Rice bacterial leaf brown spot disease caused by Pseudomonas syringae pv.syringae(Pss)is a major disease on rice.In recent years,Pss has emerged worldwide,seriously affecting rice production.It is very important to es...Rice bacterial leaf brown spot disease caused by Pseudomonas syringae pv.syringae(Pss)is a major disease on rice.In recent years,Pss has emerged worldwide,seriously affecting rice production.It is very important to establish a rapid detection method of Pss for the diagnosis and prevention of this disease.In order to robust and accurately diagnose the rice bacterial leaf brown spot disease in the field and laboratory,an assay system for the Pss was developed in this study,and the specific sequence of hrcN was used as the target,based on loop-mediated isothermal amplification(LAMP).The best detection system was MgSO 48 mmol·L^(-1),Bst DNA polymerase 8 U,dNTP 1.4 mmol·L^(-1),the ratio of internal and outer primers was 2:1,the reaction temperature was 63℃,the reaction time was 45 min,and the lowest sensitivity was 104 CFU·mL^(-1).This results provided an accurate and robust method for laboratory and field diagnosis of bacterial leaf brown spot disease of rice.展开更多
[Objective] To develop a rapid and visualized detection method using loop-mediated isothermal amplification (LAMP), improve the detection rate of canine parvovirus (CPV) and reduce the testing cost. [ Method] Acco...[Objective] To develop a rapid and visualized detection method using loop-mediated isothermal amplification (LAMP), improve the detection rate of canine parvovirus (CPV) and reduce the testing cost. [ Method] According to the conserved regions of the VP2 gene of CPV, six primers were designed to amplify the special DNA sequences by LAMP. In addition, the reaction conditions of LAMP were optimized, and the sensitivity, specificity, repeatability and stability were verified. [ Result] The optimal reaction time of the LAMP method for CPVwas 60 min. The products obtained by LAMP had high specificity without cross-reaction with other generic viruses. The sensitivity of the LAMP was 100 times higher than that of PCR. [ Conclusion] The LAMP method for detecting CPV has high practical value. It has many advantages such as high specificity, high sensitivity, simple operation, low cost and rapid analysis, and it does not require special equipment. Therefore, this method is more suitable for the detection of CPV.展开更多
Microcapsules containing the aqueous solution of Azur B of a water soluble dye were prepared with the melting dispersion cooling method and applied to the amplification detector of plant DNA. Paraffin wax with melting...Microcapsules containing the aqueous solution of Azur B of a water soluble dye were prepared with the melting dispersion cooling method and applied to the amplification detector of plant DNA. Paraffin wax with melting temperature of 75°C was used as the shell material. In the experiment, the aqueous solution (W) of Azur B as the core material was dispersed in the melted paraffin wax (O) to form the (W/O) emulsion and then, the (W/O) emulsion was dispersed in the silicon oil (O’) as the continuous phase to form the (W/O)/O’ emulsion at 85°C. After formation of the (W/O)/O’ emulsion, the microcapsules were prepared by cooling the (W/O)/O’ emulsion to 50°C. The microcapsules were prepared by changing the concentration of oil soluble surfactant in the (W/O) emulsion and the volume of the (W/O) emulsion in the (W/O)/O’ emulsion. The microencapsulation efficiency increased with the concentration of oil soluble surfactant and finally became 100% under the optimum conditions. Furthermore, the microcapsules were melted down at temperature of 85°C to reveal the sharp thermal responsibility and to release the aqueous solution of Azur B. As a result, it was found that the microcapsules were able to be applied to the amplification detector of plant DNA by utilizing the reaction between DNA and Azur B.展开更多
We report on the auditory Hopf amplification contributed by the electrical energy of the hair cell during its bundle deflecting. An energy method to calculate the active force is adopted according to the electrical en...We report on the auditory Hopf amplification contributed by the electrical energy of the hair cell during its bundle deflecting. An energy method to calculate the active force is adopted according to the electrical energy consumption of the hair cell. After some experimental data was analyzed and simulated, we find that the electrical energy determines the value of the active force and enlarges the mechanical response of the hair bundle. This amplification is controlled by the cell voltage and makes the sensor a Hopf vibrator with hearing nonlinear characteristics. A velocity-dependent active force derived previously from the force-gating channel operation strongly reinforces our conclusion.展开更多
