Pharmacological Investigation on the Qi-Invigorating Action of Schisandrin B: Effects on Mitochondrial ATP Generation in Multiple Tissues and Innate/Adaptive Immunity in Mice

Schisandrae Fructus, containing schisandrin B (Sch B) as its main active component, is recognized in traditional Chinese medicine (TCM) for its Qi-invigorating properties in the five visceral organs. Our laboratory has shown that the Qi-invigorating action of Chinese tonifying herbs is linked to increased mitochondrial ATP generation and an enhancement in mitochondrial glutathione redox status. To explore whether Sch B can exert Qi-invigorating actions across various tissues, we investigated the effects of Sch B treatment on mitochondrial ATP generation and glutathione redox status in multiple mouse tissues ex vivo. In line with TCM theory, which posits that Zheng Qi generation relies on the Qi function of the visceral organs, we also examined Sch B’s impact on natural killer cell activity and antigen-induced splenocyte proliferation, both serving as indirect measures of Zheng Qi. Our findings revealed that Sch B treatment consistently enhanced mitochondrial ATP generation and improved mitochondrial glutathione redox status in mouse tissues. This boost in mitochondrial function was associated with stimulated innate and adaptive immune responses, marked by increased natural killer cell activity and antigen-induced T/B cell proliferation, potentially through the increased generation of Zheng Qi.

Schisandrin B exerts anticancer effects on human gastric cancer cells through ROS-mediated MAPK,STAT3,and NF-κB pathways

Schisandrin B(Sch B)is a monomer with anti-cancer and anti-inflammatory effects,which are isolated from the plant Schisandra chinensis(Turcz)Baillon.We investigated the anti-gastric cancer(GC)effects of Sch B and its underlying molecular mechanisms.The Cell Counting Kit-8 assay was used to determine the effects of Sch B on the viability of GC and normal cell lines.Hoechst/propidium iodide staining and flow cytometry were used to assess the apoptosis induction of Sch B.Western blotting was used to evaluate the effects of Sch B on downstream apoptotic proteins.The DCFH-DA fluorescent probe was used to assess the regulatory effects of Sch B on reactive oxygen species(ROS)levels and related signaling pathways in GC cells.The results showed that Sch B could regulate the phosphorylation level of mitogen-activated protein kinase(MAPK)by upregulating ROS accumulation in gastric cancer cells,and then reduce the expression of nuclear factor kappa B(NF-κB)and phosphorylated transcription 3(p-STAT3).In addition,Sch B downregulated the cell cycle proteins cyclin-dependent kinase 2/4/6 and cyclin D1/E,and arrested cells in the G0/G1 phase.Moreover,it also inhibited cell migration,which was reversed with Nacetylcysteine pretreatment.In summary,Sch B has killing effects on GC cells by upregulating the production of intracellular ROS and regulating the MAPK/STAT3/NF-κB signaling pathway,leading to the migration arrest and apoptosis of GC cells.

