目的构建并验证一种头颈鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)自噬相关基因预后风险评分模型。方法从癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库下载全部HNSCC转录组表达数据(RNA sequencing,RNA-seq)及...目的构建并验证一种头颈鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)自噬相关基因预后风险评分模型。方法从癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库下载全部HNSCC转录组表达数据(RNA sequencing,RNA-seq)及临床信息,筛选出差异表达基因,与GeneCards数据库检索的自噬相关基因(autophagy related genes,ARGs)取交集,得到差异表达的ARGs,整合临床信息后经预后分析获得预后相关的ARGs,再对其富集分析。应用套索(the least absolute shrinkage and selection operator,LASSO)回归和Cox回归模型构建一种用于预测HNSCC预后及生存情况的风险评分模型;绘制受试者工作特征(receiver operating characteristic,ROC)曲线,计算曲线下面积(area under the curve,AUC)及最佳截断(cut-off)值,并以最佳cut-off值将患者分为高、低风险评分组;绘制Kaplan-Meier生存曲线评价该模型的预测性能;将临床信息与风险评分对应整合,再次利用Cox回归分析评价风险评分的独立预后价值。结果通过对差异表达的ARGs进行预后分析初筛出20个与预后相关的ARGs,再应用LASSO回归和Cox回归分析获得9个与预后显著相关的ARGs,以此构建HNSCC的预后风险评分模型。ROC曲线及Kaplan-Meier生存曲线分析显示,低风险评分组生存时间优于高风险评分组,两组生存时间差异有统计学意义(P<0.001),且该模型在训练集(AUC最大值为0.69)与外部验证集(AUC最大值为0.822)中均展现出良好的预测性能。纳入临床信息后,对风险评分进行Cox回归分析,提示其与HNSCC患者预后显著相关(P<0.001),表明风险评分对HNSCC具有独立预后价值。结论由9个ARGs组成的HNSCC风险评分模型,可有效预测HNSCC患者的预后情况。展开更多
Sand and dust storms （SDS） are common phenomena in arid and semi-arid areas. In recent years, SDS frequencies and intensities have increased significantly in Iran. A research on SDS sources is important for understa...Sand and dust storms （SDS） are common phenomena in arid and semi-arid areas. In recent years, SDS frequencies and intensities have increased significantly in Iran. A research on SDS sources is important for understanding the mechanisms of dust generation and assessing its socio-economic and environmental impacts. In this paper, we developed a new approach to identify SDS source areas in Iran using a combination of nine related datasets, namely drought events, temperature, precipitation, location of sandy soils, SDS frequency, hu- man-induced soil degradation （HISD）, human influence index （HII）, rain use efficiency （RUE） and net primary pro- ductivity （NPP） loss. To identify SDS source areas, we firstly normalized these datasets under uniform criteria in- cluding layer reprojection using Lambert conformal conic projection, data conversion from shapefile to raster, Min-Max Normalization with data range from 0 to 1, and data interpolation by Kriging and images resampling （resolution of 1 km）. After that, a score map for the possibility of SDS sources was generated through overlaying multiple datasets under average weight allocation criterion, in which each item obtained weight equally. In the score map, the higher the score, the more possible a specific area could be regarded as SDS source area. Exceptions mostly came from large cities, like Tehran and Isfahan. As a result, final SDS source areas were mapped out, and Al-Howizeh/Al-Azim marshes and Sistan Basin were identified as main SDS source areas in Iran. The SDS source area in Al-Howizeh/Al-Azim marshes still keeps expanding. In addition, Al-Howizeh/Al-Azim marshes are now suf- fering rapid land degradation due to natural and human-induced factors and might totally vanish in the near future. Sistan Basin also demonstrates the impacts of soil degradation and wind erosion. With appropriate intensity, dura- tion, wind speed and altitude of the dust storms, sand particles uplifting from this area might have developed into extreme dust storms, especially during the summer.展开更多
