结果表明,所提算法具有较好的搜索性能和预测精度。

鸡骨钙素蛋白的原核表达及其生物学活性

为获得具有生物活性的骨钙素蛋白,以鸡颅骨提取的总RNA为模板,RT-PCR法克隆鸡骨钙素成熟肽DNA,连接至原核表达载体pET-32a(+),构建表达质粒pET-32a(+)-rchOC。质粒转化Rosetta-gami(DE3)pLyS宿主菌,IPTG诱导表达含His标签的重组蛋白His-rchOC。肠激酶酶切去除融合蛋白中的His标签后获得重组骨钙素蛋白(rchOC)。通过与羟基磷灰石结合试验、MTT及PNPP法分析该蛋白生物学活性。结果显示:重组骨钙素蛋白能与羟基磷灰石结合,抑制成骨细胞中碱性磷酸酶的表达,但不影响成骨细胞的增殖。结论:经原核表达系统已成功获得鸡重组骨钙素蛋白,且该蛋白具有生物活性。

基于粗糙集理论的数据挖掘的应用

在粗糙集理论的基本思想及其特点的基础上说明它是数据挖掘的一种工具 ,并对其广泛的应用作了描述。介绍了常见的粗糙集理论的应用工具软件 (如 Rosetta) ,并给出了一个应用粗糙集技术来实现数据挖掘的实例系统 (电力系统中操作点的分类 )

基于粗糙集理论的原发性高血压肝阳上亢证辨证系统的建立

从与原发性高血压相关的古今医案中收集病例237例,对其症状和体征进行语言规范化处理及量化赋值,使用基于粗糙集理论的KDD决策分析系统Rosetta软件约简病证属性,获取原发性高血压肝阳上亢证的专家知识,并提供该系统决策规则的统计信息,为进一步研究原发性高血压肝阳上亢证奠定基础。

两个大豆MYB转录因子在原核细胞中的高效表达

大豆(Glycine max)GmMYB12a与GmMYB12B2基因属于典型的植物R2R3-MYB转录因子。为进一步研究两个MYB12转录因子与相应顺式作用元件的相互作用,构建了两基因的原核表达载体,并在大肠杆菌Rosetta(DE3)中实现高效表达。利用PCR技术,将两端带有特异酶切位点的GmMYB12a与GmMYB12B2全长基因亚克隆于原核表达载体pET-28a(+),酶切、测序鉴定确认获得两基因的重组原核表达载体pET-28a-GmMYB12a与pET-28a-GmMYB12B2。然后把重组载体转化到大肠杆菌Rosetta(DE3)中,经IPTG诱导蛋白表达,提取细胞蛋白并采用SDS-PAGE检测目的蛋白的表达情况。结果表明,成功构建了两基因的原核表达载体,在IPTG浓度为1.2 mmol/L,诱导6 h后,目的蛋白能在Rosetta(DE3)中高效表达。

科技与金融结合效益评价及最优金融结构探索——以广东省为例

文章针对广东省各市科技金融绩效问题,采用数据包络分析(DEA)方法进行绩效评价研究;接着根据效益评价结果,探索最优金融投入结构。结果表明:广东省部分城市存在金融投入规模不当、金融投入渠道简单、金融投入资源的管理效率低;科技与金融结合效率低的主要原因是不合理的金融投入结构。