Effect of Wearing a Maxillofacial Prosthesis on Masticatory Rhythm Improvement in Mandibulectomy Patients

Background: Recording jaw movement rhythms in patients with jaw defects is challenging using conventional tracking devices. However, masticatory rhythm is crucial for masticatory performance and nutritional intake. This study aimed to explore the impact of using a maxillofacial prosthesis to restore masticatory rhythm in mandibulectomy patients with a novel wearable device. Methods: Twelve patients who underwent mandibulectomy and were rehabilitated with maxillofacial prosthesis were recruited. Chewing rate, cycle durations, coefficient of variation of cycle durations (CV), changing times of skin morphology on the back of the ear pinna area (SM), and masticatory performance were measured during gum chewing without and with a denture using a wearable jaw movement rhythm tracking device. A paired t-test and the Pearson correlation coefficient were used for statistical analysis. Results: The chewing rate increased by 8.6 cycles/min with dentures (P P = 0.01). The CV and SM decreased with dentures (P = 0.004 and P = 0.01, respectively). Significant correlations were found between the CV and SM without dentures (P = 0.004). Conclusion: 1. Wearing maxillofacial prostheses can improve masticatory rhythm in patients who undergo mandibulectomy with Eichner B2 occlusal supports. 2. The wearable device is available for measuring masticatory rhythm in patients with jaw defects in clinics. 3. Changing times of the skin morphology on the back of the ear pinna indicate jaw movement stability through a simpler process than the coefficient of variation of cycle durations.

Probiotics administration alleviates cognitive impairment and circadian rhythm disturbance induced by sleep deprivation

Gut microbiome is indispensable for maintaining normal brain function.Specifically,gut microbiota plays a causal role in sleep deprivation(SD)-induced cognitive impairment.In this study,neurobehavioral effects of the Bifidobacterium breve strain(CCFM1025)were assessed in sleep-deprived mice.CCFM1025 improved the body weight and food and water intake of the mice.It also alleviated SD-induced cognitive behavioural abnormalities(in the novel object recognition test),but did not show beneficial effects on mood-and spatial memory-related behaviours.CCFM1025 significantly altered the gut microbial composition and genome function.Key microbial metabolites that may regulate sleep function were also identified,such as isovaleric acid andγ-aminobutyric acid in the gut and purine metabolites in the serum.Those metabolites may participate in gutbrain communication by acting on the striatal melatonin system,for example to increase melatonin levels,and by regulating the expression of circadian clock genes such as those encoding the adenosine A2A receptor and period circadian regulator 1.Collectively,administration of probiotics alleviated cognitive impairment and circadian rhythm disturbance induced by SD via modulation of gut microbiome and its metabolites.These findings may help guide the treatment of insomnia or other sleep disorders via dietary strategies.

Circadian rhythm disruption and retinal dysfunction:a bidirectional link in Alzheimer’s disease?

Dysfunction in circadian rhythms is a common occurrence in patients with Alzheimer’s disease.A predominant function of the retina is circadian synchronization,carrying information to the brain through the retinohypothalamic tract,which projects to the suprachiasmatic nucleus.Notably,Alzheimer’s disease hallmarks,including amyloid-β,are present in the retinas of Alzheimer’s disease patients,followed/associated by structural and functional disturbances.However,the mechanistic link between circadian dysfunction and the pathological changes affecting the retina in Alzheimer’s disease is not fully understood,although some studies point to the possibility that retinal dysfunction could be considered an early pathological process that directly modulates the circadian rhythm.

