(Ca, Mg)-α′-Sialon-AlN-BN powders were synthesized by the carbothermal reduction and nitridation (CRN) method using boron-rich slag, one of the intermediate products from pyrometallurgy separation of pageit, as the ...(Ca, Mg)-α′-Sialon-AlN-BN powders were synthesized by the carbothermal reduction and nitridation (CRN) method using boron-rich slag, one of the intermediate products from pyrometallurgy separation of pageit, as the staring material. The influences of synthesis temperature and holding time on the phase composition and microstructure during the microwave CRN were studied by XRD, SEM and EDS. The comparison between two heating techniques, conventional and microwave heating, on the synthesized powder was presented as well. The experimental results revealed that the phase compositions and microstructures of the synthesized products were greatly affected by the synthesis temperature and holding time. With an increase in the synthesis temperature or holding time, the relative amount of α′-Sialon increased and α′-Sialon became the main crystalline phase at 1400 °C for 6 h. The synthesized products also contained AlN, BN and a small amount of β-SiC. Elongated α′-Sialon grains, short rod AlN grains, aggregate nanoscale BN grains were observed in the synthesized powders. The reaction temperature of microwave heating method was reduced by 80 °C, the reaction time was shortened by 2 h, and more elongated α′-Sialon grains with large aspect ratio were observed.展开更多
Bivalve shell fossils,cemented by authigenic carbonates,are widely spread in the Haima cold seep,Qiongdongnan Basin of the South China Sea(SCS).In this study,we examined an element profile of Gigantidas platifrons in ...Bivalve shell fossils,cemented by authigenic carbonates,are widely spread in the Haima cold seep,Qiongdongnan Basin of the South China Sea(SCS).In this study,we examined an element profile of Gigantidas platifrons in the Haima cold seeps at a water depth of 1381 m.Based on the scanning electron microscope(SEM)analyses,the prismatic layer and nacreous layer were identified,which are characterized by prismatic structure and stratified structure,respectively.In addition,the profile can be subdivided into two parts:altered and unaltered zones.Laser inductively coupled plasma mass spectrometry(LA-ICP-MS)mapping shows that the element concentrations of the altered zones were influenced by the authigenic carbonate rocks,whereas the element concentrations of unaltered zones remain stable.In-situ X-ray diffraction(XRD)analyses show that the mineral constituent of the prismatic layer is mainly composed of aragonite.Along with the growth profile,Mg/Ca ratios of unaltered zones have minor variations,ranging 0.72-0.97 mmol/mol(mean=0.87 mmol/mol),with estimated temperatures of 3.8-4.1℃,indicating that the temperature of the surrounding seawater remains constant and agree with the measured data of 3.9℃which was conducted by a conductivity-temperature-depth system(CTD).The minor variations of Ba/Ca ratios(0.01-0.06 mmol/mol;mean=0.04 mmol/mol)indicate a relatively stabilized salinity of the surrounding seawater.S/Ca ratios show large variations of 0.04-4.15 mmol/mol(mean=1.37 mmol/mol).S/Ca ratios have regular variations which generally correspond to the variations of the Mg/Ca ratios,highlighting that the S/Ca ratios of bivalve shells show the potential to reflect the growth rate of the Gigantides.However,further studies should be carried out on the understanding of the links between the S/Ca ratios and seepage intensity of cold-seep fluids.展开更多