Pseudomonas aeruginosa(PA)is an opportunistic pathogen of humans and animals and a common source of nosocomial infections especially of the respiratory tract.Pseudomonas aeruginosa is also a major bacterial disease of...Pseudomonas aeruginosa(PA)is an opportunistic pathogen of humans and animals and a common source of nosocomial infections especially of the respiratory tract.Pseudomonas aeruginosa is also a major bacterial disease of poultry and in particular,eggs and newly hatched chicks.In this study,we developed a simple,accurate and rapid molecular detection method using cross priming amplification(CPA)with a nucleic acid test strip to detect P.aeruginosa.The assay efficiently amplified the target gene within 45 min at 62℃only using a simple water bath.The detection limit of the method was 1.18x 102 copiesμL^-1 for plasmid DNA and 4.4 CFU mL^-1 for bacteria in pure culture,and was 100 times more sensitive than conventional PCR.We screened 83 clinical samples from yellow-feather broiler breeder chickens and hospitalized/treated dogs and cats using CPA,PCR and traditional culture methods.The positive sample ratios were 15.3%(13/83)by CPA,13.3%(11/83)by PCR and 12.1%(10/83)by the culture method.The established CPA method has significant advantages for detecting P.aeruginosa.The method is easy to use and possesses high specificity and sensitivity without the requirements of complicated experimental equipment.The PA-CPA assay is especially fit for outdoor and primary medical units and is an ideal system for the rapid detection and monitoring of P.aeruginosa.展开更多
As the continuation study on amplification of in-plane seismic ground motion by underground group cavities in layered half-space, this study extends to the case of poroelastic half-space with dry poroelastic and satur...As the continuation study on amplification of in-plane seismic ground motion by underground group cavities in layered half-space, this study extends to the case of poroelastic half-space with dry poroelastic and saturated poroelastic soil layers. The influence of poroelastic layers on the amplification of seismic ground motion is studied both in frequency domain and time domain using indirect boundary element method (IBEM). It is shown that for the example of a saturated poroelastic site in Tianjin under the excitation of Taft wave and E1 Centro wave, the amplification of seismic ground motion in poroelastic case is slightly smaller than that in the elastic case, and the amplification of PGA (peak ground acceleration) and its PRS (peak response spectrum).. can be increased up to 38.8% and 64.6%; the predominant period of response spectra in poroelastic case becomes shorter to some extent compared with that in the elastic case. It is suggested that the effect of underground group cavities in poroelastic half-space on design seismic ground motion should be considered.展开更多
Amplification of in-plane seismic ground motion by underground group cavities in layered half-space is studied both in frequency domain and time domain by using indirect boundary element method (IBEM), and the effec...Amplification of in-plane seismic ground motion by underground group cavities in layered half-space is studied both in frequency domain and time domain by using indirect boundary element method (IBEM), and the effect of cavity interval and spectrum of incident waves on the amplification are studied by numerical examples. It is shown that there may be large interaction between cavities, and group cavities with certain intervals may have significant amplification to seismic ground motion. The amplification of PGA (peak ground acceleration) and its PRS (peak response spectrum) can be increased up to 45.2% and 84.4%, for an example site in Tianjin, under the excitation of Taft wave and E1 Centro wave; and group cavities may also affect the spectra of the seismic ground motion. It is suggested that the effect of underground group cavities on design seismic ground motion should be considered.展开更多
The growing use of underground structures,specifically to facilitate urban transportation,highlights the need to scrutinize the effects of such spaces on the seismic ground response as well as the surrounding building...The growing use of underground structures,specifically to facilitate urban transportation,highlights the need to scrutinize the effects of such spaces on the seismic ground response as well as the surrounding buildings.In this regard,the seismic ground amplification variations in the vicinity of single and twin box-shaped tunnels subjected to SV waves have been investigated by the finite difference method.To evaluate the effects,generalizable dimensionless diagrams based on the results of parametric numerical analysis considering factors such as variations in the tunnels′depth,the distances between the tunnels,tunnel lining flexibility,and input wave frequency,have been presented.In addition,to assess the effects of underground box-shaped tunnels on the response spectrum of the ground surface,seven selected accelerograms have been matched based on a specific design spectrum for the stiff soil condition of Eurocode 8(CEN,2006).The results underline the significant amplification effect of the box-shaped tunnels on the ground motions,specifically in the case of horizontal twin tunnels,which should be given more attention in current seismic design practices for surface structures.展开更多