Autophagy and circadian rhythms:interactions and clinical implications

Autophagy is a widespread biological process that controls cellular growth,survival,development,and death.Circadian rhythm is a recurring reaction of living organisms and behaviors to variations in surrounding brightness and obscurity.Most of the fundamental physiological processes in mammals,such as the sleep-wake pattern and the rhythm of nutrition and energy metabolism,are governed by circadian rhythms.Research has indicated that autophagy exhibits a specific circadian pattern in both normal and abnormal conditions.Autophagy can modulate circadian rhythms by breaking down proteins that regulate the circadian clock.The potential regulatory connection between the two has been a popular subject of clinical and fundamental research.Understanding the interaction between circadian rhythm and autophagy could potentially lead to the development of novel approaches for disease treatment in the future.The present analysis presented a summary of the molecular processes implicated in the interplay between autophagy and circadian rhythm,as well as the pathological importance of the disrupted regulatory association between these two phenomena.

The modulatory effect of oolong tea polyphenols on intestinal flora and hypothalamus gene expression in a circadian rhythm disturbance mouse model

The interaction between host circadian rhythm and gut microbes through the gut-brain axis provides new clues for tea polyphenols to improve host health.Our present research showed that oolong tea polyphenols(OTP)improved the structural disorder of the intestinal flora caused by continuous darkness,thereby modulating the production of metabolites related to pyruvate metabolism,glycolysis/gluconeogenesis,and tryptophan metabolism to alleviate the steady-state imbalance.After fecal microbiota transplantation from the OTP group,the single-cell transcriptomic analysis revealed that OTP significantly increased the number of hypothalamus cell clusters,up-regulated the number of astrocytes and fibroblasts,and enhanced the expression of circadian rhythm genes Cry2,Per3,Bhlhe41,Nr1d1,Nr1d2,Dbp and Rorb in hypothalamic cells.Our results confirmed that OTP can actively improve the intestinal environmental state as well as internal/peripheral circadian rhythm disorders and cognitive impairment,with potential prebiotic functional characteristics to notably contribute to host health.

Unraveling links between aging,circadian rhythm and cancer:Insights from evidence-based analysis

Aging and circadian rhythms have been connected for decades,but their molecular interaction has remained unknown,especially for cancers.In this situation,we summarized the current research actuality and problems in this field using the bibliometric analysis.Publications in the PubMed and Web of Science databases were retrieved.Overall,there is a rising trend in the publication volume regarding aging and circadian rhythms in the field of cancer.Researchers from USA,Germany,Italy,China and England have greater studies than others.Top three publication institutions are University of California System,UDICE-French Research Universities and University of Texas System.Current research hotspots include oxidative stress,breast cancer,melatonin,cell cycle,calorie restriction,prostate cancer and NF-κB.In conclusion,results generated by bibliometric analysis indicate that many approaches involve in the complex interactions between aging and circadian rhythm in cancer.These established and emerging research directions guide our exploration of the regulatory mechanisms of aging and circadian rhythms in cancer and provide a reference for developing new research avenues.

Interaction between adolescent sleep rhythms and gender in an obese population

BACKGROUND The obesity rate of adolescents is gradually increasing,which seriously affects their mental health,and sleep plays an important role in adolescent obesity.AIM To investigate the relationship between sleep rhythm and obesity among adolescents and further explores the interactive effect of sleep rhythm and gender on adolescent obesity,providing a theoretical basis for developing interventions for adolescent obesity.METHODS Research data source Tianjin Mental Health Promotion Program for Students.From April to June 2022,this study selected 14201 students from 13 middle schools in a certain district of Tianjin as the research subject using the convenient cluster sampling method.Among these students,13374 accepted and completed the survey,with an effective rate of 94.2%.The demographic data and basic information of adolescents,such as height and weight,were collected through a general situation questionnaire.The sleep rhythm of adolescents was evaluated using the reduced version of the morningness-eveningness questionnaire.RESULTS A total of 13374 participants(6629 females,accounting for 49.56%;the average age is 15.21±1.433 years)were analyzed.Among them,the survey showed that 2942 adolescent were obesity,accounting for 22%and 2104 adolescent were overweight,accounting for 15.7%.Among them,1692 male adolescents are obese,with an obesity rate of 25.1%,higher than 18.9%of female adolescents.There is a statistically significant difference between the three groups(χ2=231.522,P<0.000).The obesity group has the smallest age(14.94±1.442 years),and there is a statistical difference in age among the three groups(F=69.996,P<0.000).Obesity rates are higher among individuals who are not-only-child,have residential experience within six months,have family economic poverty,and have evening-type sleep(P<0.05).Logistic regression analysis shows a correlation between sleep rhythm and adolescent obesity.Evening-type sleep rhythm can increase the risk of obesity in male adolescents[1.250(1.067-1.468)],but the effect on female obesity is not remarkable.Further logistic regression analysis in the overall population demonstrates that the interaction between evening-type sleep rhythm and the male gender poses a risk of adolescent obesity[1.122(1.043-1.208)].CONCLUSION Among adolescents,the incidence of obesity in males is higher than in females.Evening-type sleep rhythm plays an important role in male obesity but has no significant effect on female obesity.Progressive analysis suggests an interactive effect of sleep rhythm and gender on adolescent obesity,and the combination of evening-type sleep and the male gender promotes the development of adolescent obesity.In formulating precautions against adolescent obesity,obesity in male adolescents with evening-type sleep should be a critical concern.