Recent studies revealed that in eutectic to slightly hypereutectic grey irons （CE = 4.3%-4.5%） the presence of austenite dendrites provides an opportunity to improve the cast iron properties, as a high number of eut...Recent studies revealed that in eutectic to slightly hypereutectic grey irons （CE = 4.3%-4.5%） the presence of austenite dendrites provides an opportunity to improve the cast iron properties, as a high number of eutectic cells are ＂reinforced＂ by austenite dendrites. An iron powder addition proved to be important by promoting dendritic austenite in hypereutectic irons, but was accompanied by adverse effect on the characteristics of potential nuclei for graphite. The purpose of the present paper is to investigate the solidification pattern of these irons. Chill wedges with different cooling moduli （CM = 0.11 - 0.43 cm） were poured in resin bonded sand and metal moulds. Relative clear / mottled / total chill measurement criteria were applied. Iron powder additions led to a higher chill tendency, while single inoculation showed the strongest graphiUzing effect. The various double treatments show an intermediate position, but the inoculant added after iron powder appears to be the most effective in reducing base iron chill tendency, for all cooling moduli and chill evaluation parameters. This performance reflects the improved properties of （Mn,X）S polygonal compounds as nucleation sites for graphite, especially in resin bonded sand mould castings. Both austenite and graphite nucleation benefit from a double addition of iron powder ＋ inoculant, with positive effect on the final structure and chill tendency.展开更多
Background: The prevalence of Parkinson’s disease (PD), a chronic and progressive neurodegenerative disorder, is projected to increase twofold by 2030. Leucine-rich repeat kinase 2 (LRRK2) is the most commonly observ...Background: The prevalence of Parkinson’s disease (PD), a chronic and progressive neurodegenerative disorder, is projected to increase twofold by 2030. Leucine-rich repeat kinase 2 (LRRK2) is the most commonly observed gene in both familial and sporadic PD cases. Notably, there is a substantial augmentation in motor activity during both larval and adult stages of zebrafish lacking the lrrk2 gene. Nevertheless, the precise genetic abnormalities accountable for eliciting these phenotypes in zebrafish are yet to be elucidated. Methods: Real-time polymerase chain reaction (PCR) was conducted on zebrafish larvae at 6 days post fertilization (dpf) belonging to both the wild-type and lrk2(-/-) groups. Guide RNA was designed and subsequently employed in the PCR process. Electrophoresis was performed to facilitate identification. Results: The expression of CNTF mRNA was significantly diminished in lrrk2(-/-), in comparison to the wildtype zebrafish larvae. This finding implies that CNTF may have crucial implications in the regulated functioning of lrrk2, which is widely acknowledged as the predominant genetic factor contributing to hereditary PD. The primers for CNTF DNA were meticulously designed, and the electrophoresis results of the PCR product were subsequently presented. The wild type zebrafish embryos were meticulously prepared for micro-injection, and the resulting efficiency identification displayed the presence of the mutant PCR product, which exhibited the presence of several debris. Conclusions: The present study demonstrates the successful generation of CNTF mutant zebrafish using the CRISPR/Cas9 genome editing technique. Further investigations are necessary to deepen our understanding of the exogenous CNTF gene’s functionality.展开更多
Objective:To study the potential therapeutic effect of Yigan Powder on Alzheimer’s disease(AD)comorbid Depressive Disorder with Network Pharmacology.Methods:The active ingredients of Yigan Powder were screened from T...Objective:To study the potential therapeutic effect of Yigan Powder on Alzheimer’s disease(AD)comorbid Depressive Disorder with Network Pharmacology.Methods:The active ingredients of Yigan Powder were screened from Traditional Chinese Medicine Systems Pharmacology database and analysis platform(TCMSP)by ADME parameters,targets are predicted by Swiss Target Prediction database,disease targets are downloaded from GeneCards,OMIM and PharGKB database.Using R to run GO terms enrichment and KEGG pathway enrichment analysis.Protein interaction data is downloaded from the String database.Cytoscape software is used to build network.AutoDock Vina and PyMOL software are used for molecular docking.Results:There are 125 active ingredients in Yigan Powder with 953 predicted targets,85 of predicted targets are related to Alzheimer's Disease comorbid Depressive Disorder,shows highly enriched signaling pathways and biological processes.PPI network shows APP,MAPK1 and STAT3 may be important potential treatment of Alzheimer's Disease comorbid Depressive Disorder.The results of AutoDock Vina docking showed that the active ingredients of Yigan Powder had good binding activity with important receptors.Conclusion:Yigan Powder shows effect on neurotransmitter metabolism,synaptic transmission,neuroinflammation and other aspects in the treatment of Alzheimer's Disease comorbid Depressive Disorder.展开更多
