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无人机辅助物联网中基于Safe Actor-Critic的信息年龄最小化研究
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作者 魏宪鹏 付芳 张志才 《测试技术学报》 2024年第1期71-78,共8页
无人机作为一种新的通信设备,有望在物联网数据采集、监控等业务中发挥关键作用。为保证所采集数据的时效性,利用信息年龄来衡量无人机从物联网设备接收到的数据新鲜度。通过联合优化无人机轨迹和无人机与物联网设备的关联策略以最小化... 无人机作为一种新的通信设备,有望在物联网数据采集、监控等业务中发挥关键作用。为保证所采集数据的时效性,利用信息年龄来衡量无人机从物联网设备接收到的数据新鲜度。通过联合优化无人机轨迹和无人机与物联网设备的关联策略以最小化信息年龄加权和,并保证无人机累积飞行能量消耗满足预算要求。由于上述问题同时受短期和长期约束条件的限制,将问题建模为受约束的马尔可夫决策过程(CMDP),并利用Safe Actor-Critic来求解。仿真结果表明,所提算法在最小化信息年龄的同时,能有效保证能量预算。 展开更多
关键词 无人机 信息年龄 物联网 safe Actor-Critic
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A cross-sectional study to assess medication safety,knowledge,attitude,and practices regarding nutrition and medication among pregnant women
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作者 Gauthami R Bipin Shaji +3 位作者 Twinkle MJS Krishnapriya Radhakrishnan Reshma Kolar Juno Jerold Joel 《Asian pacific Journal of Reproduction》 CAS 2024年第3期115-119,共5页
Objective:To assess pregnant women's knowledge,attitude,and practice regarding nutrition and medication usage,analyse the prescribing pattern,and categorize them based on the Food and Drug Administration(FDA)guide... Objective:To assess pregnant women's knowledge,attitude,and practice regarding nutrition and medication usage,analyse the prescribing pattern,and categorize them based on the Food and Drug Administration(FDA)guidelines.Methods:A cross-sectional study was conducted with 264 pregnant women in the obstetrics and gynaecology department of a tertiary care hospital from October 2022 to August 2023.A knowledge,attitude,and practice(KAP)questionnaire was prepared in English language by the researchers and validated by an expert panel consisting of 12 members.The validated questionnaire was then translated into regional languages,Kannada and Malayalam.The reliability of the questionnaire was assessed with test-retest method with a representative sample population of 30 subjects(10 subjects for each language).The subjects'knowledge,attitude,and practice were evaluated using the validated KAP questionnaire.The safety of the medication was assessed using the FDA drug safety classification for pregnancy.Results:The mean scores for nutritional and medication usage knowledge,attitude,and practice were 4.14±1.15,4.50±1.09,and 3.00±1.47,respectively.Among 30 prescribed medications,3 belong to category A(no risk in human studies),8 belong to category B(no risk in animal studies),18 belong to category C(risk cannot be ruled out)and 1 drug is not classified.A significant association was observed between medication knowledge and practice(r=0.159,P=0.010).Conclusions:Most of the study population knows the need to maintain good dietary and medication practices during pregnancy.Counselling pregnant women regarding diet and medication usage is crucial in maternal care. 展开更多
关键词 PREGNANCY NUTRITION MEDICATION KNOWLEDGE Practice safe medication
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A Game-Theoretic Approach to Safe Crowd Evacuation in Emergencies
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作者 Maria Gul Imran Ali Khan +9 位作者 Gohar Zaman Atta Rahman Jamaluddin Mir Sardar Asad Ali Biabani May IssaAldossary Mustafa Youldash Ashraf Saadeldeen Maqsood Mahmud Asiya Abdus Salam Dania Alkhulaifi 《Computers, Materials & Continua》 SCIE EI 2024年第4期1631-1657,共27页
Obstacle removal in crowd evacuation is critical to safety and the evacuation system efficiency. Recently, manyresearchers proposed game theoreticmodels to avoid and remove obstacles for crowd evacuation. Game theoret... Obstacle removal in crowd evacuation is critical to safety and the evacuation system efficiency. Recently, manyresearchers proposed game theoreticmodels to avoid and remove obstacles for crowd evacuation. Game theoreticalmodels aim to study and analyze the strategic behaviors of individuals within a crowd and their interactionsduring the evacuation. Game theoretical models have some limitations in the context of crowd evacuation. Thesemodels consider a group of individuals as homogeneous objects with the same goals, involve complex mathematicalformulation, and cannot model real-world scenarios such as panic, environmental information, crowds that movedynamically, etc. The proposed work presents a game theoretic model integrating an agent-based model to removethe obstacles from exits. The proposed model considered the parameters named: (1) obstacle size, length, andwidth, (2) removal time, (3) evacuation time, (4) crowd density, (5) obstacle identification, and (6) route selection.The proposed work conducts various experiments considering different conditions, such as obstacle types, obstacleremoval, and several obstacles. Evaluation results show the proposed model’s effectiveness compared with existingliterature in reducing the overall evacuation time, cell selection, and obstacle removal. The study is potentially usefulfor public safety situations such as emergency evacuations during disasters and calamities. 展开更多
