Objective:To study the correlation of Runt-related transcription factor gene 3 (RUNX3) expression in osteosarcoma tissue with cell proliferation and angiogenesis.Methods: A total of 80 patients with osteosarcoma who w...Objective:To study the correlation of Runt-related transcription factor gene 3 (RUNX3) expression in osteosarcoma tissue with cell proliferation and angiogenesis.Methods: A total of 80 patients with osteosarcoma who were treated in our hospital between February 2014 and February 2017 were collected, and the RUNX3 expression in osteosarcoma tissue and adjacent tissue were detected. According to the RUNX3 expression in tumor tissue, the patients were further divided into high RUNX3 expression group and low RUNX3 expression group, and the proliferation gene and angiogenesis gene expression were compared.Results:RUNX3, KISS-1 and RanBP9 mRNA expression in osteosarcoma tissue were significantly lower than those in adjacent tissue while VCP, Six1, S100A6, IF-1α, MMP-14, bFGF and Ang-2 mRNA expression were significantly higher than those in adjacent tissue;KISS-1 and RanBP9 mRNA expression in osteosarcoma tissue of high RUNX3 expression group were significantly higher than those of low RUNX3 expression group while VCP, Six1, S100A6, IF-1 , MMP-14, bFGF and Ang-2 mRNA expression were significantly lower than those of low RUNX3 expression group.Conclusions:The desease of RUNX3 expression in osteosarcoma tissue is one of the direct causes of increased tumor proliferation activity and strong angiogenesis.展开更多
Objective: To investigate the correlation of Runt-related transcription factor 2 (RunX2) with proliferation genes, tumor suppressor genes and angiogenesis molecules in colon cancer lesions. Methods: A total of 90 pati...Objective: To investigate the correlation of Runt-related transcription factor 2 (RunX2) with proliferation genes, tumor suppressor genes and angiogenesis molecules in colon cancer lesions. Methods: A total of 90 patients with primary colon cancer were enrolled in colon cancer group, 68 patients with benign colon polyps were enrolled in colon polyps group, the differences in the expression levels of RunX2, proliferation genes, tumor suppressor genes and angiogenesis molecules in the two groups of lesions were compared, and Pearson test was further used to evaluate the correlation of RunX2 expression level with proliferation gene, tumor suppressor gene and angiogenesis molecule expression levels in colon cancer tissues. Results: RunX2 mRNA expression level in the lesions of colon cancer group was higher than that of colon polyps group. Proliferation genes GTPBP4, HOXB7, ZNF331, ADAM17 and HSP60 mRNA expression levels in the lesions of colon cancer group were higher than those of colon polyps group;tumor suppressor genes ATF3, FOXN3, OTUD1 and NDRG2 mRNA expression levels were lower than those of colon polyps group;angiogenesis molecules Musashi 1, NF-κB, RegⅣ and STAT3 mRNA expression levels were higher than those of colon polyps group. RunX2 mRNA expression level in the colon cancer lesions was directly correlated with the expression levels of the above proliferation genes, tumor suppressor genes and angiogenesis molecules. Conclusion: RunX2 expression is abnormally high in colon cancer lesions, the specific expression level is positively correlated with cancer cell proliferation activity and angiogenesis activity, and it is an important molecular target that can lead to the occurrence and development of colon cancer.展开更多
BRI1-EMS-SUPPRESSOR 1(BES1)transcription factor is closely associated with the brassinosteroid(BR)signaling pathway and plays an important role in plant growth and development.SLB3 is a member of BES1 transcription fa...BRI1-EMS-SUPPRESSOR 1(BES1)transcription factor is closely associated with the brassinosteroid(BR)signaling pathway and plays an important role in plant growth and development.SLB3 is a member of BES1 transcription factor family and its expression was previously shown to increase significantly in tomato seedlings under drought stress.In the present study,we used virus-induced gene silencing(VIGS)technology to downregulate SLB3 expression to reveal the function of the SLB3 gene under drought stress further.The downregulated expression of SLB3 weakened the drought tolerance of the plants appeared earlier wilting and higher accumulation of H2 O2 and O2^–·,decreased superoxide dismutase(SOD)activity,and increased proline(PRO)and malondialdehyde(MDA)contents and peroxidase(POD)activity.Quantitative real-time PCR(qRT-PCR)analysis of BR-related genes revealed that the expression of SlCPD,SlDWARF and BIN2-related genes was significantly upregulated in SLB3-silenced seedlings under drought stress,but that the expression of TCH4-related genes was downregulated.These results showed that silencing the SLB3 gene reduced the drought resistance of tomato plants and had an impact on the BR signaling transduction which may be probably responsible for the variation in drought resistance of the tomato plants.展开更多