Adjusting phosphate feeding regimen according to daily rhythm increases eggshell quality via enhancing medullary bone remodeling in laying hens

Background Body phosphorus metabolism exhibits a circadian rhythm over the 24-h daily cycle.The egg laying behavior makes laying hens a very special model for investigating phosphorus circadian rhythms.There is lack of information about the impact of adjusting phosphate feeding regimen according to daily rhythm on the phosphorus homeostasis and bone remodeling of laying hens.Methods and results Two experiments were conducted.In Exp.1,Hy-Line Brown laying hens(n=45)were sampled according the oviposition cycle(at 0,6,12,and 18 h post-oviposition,and at the next oviposition,respectively;n=9 at each time point).Diurnal rhythms of body calcium/phosphorus ingestions and excretions,serum calcium/phosphorus levels,oviduct uterus calcium transporter expressions,and medullary bone(MB)remodeling were illustrated.In Exp.2,two diets with different phosphorus levels(0.32%and 0.14%non-phytate phosphorus(NPP),respectively)were alternately presented to the laying hens.Briefly,four phosphorus feeding regimens in total(each included 6 replicates of 5 hens):(1)fed 0.32%NPP at both 09:00 and 17:00;(2)fed 0.32%NPP at 09:00 and 0.14%NPP at 17:00;(3)fed 0.14%NPP at 09:00 and 0.32%NPP at 17:00;(4)fed 0.14%NPP at both 09:00 and 17:00.As a result,the regimen fed 0.14%NPP at 09:00 and 0.32%NPP at 17:00,which was designed to strengthen intrinsic phosphate circadian rhythms according to the findings in Exp.1,enhanced(P<0.05)MB remodeling(indicated by histological images,serum markers and bone mineralization gene expressions),elevated(P<0.05)oviduct uterus calcium transportation(indicated by transient receptor potential vanilloid 6 protein expression),and subsequently increased(P<0.05)eggshell thickness,eggshell strength,egg specific gravity and eggshell index in laying hens.Conclusions These results underscore the importance of manipulating the sequence of daily phosphorus ingestion,instead of simply controlling dietary phosphate concentrations,in modifying the bone remodeling process.Body phosphorus rhythms will need to be maintained during the daily eggshell calcification cycle.