关键词 safe crowd evacuation public safety EMERGENCY transition probability COOPERATION
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Risk-Informed Model-Free Safe Control of Linear Parameter-Varying Systems
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作者 Babak Esmaeili Hamidreza Modares 《IEEE/CAA Journal of Automatica Sinica》 SCIE EI CSCD 2024年第9期1918-1932,共15页
This paper presents a risk-informed data-driven safe control design approach for a class of stochastic uncertain nonlinear discrete-time systems.The nonlinear system is modeled using linear parameter-varying(LPV)syste... This paper presents a risk-informed data-driven safe control design approach for a class of stochastic uncertain nonlinear discrete-time systems.The nonlinear system is modeled using linear parameter-varying(LPV)systems.A model-based probabilistic safe controller is first designed to guarantee probabilisticλ-contractivity(i.e.,stability and invariance)of the LPV system with respect to a given polyhedral safe set.To obviate the requirement of knowing the LPV system model and to bypass identifying its open-loop model,its closed-loop data-based representation is provided in terms of state and scheduling data as well as a decision variable.It is shown that the variance of the closedloop system,as well as the probability of safety satisfaction,depends on the decision variable and the noise covariance.A minimum-variance direct data-driven gain-scheduling safe control design approach is presented next by designing the decision variable such that all possible closed-loop system realizations satisfy safety with the highest confidence level.This minimum-variance approach is a control-oriented learning method since it minimizes the variance of the state of the closed-loop system with respect to the safe set,and thus minimizes the risk of safety violation.Unlike the certainty-equivalent approach that results in a risk-neutral control design,the minimum-variance method leads to a risk-averse control design.It is shown that the presented direct risk-averse learning approach requires weaker data richness conditions than existing indirect learning methods based on system identification and can lead to a lower risk of safety violation.Two simulation examples along with an experimental validation on an autonomous vehicle are provided to show the effectiveness of the presented approach. 展开更多
关键词 Data-driven control linear parameter-varying systems probabilistic control safe control
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Enhanced Memory-Safe Linux Security Modules (eLSMs) for Improving Security of Docker Containers for Data Centers
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作者 Juan Martinez Delbugio Vijay K. Madisetti 《Journal of Software Engineering and Applications》 2024年第5期259-269,共11页
The adoption of Docker containers has revolutionized software deployment by providing a lightweight and efficient way to isolate applications in data centers. However, securing these containers, especially when handli... The adoption of Docker containers has revolutionized software deployment by providing a lightweight and efficient way to isolate applications in data centers. However, securing these containers, especially when handling sensitive data, poses significant challenges. Traditional Linux Security Modules (LSMs) such as SELinux and AppArmor have limitations in providing fine-grained access control to files within containers. This paper presents a novel approach using eBPF (extended Berkeley Packet Filter) to implement a LSM that focuses on file-oriented access control within Docker containers. The module allows the specification of policies that determine which programs can access sensitive files, providing enhanced security without relying solely on the host operating system’s major LSM. 展开更多
关键词 DOCKER LSM MAC RUST Memory safe Languages
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A Dual-Task Learning Approach for Bearing Anomaly Detection and State Evaluation of Safe Region
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作者 Yuhua Yin Zhiliang Liu +1 位作者 Bin Guo Mingjian Zuo 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2024年第1期229-241,共13页