The B3 transcription factors(TFs)in plants play vital roles in numerous biological processes.Although B3 genes have been broadly identified in many plants,little is known about their potential functions in mediating s...The B3 transcription factors(TFs)in plants play vital roles in numerous biological processes.Although B3 genes have been broadly identified in many plants,little is known about their potential functions in mediating seed development and material accumulation.Castor bean(Ricinus communis)is a non-edible oilseed crop considered an ideal model system for seed biology research.Here,we identified a total of 61 B3 genes in the castor bean genome,which can be classified into five subfamilies,including ABI3/VP1,HSI,ARF,RAV and REM.The expression profiles revealed that RcABI3/VP1 subfamily genes are significantly up-regulated in the middle and later stages of seed development,indicating that these genes may be associated with the accumulation of storage oils.Furthermore,through yeast one-hybrid and tobacco transient expression assays,we detected that ABI3/VP1 subfamily member RcLEC2 directly regulates the transcription of RcOleosin2,which encodes an oil-body structural protein.This finding suggests that RcLEC2,as a seed-specific TF,may be involved in the regulation of storage materials accumulation.This study provides novel insights into the potential roles and molecular basis of B3 family proteins in seed development and material accumulation.展开更多
High temperature stress is one of the major environmental factors that affect the growth and development of plants. Although WRKY transcription factors play a critical role in stress responses, there are few studies o...High temperature stress is one of the major environmental factors that affect the growth and development of plants. Although WRKY transcription factors play a critical role in stress responses, there are few studies on the regulation of heat stress by WRKY transcription factors,especially in tomato. Here, we identified a group I WRKY transcription factor, SlWRKY3, involved in thermotolerance in tomato. First, SlWRKY3 was induced and upregulated under heat stress. Accordingly, overexpression of SlWRKY3 led to an increase, whereas knock-out of SlWRKY3 resulted in decreased tolerance to heat stress. Overexpression of SlWRKY3 accumulated less reactive oxygen species(ROS), whereas knock-out of SlWRKY3 accumulated more ROS under heat stress. This indicated that SlWRKY3 positively regulates heat stress in tomato. In addition,SlWRKY3 activated the expression of a range of abiotic stress-responsive genes involved in ROS scavenging, such as a SlGRXS1 gene cluster.Further analysis showed that SlWRKY3 can bind to the promoters of the SlGRXS1 gene cluster and activate their expression. Collectively, these results imply that SlWRKY3 is a positive regulator of thermotolerance through direct binding to the promoters of the SlGRXS1 gene cluster and activating their expression and ROS scavenging.展开更多
We successfully identified a novel and unique OsbZIP transcription factor,OsbZIP09,whose mutants exhibited longer seeds and less severe pre-harvest sprouting than the wild type,but shared similar germination rate as t...We successfully identified a novel and unique OsbZIP transcription factor,OsbZIP09,whose mutants exhibited longer seeds and less severe pre-harvest sprouting than the wild type,but shared similar germination rate as the wild type under normal germination conditions.The expression of OsbZIP09 was induced by abscisic acid(ABA)and declined as the germination process.As a nucleus-localized transcription factor,the conserved binding motif of OsbZIP09 was identified via DNA affinity purification sequencing technique.Further evidences indicated that OsbZIP09 directly enhanced the expression of ABA catabolism gene ABA8ox1,thus reducing ABA accumulation.In addition,OsbZIP09 also directly bound to the promoter of LEA3 gene to inhibit its expression,thus further alleviating the suppressive effect of ABA on seed germination.These results demonstrated that OsbZIP09 likely functions as a brake of the ABA pathway to attenuate the inhibitory effect of ABA on rice seed germination via dual strategies.展开更多