Inhibitory effect of schisandrin B on gastric cancer cells in vitro

AIM： To investigate the inhibitory effect and possible mechanism of action of schisandrin B in SC-B on gastric cancer cells in vitro.METHODS： SC-B consisted of schisandrin B, aloeemodin, and Astragalus polysaccharides. Exponentially growing human gastric cancer SGO7901 cells were divided into six treatment groups： （1） control group （RPMI 1640 medium）; （2） negative control group （2% DMSO）; （3） positive control group （50 mg/L 5-Fluorouracil, 5-FU）; （4） low-dose group （LSC, final concentration of schisandrin B, 25 mg/L）, （5） moderate-dose group （MSC, final concentration of schisandrin B, 50 mg/L）; （6） highdose group （HSC, final concentration of schisandrin B, 100 mg/L）. Follow-up was done at 12-48 h. An MTT （Methylthiazolyldiphenyl-tetrazolium bromide） assay was used to examine the inhibitory effect of SOB on gastric cancer cells. The mitosis index was assessed using an inverted microscope. Flow cytometry was used to visualize the cell cycle. An RT-PCR （Reverse transcription-Polymerase chain reaction） -based assay was used to detect mRNA expression for cyclin D1 and glyceraldehyde-3-phosphate dehydrogenase （GAPDH）.RESULTS： The MTT assay showed that the number of living cells in the LSC, MSC and HSC groups was significantly smaller than that in the DMSO-treated group （P 〈 0.05） at 12-48 h. The inhibitory rate （IR） of the LSC group was 41.15% ± 3.86%, 59.24% ± 5.34% and 69.93% ± 7.81% at 12, 24 and 48 h, respectively. The IR of the MSC group was 42.82% ± 4.94%, 62.68% ± 7.58% and 71.79% ± 8.12% at 12, 24 and 48 h, respectively. The IR of the HSC group was 37.50% ± 3.21%, 40.34% ± 2.98% and 61.99% ± 4.88% at 12, 24 and 48 h, respectively. These results suggested that a moderate dosage had the most obvious inhibitory efficacy at 48 h. Compared to the DMSO group, the mitosis index of the LSC, MSC, HSC groups was greatly decreased （P 〈 0.05） at all time points. Any dose of SC-B suppressed mitosis within 12-48 h. Compared to the DMSO group, the percentage of cells in the G0/G1 phase of the MSC group was greatly increased, and that of the S ＋ G2M phase was greatly decreased, while the percentage of cell inhibition （PCI） in the MSC group was greatly increased （P 〈 0.05）. This suggested that SC-B could exclusively arrest cells in the G0/G1 phase. Cyclin D1 mRNA expression was lower in the MSC group than that in the DMSO group （P 〈 0.05）.CONCLUSION： SC-B can inhibit the proliferation and aberrant mitosis of human gastric cancer SCG-7901 cells /n v/tro, This inhibitory effect may be due to the down- regulation of cyclin D1 mRNA expression, which causes cell cycle arrest of gastric cancer cells.

Inhibitory effect of schisandrin B on free fatty acid-induced steatosis in L-02 cells

AIM:To investigate the effects of schisandrin B (Sch B) on free fatty acid (FFA)-induced steatosis in L-02 cells.METHODS:Cellular steatosis was induced by incubating L-02 cells with a FFA mixture (oleate and palmitate at the ratio of 2:1) for 24 h.Cytotoxicity and apoptosis were evaluated by 3-(4,5-dmethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and Annexin V/propidium iodide staining,respectively.Cellular total lipid was determined using a photocolorimetric method after Nile red staining,and triglyceride content was measured using an enzymatic kit.To study the effects of Sch B on steatosis,L-02 cells were treated with Sch B (1-100 μmol/L) in the absence or presence of 1 mmol/L FFA for 24 h,and cellular total lipid and triglyceride levels were measured.To explore the mechanisms of action of Sch B in the steatotic L-02 cells,mRNA levels of several regulators of hepatic lipid metabolism including adipose differentiation related protein (ADRP),sterol regulatory element binding protein 1 (SREBP-1),peroxisome proliferator-activated receptor (PPAR)-α and PPAR-γ were measured by quantitative real-time polymerase chain reaction (PCR),and protein levels of ADRP and SREBP-1 were measured by immunoblotting.RESULTS:Treatment with 1 mmol/L FFA for 24 h induced intracellular lipid accumulation in L-02 cells comparable to that in human steatotic livers without causing apparent apoptosis and cytotoxicity.Sch B mitigated cellular total lipid and triglyceride accumulations in the steatotic L-02 cells in a dose-dependent manner.Quantitative real-time PCR and Western blot analyses revealed that treatment of L-02 cells with 100 μmol/L Sch B reverted the FFA-stimulated up-regulation of ADRP and SREBP-1.CONCLUSION:Sch B inhibits FFA-induced steatosis in L-02 cells by,at least in part,reversing the up-regulation of ADRP and SREBP-1.

Schisandrin B protects PC12 cells by decreasing the expression of amyloid precursor protein and vacuolar protein sorting 35

PC12 cell injury was induced using 20 μM amyloid β-protein 25-35 to establish a model of Alzheimer＇s disease. The cells were then treated with 5, 10, and 25 μM Schisandrin B. Methylthiazolyldiphenyl-tetrazolium bromide assays and Hoechst 33342 staining results showed that with increasing Schisandrin B concentration, the survival rate of PC12 cells injured by amyloid β-protein 25-35 gradually increased and the rate of apoptosis gradually decreased. Reverse transcription-PCR, immunocytochemical staining and western blot results showed that with increasing Schisandrin B concentration, the mRNA and protein expression of vacuolar protein sorting 35 and amyloid precursor protein were gradually decreased. Vacuolar protein sorting 35 and amyloid precursor protein showed a consistent trend for change. These findings suggest that 5, 10, and 25 μM Schisandrin B antagonizes the cellular injury induced by amyloid β-protein 25-35 in a dose-dependent manner. This may be caused by decreasing the expression of vacuolar protein sorting 35 and amyloid precursor protein.