Predictive maintenance has emerged as an effective tool for curbing maintenance costs,yet prevailing research predominantly concentrates on the abnormal phases.Within the ostensibly stable healthy phase,the reliance o... Predictive maintenance has emerged as an effective tool for curbing maintenance costs,yet prevailing research predominantly concentrates on the abnormal phases.Within the ostensibly stable healthy phase,the reliance on anomaly detection to preempt equipment malfunctions faces the challenge of sudden anomaly discernment.To address this challenge,this paper proposes a dual-task learning approach for bearing anomaly detection and state evaluation of safe regions.The proposed method transforms the execution of the two tasks into an optimization issue of the hypersphere center.By leveraging the monotonicity and distinguishability pertinent to the tasks as the foundation for optimization,it reconstructs the SVDD model to ensure equilibrium in the model’s performance across the two tasks.Subsequent experiments verify the proposed method’s effectiveness,which is interpreted from the perspectives of parameter adjustment and enveloping trade-offs.In the meantime,experimental results also show two deficiencies in anomaly detection accuracy and state evaluation metrics.Their theoretical analysis inspires us to focus on feature extraction and data collection to achieve improvements.The proposed method lays the foundation for realizing predictive maintenance in a healthy stage by improving condition awareness in safe regions. 展开更多
关键词 Bearing condition monitoring Anomaly detection safe region Support vector data description
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Study on the Safety and Prevention Technology of Coal Mining under the River in Xingyuan Coal Mine
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作者 Abdoulaye Sylla Wenbing Guo 《Open Journal of Geology》 CAS 2024年第3期339-402,共64页
Coal mining-induced surface subsidence poses significant ecological and infrastructural challenges, necessitating a comprehensive study to ensure safe mining practices, particularly in underwater conditions. This proj... Coal mining-induced surface subsidence poses significant ecological and infrastructural challenges, necessitating a comprehensive study to ensure safe mining practices, particularly in underwater conditions. This project aims to address the extensive impact of coal mining on the environment, infrastructure, and overall safety, focusing on the Shigong River area above the working face. The study employs qualitative and quantitative analyses, along with on-site engineering measurements, to gather data on crucial parameters such as coal seam characteristics, roof rock lithology, thickness, water resistance, and structural damage degree. The research encompasses a multidisciplinary approach, involving mining, geology, hydrogeology, geophysical exploration, rock mechanics, mine surveying, and computational mathematics. The importance of effective safety measures and prevention techniques is emphasized, laying the foundation for research focused on the Xingyun coal mine. The brief concludes by highlighting the potential economic and social benefits of this project and its contribution to valuable experience for future subsea coal mining. 展开更多
关键词 Coal Mining-Induced Surface Subsidence Ecological and Infrastructural Challenges safe Mining Practices Underwater Conditions Multidisciplinary Approach safety Measures Prevention Techniques
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Smart materials for safe lithium-ion batteries against thermal runaway
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作者 Yu Ou Pan Zhou +5 位作者 Wenhui Hou Xiao Ma Xuan Song Shuaishuai Yan Yang Lu Kai Liu 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2024年第7期360-392,共33页
In recent years,the new energy storage system,such as lithium ion batteries(LIBs),has attracted much attention.In order to meet the demand of industrial progress for longer cycle life,higher energy density and cost ef... In recent years,the new energy storage system,such as lithium ion batteries(LIBs),has attracted much attention.In order to meet the demand of industrial progress for longer cycle life,higher energy density and cost efficiency,a quantity of research has been conducted on the commercial application of LIBs.However,it is difficult to achieve satisfying safety and cycling performance simultaneously.There may be thermal runaway(TR),external impact,overcharge and overdischarge in the process of battery abuse,which makes the safety problem of LIBs more prominent.In this review,we summarize recent progress in the smart safety materials design towards the goal of preventing TR of LIBs reversibly from different abuse conditions.Benefiting from smart responsive materials and novel structural design,the safety of LIBs can be improved a lot.We expect to provide a comprehensive reference for the development of smart and safe lithium-based battery materials. 展开更多