Circadian Rhythm of Childbirths and Maternal and Neonatal Prognosis at the YaoundéCentral Hospital

Background: The circadian variation of childbirths has been described by several authors around the globe. De Graaf showed that hospital childbirths at night were associated with increased perinatal mortality and adverse perinatal outcome. To improve obstetric care, we carried out this study to evaluate the circadian rhythm of childbirths and to assess the outcome following variations in the time of childbirths during the day. Methods: It was a cross-sectional descriptive study at the Yaoundé Central Hospital (YCH), over a two years period. We collected data from files of women who delivered from the 1st of January 2017 to 31st December 2018. We included files of women who delivered at least at 28 weeks of pregnancy. We excluded files of those who delivered by elective caesarean section, those whose hour of delivery was not noted and those who delivered before reaching the hospital. Sociodemographic, obstetrical characteristics, and immediate prognosis were recorded. Data were entered into excel, then analysed with SPSS v23 software. Tools used to appreciate our results were means, median, number, percentage, P, and OR with its 95% confidence interval. The difference in p is significant if p is less than 5%. Results: We analyzed 6041 files bearing the time of birth. Childbirths took place at all hours of the day, but the times of the day where the highest numbers of births were recorded were 10, 11, 12, 13 (that’s 1pm), 14 (that’s 2 pm), 15 (that’s 3 pm), 16 (that’s 4 pm), 17 (that’s 5 pm) and 23 (that’s 11 pm) hours, with respectively 224 (3.7%), 277 (4.6%), 256 (4.2%), 265 (4.4%), 207 (3.4%), 255 (4.2%), 228 (3.8%), 216 (3.6%) and 226 (3.7%) births. The peak of births was at 11 am while the time of day where the lowest number of births was recorded was 6 pm, with 175 (2.9%) births. The mean age of participants was 27.34 ± 6.03 years with extremes of 13 and 49, with 87.6% between 20 to 39 years. Sociodemographic characteristics of participants, prematurity and bleeding during delivery, had no dependence on the time of delivery. Perineal tear, duration of observation, Apgar score of the newborn, birthweight, delivery mode, health personnel who performed the delivery, and episiotomyseemed to be influenced by the time of delivery. Daily shifts were not independently associated with the poor Apgar score (0 - 6) at 5 mins, when adjusted for all other factors (p = 0.109). Conclusion: Childbirths were more frequent between 10 am and 5 pm. The period where episiotomy was most performed is the same as when there was macrosomia childbirth. Tears of the perineum are more frequent between 2 pm and 10 pm. There was no independent association between Daily shifts and poor Apgar score. The poor APGAR score would be more related to low birth weight.

Potential Power of the Pyramidal Structure VII: Effects of Pyramid Power and Bio-Entanglement on the Circadian Rhythm of Biosensors

We have demonstrated the existence of a pyramid power and have revealed its characteristics by strictly scientific experiments using biosensors. We also revealed the existence of a Bio-Entanglement, an entangled relationship between biosensors. A parallel study of biosensors (edible cucumber slices) had also been conducted, and we found that the circadian rhythm of gas concentrations emitted from biosensors changes seasonally. The pyramid power and Bio-Entanglement did not change the number of cycles in the periodic approximation curve representing circadian rhythm. Therefore, in this paper we analyzed the influence of the pyramid power and Bio-Entanglement, i.e., their influence on the phase, amplitude, and correlation coefficient of the periodic approximation curve representing the circadian rhythm of emitted gas concentrations. The main results are as follows. 1) The pyramid power shifted the phase of the periodic approximation curve representing the circadian rhythm by 43 minutes. 2) The amplitude of the periodic approximation curve changed with the pyramid power and the Bio-Entanglement. The effect on the lower and upper sections of the biosensors stacked in two layers was different, with a tendency to increase the amplitude of the lower layer and decrease the amplitude of the upper layer. 3) The pyramid power and the Bio-Entanglement affected the correlation coefficient between gas concentration and the periodic approximation curve representing the circadian rhythm of gas concentration. The effect on the lower and upper layers of the biosensors was different, with a tendency for the lower layer correlation coefficient to be larger and the upper layer correlation coefficient to be smaller. Previously we demonstrated that the pyramid power and the Bio-Entanglement affect the ratio of gas concentration, i.e., psi index Ψ. In this paper we demonstrate for the first time that the pyramid power and the Bio-Entanglement affect time, i.e., phase difference.