The key regulators and regeneration-associated genes involved in axonal regeneration of neurons after injury have not been clarified.In high-throughput sequencing,various factors influence the final sequencing results...The key regulators and regeneration-associated genes involved in axonal regeneration of neurons after injury have not been clarified.In high-throughput sequencing,various factors influence the final sequencing results,including the number and size of cells,the depth of sequencing,and the method of cell separation.There is still a lack of research on the detailed molecular expression profile during the regeneration of dorsal root ganglion neuron axon.In this study,we performed lase r-capture microdissection coupled with RNA sequencing on dorsal root ganglion neurons at 0,3,6,and 12 hours and 1,3,and 7 days after sciatic nerve crush in rats.We identified three stages after dorsal root ganglion injury:early(3-12 hours),pre-regeneration(1 day),and regeneration(3-7 days).Gene expression patterns and related function enrichment res ults showed that one module of genes was highly related to axonal regeneration.We verified the up-regulation of activating transcription factor 3(Atf3),Kruppel like factor 6(Klf6),AT-rich inte raction domain 5A(Arid5α),CAMP responsive element modulator(Crem),and FOS like 1,AP-1 transcription factor Subunit(Fosl1) in dorsal root ganglion neurons after injury.Suppressing these transcription factors(Crem,Arid5o,Fosl1 and Klf6) reduced axonal regrowth in vitro.As the hub transcription factor,Atf3 showed higher expression and activity at the preregeneration and regeneration stages.G protein-coupled estrogen receptor 1(Gper1),inte rleukin 12a(Il12α),estrogen receptor 1(ESR1),and interleukin 6(IL6) may be upstream factors that trigger the activation of Atf3 during the repair of axon injury in the early stage.Our study presents the detailed molecular expression profile during axonal regeneration of dorsal root ganglion neurons after peripheral nerve injury.These findings may provide reference for the clinical screening of molecular targets for the treatment of peripheral nerve injury.展开更多
文摘Objective:To study the correlation of Runt-related transcription factor gene 3 (RUNX3) expression in osteosarcoma tissue with cell proliferation and angiogenesis.Methods: A total of 80 patients with osteosarcoma who were treated in our hospital between February 2014 and February 2017 were collected, and the RUNX3 expression in osteosarcoma tissue and adjacent tissue were detected. According to the RUNX3 expression in tumor tissue, the patients were further divided into high RUNX3 expression group and low RUNX3 expression group, and the proliferation gene and angiogenesis gene expression were compared.Results:RUNX3, KISS-1 and RanBP9 mRNA expression in osteosarcoma tissue were significantly lower than those in adjacent tissue while VCP, Six1, S100A6, IF-1α, MMP-14, bFGF and Ang-2 mRNA expression were significantly higher than those in adjacent tissue;KISS-1 and RanBP9 mRNA expression in osteosarcoma tissue of high RUNX3 expression group were significantly higher than those of low RUNX3 expression group while VCP, Six1, S100A6, IF-1 , MMP-14, bFGF and Ang-2 mRNA expression were significantly lower than those of low RUNX3 expression group.Conclusions:The desease of RUNX3 expression in osteosarcoma tissue is one of the direct causes of increased tumor proliferation activity and strong angiogenesis.
文摘Objective: To investigate the correlation of Runt-related transcription factor 2 (RunX2) with proliferation genes, tumor suppressor genes and angiogenesis molecules in colon cancer lesions. Methods: A total of 90 patients with primary colon cancer were enrolled in colon cancer group, 68 patients with benign colon polyps were enrolled in colon polyps group, the differences in the expression levels of RunX2, proliferation genes, tumor suppressor genes and angiogenesis molecules in the two groups of lesions were compared, and Pearson test was further used to evaluate the correlation of RunX2 expression level with proliferation gene, tumor suppressor gene and angiogenesis molecule expression levels in colon cancer tissues. Results: RunX2 mRNA expression level in the lesions of colon cancer group was higher than that of colon polyps group. Proliferation genes GTPBP4, HOXB7, ZNF331, ADAM17 and HSP60 mRNA expression levels in the lesions of colon cancer group were higher than those of colon polyps group;tumor suppressor genes ATF3, FOXN3, OTUD1 and NDRG2 mRNA expression levels were lower than those of colon polyps group;angiogenesis molecules Musashi 1, NF-κB, RegⅣ and STAT3 mRNA expression levels were higher than those of colon polyps group. RunX2 mRNA expression level in the colon cancer lesions was directly correlated with the expression levels of the above proliferation genes, tumor suppressor genes and angiogenesis molecules. Conclusion: RunX2 expression is abnormally high in colon cancer lesions, the specific expression level is positively correlated with cancer cell proliferation activity and angiogenesis activity, and it is an important molecular target that can lead to the occurrence and development of colon cancer.