关键词 Lithium ion batteries(LIBs) Thermal runaway(TR) Smart materials safe batteries Solid electrolyte interface(SEI)
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Application and Effect Evaluation of the Integrated“5A and 3+3”Management Model in the Safe Medication Use for Chemotherapy Patients
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作者 Li He Tingting Huo +3 位作者 Na Yao Yi Liu Yingdi Wei Yan Zhang 《Journal of Clinical and Nursing Research》 2024年第9期14-23,共10页
Objective:To explore the application and effect evaluation of the integrated“5A and 3+3”management model in ensuring safe medication use for chemotherapy patients.Methods:A total of 100 intravenous chemotherapy pati... Objective:To explore the application and effect evaluation of the integrated“5A and 3+3”management model in ensuring safe medication use for chemotherapy patients.Methods:A total of 100 intravenous chemotherapy patients admitted to the oncology department of Shaanxi Provincial People’s Hospital were randomly divided into two groups using a random number list method.Both groups received conventional nursing management during chemotherapy,while the study group additionally received the integrated“5A and 3+3”safety management model.The nursing intervention effects between the two groups were compared.Results:After the intervention,the study group showed higher levels of self-management ability,compliance,and nursing satisfaction compared to the control group.The overall incidence of adverse events during hospitalization was lower in the study group,with statistically significant differences(P<0.05).The knowledge scores of medical staff in the study group,related to the prevention and treatment of chemotherapy drug side effects,daily symptom management,and daily life management,were higher than those in the control group,with statistically significant differences(P<0.05).Conclusion:Implementing the integrated“5A and 3+3”model in the safe medication management of intravenous chemotherapy patients can effectively enhance patients’self-management abilities and compliance,improve medical staff’s ability to safely administer chemotherapy drugs,reduce adverse events caused by chemotherapy,and increase patient satisfaction. 展开更多
关键词 Intravenous chemotherapy safe medication Adverse drug reactions “5A”management “3+3”management
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Tomographic velocity inversion for ADCIGs in areas with a rugged surface 被引量:2
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作者 张凯 李振春 +2 位作者 曾同生 秦宁 姚云霞 《Applied Geophysics》 SCIE CSCD 2012年第3期313-318,361,共7页
Pre-stack depth migration velocity analysis is one of the key techniques influencing image quality. As for areas with a rugged surface and complex subsurface, conventional prestack depth migration velocity analysis co... Pre-stack depth migration velocity analysis is one of the key techniques influencing image quality. As for areas with a rugged surface and complex subsurface, conventional prestack depth migration velocity analysis corrects the rugged surface to a known datum or designed surface velocity model on which to perform migration and update the velocity. We propose a rugged surface tomographic velocity inversion method based on angle-domain common image gathers by which the velocity field can be updated directly from the rugged surface without static correction for pre-stack data and improve inversion precision and efficiency. First, we introduce a method to acquire angle-domain common image gathers (ADCIGs) in rugged surface areas and then perform rugged surface tornographic velocity inversion. Tests with model and field data prove the method to be correct and effective. 展开更多
关键词 rugged surface angle-domain common image gathers tomographic inversion velocity modeling
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Two-dimensional frequency-domain acoustic fullwaveform inversion with rugged topography 被引量:1
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作者 张钱江 戴世坤 +3 位作者 陈龙伟 李昆 赵东东 黄兴兴 《Applied Geophysics》 SCIE CSCD 2015年第3期378-388,467,468,共13页