Effects of constant light and dark conditions on the locomotor activity,body mass,and body temperature rhythms of Eurasian Tree Sparrows(Passer montanus)

Light is an essential environmental cue influencing the endogenous circadian clocks that regulate behavioral and physiological processes in animals.Despite extensive research on the circadian rhythms of avian behavior and physiology,the mechanisms by which they adapt and adjust to abnormal photoperiod conditions,such as artificial light,have yet to be fully elucidated.Here,the circadian rhythms of the body mass,activity,and core and peripheral body temperatures of the Eurasian Tree Sparrow(Passer montanus,ETS)-a diurnal passerine-were examined under three different lighting conditions:constant light(LL),constant darkness(DD),and normal light-dark cycles(LD).Our results showed that the activity,body mass,core,tarsometatarsus,and eye temperatures of the ETSs varied significantly with treatment,time,and the interaction between treatment and time;however,there was no significant change in body mass in response to the interaction between time and treatment.Under LD conditions,the ETSs exhibited significant circadian rhythms in activity and core,tarsometatarsus,and eye temperatures.Under LL conditions,only the core temperature exhibited significant rhythmicity.Under DD conditions,there were no significant circadian rhythms in activity,body mass,and eye temperature;however,the core and tarsometatarsus temperatures remained rhythmic.Our results indicate that the core body temperature rhythm of ETS is endogenous and can be maintained under abnormal photoperiods.In contrast,the activity and body surface temperature,uncoupled from the core body temperature under abnormal photoperiod conditions,strongly depend on light.These results differ from those of other diurnal avian species,suggesting a unique coping mechanism of ETS in response to an abnormal photoperiod.

生物钟在口腔医学研究中合理应用专家共识

机体生物钟(也称昼夜节律)是地球上所有生物体能适者生存在地球自转环境中的基础。昼夜节律是生命活动最基本的调控机制,在维持人体正常生理生化稳态、疾病发生及治疗中均扮演关键角色。研究表明,生物钟在口腔组织发育生长、口腔疾病发生及治疗中具有重要作用。由于目前还没有包括口腔医学在内的生物钟研究方法的指导性文献,研究者主要基于已发表的参考文献进行探讨,致使口腔医学中有关生物钟的研究方法存在争议,如何合理应用昼夜节律的研究方法还存在诸多困惑。该共识在总结生物钟的作用特点以及分析目前生物钟在口腔医学研究中不足的基础上,组织相关专家归纳推荐了生物钟在口腔医学研究中合理实施的10条原则,为生物钟在口腔医学研究中的合理应用提供参考。

论现代汉语虚词与穆旦诗歌节奏的建构

大量虚词入诗对穆旦诗歌节奏有根本性影响:虚词改变了传统诗歌双音步为主的节奏机制,建构了灵活多变的超音步节奏机制;打破了汉语诗歌“连续强重音”状态,形成具有轻重起伏和快慢交替的语音节奏。助词、介词等虚词的连续重复或间隔重复,构成绵密、对称、整齐的视觉节奏,介词宾语的多变体现了现代汉语诗歌节奏“有约束的自由”的特征;虚词“同位重复”的布局形成结构相同、平行展开的意群或诗节,构成均齐的视觉节奏。虚词与实词相结合起到情感和意义“增值”的审美作用;穆旦诗歌中情绪节奏与语思节奏密不可分,二者均呈现出复杂,多重,多向,多变的特征,形成“多声部”、多变的节奏。穆旦诗歌节奏是立体多元的现代诗歌节奏,穆旦竭力在传统诗歌节奏和西方诗歌节奏之间探索出一条适合现代汉语的“声音”之路。