基金Project (2006AA06Z368) supported by High-tech Research and Development Programs of ChinaProject (N100402007) supported by the Fundamental Research Funds for the Central Universities in China
文摘(Ca, Mg)-α′-Sialon-AlN-BN powders were synthesized by the carbothermal reduction and nitridation (CRN) method using boron-rich slag, one of the intermediate products from pyrometallurgy separation of pageit, as the staring material. The influences of synthesis temperature and holding time on the phase composition and microstructure during the microwave CRN were studied by XRD, SEM and EDS. The comparison between two heating techniques, conventional and microwave heating, on the synthesized powder was presented as well. The experimental results revealed that the phase compositions and microstructures of the synthesized products were greatly affected by the synthesis temperature and holding time. With an increase in the synthesis temperature or holding time, the relative amount of α′-Sialon increased and α′-Sialon became the main crystalline phase at 1400 °C for 6 h. The synthesized products also contained AlN, BN and a small amount of β-SiC. Elongated α′-Sialon grains, short rod AlN grains, aggregate nanoscale BN grains were observed in the synthesized powders. The reaction temperature of microwave heating method was reduced by 80 °C, the reaction time was shortened by 2 h, and more elongated α′-Sialon grains with large aspect ratio were observed.
基金Supported by the Key Research and Development Project of Guangdong Province(No.2020B1111510001)the National Natural Science Foundation of China(No.U2244224)+1 种基金the PI Project of Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou)(No.GML2020GD0802)the Guangdong Special Support Team Program(No.2019BT02H594)。
文摘Bivalve shell fossils,cemented by authigenic carbonates,are widely spread in the Haima cold seep,Qiongdongnan Basin of the South China Sea(SCS).In this study,we examined an element profile of Gigantidas platifrons in the Haima cold seeps at a water depth of 1381 m.Based on the scanning electron microscope(SEM)analyses,the prismatic layer and nacreous layer were identified,which are characterized by prismatic structure and stratified structure,respectively.In addition,the profile can be subdivided into two parts:altered and unaltered zones.Laser inductively coupled plasma mass spectrometry(LA-ICP-MS)mapping shows that the element concentrations of the altered zones were influenced by the authigenic carbonate rocks,whereas the element concentrations of unaltered zones remain stable.In-situ X-ray diffraction(XRD)analyses show that the mineral constituent of the prismatic layer is mainly composed of aragonite.Along with the growth profile,Mg/Ca ratios of unaltered zones have minor variations,ranging 0.72-0.97 mmol/mol(mean=0.87 mmol/mol),with estimated temperatures of 3.8-4.1℃,indicating that the temperature of the surrounding seawater remains constant and agree with the measured data of 3.9℃which was conducted by a conductivity-temperature-depth system(CTD).The minor variations of Ba/Ca ratios(0.01-0.06 mmol/mol;mean=0.04 mmol/mol)indicate a relatively stabilized salinity of the surrounding seawater.S/Ca ratios show large variations of 0.04-4.15 mmol/mol(mean=1.37 mmol/mol).S/Ca ratios have regular variations which generally correspond to the variations of the Mg/Ca ratios,highlighting that the S/Ca ratios of bivalve shells show the potential to reflect the growth rate of the Gigantides.However,further studies should be carried out on the understanding of the links between the S/Ca ratios and seepage intensity of cold-seep fluids.