Study on HPLC method to determine contents of Schisandrin A and Schisandrin B in Schisandra chinensis extraction

The determination method of Schisandrin A and Schisandrin B in Schisandra chinensis was improved with the high performance liquid chromagraphy （HPLC）. The sample was extracted exceedingly in the critical limit of CO2. The retention time of Schisandrin A and Schisandrin B was reduced, with methano/water （75 ： 25） as mobile phase. The wavelength for detection was 254 nm. The R^2 of standard curve was 0.9998 and the relative standard deviation was 2.31% and 3.17% with the recovery of 96.45% and 97.37%, respectively. The result shows that the rate of veracity of this method is higher and it proves that the determination method of Sehisandrin A and Schisandrin B in Schisandra chinensis is a feasible method.

Investigation of in Vitro and in Vivo Metabolism of Schisandrin B from Schisandrae Fructus by Liquid Chromatography Coupled Electrospray Ionization Tandem Mass Spectrometry

Schisandrin B (Sch B) is one of the active dibenzocyclooctadiene lignans found in the Schisandrae Fructus. Experimental studies have shown that Sch B possesses various pharmacological properties, including anti-cancer, neuroprotective and nephroprotective activities. However, no detailed information on its biotransformation was reported in the literature. Here, we investigated the in vitro and in vivo metabolism of Sch B by using ultra-performance liquid chromatography coupled with tandem mass spectrometry. In vitro study detected and identified one oxygenated metabolite. Four metabolites were detected and identified from the in vivo study. The results indicated that the metabolism of Sch B mainly involved the demethylation of methoxy groups, the opening of five-member ring and the glucuronidation of metabolites in rats. The metabolites were identified for the first time by MS/MS analyses.

Novel discovery of schisandrin A regulating the interplay of autophagy and apoptosis in oligoasthenospermia by targeting SCF/c-kit and TRPV1 via biosensors

Oligoasthenospermia is the primary cause of infertility.However,there are still enormous challenges in the screening of critical candidates and targets of oligoasthenospermia owing to its complex mechanism.In this study,stem cell factor(SCF),c-kit,and transient receptor potential vanilloid 1(TRPV1)biosensors were successfully established and applied to studying apoptosis and autophagy mechanisms.Interestingly,the detection limit reached 2.787×10^(-15)g/L,and the quantitative limit reached 1.0×10^(-13)g/L.Furthermore,biosensors were used to investigate the interplay between autophagy and apoptosis.Schisandrin A is an excellent candidate to form a system with c-kit similar to SCF/c-kit with a detection constant(K_(D))of 5.701×10^(-11)mol/L,whereas it had no affinity for SCF.In addition,it also inhibited autophagy in oligoasthenospermia through antagonizing TRPV1 with a K_(D) of up to 4.181×10^(-10)mol/L.In addition,in vivo and in vitro experiments were highly consistent with the biosensor.In summary,high-potency schisandrin A and two potential targets were identified,through which schisandrin A could reverse the apoptosis caused by excessive autophagy during oligoasthenospermia.Our study provides promising insights into the discovery of effective compounds and potential targets via a well-established in vitro-in vivo strategy.

Schisandrin B Protects against Ischemic Brain Damage by Regulating PI3K/AKT Signaling in Rats