大学生昼夜节律与睡眠质量的关系:特质焦虑和睡眠信念态度的链式中介作用

目的探究在大学生群体中昼夜节律类型与睡眠质量之间的关系,明确特质焦虑和睡眠信念态度在其中所起的中介作用。方法采用便利抽样的方法对某医学院大学生进行线上调查,使用清晨型与夜晚型量表-5项(MEQ-5)、特质焦虑量表(T-AI)、简易睡眠信念和态度量表(DBAS-16)、匹兹堡睡眠质量指数量表(PSQI)分别评估大学生的昼夜节律类型、特质焦虑水平、睡眠信念态度和睡眠质量。采用Pearson相关分析和中介效应分析探讨昼夜节律类型、特质焦虑水平、睡眠信念态度和睡眠质量之间的关系。结果共回收问卷238份,有效问卷233份,有效率为97.9%。Pearson相关分析结果显示昼夜节律类型、特质焦虑水平、睡眠信念态度和睡眠质量4个变量两两之间呈显著相关(均P<0.01)。以昼夜节律类型为自变量、睡眠质量为因变量、特质焦虑水平和睡眠信念态度为中介变量进行中介效应分析,结果显示特质焦虑水平的中介效应量(MEQ-5→T-AI→PSQI)占总效应的30.48%(r=-0.128,95%CI-0.211~-0.066),睡眠信念态度的中介效应量(MEQ-5→DBAS-16→PSQI)占总效应的12.62%(r=-0.053,95%CI-0.106~-0.014),特质焦虑水平和睡眠信念态度的链式中介效应量(MEQ-5→T-AI→DBAS-16→PSQI)占总效应的11.19%(r=-0.047,95%CI-0.085~-0.022)。结论大学生的昼夜节律类型能够负向预测其睡眠质量,特质焦虑水平和睡眠信念态度在其中起链式中介作用。

屏蔽蓝光干预对双相障碍维持治疗期患者的生物节律疗效与安全性

目的:使用屏蔽蓝光附加治疗对伴有失眠症状的双相障碍维持治疗期患者进行干预,探究其对双相障碍患者生物节律及睡眠的疗效与安全性。方法:选取2020年9月至2023年4月在上海市虹口区精神卫生中心符合纳入标准的双相障碍患者45例,随机分为干预组22例和对照组23例。在保持原来治疗方案不变基础上,干预组患者额外每晚不少于2 h佩戴防蓝光的橙色眼镜持续4周。两组患者于基线、第2周末、第4周末进行中文版神经精神科生物节律评估访谈(BRIAN-C)、匹兹堡睡眠质量指数(PSQI)评估。结果:两组之间比较的重复测量方差分析显示B RIAN-C评分(F时间=17.257,P=0.000)、PSQI评分(F时间=17.144,P=0.000)时间效应均显著。第4周末两组之间BRIAN-C量表主导节律因子分差异具有统计学意义(Z=-2.266,P<0.05)。两组患者均未报告研究相关不良事件发生。结论:屏蔽蓝光附加治疗有效改善双相障碍维持治疗期患者的生物节律紊乱及失眠症状,且安全性和耐受性好。

生物节律与子痫前期的相关性

子痫前期(preeclampsia,PE)是一种常见的妊娠并发症,危害母婴健康,更是一个严重的国际公共卫生问题。尽管已证实PE的高危因素包括肥胖、糖尿病和妊娠前高血压等,但其发病机制仍未完全阐明。哺乳动物中绝大多数的生理过程由生物节律调控,生物节律的破坏被证实与众多疾病相关,如心血管疾病、糖尿病和乳腺癌等。尽管PE具有一系列时间生物节律的特征性表现,如PE患者的血压24 h节律变异性、“反勺状”血压与更严重的靶器官损伤有关、夜间服用阿司匹林的预防保护作用更优异等,但生物节律的变异是否为PE的危险因素,抑或PE本身是否与异常的生物节律相关,目前尚未明确。综述生物节律与PE的相关性,包括胎盘的钟基因表达及其与PE的关系、血压的生物节律、PE预防的时间治疗学、生物节律的破坏与PE风险性,以启示今后深入研究生物节律/钟基因与PE之间潜在关联的必要性。

