Objective： To observe the effect of acupuncture-moxibustion on TNF-α, sTNFR-Ⅰ and sTNFR-Ⅱ in rats with Crohn＇s disease （CD）. Method： The models of CD rats induced by TNBS were randomized into model group, herb...Objective： To observe the effect of acupuncture-moxibustion on TNF-α, sTNFR-Ⅰ and sTNFR-Ⅱ in rats with Crohn＇s disease （CD）. Method： The models of CD rats induced by TNBS were randomized into model group, herbal cake-partitioned moxibustion group and electroacupuncture group as well as a normal control group. The histopathological changes of colon mucus membrane were observed with HE staining and the contents of TNF-α, sTNFR-Ⅰ and sTNFR-Ⅱ were detected with ELISA method. Results： When compared with the normal group, the TNF-α level in CD rats was substantially elevated and the sTNFR-Ⅰ and sTNFR-Ⅱ showed no marked changes. After herbal cake-partitioned moxibustion and electroacupuncture treatment, the TNF-α level in CD rats was substantially reduced and sTNFR-Ⅰ and sTNFR-Ⅱ showed no marked changes. The inflammatory lesions and abnormal structures of CD rats were significantly improved after herbal cake-partitioned moxibustion and electric stimulation. Conclusion： Herbal cake-partitioned moxibustion and electroacupuncture can both remarkably reduce the TNF-α level in blood serum, and this might be one of the action mechanisms in the treatment of CD.展开更多
基金National Natural Science Foundation of China (30271638)
文摘Objective： To observe the effect of acupuncture-moxibustion on TNF-α, sTNFR-Ⅰ and sTNFR-Ⅱ in rats with Crohn＇s disease （CD）. Method： The models of CD rats induced by TNBS were randomized into model group, herbal cake-partitioned moxibustion group and electroacupuncture group as well as a normal control group. The histopathological changes of colon mucus membrane were observed with HE staining and the contents of TNF-α, sTNFR-Ⅰ and sTNFR-Ⅱ were detected with ELISA method. Results： When compared with the normal group, the TNF-α level in CD rats was substantially elevated and the sTNFR-Ⅰ and sTNFR-Ⅱ showed no marked changes. After herbal cake-partitioned moxibustion and electroacupuncture treatment, the TNF-α level in CD rats was substantially reduced and sTNFR-Ⅰ and sTNFR-Ⅱ showed no marked changes. The inflammatory lesions and abnormal structures of CD rats were significantly improved after herbal cake-partitioned moxibustion and electric stimulation. Conclusion： Herbal cake-partitioned moxibustion and electroacupuncture can both remarkably reduce the TNF-α level in blood serum, and this might be one of the action mechanisms in the treatment of CD.