Objective To explore the effect and mechanism of schisandrin B(Sch B)in the treatment of cerebral ischemia in rats.Methods The cerebral ischemia models were induced by middle cerebral artery occlusion(MCAO)and reperfusion.Sprague-Dawley rats were divided into 6 groups using a random number table,including sham,MCAO,MCAO+Sch B(50 mg/kg),MCAO+Sch B(100 mg/kg),MCAO+Sch B(100 mg/kg)+LY294002,and MCAO+Sch B(100 mg/kg)+wortmannin groups.The effects of Sch B on pathological indicators,including neurological deficit scores,cerebral infarct volume,and brain edema,were subsequently studied.Tissue apoptosis was identified by terminal transferase-mediated dUTP nick end-labeling(TUNEL)staining.The protein expressions involved in apoptosis,inflammation response and oxidative stress were examined by immunofluorescent staining,biochemical analysis and Western blot analysis,respectively.The effect of Sch B on phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)signaling was also explored.Results Sch B treatment decreased neurological deficit scores,cerebral water content,and infarct volume in MCAO rats(P<0.05 or P<0.01).Neuronal nuclei and TUNEL staining indicated that Sch B also reduced apoptosis in brain tissues,as well as the Bax/Bcl-2 ratio and caspase-3 expression(P<0.01).Sch B regulated the production of myeloperoxidase,malondialdehyde,nitric oxide and superoxide dismutase,as well as the release of cytokine interleukin(IL)-1βand IL-18,in MCAO rats(P<0.05 or P<0.01).Sch B promoted the phosphorylation of PI3K and AKT.Blocking the PI3K/AKT signaling pathway with LY294002 or wortmannin reduced the protective effect of Sch B against cerebral ischemia(P<0.05 or P<0.01).Conclusions Sch B reduced apoptosis,inflammatory response,and oxidative stress of MCAO rats by modulating the PI3K/AKT pathway.Sch B had a potential for treating cerebral ischemia.

Chemoproteomics identifies Ykt6 as the direct target of schisandrin A for neuroprotection

Schisandrin A is a natural dibenzocyclooctene lignan with potent neuroprotective activity.However,the specific mechanisms or direct target proteins have not been clarified up to now.In this study,we designed and synthesized the probes of schisandrin A with photoreactive diazirine and clickable alkyne to identify its direct target in SH-SY5Y cells by employing activity-based protein profiling(ABPP)technique.Ykt6 was prominent among the 13 proteins obtained with high confidence and we confirmed Ykt6 as the direct target of schisandrin A by CETSA,IF,SPR and knockdown assay.Functionally,schisandrin A protected the cells against the injury induced by glutamate by regulating autophagy via Ykt6.This discovery may provide a novel therapeutic option for various neuronal cell damage-mediated diseases.

五味子乙素预处理对过氧化氢诱导的H9c2细胞焦亡及TXNIP/NLRP3/Caspase-1通路的影响

目的基于硫氧还蛋白互作蛋白(TXNIP)/核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)/半胱氨酸蛋白酶-1(Caspase-1)通路观察五味子乙素对过氧化氢(H 2O 2)诱导的大鼠H9c2心肌细胞氧化损伤及焦亡的影响,探讨五味子乙素的心肌保护作用机制。方法体外培养H9c2心肌细胞,实验设4组:正常组细胞常规孵育,五味子乙素组细胞加入40μmol/L五味子乙素孵育24 h,H 2O 2组细胞加入1000μmol/L的H 2O 2孵育30 min,H 2O 2+五味子乙素组细胞加入40μmol/L五味子乙素孵育24 h后再加入H 2O 2孵育30 min。CCK-8法检测细胞活力,DCFH-DA探针检测细胞中活性氧(ROS)含量,试剂盒检测细胞培养上清中乳酸脱氢酶(LDH)含量和细胞中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)含量,Western blot法检测细胞中TXNIP、硫氧还蛋白(Trx)、NLRP3、裂解的半胱氨酸蛋白酶-1(Cleaved Caspase-1)、消皮素D(GSDMD)-N蛋白表达情况,ELISA法检测细胞培养上清中白细胞介素-18(IL-18)、白细胞介素-1β(IL-1β)含量。结果与正常组比较,H 2O 2组细胞活力明显下降(P<0.05),细胞中ROS、MDA含量和TXNIP、NLRP3、Cleaved Caspase-1、GSDMD-N蛋白相对表达量及细胞培养上清中LDH、IL-1β、IL-18含量均明显升高(P均<0.05),细胞中SOD、GSH-Px含量和Trx蛋白相对表达量均明显降低(P均<0.05);与H 2O 2组比较,H 2O 2+五味子乙素组的细胞活力明显升高(P<0.05),细胞中ROS、MDA含量和TXNIP、NLRP3、Cleaved Caspase-1、GSDMD-N蛋白相对表达量及细胞培养上清中LDH、IL-1β、IL-18含量均明显降低(P均<0.05),细胞中SOD、GSH-Px含量和Trx蛋白相对表达量均明显升高(P均<0.05)。结论五味子乙素可能通过影响TXNIP/NLRP3/Caspase-1通路,从而减轻H 2O 2诱导的H9c2细胞氧化损伤,抑制心肌细胞焦亡。