基金Supported by the Natural Science Foundation of Heilongjiang Province(Topic C2017032)Heilongjiang Province Applied Technology Research and Development Program(Topic GA19B104)the National Key Research and Development Program(Topic 2018YFD0300105)。
文摘Rice bacterial leaf brown spot disease caused by Pseudomonas syringae pv.syringae(Pss)is a major disease on rice.In recent years,Pss has emerged worldwide,seriously affecting rice production.It is very important to establish a rapid detection method of Pss for the diagnosis and prevention of this disease.In order to robust and accurately diagnose the rice bacterial leaf brown spot disease in the field and laboratory,an assay system for the Pss was developed in this study,and the specific sequence of hrcN was used as the target,based on loop-mediated isothermal amplification(LAMP).The best detection system was MgSO 48 mmol·L^(-1),Bst DNA polymerase 8 U,dNTP 1.4 mmol·L^(-1),the ratio of internal and outer primers was 2:1,the reaction temperature was 63℃,the reaction time was 45 min,and the lowest sensitivity was 104 CFU·mL^(-1).This results provided an accurate and robust method for laboratory and field diagnosis of bacterial leaf brown spot disease of rice.
基金supported by Independent Innovation Specific Projects of Shandong Province ( 2008ZHZX1A1103)
文摘[Objective] To develop a rapid and visualized detection method using loop-mediated isothermal amplification (LAMP), improve the detection rate of canine parvovirus (CPV) and reduce the testing cost. [ Method] According to the conserved regions of the VP2 gene of CPV, six primers were designed to amplify the special DNA sequences by LAMP. In addition, the reaction conditions of LAMP were optimized, and the sensitivity, specificity, repeatability and stability were verified. [ Result] The optimal reaction time of the LAMP method for CPVwas 60 min. The products obtained by LAMP had high specificity without cross-reaction with other generic viruses. The sensitivity of the LAMP was 100 times higher than that of PCR. [ Conclusion] The LAMP method for detecting CPV has high practical value. It has many advantages such as high specificity, high sensitivity, simple operation, low cost and rapid analysis, and it does not require special equipment. Therefore, this method is more suitable for the detection of CPV.
文摘Microcapsules containing the aqueous solution of Azur B of a water soluble dye were prepared with the melting dispersion cooling method and applied to the amplification detector of plant DNA. Paraffin wax with melting temperature of 75°C was used as the shell material. In the experiment, the aqueous solution (W) of Azur B as the core material was dispersed in the melted paraffin wax (O) to form the (W/O) emulsion and then, the (W/O) emulsion was dispersed in the silicon oil (O’) as the continuous phase to form the (W/O)/O’ emulsion at 85°C. After formation of the (W/O)/O’ emulsion, the microcapsules were prepared by cooling the (W/O)/O’ emulsion to 50°C. The microcapsules were prepared by changing the concentration of oil soluble surfactant in the (W/O) emulsion and the volume of the (W/O) emulsion in the (W/O)/O’ emulsion. The microencapsulation efficiency increased with the concentration of oil soluble surfactant and finally became 100% under the optimum conditions. Furthermore, the microcapsules were melted down at temperature of 85°C to reveal the sharp thermal responsibility and to release the aqueous solution of Azur B. As a result, it was found that the microcapsules were able to be applied to the amplification detector of plant DNA by utilizing the reaction between DNA and Azur B.
基金Supported by the National Natural Science Foundation of China under Grant Nos 11374118 and 90820001the Science Foundation of Hubei Province under Grant No 2013CFB289
文摘We report on the auditory Hopf amplification contributed by the electrical energy of the hair cell during its bundle deflecting. An energy method to calculate the active force is adopted according to the electrical energy consumption of the hair cell. After some experimental data was analyzed and simulated, we find that the electrical energy determines the value of the active force and enlarges the mechanical response of the hair bundle. This amplification is controlled by the cell voltage and makes the sensor a Hopf vibrator with hearing nonlinear characteristics. A velocity-dependent active force derived previously from the force-gating channel operation strongly reinforces our conclusion.