We studied finite-element-method-based two-dimensional frequency-domain acoustic FWI under rugged topography conditions. The exponential attenuation boundary condition suitable for rugged topography is proposed to sol... We studied finite-element-method-based two-dimensional frequency-domain acoustic FWI under rugged topography conditions. The exponential attenuation boundary condition suitable for rugged topography is proposed to solve the cutoff botmdary problem as well as to consider the requirement of using the same subdivision grid in joint multifrequency inversion. The proposed method introduces the attenuation factor, and by adjusting it, acoustic waves are sufficiently attenuated in the attenuation layer to minimize the cutoff boundary effect. Based on the law of exponential attenuation, expressions for computing the attenuation factor and the thickness of attenuation layers are derived for different frequencies. In multifrequency-domain FWI, the conjugate gradient method is used to solve equations in the Gauss-Newton algorithm and thus minimize the computation cost in calculating the Hessian matrix. In addition, the effect of initial model selection and frequency combination on FWI is analyzed. Examples using numerical simulations and FWI calculations are used to verify the efficiency of the proposed method. 展开更多
关键词 Full-waveform inversion rugged topography attenuation boundary condition finite element method
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Rugged Resonant Pole and Its Applications in Soft-Switching Converter
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作者 肖岚 严仰光 《Journal of Southeast University(English Edition)》 EI CAS 2002年第3期225-232,共8页
The soft switching operation principle and operation performance of rugged resonant pole (RRP) is given. The applications of RRP in soft switching DC DC converter and soft switching inverter are discussed in detail. R... The soft switching operation principle and operation performance of rugged resonant pole (RRP) is given. The applications of RRP in soft switching DC DC converter and soft switching inverter are discussed in detail. RRP can constitute buck boost soft switching DC DC converter and isolated soft switching DC DC converter with the automatic limitation performance of output power. Partial series resonant DC DC converter with RRP can realize the zero voltage/zero current switching of power devices. RR... 展开更多
关键词 rugged resonant pole soft switching DC DC converter INVERTER
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Distributed modeling of direct solar radiation on rugged terrain of the Yellow River Basin 被引量:4
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作者 ZENG Yan QIU Xinfa +1 位作者 LIU Changming JIANG Aijun 《Journal of Geographical Sciences》 SCIE CSCD 2005年第4期439-447,共9页
Due to the influences of local topographical factors and terrain inter-shielding, calculation of direct solar radiation (DSR) quantity of rugged terrain is very complex. Based on digital elevation model (DEM) data... Due to the influences of local topographical factors and terrain inter-shielding, calculation of direct solar radiation (DSR) quantity of rugged terrain is very complex. Based on digital elevation model (DEM) data and meteorological observations, a distributed model for calculating DSR over rugged terrain is developed. This model gives an all-sided consideration on factors influencing th a resolution of 1 km × 1 km for thDSR. Using the developed model, normals of annual DSR quantity wie Yellow River Basin was generated, with DEM data as the general characterization of terrain. Characteristics of DSR quantity influenced by geographic and topographic factors over rugged terrain were analyzed thoroughly. Results suggest that: influenced by local topographic factors, i.e. azimuth, slope and so on, and annual DSR quantity over mountainous area has a clear spatial difference; annual DSR quantity of sunny slope (or southern slope) of mountains is obviously larger than that of shady slope (or northern slope). The calculated DSR quantity of the Yellow River Basin is provided in the same way as other kinds of spatial information and can be employed as basic geographic data for relevant studies as well. 展开更多
关键词 direct solar radiation (DSR) rugged terrain digital elevation model (DEM) distributed model Yellow River Basin
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Distributed Modeling of Extraterrestrial Solar Radiation Over the Rugged Terrains of Pakistan 被引量:3
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作者 Romana AMBREEN QIU Xinfa Iftikhar AHMAD 《Journal of Mountain Science》 SCIE CSCD 2011年第3期427-436,共10页