基金This research was supported by the University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province,China(UNPYSCT-2018169)the China Postdoctoral Science Foundation Grant(2018 M630333)+1 种基金the National Key R&D Program of China(2017YFD0101900)the earmarked fund for China Agriculture Research System(CARS-23-A-16).
文摘BRI1-EMS-SUPPRESSOR 1(BES1)transcription factor is closely associated with the brassinosteroid(BR)signaling pathway and plays an important role in plant growth and development.SLB3 is a member of BES1 transcription factor family and its expression was previously shown to increase significantly in tomato seedlings under drought stress.In the present study,we used virus-induced gene silencing(VIGS)technology to downregulate SLB3 expression to reveal the function of the SLB3 gene under drought stress further.The downregulated expression of SLB3 weakened the drought tolerance of the plants appeared earlier wilting and higher accumulation of H2 O2 and O2^–·,decreased superoxide dismutase(SOD)activity,and increased proline(PRO)and malondialdehyde(MDA)contents and peroxidase(POD)activity.Quantitative real-time PCR(qRT-PCR)analysis of BR-related genes revealed that the expression of SlCPD,SlDWARF and BIN2-related genes was significantly upregulated in SLB3-silenced seedlings under drought stress,but that the expression of TCH4-related genes was downregulated.These results showed that silencing the SLB3 gene reduced the drought resistance of tomato plants and had an impact on the BR signaling transduction which may be probably responsible for the variation in drought resistance of the tomato plants.
基金National Natural Science Foundation of China(31661143002,81760507,31571709,31771839,31701123 and 31501034)Yunnan Applied Basic Research Projects(2016FA011,2016FB060 and 2016FB040)+1 种基金the National R&D Infrastructure and Facility development Program of China"Fundamental Science Data Sharing Platform(DKA 201712-02-16)the 13th Five-year informatization Plan of Chinese Academy of Sciences(No.XXH13506)。
文摘The B3 transcription factors(TFs)in plants play vital roles in numerous biological processes.Although B3 genes have been broadly identified in many plants,little is known about their potential functions in mediating seed development and material accumulation.Castor bean(Ricinus communis)is a non-edible oilseed crop considered an ideal model system for seed biology research.Here,we identified a total of 61 B3 genes in the castor bean genome,which can be classified into five subfamilies,including ABI3/VP1,HSI,ARF,RAV and REM.The expression profiles revealed that RcABI3/VP1 subfamily genes are significantly up-regulated in the middle and later stages of seed development,indicating that these genes may be associated with the accumulation of storage oils.Furthermore,through yeast one-hybrid and tobacco transient expression assays,we detected that ABI3/VP1 subfamily member RcLEC2 directly regulates the transcription of RcOleosin2,which encodes an oil-body structural protein.This finding suggests that RcLEC2,as a seed-specific TF,may be involved in the regulation of storage materials accumulation.This study provides novel insights into the potential roles and molecular basis of B3 family proteins in seed development and material accumulation.