五味子乙素通过TLR4/NF-κB信号通路对急性胰腺炎大鼠肺部损伤的影响

目的:探讨五味子乙素通过Toll样受体4(TLR4)/核转录因子-κB(NF-κB)信号通路对急性胰腺炎(AP)大鼠肺部损伤的影响。方法:取SD大鼠,通过胆胰管内逆行注射5%牛磺胆酸钠方法诱导建立AP肺损伤模型,经随机数表法分为模型组、五味子乙素组、TLR4过表达载体组、TLR4空载组、五味子乙素+TLR4过表达载体组,每组12只大鼠,再取12只SD大鼠仅翻动肠管不注射5%牛磺胆酸钠,作为假手术组。以药物分别干预大鼠后,检测各组大鼠肺功能及各组大鼠腹水量与肺组织湿重/干重(W/D);HE染色检测各组大鼠肺组织病理形态并评分;检测各组大鼠动脉血气;全自动生化分析仪检测大鼠血清淀粉酶,ELISA检测炎症细胞因子IL-6、IL-18水平;蛋白免疫印迹法检测肺组织TLR4/NF-κB通路蛋白表达;免疫组织化学染色检测肺组织TLR4蛋白表达。结果:与假手术组相比,模型组大鼠肺组织出现病理损伤改变,模型组大鼠MV、PEF、PaO_(2)、OI显著降低(P<0.05),Ri、腹水量与W/D、PaCO_(2)、Holfbauer评分、血清淀粉酶、IL-6与IL-18水平、肺组织TLR4阳性细胞比例、TLR4与MYD88蛋白表达、p-NF-κB p65/NF-κB p65水平显著升高(P<0.05)。与模型组、五味子乙素+TLR4过表达载体组分别相比,五味子乙素组大鼠肺组织病理损伤改变程度均减轻,MV、PEF、PaO_(2)、OI均升高(P<0.05),Ri、腹水量与W/D、PaCO_(2)、Holfbauer评分、血清淀粉酶、IL-6与IL-18水平、肺组织TLR4阳性细胞比例、TLR4与MYD88蛋白表达、p-NF-κB p65/NF-κB p65水平均降低(P<0.05);TLR4过表达载体组大鼠肺组织病理损伤改变程度均加重,MV、PEF、PaO_(2)、OI均降低(P<0.05),Ri、腹水量与W/D、PaCO_(2)、Holfbauer评分、血清淀粉酶、IL-6与IL-18水平、肺组织TLR4阳性细胞比例、TLR4与MYD88蛋白表达、p-NF-κB p65/NF-κB p65水平均升高(P<0.05)。与模型组相比,TLR4空载组大鼠各指标差异无统计学意义(P>0.05)。结论:五味子乙素可通过下调TLR4/NF-κB信号通路,抑制炎症,减轻AP大鼠肺部损伤,修复肺功能。

指纹图谱技术结合化学计量学评价五味子醇提物质量探索研究

目的:优化五味子乙醇提取工艺,建立其醇提物HPLC指纹图谱并结合化学计量学综合分析其质量。方法:基于单因素试验,采用L9(34)正交试验设计,系统研究了乙醇浓度、提取时间、提取次数及料液比等因素对五味子中五味子醇甲和五味子乙素的提取率产生的影响。按最优提取工艺制备9批五味子乙醇提取物,建立其高效液相色谱(HPLC)指纹图谱。以共有峰峰面积为变量,结合化学计量学方法包括系统聚类分析(HCA)、主成分分析(PCA)法对9批五味子醇提物质量进行分析。结果:确定最佳提取工艺为:采用80%乙醇作为溶剂,料液比为1:10,进行2次回流提取,每次持续1 h。在9批五味子提取物指纹图谱中共有32个共有峰,并成功鉴定出X18(五味子醇甲)、X29(五味子甲素)和X32(五味子乙素),相似度高于0.94。HCA和PCA结果均显示9批样品有聚为四类的趋势,共有峰X1、X2、X3、X13、X17、X19、X21和X30是引起样品间差异的主要化学成分。结论:所优选的提取工艺稳定可行,不同批次五味子提取物的质量一致性较高,但不同样品间仍存在一定差异。

How Schisandrae Fructus Benefits the Body: Mechanisms in Traditional Chinese Medicine and Modern Medicine

Schisandrae chinensis Fructus (SF) is a commonly used herb in Traditional Chinese Medicine (TCM). According to TCM theory, SF can invigorate Qi in the liver and other visceral organs through the meridian system. Furthermore, the liver’s pivotal role in regulating the functions of various visceral organs helps explain how SF can promote holistic health benefits. The main active ingredient of SF, schisandrin B (Sch B), has been found to improve mitochondrial ATP production and enhance glutathione redox status in multiple organs. This could account for the overall protective effects of Sch B on organs. Due to its stronger impact on liver function, the positive influence of Sch B on different organs may be facilitated by signal molecules originating from the liver.