In Pakistan,the solar analogue has been addressed but its surface geographical parameterization has given least attention.Inappropriate density of stations and their spatial coverage particularly in difficult peripher... In Pakistan,the solar analogue has been addressed but its surface geographical parameterization has given least attention.Inappropriate density of stations and their spatial coverage particularly in difficult peripheral national territories,little or no solar radiation data,non-satisfactory sunshine hours data,and low quality of ground observed cloud cover data create a situation in which the spatial modeling of Extraterrestrial Solar Radiation(ESR) and its ground parameterization got sufficient scope.The Digital Elevation Model (DEM) input into Geographic Information System (GIS) is a compatible tool to demonstrate the spatial distribution of ESR over the rugged terrains of the study domain.For the first time,distributed modeling of ESR is done over the rugged terrains of Pakistan,based on DEM and ArcGIS..Results clearly depict that the complex landforms profoundly disrupt the zonal distribution of ESR in Pakistan.The screening impact of topography is higher on spatial distribution of ESR in winter and considerably low in summer.The combined effect of topography and latitude is obvious.Hence,the model was further testified by plotting Rb (ratio of ESR quantity over rugged terrain against plane surface) against azimuth at different latitudes with different angled slopes.The results clearly support the strong screening effect of rugged terrain through out the country especially in Himalayas,Karakoram and Hindukush (HKH),western border mountains and Balochistan Plateau.This model can be instrumental as baseline geospatial information for scientific investigations in Pakistan,where substantial fraction of national population is living in mountainous regions. 展开更多
关键词 Pakistan distributed modeling ESR DEM GIS rugged terrain
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Trimming the Degrees of Freedom via a K^(+) Flux Rectifier for Safe and Long‑Life Potassium‑Ion Batteries 被引量:2
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作者 Xianhui Yi Apparao MRao +1 位作者 Jiang Zhou Bingan Lu 《Nano-Micro Letters》 SCIE EI CAS CSCD 2023年第11期157-171,共15页
High degrees of freedom(DOF)for K^(+)movement in the electrolytes is desirable,because the resulting high ionic conductivity helps improve potassium-ion batteries,yet requiring support from highly free and flammable o... High degrees of freedom(DOF)for K^(+)movement in the electrolytes is desirable,because the resulting high ionic conductivity helps improve potassium-ion batteries,yet requiring support from highly free and flammable organic solvent molecules,seriously affecting battery safety.Here,we develop a K^(+)flux rectifier to trim K ion’s DOF to 1 and improve electrochemical properties.Although the ionic conductivity is compromised in the K^(+)flux rectifier,the overall electrochemical performance of PIBs was improved.An oxidation stability improvement from 4.0 to 5.9 V was realized,and the formation of dendrites and the dissolution of organic cathodes were inhibited.Consequently,the K||K cells continuously cycled over 3,700 h;K||Cu cells operated stably over 800 cycles with the Coulombic efficiency exceeding 99%;and K||graphite cells exhibited high-capacity retention over 74.7%after 1,500 cycles.Moreover,the 3,4,9,10-perylenetetracarboxylic diimide organic cathodes operated for more than 2,100 cycles and reached year-scale-cycling time.We fabricated a 2.18 Ah pouch cell with no significant capacity fading observed after 100 cycles. 展开更多
关键词 Electrolytes Degrees of freedom safe Coulombic efficiency Potassium-ion batteries
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Bifunctional flame retardant solid-state electrolyte toward safe Li metal batteries 被引量:4
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作者 Qiang Lv Yajie Song +10 位作者 Bo Wang Shangjie Wang Bochen Wu Yutong Jing Huaizheng Ren Shengbo Yang Lei Wang Lihui Xiao Dianlong Wang Huakun Liu Shixue Dou 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2023年第6期613-622,I0014,共11页
Solid polymer electrolytes(SPEs)are one of the most promising alternatives to flammable liquid electrolytes for building safe Li metal batteries.Nevertheless,the poor ionic conductivity at room temperature(RT)and low ... Solid polymer electrolytes(SPEs)are one of the most promising alternatives to flammable liquid electrolytes for building safe Li metal batteries.Nevertheless,the poor ionic conductivity at room temperature(RT)and low resistance to Li dendrites seriously hinder the commercialization of SPEs.Herein,we design a bifunctional flame retardant SPE by combining hydroxyapatite(HAP)nanomaterials with Nmethyl pyrrolidone(NMP)in the PVDF-HFP matrix.The addition of HAP generates a hydrogen bond network with the PVDF-HFP matrix and cooperates with NMP to facilitate the dissociation of Li TFSI in the PVDF-HFP matrix.Consequently,the prepared SPE demonstrates superior ionic conductivity at RT,excellent fireproof properties,and strong resistance to Li dendrites.The assembled Li symmetric cell with prepared SPE exhibits a stable cycling performance of over 1200 h at 0.2 m A cm^(-2),and the solid-state LiFePO_4||Li cell shows excellent capacity retention of 85.3%over 600 cycles at 0.5 C. 展开更多