战略节奏推动酒店企业惯例更新的过程与机制——以本土酒店碧水湾为例

伴随着中国经济全面而深刻的转型和数字技术的快速应用与发展,如何实现惯例更新成为酒店业转型发展的关键议题。文章以本土酒店碧水湾为研究对象,运用纵向案例研究方法,基于“认知-行动-结果”的理论逻辑,剖析酒店企业通过战略节奏推动惯例更新的动态过程与作用机制。研究发现:(1)高管认知是互动行为产生的根源,两者之间存在适配机制塑造战略节奏。高管通过对外部环境的分析形成了经验导向型认知、市场导向型认知和技术导向型认知,而与其相适配的互动行为分别为基于认知引导的互动行为、基于文化桥梁的互动行为和基于业务编排的互动行为。(2)战略节奏推动酒店惯例更新,需循“储势蓄能-调整转变-发展升级”的演化轨迹,在不同阶段呈现“快速度低变异-快速度高变异-慢速度低变异”的战略节奏特征。(3)高管认知和与之相适配的互动行为塑造战略节奏,这一过程作用于组织惯例更新,从构建新惯例制度结构,向形成新惯例流程数字化,再向固化新惯例业务数字化演变。文章创新性构建并解析了战略节奏推动酒店惯例更新的理论框架,拓宽了战略节奏和组织惯例研究理论,为本土酒店企业转型和产业结构高质量发展提供了案例借鉴。

药食同源理论及其在航天营养健康研究中的应用

载人航天飞行中面临的重力场变化、辐射、狭小空间孤闭隔离、噪声及生物节律变化等航天特因环境给维持航天员机体营养健康带来了挑战。根据载人航天实践,综述了航天特因环境下机体适应性变化,阐述了药食同源在航天营养健康保障中的应用预先研究现状,分析并提出了药食同源在支持未来载人深空探测方面的潜在应用价值。

2023年美国心房颤动指南解读——心房颤动导管消融

2023年美国心脏病学会(ACC)、美国心脏协会(AHA)、美国胸科医师学会(ACCP)和美国心律学会(HRS)心房颤动(房颤)诊断与治疗指南,提出对房颤患者尽早干预和持续治疗,强调早期节律控制的重要性。近年的研究证实无论持续性房颤还是阵发性房颤,导管消融的疗效均优于抗心律失常药物,且尽早实施节律控制策略是提高房颤导管消融成功率的重要因素。对于经选择的房颤患者,新指南将导管消融提升为节律控制的一线治疗策略。从长远来看,相对年轻的房颤患者从导管消融中获益更多。在消融策略方面,对于所有接受导管消融的房颤患者,肺静脉隔离是首要目标,其他消融终点目前证据不足。

生物钟基因Bmal1、Per2在血液肿瘤细胞株Raji、Hut-78、OCI-LY8及HL-60中的昼夜表达规律

目的通过检测生物钟基因Bmal1、Per2在血液肿瘤细胞株Raji、Hut-78、OCI-LY8及HL-60细胞中不同时间点的mRNA表达情况,探讨其与血液肿瘤发生发展的可能关系。方法血液肿瘤细胞株Raji、Hut-78、LY-8、HL-60经血清休克法诱导节律后,RT-PCR法检测生物钟基因Bmal1及Per2在不同时间点的mRNA转录水平,以时间为变量分别对其转录量进行余弦函数拟合,通过余弦函数预测基因转录高峰及低谷时段。结果Bmal1和Per2基因表达在OCI-LY8、Hut-78、HL-60细胞中呈现昼夜节律(P<0.05);Raji细胞内Bmal1基因无节律性表达(P>0.05),而Per2基因呈昼夜节律表达(P<0.05)。结论生物钟基因Per2和(或)Bmal1在多种血液肿瘤细胞株内呈昼夜节律性表达,其可能参与血液肿瘤的发生发展。