基金supported by grants from the National Key Research&Development Plan,China (Grant Nos.2021YFD1200201,2022YFD1200502)National Natural Science Foundation of China(31972426,31991182)+3 种基金Key Project of Hubei Hongshan Laboratory(Grant No.2021hszd007)Wuhan Major Project of Key Technologies in Biological Breeding (Grant No.2022021302024852)Fundamental Research Funds for the Central Universities,China (Grant No.2662022YLPY001)International Cooperation Promotion Plan of Shihezi University (Grant No.GJHZ202104)。
文摘High temperature stress is one of the major environmental factors that affect the growth and development of plants. Although WRKY transcription factors play a critical role in stress responses, there are few studies on the regulation of heat stress by WRKY transcription factors,especially in tomato. Here, we identified a group I WRKY transcription factor, SlWRKY3, involved in thermotolerance in tomato. First, SlWRKY3 was induced and upregulated under heat stress. Accordingly, overexpression of SlWRKY3 led to an increase, whereas knock-out of SlWRKY3 resulted in decreased tolerance to heat stress. Overexpression of SlWRKY3 accumulated less reactive oxygen species(ROS), whereas knock-out of SlWRKY3 accumulated more ROS under heat stress. This indicated that SlWRKY3 positively regulates heat stress in tomato. In addition,SlWRKY3 activated the expression of a range of abiotic stress-responsive genes involved in ROS scavenging, such as a SlGRXS1 gene cluster.Further analysis showed that SlWRKY3 can bind to the promoters of the SlGRXS1 gene cluster and activate their expression. Collectively, these results imply that SlWRKY3 is a positive regulator of thermotolerance through direct binding to the promoters of the SlGRXS1 gene cluster and activating their expression and ROS scavenging.
基金supported by the National Training Programs of Innovation and Entrepreneurship for Undergraduates,Science Fund for Distinguished Young Scholars of Jiangsu Province,China(Grant No.BK20200045)the Priority Academic Program Development of Jiangsu Higher Education Institutions Program,China.
文摘We successfully identified a novel and unique OsbZIP transcription factor,OsbZIP09,whose mutants exhibited longer seeds and less severe pre-harvest sprouting than the wild type,but shared similar germination rate as the wild type under normal germination conditions.The expression of OsbZIP09 was induced by abscisic acid(ABA)and declined as the germination process.As a nucleus-localized transcription factor,the conserved binding motif of OsbZIP09 was identified via DNA affinity purification sequencing technique.Further evidences indicated that OsbZIP09 directly enhanced the expression of ABA catabolism gene ABA8ox1,thus reducing ABA accumulation.In addition,OsbZIP09 also directly bound to the promoter of LEA3 gene to inhibit its expression,thus further alleviating the suppressive effect of ABA on seed germination.These results demonstrated that OsbZIP09 likely functions as a brake of the ABA pathway to attenuate the inhibitory effect of ABA on rice seed germination via dual strategies.
基金supported by the National Natural Science Foundation of China,Nos. 31730031 and 32130060the National Major Project of Research and Development,No. 2017YFA0104700the Natural Science Foundation of Jiangsu Province,No. BK20202013 (all to XSG)。
文摘The key regulators and regeneration-associated genes involved in axonal regeneration of neurons after injury have not been clarified.In high-throughput sequencing,various factors influence the final sequencing results,including the number and size of cells,the depth of sequencing,and the method of cell separation.There is still a lack of research on the detailed molecular expression profile during the regeneration of dorsal root ganglion neuron axon.In this study,we performed lase r-capture microdissection coupled with RNA sequencing on dorsal root ganglion neurons at 0,3,6,and 12 hours and 1,3,and 7 days after sciatic nerve crush in rats.We identified three stages after dorsal root ganglion injury:early(3-12 hours),pre-regeneration(1 day),and regeneration(3-7 days).Gene expression patterns and related function enrichment res ults showed that one module of genes was highly related to axonal regeneration.We verified the up-regulation of activating transcription factor 3(Atf3),Kruppel like factor 6(Klf6),AT-rich inte raction domain 5A(Arid5α),CAMP responsive element modulator(Crem),and FOS like 1,AP-1 transcription factor Subunit(Fosl1) in dorsal root ganglion neurons after injury.Suppressing these transcription factors(Crem,Arid5o,Fosl1 and Klf6) reduced axonal regrowth in vitro.As the hub transcription factor,Atf3 showed higher expression and activity at the preregeneration and regeneration stages.G protein-coupled estrogen receptor 1(Gper1),inte rleukin 12a(Il12α),estrogen receptor 1(ESR1),and interleukin 6(IL6) may be upstream factors that trigger the activation of Atf3 during the repair of axon injury in the early stage.Our study presents the detailed molecular expression profile during axonal regeneration of dorsal root ganglion neurons after peripheral nerve injury.These findings may provide reference for the clinical screening of molecular targets for the treatment of peripheral nerve injury.