关键词 Solid polymer electrolytes safe Li metal batteries Li dendrites Hydroxyapatite N-methyl pyrrolidone PVDF-HFP Fireproof property
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The collagen type Ⅰ alpha 1 chain gene is an alternative safe harbor locus in the porcine genome 被引量:1
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作者 XIANG Guang-ming ZHANG Xiu-ling +9 位作者 XU Chang-jiang FAN Zi-yao XU Kui WANG Nan WANG Yue CHE Jing-jing XU Song-song MU Yu-lian LI Kui LIU Zhi-guo 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2023年第1期202-213,共12页
Efficient and stable expression of foreign genes in cells and transgenic animals is important for gain-of-function studies and the establishment of bioreactors.Safe harbor loci in the animal genome enable consistent o... Efficient and stable expression of foreign genes in cells and transgenic animals is important for gain-of-function studies and the establishment of bioreactors.Safe harbor loci in the animal genome enable consistent overexpression of foreign genes,without side effects.However,relatively few safe harbor loci are available in pigs,a fact which has impeded the development of multi-transgenic pig research.We report a strategy for efficient transgene knock-in in the endogenous collagen type I alpha 1 chain(COL1A1)gene using the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9(CRISPR/Cas9)system.After the knock-in of a 2A peptide-green fluorescence protein(2A-GFP)transgene in the last codon of COL1A1 in multiple porcine cells,including porcine kidney epithelial(PK15),porcine embryonic fibroblast(PEF)and porcine intestinal epithelial(IPI-2I)cells,quantitative PCR(qPCR),Western blotting,RNA-seq and CCK8 assay were performed to assess the safety of COL1A1 locus.The qPCR results showed that the GFP knock-in had no effect(P=0.29,P=0.66 and P=0.20 for PK15,PEF and IPI-2I cells,respectively)on the mRNA expression of COL1A1 gene.Similarly,no significant differences(P=0.64,P=0.48 and P=0.80 for PK15,PEF and IPI-2I cells,respectively)were found between the GFP knock-in and wild type cells by Western blotting.RNA-seq results revealed that the transcriptome of GFP knock-in PEF cells had a significant positive correlation(P<2.2e–16)with that of the wild type cells,indicating that the GFP knock-in did not alter the global expression of endogenous genes.Furthermore,the CCK8 assay showed that the GFP knock-in events had no adverse effects(P_(24)h=0.31,P_(48)h=0.96,P_(72)h=0.24,P_(96)h=0.17,and P_(120)h=0.38)on cell proliferation of PK15 cells.These results indicate that the COL1A1 locus can be used as a safe harbor for foreign genes knock-in into the pig genome and can be broadly applied to farm animal breeding and biomedical model establishment. 展开更多
关键词 COL1A1 gene safe harbor KNOCK-IN CRISPR/Cas9 PIG
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Determining safe yield and mapping water level zoning in groundwater resources of the Neishabour Plain 被引量:1
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作者 Parisa Kazerani Ali Naghi Ziaei Kamran Davari 《Journal of Groundwater Science and Engineering》 2023年第1期47-54,共8页
Groundwater is a crucial sources of water supply,especially in arid and semi-arid areas around the world.With uncontrolled withdrawals and limited availability of these resources,it is essential to determine the safe ... Groundwater is a crucial sources of water supply,especially in arid and semi-arid areas around the world.With uncontrolled withdrawals and limited availability of these resources,it is essential to determine the safe yield of these valuable resources.The Hill method approach was used in this study to determine the safe yield the Neishabour aquifer in Khorasan Razvi province in Iran.The results showed that the safe yield in the Neishabour aquifer is 60%lower than the current pumping amounts during the study period,indicating that further overdrafts could result in the destruction of this aquifer.This highlights the importance of using the Hill method to estimate the permitted exploitation from other aquifers,thus preventing problems caused by over-extraction and maintaining stability of global groundwater levels. 展开更多
