In vitro screening of traditionally used medicinal plants in China against Enteroviruses

AIM： To search for new antiviral agents from traditional Chinese medicine, specifically anti-enterovirosuses agents. METHODS： The aqueous extracts （AE） of more than 100 traditionally used medicinal plants in China were evaluated for their in vitro anti-Coxsackie virus B3 activities with a MTT-based colorimetric assay. RESULTS： The test for AE of 16 plants exhibited anti- Coxsackie virus B3 activities at different magnitudes of potency. They can inhibit three steps （inactivation, adsorption and replication） during the infection. Among the 16 plants, Sargentodoxa cuneata （Oliv.） Rehd. et Wils., Sophora tonkinensis Gapnep., Paeonia veitchii Lynch, Spatholobus suberectus Dunn. and Cyrtorniurn fortunei J, sm. also have activity against other enterovirus, including Coxsackie virus 135, Polio virus I, Echo virus 9 and Echo virus 29. Cell cytotoxic assay demonstrated that all tested AE had CC50 values higher than their EC50 values. CONCLUSION： The sixteen traditionally used medicinal plants in China possessed antMral activity, and some of them merit further investigations.

盆腔炎颗粒的抗炎镇痛作用

目的观察盆腔炎颗粒的抗炎镇痛作用。方法采用小鼠耳肿胀、大鼠足跖肿胀和棉球肉芽肿模型观察盆腔炎颗粒的抗炎作用;采用小鼠扭体反应和光热致痛观察盆腔炎颗粒的镇痛作用。结果盆腔炎颗粒明显抑制小鼠耳肿胀、大鼠角叉菜胶足肿胀和棉球肉芽肿;延长小鼠扭体潜伏期,减少扭体次数和提高光热致痛率。结论盆腔炎颗粒口服给药有显著抗炎和镇痛作用。