基于TLR2/MyD88信号通路探究五味子乙素对全身炎症反应综合征的影响

目的观察五味子乙素(Schisandrin B,SchB)通过TLR2/MyD88信号通路对酵母聚糖诱导的小鼠全身炎症反应综合征(Systemic inflammatory response syndrome,SIRS)的影响。方法采用腹腔注射酵母聚糖方法造模,记录小鼠体温及白细胞变化,应用酶联免疫吸附法检测血清中TNF-α、IL-1β及IL-6水平,应用试剂盒检测ALT、AST、Cr、BUN含量;HE染色观察各组织病理学变化;应用Western Blot法检测组织中TLR2、MyD88、NF-κB、HMGB1蛋白表达水平。结果SchB使小鼠体温升高显著(P<0.01),白细胞数量增多明显(P<0.01);SchB能显著降低TNF-α、IL-1β、IL-6、ALT、AST、Cr、BUN水平(P<0.001);Western Blot结果显示,SchB能显著降低组织中TLR2、MyD88、NF-κB、HMGB1的表达水平(P<0.01)。结论SchB能够减轻由酵母聚糖导致的SIRS,其作用机制主要是通过调控TLR2/MyD88信号通路缓解炎症。

冰片修饰五味子乙素胶束处方优选及体外对bEnd.3细胞靶向性评价研究

目的优化冰片修饰五味子乙素胶束(Borneol-Schisandrin B-Micelles,Bor-Sch B-Ms)处方,并对其体外脑靶向作用进行初步探索。方法采用薄膜分散法制备Bor-Sch B-Ms;对Soluplus/TPGS1000质量比(mg/mg)、Soluplus/五味子乙素质量比(mg/mg)和水化温度(℃)进行优化,确定Bor-Sch B-Ms的最优处方制备工艺;并对最优处方工艺制得的胶束进行表征;采用高效液相色谱法(HPLC)对胶束中五味子乙素进行含量测定,以测得Bor-Sch B-Ms的包封率;利用不同药物对小鼠脑内血管内皮细胞(bEnd.3)的荧光摄取实验进行Bor-Sch B-Ms体外靶向性评价。结果最优处方为Soluplus 120 mg,Soluplus/TPGS1000=2∶1,Soluplus/五味子乙素=12∶1,DSPE-PEG20002 mg,水化温度40℃,处方量为5 mL。所得最优胶束的粒径为(93.72±0.65)nm,电位为(-2.73±0.35)mV,Bor-Sch B-Ms的包封率为(93.54±0.86)%。体外细胞摄取实验显示,与五味子乙素胶束组(Sch B-Ms)相比,Bor-Sch B-Ms组细胞的荧光强度明显增加(P<0.05)。结论通过Box-Behnken设计-响应面法确定Bor-Sch B-Ms最优处方,制备得到的Bor-Sch B-Ms具有潜在的脑靶向性。

天然产物五味子乙素对口腔癌细胞增殖、凋亡及侵袭的影响

目的 研究天然产物五味子乙素(Schisandrin B,Sch B)对口腔癌细胞增殖、凋亡和侵袭的影响。方法 用不同浓度的五味子乙素分别处理口腔癌细胞株SCC15和CAL27细胞24 h和48 h,采用CCK-8法和AnnexinV-FITC/PI双标记流式细胞术,检测五味子乙素对口腔癌细胞增殖及凋亡的影响;采用Transwell实验分别检测五味子乙素对细胞侵袭的影响,采用qRT-PCR和Western Blot检测五味子乙素对Prx1 mRNA与蛋白表达的影响。结果 CCK-8和流式细胞术检测发现五味子乙素明显抑制SCC15和CAL27细胞增殖并促进细胞凋亡,呈时间和剂量依赖性(P<0.05)。Transwell实验结果显示,五味子乙素对侵袭的抑制作用呈时间和剂量依赖性。五味子乙素对Prx1 mRNA的表达无显著影响,但对其蛋白表达有显著的抑制作用(P<0.05)。结论 五味子乙素可能通过调控Prx1蛋白的表达抑制口腔癌细胞的增殖、侵袭,促进细胞凋亡。

