The Fluorescence Immunochromatographic Strips for Natamycin and Its Application in Food Safety Detection

A fluorescence immunochromatographic strip was developed in this study for natamycin detection in food. The results showed that the best amount of labeled antibody was 10 μg, for every 50 μl of fluorescent microspheres with a 2.5%(w/v) concentration. This labeled antibody was diluted for 10 times, and the diluted solution was dispensed into conjugate pad at the amount of 3 μl/cm. The concentrations of natamycin labeled BSA for test line and goat anti-mouse IgG for control line were 2.0 and 1 mg/ml, respectively, which performed best. With the best conditions, the limit of detection was 1 ng/ml, the linearity ranged from 2 to 100 ng/ml, the recovery was about 80% to 120%, and the CV was below 23%.

Food Safety Detection Methods Applied to National Special Rectification of Product Quality and Food Safety

Afour-month period of national special rectification for product quality and food safety officially started on August 25, and was focused on eight fields, including those of agricultural products and processed foods.……

Real-Time Safety Helmet Detection Using Yolov5 at Construction Sites

The construction industry has always remained the economic and social backbone of any country in the world where occupational health and safety(OHS)is of prime importance.Like in other developing countries,this industry pays very little,rather negligible attention to OHS practices in Pakistan,resulting in the occurrence of a wide variety of accidents,mishaps,and near-misses every year.One of the major causes of such mishaps is the non-wearing of safety helmets(hard hats)at construction sites where falling objects from a height are unavoid-able.In most cases,this leads to serious brain injuries in people present at the site in general and the workers in particular.It is one of the leading causes of human fatalities at construction sites.In the United States,the Occupational Safety and Health Administration(OSHA)requires construction companies through safety laws to ensure the use of well-defined personal protective equipment(PPE).It has long been a problem to ensure the use of PPE because round-the-clock human monitoring is not possible.However,such monitoring through technological aids or automated tools is very much possible.The present study describes a systema-tic strategy based on deep learning(DL)models built on the You-Only-Look-Once(YOLOV5)architecture that could be used for monitoring workers’hard hats in real-time.It can indicate whether a worker is wearing a hat or not.The proposed system usesfive different models of the YOLOV5,namely YOLOV5n,YOLOv5s,YOLOv5 m,YOLOv5l,and YOLOv5x for object detection with the support of PyTorch,involving 7063 images.The results of the study show that among the DL models,the YOLOV5x has a high performance of 95.8%in terms of the mAP,while the YOLOV5n has the fastest detection speed of 70.4 frames per second(FPS).The proposed model can be successfully used in practice to recognize the hard hat worn by a worker.

Real-time Safety Helmet-wearing Detection Based on Improved YOLOv5

Safety helmet-wearing detection is an essential part of the intelligentmonitoring system. To improve the speed and accuracy of detection, especiallysmall targets and occluded objects, it presents a novel and efficient detectormodel. The underlying core algorithm of this model adopts the YOLOv5 (YouOnly Look Once version 5) network with the best comprehensive detection performance. It is improved by adding an attention mechanism, a CIoU (CompleteIntersection Over Union) Loss function, and the Mish activation function. First,it applies the attention mechanism in the feature extraction. The network can learnthe weight of each channel independently and enhance the information dissemination between features. Second, it adopts CIoU loss function to achieve accuratebounding box regression. Third, it utilizes Mish activation function to improvedetection accuracy and generalization ability. It builds a safety helmet-wearingdetection data set containing more than 10,000 images collected from the Internetfor preprocessing. On the self-made helmet wearing test data set, the averageaccuracy of the helmet detection of the proposed algorithm is 96.7%, which is1.9% higher than that of the YOLOv5 algorithm. It meets the accuracy requirements of the helmet-wearing detection under construction scenarios.

Application of high-density resistivity method to evaluate safety around Minyan Tunnel,Helong City

By determining the distribution and extent of geological structures surrounding the Mingyan Tunnel,Xicheng Town,Helong City,Jilin Province,we can evaluate the stability of the rock mass and assess potential hazards during tunnel construction.We use the high-density resistivity method to analyze the subsurface structure of the study area.Conductive anomalies are likely to represent joint and fissure systems within strongly weathered host rocks,and the bedrock surrounding the tunnel is relatively stable and does not contain well-developed faults.High-density resistivity analysis can provide valuable information in the context of tunnel engineering and safety.

Application of high-density resistivity method for assessing construction safety of Shimodong tunnel in Helong City of Jilin Province

Some unfavorable geological conditions can affect the construction of tunnels.In order to evaluate the damage degree of tunnel construction and determine the surrounding rock grade and stability of the tunnel,the authors used high-density resistivity method to detect the surrounding rocks of Shimodong tunnel in Xicheng Town of Helong City.The underground resistivity structures of the entrance,exit and middle parts of the tunnel are obtained.Through analysis,it is found that there are no bedrock faults near the tunnel,although some joints and fissures are developed in some locations,which are characterized by low-resistivity anomalies.The tunnel structures are stable overall,favorable for safe and efficient construction.The study also proves the good application effect of the high-density resistivity method in tunnel safety detection.

Algorithm of Helmet Wearing Detection Based on AT-YOLO Deep Mode

The existing safety helmet detection methods are mainly based on one-stage object detection algorithms with high detection speed to reach the real-time detection requirements,but they can’t accurately detect small objects and objects with obstructions.Therefore,we propose a helmet detection algorithm based on the attention mechanism(AT-YOLO).First of all,a channel attention module is added to the YOLOv3 backbone network,which can adaptively calibrate the channel features of the direction to improve the feature utilization,and a spatial attention module is added to the neck of the YOLOv3 network to capture the correlation between any positions in the feature map so that to increase the receptive field of the network.Secondly,we use DIoU(Distance Intersection over Union)bounding box regression loss function,it not only improving the measurement of bounding box regression loss but also increases the normalized distance loss between the prediction boxes and the target boxes,which makes the network more accurate in detecting small objects and faster in convergence.Finally,we explore the training strategy of the network model,which improves network performance without increasing the inference cost.Experiments show that the mAP of the proposed method reaches 96.5%,and the detection speed can reach 27 fps.Compared with other existing methods,it has better performance in detection accuracy and speed.