目的采用双定性双定量相似度作为评价指标,建立了复方丹参滴丸(CDDP)中低波长紫外吸收指纹成分的控制方法。方法采用RP-HPLC法以Century SIL C18BDS柱(200 mm×4.6 mm,5μm);以色谱指纹图谱指数F为目标函数优化选择指纹图谱检测条件...目的采用双定性双定量相似度作为评价指标,建立了复方丹参滴丸(CDDP)中低波长紫外吸收指纹成分的控制方法。方法采用RP-HPLC法以Century SIL C18BDS柱(200 mm×4.6 mm,5μm);以色谱指纹图谱指数F为目标函数优化选择指纹图谱检测条件,确定流动相为水-乙腈低压梯度洗脱,紫外检测波长:203 nm,柱温:(30.00±0.15)℃,进样量:10μL。以双定性双定量相似度法对10批CDDP进行质量评价。结果以人参皂苷Rg1为参照物峰,确定18个共有指纹峰,建立了CDDP低波长紫外吸收指纹成分的HPLC指纹图谱。评价出10批CDDP的双定性双定量相似度均合格,表明此10批样品的低波长紫外吸收指纹成分的数量、分布比例和含量特征是十分相似的,表现出很好的质量均一性。结论本试验证明双定性双定量相似度法可有效控制CDDP中低波长紫外吸收指纹成分。展开更多
The effects of Acidithiobacillus ferrooxidans(At. f) mutated with diethyl sulfate(DES) as a mutagen on the bioleaching of soluble phosphorus(P) from rock phosphate(RP) were investigated. The results show that the oxid...The effects of Acidithiobacillus ferrooxidans(At. f) mutated with diethyl sulfate(DES) as a mutagen on the bioleaching of soluble phosphorus(P) from rock phosphate(RP) were investigated. The results show that the oxidative activity of At. f is greatly improved by 1.0%(volume fraction) of DES. Correspondingly,the highest leaching rate of soluble P is also obtained to be 14.9% by the At. f mutated,which is 85.8% higher than that of the adapted At. f without mutation. In addition,the SEM images are significantly performed that the corrosion of RP residue surfaces leached by 1.0% DES-induced At.f is much worse than that of leached by the adapted At. f. All the above indicate that the leaching efficiency of soluble P from RP with pyrite can be greatly improved by using DES-induced At. f to a certain extent.展开更多
目的:测定并比较不同产地蒙药材大栀子中栀子苷的含量,为大栀子的质量控制提供依据。方法:采用高效液相色谱法,色谱柱:C-18柱,流动相为:乙腈-水(15∶85),流速1.0 m L/min,检测波长238nm。结果:10个不同产地的蒙药材大栀子中栀子...目的:测定并比较不同产地蒙药材大栀子中栀子苷的含量,为大栀子的质量控制提供依据。方法:采用高效液相色谱法,色谱柱:C-18柱,流动相为:乙腈-水(15∶85),流速1.0 m L/min,检测波长238nm。结果:10个不同产地的蒙药材大栀子中栀子苷含量在4.6%-8.6%,其中贵州所产的一个批次中栀子苷含量最高(8.6%)。结论:不同产地及不同采收时间的蒙药材大栀子中栀子苷的含量差异显著。展开更多
基金Project (Z200515002) supported by the Key Project Foundation of the Education Department of Hubei Province, China
文摘The effects of Acidithiobacillus ferrooxidans(At. f) mutated with diethyl sulfate(DES) as a mutagen on the bioleaching of soluble phosphorus(P) from rock phosphate(RP) were investigated. The results show that the oxidative activity of At. f is greatly improved by 1.0%(volume fraction) of DES. Correspondingly,the highest leaching rate of soluble P is also obtained to be 14.9% by the At. f mutated,which is 85.8% higher than that of the adapted At. f without mutation. In addition,the SEM images are significantly performed that the corrosion of RP residue surfaces leached by 1.0% DES-induced At.f is much worse than that of leached by the adapted At. f. All the above indicate that the leaching efficiency of soluble P from RP with pyrite can be greatly improved by using DES-induced At. f to a certain extent.
文摘目的:测定并比较不同产地蒙药材大栀子中栀子苷的含量,为大栀子的质量控制提供依据。方法:采用高效液相色谱法,色谱柱:C-18柱,流动相为:乙腈-水(15∶85),流速1.0 m L/min,检测波长238nm。结果:10个不同产地的蒙药材大栀子中栀子苷含量在4.6%-8.6%,其中贵州所产的一个批次中栀子苷含量最高(8.6%)。结论:不同产地及不同采收时间的蒙药材大栀子中栀子苷的含量差异显著。