基金Supported by the Guangdong Key S&T Program(2019B020217002)from the Department of Science and Technology of Guangdong Province,China,the Guangdong Poultry Industry Technology System,China(2019KJ128)the earmarked fund for China Agriculture Research System(CARS-41-G16).
文摘Pseudomonas aeruginosa(PA)is an opportunistic pathogen of humans and animals and a common source of nosocomial infections especially of the respiratory tract.Pseudomonas aeruginosa is also a major bacterial disease of poultry and in particular,eggs and newly hatched chicks.In this study,we developed a simple,accurate and rapid molecular detection method using cross priming amplification(CPA)with a nucleic acid test strip to detect P.aeruginosa.The assay efficiently amplified the target gene within 45 min at 62℃only using a simple water bath.The detection limit of the method was 1.18x 102 copiesμL^-1 for plasmid DNA and 4.4 CFU mL^-1 for bacteria in pure culture,and was 100 times more sensitive than conventional PCR.We screened 83 clinical samples from yellow-feather broiler breeder chickens and hospitalized/treated dogs and cats using CPA,PCR and traditional culture methods.The positive sample ratios were 15.3%(13/83)by CPA,13.3%(11/83)by PCR and 12.1%(10/83)by the culture method.The established CPA method has significant advantages for detecting P.aeruginosa.The method is easy to use and possesses high specificity and sensitivity without the requirements of complicated experimental equipment.The PA-CPA assay is especially fit for outdoor and primary medical units and is an ideal system for the rapid detection and monitoring of P.aeruginosa.
基金supported by National Natural Science Foundation of China under grant No. 50978183Key Project for Applied Basic Research of Tianjin Municipality under Grant No. 12JCZDJC29000
文摘As the continuation study on amplification of in-plane seismic ground motion by underground group cavities in layered half-space, this study extends to the case of poroelastic half-space with dry poroelastic and saturated poroelastic soil layers. The influence of poroelastic layers on the amplification of seismic ground motion is studied both in frequency domain and time domain using indirect boundary element method (IBEM). It is shown that for the example of a saturated poroelastic site in Tianjin under the excitation of Taft wave and E1 Centro wave, the amplification of seismic ground motion in poroelastic case is slightly smaller than that in the elastic case, and the amplification of PGA (peak ground acceleration) and its PRS (peak response spectrum).. can be increased up to 38.8% and 64.6%; the predominant period of response spectra in poroelastic case becomes shorter to some extent compared with that in the elastic case. It is suggested that the effect of underground group cavities in poroelastic half-space on design seismic ground motion should be considered.
基金supported by National Natural Science Foundation of China under grant No. 50978183Tianjin Key Project for Applied Basic Research under grant No. 12JCZDJC29000
文摘Amplification of in-plane seismic ground motion by underground group cavities in layered half-space is studied both in frequency domain and time domain by using indirect boundary element method (IBEM), and the effect of cavity interval and spectrum of incident waves on the amplification are studied by numerical examples. It is shown that there may be large interaction between cavities, and group cavities with certain intervals may have significant amplification to seismic ground motion. The amplification of PGA (peak ground acceleration) and its PRS (peak response spectrum) can be increased up to 45.2% and 84.4%, for an example site in Tianjin, under the excitation of Taft wave and E1 Centro wave; and group cavities may also affect the spectra of the seismic ground motion. It is suggested that the effect of underground group cavities on design seismic ground motion should be considered.
文摘The growing use of underground structures,specifically to facilitate urban transportation,highlights the need to scrutinize the effects of such spaces on the seismic ground response as well as the surrounding buildings.In this regard,the seismic ground amplification variations in the vicinity of single and twin box-shaped tunnels subjected to SV waves have been investigated by the finite difference method.To evaluate the effects,generalizable dimensionless diagrams based on the results of parametric numerical analysis considering factors such as variations in the tunnels′depth,the distances between the tunnels,tunnel lining flexibility,and input wave frequency,have been presented.In addition,to assess the effects of underground box-shaped tunnels on the response spectrum of the ground surface,seven selected accelerograms have been matched based on a specific design spectrum for the stiff soil condition of Eurocode 8(CEN,2006).The results underline the significant amplification effect of the box-shaped tunnels on the ground motions,specifically in the case of horizontal twin tunnels,which should be given more attention in current seismic design practices for surface structures.