文摘Recent studies revealed that in eutectic to slightly hypereutectic grey irons （CE = 4.3%-4.5%） the presence of austenite dendrites provides an opportunity to improve the cast iron properties, as a high number of eutectic cells are ＂reinforced＂ by austenite dendrites. An iron powder addition proved to be important by promoting dendritic austenite in hypereutectic irons, but was accompanied by adverse effect on the characteristics of potential nuclei for graphite. The purpose of the present paper is to investigate the solidification pattern of these irons. Chill wedges with different cooling moduli （CM = 0.11 - 0.43 cm） were poured in resin bonded sand and metal moulds. Relative clear / mottled / total chill measurement criteria were applied. Iron powder additions led to a higher chill tendency, while single inoculation showed the strongest graphiUzing effect. The various double treatments show an intermediate position, but the inoculant added after iron powder appears to be the most effective in reducing base iron chill tendency, for all cooling moduli and chill evaluation parameters. This performance reflects the improved properties of （Mn,X）S polygonal compounds as nucleation sites for graphite, especially in resin bonded sand mould castings. Both austenite and graphite nucleation benefit from a double addition of iron powder ＋ inoculant, with positive effect on the final structure and chill tendency.
文摘Background: The prevalence of Parkinson’s disease (PD), a chronic and progressive neurodegenerative disorder, is projected to increase twofold by 2030. Leucine-rich repeat kinase 2 (LRRK2) is the most commonly observed gene in both familial and sporadic PD cases. Notably, there is a substantial augmentation in motor activity during both larval and adult stages of zebrafish lacking the lrrk2 gene. Nevertheless, the precise genetic abnormalities accountable for eliciting these phenotypes in zebrafish are yet to be elucidated. Methods: Real-time polymerase chain reaction (PCR) was conducted on zebrafish larvae at 6 days post fertilization (dpf) belonging to both the wild-type and lrk2(-/-) groups. Guide RNA was designed and subsequently employed in the PCR process. Electrophoresis was performed to facilitate identification. Results: The expression of CNTF mRNA was significantly diminished in lrrk2(-/-), in comparison to the wildtype zebrafish larvae. This finding implies that CNTF may have crucial implications in the regulated functioning of lrrk2, which is widely acknowledged as the predominant genetic factor contributing to hereditary PD. The primers for CNTF DNA were meticulously designed, and the electrophoresis results of the PCR product were subsequently presented. The wild type zebrafish embryos were meticulously prepared for micro-injection, and the resulting efficiency identification displayed the presence of the mutant PCR product, which exhibited the presence of several debris. Conclusions: The present study demonstrates the successful generation of CNTF mutant zebrafish using the CRISPR/Cas9 genome editing technique. Further investigations are necessary to deepen our understanding of the exogenous CNTF gene’s functionality.
基金General Program of National Natural Science Foundation(No.81874422)Excellent Inheritance Team of Beijing University of Chinese Medicine-Wang Yongyan Famous Doctor Inheritance Workstation Construction Project(No.1000062720028/010)。
文摘Objective:To study the potential therapeutic effect of Yigan Powder on Alzheimer’s disease(AD)comorbid Depressive Disorder with Network Pharmacology.Methods:The active ingredients of Yigan Powder were screened from Traditional Chinese Medicine Systems Pharmacology database and analysis platform(TCMSP)by ADME parameters,targets are predicted by Swiss Target Prediction database,disease targets are downloaded from GeneCards,OMIM and PharGKB database.Using R to run GO terms enrichment and KEGG pathway enrichment analysis.Protein interaction data is downloaded from the String database.Cytoscape software is used to build network.AutoDock Vina and PyMOL software are used for molecular docking.Results:There are 125 active ingredients in Yigan Powder with 953 predicted targets,85 of predicted targets are related to Alzheimer's Disease comorbid Depressive Disorder,shows highly enriched signaling pathways and biological processes.PPI network shows APP,MAPK1 and STAT3 may be important potential treatment of Alzheimer's Disease comorbid Depressive Disorder.The results of AutoDock Vina docking showed that the active ingredients of Yigan Powder had good binding activity with important receptors.Conclusion:Yigan Powder shows effect on neurotransmitter metabolism,synaptic transmission,neuroinflammation and other aspects in the treatment of Alzheimer's Disease comorbid Depressive Disorder.
