Hypoglycemic Effect of Radix Et Rhizoma Salviae Miltiorrhizae,Guixi Green Tea and Their Compatibility on Mice

[Objectives]To explore the hypoglycemic effect of Radix Et Rhizoma Salviae Miltiorrhizae,Guixi green tea and their compatibility on mice,so as to provide more options for the adjuvant treatment of diabetes and improve the comprehensive utilization value of Guixi green tea.[Methods]After being fed adaptively for 7 d,60 SPF-grade male mice were intraperitoneally injected with alloxouracil(180 mg/kg)to induce hyperglycemia model,from which 35 mice with a successful model were selected.They were randomly divided into 5 groups(Danshen group,green tea group,compatible group,positive control group and blank control group),with 7 animals in each group.The mice in all groups were administered according to the dose of 200 mg/kg once a day for 15 d.After the last administration,their body weight was measured on the 15 th day after fasting for 12 h,and their fasting blood glucose was measured by tail clipping.[Results]After modeling,the mice in each group showed typical hyperglycemia symptoms,namely obvious polydipsia,polyphagia,polyuria and weight loss.Compared with the blank control group,Danshen group and green tea group had obvious hypoglycemic effect(P<0.05),and compatible group had significant hypoglycemic effect(P<0.01).Compared with the blank control group,the weight loss of diabetic mice could also be inhibited in green tea group and compatible group(P<0.05).[Conclusions]Danshen,Guixi green tea and their compatibility had the effect of lowering blood sugar,among which the compatible group had the most obvious effect.

自制丹参灵芝三七降脂茶的研究

目的:对丹参灵芝三七降脂茶进行配方、功能、安全等科学研究。方法:对配方进行分析,通过动物实验来评价本品的功效和安全性。结果:本品组方经典、科学、合理,动物实验证明本品具有较好的降脂功效;安全性实验表明本产品安全无毒。结论:本品配方科学合理,降血脂功效明显。

小剂量阿司匹林联合复方丹参滴丸治疗2型糖尿病的效果观察

目的:探讨小剂量阿司匹林联合复方丹参滴丸治疗2型糖尿病的效果。方法:筛选笔者所在医院2017年5月-2018年5月收治2型糖尿病患者110例,按数字表法随机分为对照组和观察组,每组55例。两组予常规干预措施。对照组给予小剂量阿司匹林肠溶片,100 mg/d。观察组在对照组基础上口服复方丹参滴丸,10丸/次,3次/d。两组连续治疗8周。比较两组血糖相关指标水平、血脂水平,分析两组临床疗效。结果:治疗后,观察组患者空腹血糖(FBG)、餐后2 h血糖(PBG)、空腹C肽(FCP)、餐后2 h C肽(PCP)、糖化血红蛋白(HbA1c)水平明显低于对照组(P<0.01)。观察组总有效率为96.36%,显著高于对照组的81.82%(字2=4.583,P=0.032)。治疗后,观察组患者总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)水平显著低于对照组,高密度脂蛋白(HDL)明显高于对照组(P<0.01)。结论:在常规治疗基础上,小剂量阿司匹林联合复方丹参滴丸治疗2型糖尿病可进一步改善患者的糖脂水平,提高临床疗效。

丹参血脂茶质量标准研究

[目的]建立丹参血脂茶中决明子、枳壳定性鉴别及测定丹参酮ⅡA含量方法。[方法]采用TLC对丹参血脂茶中的主要药物决明子、枳壳进行鉴别和高效液相色谱法测定丹参酮ⅡA的含量。[结果]TLC色谱中均能检出决明子、枳壳,专属性强,重现性好;在流动相:甲醇-水(78:22),检测波长270nm,柱温30℃,体积流量1.0m L/min的条件下使用高效液相色谱法对供试品进测定丹参酮ⅡA含量,结果准确可靠,分离完全,重复性好。[结论]TLC与高效液相色谱法简便、专属性强、重现性好,结果稳定,可有效控制丹参血脂茶的质量。

UPLC-Q-TOF/MS分析孟河医派特色猪心血丹参炮制前后化学成分的变化

目的:分析猪心血丹参炮制前后的化学成分变化情况,为深入阐明该饮片的炮制机制提供科学依据。方法:采用超高效液相色谱-四级杆-飞行时间质谱联用技术(UPLC-Q-TOF/MS),色谱条件为ACQUITY UPLC~ C_(18)色谱柱(2.1 mm×100 mm,1.7μm),流动相0.1%甲酸水溶液(A)-0.1%甲酸乙腈溶液(B)梯度洗脱(0~1 min,8%B;1~1.5 min,8%~20%B;1.5~4 min,20%B;4~5 min,20%~60%B;5~9 min,60%~70%B;9~10 min,70%~95%B;10~13 min,95%B),柱温40℃,流速0.3 m L·min^(-1);质谱分析使用电喷雾离子源(ESI),分别在正、负离子模式下扫描,扫描范围均为m/z 50~1 200,采用对照品比对、质谱数据、数据库匹配和文献参照对各离子峰进行归属,通过比较猪心血丹参炮制前后离子峰数目和峰面积,研究其化学成分变化。结果:猪心血丹参炮制前后共鉴别出59个成分,未发现有新的成分产生。猪心血炮制丹参后,共有25种化学成分峰面积发生显着变化,其中丹参水溶性成分原儿茶醛,丹酚酸C,F,G和脂溶性成分丹参醛,丹参二醇A,丹参酮Ⅰ和氨基酸类成分L-苯丙氨酸峰面积显著增高。结论:猪心血丹参炮制前后化学成分的含量变化显著,其中丹参部分水溶性、脂溶性和氨基酸类成分产生了量变,推测这可能与猪心血促进丹参治疗脑缺血的作用有关。