关键词 Hill method Water level zoning maps Groundwater pumping safe yield Groundwater crisis
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Application of Gaussian beam pre-stack depth migration in rugged seabed
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作者 HAN Fuxing YI Xin +3 位作者 SUN Zhangqing HU Jia XU Baoyin XU Hai 《Global Geology》 2021年第2期119-128,共10页
Strong fluctuation of seabed,abrupt variation in depth and dip of seabed bring seismic imaging problems,such as irregular reflection waves,obvious multiple waves,serious lateral wave development,poor imaging on base s... Strong fluctuation of seabed,abrupt variation in depth and dip of seabed bring seismic imaging problems,such as irregular reflection waves,obvious multiple waves,serious lateral wave development,poor imaging on base surface and depression structure,low signal-to-noise ratio of middle and deep layers.In this paper,Gaussian beam migration imaging method is used to analyze the imaging effect of rugged seabed in deep water area,and the ray tracing method of wavefront construction method is used to analyze the kinematic characteristics of seismic waves.By improving the design of seismic data acquisition and observation system,imaging quality of fine structures is improved. 展开更多
关键词 Gaussian beam migration wavefront construction method Gaussian beam pre-stack depth rugged seabed
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Morbi-Mortality Linked to Unsafe Abortions—Difficulties in Accessing Safe Abortions in Cameroon: Meta-Analysis and Systematic Review
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作者 Florent Ymele Fouelifack Mosman Anyimbi Ofeh +5 位作者 Jenny Ornella Manewoun Nsen Abeng Christophe Saha Lontsi Mvong Vendeline Amaelle Goretti Guy Sadeu Wafeu Robinson Enow Mbu 《Open Journal of Obstetrics and Gynecology》 CAS 2023年第2期342-359,共18页
Background: Unsafe abortions are one of the leading causes of maternal mortality, especially in developing countries. In Cameroon, the maternal mortality rate remains high, and the scarcity of data on abortions leads ... Background: Unsafe abortions are one of the leading causes of maternal mortality, especially in developing countries. In Cameroon, the maternal mortality rate remains high, and the scarcity of data on abortions leads to a lack of solid evidence to advocate on the extent of the abortions related complications. Our objective was to evaluate the unsafe abortions related complications, and to assess the difficulties of accessing safe abortions in our setting. Methods: We carried out a meta-analytic and systematic review in the biomedical databases MEDLINE (Pubmed), Google Scholar and African Journal Online concerning unsafe abortions and/or difficulties in accessing safe abortions in Cameroon. The keywords used for the search are seen in table I. Selection of studies was simultaneously done by two authors. Data were extracted through a form designed on Google Form. We used a random-effect model for proportion estimation, and The I<sup>2</sup> and Q statistics to assess the extent of heterogeneity. Results: A total of 430 studies were identified, from which 28 were included and analysed. About 5% (95% CI: 3 - 7) of unsafe abortions leads to death. The contribution of unsafe abortions in maternal deaths was 23% (95% CI: 20 - 27). The rate of severe bleeding and/or anemia were 40% (95% CI: 18 - 63) and the rate of infection was 17% (95% CI: 7 - 28), dominated by pelvic infections, pelviperitonitis, severe sepsis, and septic shock. Case reports described uterine perforations, uterine rupture during the following pregnancy. Abortion was performed in the practitioner’s or patient’s home in 41.4% of cases, in a health center in 35.1% of cases, in a private clinic in 21.2% of cases, drugs selling places and in traditional healer clinics. The restriction of abortion laws, the stigma surrounding abortion and its consequences at any level of the society, lead to the underreporting of unsafe abortions and a deep reluctance to advocate for safe abortion services. Conclusion: The strengthening of awareness campaigns for provider behavior change communication, family planning, the de-stigmatization of abortions, the training of health personnel in post-abortion care, a multidisciplinary and multicentric action would contribute to the reduction in morbidity and mortality due to abortions. 展开更多
关键词 Morbi-Mortality Unsafe DIFFICULTIES Access safe ABORTION Cameroon
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