Flow cytometry(FCM)is a powerful technique for single-bacteria analysis via simultaneous light-scattering and fluorescence measurements.By offering high-throughput,quantitative,and multiparameter analysis at the singl...Flow cytometry(FCM)is a powerful technique for single-bacteria analysis via simultaneous light-scattering and fluorescence measurements.By offering high-throughput,quantitative,and multiparameter analysis at the single-cell level,FCM has gained an increased popularity in microbiological research,food safety monitoring,water quality control,and clinical diagnosis.Here we will review the recent applications of flow cytometry in areas such as(1)total bacterial cell count,(2)bacterial viability analysis,(3)specific bacterial detection and identification,(4)characterization of physiological changes under environmental perturbations,and(5)biological function studies.Nevertheless,despite these widespread applications,challenges still remain for the detection of small sizes of bacteria and biochemical features that cannot be brightly stained via fluorescence.Recent improvement in FCM instrumentation will be discussed,and particularly the development of high sensitivity flow cytometry for advanced analysis of single bacterial cells will be highlighted.展开更多
For the numerical simulation of time harmonic acoustic scattering in a complex geometry,in presence of an arbitrary mean flow,the main difficulty is the coexistence and the coupling of two very different phenomena:aco...For the numerical simulation of time harmonic acoustic scattering in a complex geometry,in presence of an arbitrary mean flow,the main difficulty is the coexistence and the coupling of two very different phenomena:acoustic propagation and convection of vortices.We consider a linearized formulation coupling an augmented Galbrun equation(for the perturbation of displacement)with a time harmonic convection equation(for the vortices).We first establish the well-posedness of this time harmonic convection equation in the appropriatemathematical framework.Then the complete problem,with Perfectly Matched Layers at the artificial boundaries,is proved to be coercive+compact,and a hybrid numerical method for the solution is proposed,coupling finite elements for the Galbrun equation and a Discontinuous Galerkin scheme for the convection equation.Finally a 2D numerical result shows the efficiency of the method.展开更多
基金the National Key Basic Research Program of China(2013CB933703)the National Natural Science Foundation of China(91313302,21105082,21225523,21472158,21027010,21521004)the Program for Changjiang Scholars and Innovative Research Team in University(IRT13036)
文摘Flow cytometry(FCM)is a powerful technique for single-bacteria analysis via simultaneous light-scattering and fluorescence measurements.By offering high-throughput,quantitative,and multiparameter analysis at the single-cell level,FCM has gained an increased popularity in microbiological research,food safety monitoring,water quality control,and clinical diagnosis.Here we will review the recent applications of flow cytometry in areas such as(1)total bacterial cell count,(2)bacterial viability analysis,(3)specific bacterial detection and identification,(4)characterization of physiological changes under environmental perturbations,and(5)biological function studies.Nevertheless,despite these widespread applications,challenges still remain for the detection of small sizes of bacteria and biochemical features that cannot be brightly stained via fluorescence.Recent improvement in FCM instrumentation will be discussed,and particularly the development of high sensitivity flow cytometry for advanced analysis of single bacterial cells will be highlighted.
文摘For the numerical simulation of time harmonic acoustic scattering in a complex geometry,in presence of an arbitrary mean flow,the main difficulty is the coexistence and the coupling of two very different phenomena:acoustic propagation and convection of vortices.We consider a linearized formulation coupling an augmented Galbrun equation(for the perturbation of displacement)with a time harmonic convection equation(for the vortices).We first establish the well-posedness of this time harmonic convection equation in the appropriatemathematical framework.Then the complete problem,with Perfectly Matched Layers at the artificial boundaries,is proved to be coercive+compact,and a hybrid numerical method for the solution is proposed,coupling finite elements for the Galbrun equation and a Discontinuous Galerkin scheme for the convection equation.Finally a 2D numerical result shows the efficiency of the method.
