A prototype of a miniaturized cell lysis device developed using a concave spherical transducer is capable of lysing bacteria without added chemical denaturants, enzymes or microparticles and is capable of efficient...A prototype of a miniaturized cell lysis device developed using a concave spherical transducer is capable of lysing bacteria without added chemical denaturants, enzymes or microparticles and is capable of efficiently lysing yeast without any mechanical or enzymatic pretreatment. The device is designed for miniature bio analysis systems where cell lysing is needed to obtain intracellular materials for further analysis such as DNA identification. The device lysis efficiency was evaluated using viable cell counts and microscopy. Additionally, the device efficiency was compared with that of traditional chemical cell lysis methods using standard molecular biological techniques such as agarose gels and ultraviolet (UV) spectroscopy. The results indicate that efficient bacteria and cell disruption can be achieved through a low voltage driven and spherically focused high frequency ultrasonic device.展开更多
基金Supported by the National Science Foundation for Outstanding Young Scientists of China(No.3982 5 10 8)
文摘A prototype of a miniaturized cell lysis device developed using a concave spherical transducer is capable of lysing bacteria without added chemical denaturants, enzymes or microparticles and is capable of efficiently lysing yeast without any mechanical or enzymatic pretreatment. The device is designed for miniature bio analysis systems where cell lysing is needed to obtain intracellular materials for further analysis such as DNA identification. The device lysis efficiency was evaluated using viable cell counts and microscopy. Additionally, the device efficiency was compared with that of traditional chemical cell lysis methods using standard molecular biological techniques such as agarose gels and ultraviolet (UV) spectroscopy. The results indicate that efficient bacteria and cell disruption can be achieved through a low voltage driven and spherically focused high frequency ultrasonic device.