辽宁道地药材北五味子的蜜制工艺优化及质量标准评价

目的正交试验优选辽宁道地药材北五味子的蜜制工艺,并对蜜五味子炮制品进行质量标准评价。方法研究收集10个不同厂家生产的共10批生北五味子,采用蜜蒸法炮制蜜五味子,以五味子醇甲的含量作为考察指标,采用正交试验优选蜜五味子的最佳炮制工艺;研究对蜜五味子进行性状、水分、总灰分检查;采用薄层色谱法对蜜五味子进行定性鉴别;采用高效液相色谱法(high performance liquid chromatography,HPLC)对蜜五味子中的五味子醇甲进行含量测定。结果正交试验得出蜜五味子的最佳炮制工艺条件:闷润时间1 h,炼蜜量为每100 kg五味子,用10 kg炼蜜,蜜水比例为1∶2,蒸煮时间为40 min。所得10批蜜五味子均性状较佳;该品含水量为12.95%,总灰分为3.36%,均未超过《中华人民共和国药典》标准的16.0%和7.0%;薄层色谱显示10批蜜五味子样品中五味子醇甲均斑点清晰,分离效果好;HPLC色谱检测得出10批蜜五味子样品中五味子醇甲的含量为0.48%~0.70%,平均值为0.59%,高于《中华人民共和国药典》中的含量限度0.40%。结论研究所得北五味子的蜜制工艺稳定可行,建立的质控标准准确度高、专属性强、重复性好,可作为蜜五味子炮制品的质量评价标准。

Network pharmacology-based prediction and verification of the molecular targets and pathways for schisandrin against cerebrovascular disease

AIM： To illuminate the molecular targets for schisandrin against cerebrovascular disease based on the combined methods of network pharmacology prediction and experimental verification. METHOD： A protein database was established through constructing the drug-protein network from literature mining data. The protein-protein network was built through an in-depth exploration of the relationships between the proteins. The computational platform was implemented to predict and extract the sensitive sub-network with significant P-values from the protein-protein network. Then the key targets and pathways were identified from the sensitive sub-network. The most related targets and pathways were also confirmed in hydrogen peroxide （H202）-induced PC 12 cells by Western blotting. RESULTS： Twelve differentially expressed proteins （gene names： NFKB1, RELA, TNFSF10, MAPK1, CHUK, CASP8, PIGS2, MAPK 14, CREBI, IFNG, APR and BCL2） were confirmed as the central nodes of the interaction network （45 nodes, 93 edges）. The NF-KB signaling pathway was suggested as the most related pathway of schisandrin for cerebrovascular disease. Furthermore, schisandrin was found to suppress the expression and phosphorylation of 1KKct, as well as p50 and p65 induced by H2O2 in PC12 cells by Western blotting. CONCLUSION： The computational platform that integrates literature mining data, protein-protein interactions, sensitive sub-network, and pathway results in identification of the NF-arB signaling pathway as the key targets and pathways for schisandrin.

五味子醇甲对鸡大肠杆菌病的预防效果观察

旨在明确五味子醇甲对鸡大肠杆菌病的预防作用,将180只1日龄蛋鸡随机分为6组:空白对照组、E.coli感染组、50 mg·kg^(-1)五味子醇甲组、100 mg·kg^(-1)五味子醇甲组、200 mg·kg^(-1)五味子醇甲组和抗生素组(氟苯尼考)。饲养至14 d时,按分组剂量开始给药,给药5 d后,除空白对照组外其余各组均腹腔接种大肠杆菌菌液,检测雏鸡体重、免疫器官指数、脾病理变化和超微结构、血细胞数、血清炎症因子、抗体水平和免疫球蛋白的变化。结果表明,五味子醇甲能够提高患病雏鸡体重,显著降低脾指数,减轻由于大肠杆菌感染导致的脾组织结构以及细胞中细胞核和线粒体的损伤,显著降低患病鸡的白细胞数量以及血清中TNF-α、IL-1β、IL-6和IL-8水平,并显著升高IgM、IgG和NDV的抗体水平。综上所述,五味子醇甲能够通过调节免疫对鸡大肠杆菌病起抗病作用。