目的:对比传统口头健康教育与思维导图结合多媒体健康教育对髋关节置换术后并发症发生率、Harris评分和Barthel指数评分以及医护服务满意度的影响,探讨思维导图结合多媒体健康教育模式对人工全髋关节置换术(total hip arthroplasty,THA...目的:对比传统口头健康教育与思维导图结合多媒体健康教育对髋关节置换术后并发症发生率、Harris评分和Barthel指数评分以及医护服务满意度的影响,探讨思维导图结合多媒体健康教育模式对人工全髋关节置换术(total hip arthroplasty,THA)患者术后康复效果的影响。方法:将2019年1月至2020年12月马鞍山市人民医院骨科收治的首次行THA的患者随机分成观察组与对照组,对照组患者给予常规口头健康宣教,观察组采用自行设计的思维导图结合多媒体技术进行健康宣教。宣教内容包括知识宣教、康复锻炼、并发症的预防。统计2组患者术后1周、1个月、3个月、6个月Harris评分和Barthel指数评分、术后并发症发生率以及患者满意度,并进行客观评价。结果:与对照组比较,观察组患者手术后Harris评分和Barthel指数评分均增加,差异有统计学意义(P<0.05),且采用思维导图结合多媒体技术进行健康教育干预时间越长,2组间差异越显著;观察组患者术后并发症总发生率显著低于对照组患者(P=0.006);观察组患者术后医护服务满意度显著高于对照组患者(P=0.003)。结论:采用思维导图结合多媒体技术对人工THA术后患者进行健康宣教指导,可有效提升患者术后康复效果,提高患者术后满意度,改善护患关系,值得临床推广应用。展开更多
文摘目的构建并验证一种头颈鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)自噬相关基因预后风险评分模型。方法从癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库下载全部HNSCC转录组表达数据(RNA sequencing,RNA-seq)及临床信息,筛选出差异表达基因,与GeneCards数据库检索的自噬相关基因(autophagy related genes,ARGs)取交集,得到差异表达的ARGs,整合临床信息后经预后分析获得预后相关的ARGs,再对其富集分析。应用套索(the least absolute shrinkage and selection operator,LASSO)回归和Cox回归模型构建一种用于预测HNSCC预后及生存情况的风险评分模型;绘制受试者工作特征(receiver operating characteristic,ROC)曲线,计算曲线下面积(area under the curve,AUC)及最佳截断(cut-off)值,并以最佳cut-off值将患者分为高、低风险评分组;绘制Kaplan-Meier生存曲线评价该模型的预测性能;将临床信息与风险评分对应整合,再次利用Cox回归分析评价风险评分的独立预后价值。结果通过对差异表达的ARGs进行预后分析初筛出20个与预后相关的ARGs,再应用LASSO回归和Cox回归分析获得9个与预后显著相关的ARGs,以此构建HNSCC的预后风险评分模型。ROC曲线及Kaplan-Meier生存曲线分析显示,低风险评分组生存时间优于高风险评分组,两组生存时间差异有统计学意义(P<0.001),且该模型在训练集(AUC最大值为0.69)与外部验证集(AUC最大值为0.822)中均展现出良好的预测性能。纳入临床信息后,对风险评分进行Cox回归分析,提示其与HNSCC患者预后显著相关(P<0.001),表明风险评分对HNSCC具有独立预后价值。结论由9个ARGs组成的HNSCC风险评分模型,可有效预测HNSCC患者的预后情况。
基金funded by the Small Scale Funding Agreement (UNEP/ROWA)
文摘Sand and dust storms （SDS） are common phenomena in arid and semi-arid areas. In recent years, SDS frequencies and intensities have increased significantly in Iran. A research on SDS sources is important for understanding the mechanisms of dust generation and assessing its socio-economic and environmental impacts. In this paper, we developed a new approach to identify SDS source areas in Iran using a combination of nine related datasets, namely drought events, temperature, precipitation, location of sandy soils, SDS frequency, hu- man-induced soil degradation （HISD）, human influence index （HII）, rain use efficiency （RUE） and net primary pro- ductivity （NPP） loss. To identify SDS source areas, we firstly normalized these datasets under uniform criteria in- cluding layer reprojection using Lambert conformal conic projection, data conversion from shapefile to raster, Min-Max Normalization with data range from 0 to 1, and data interpolation by Kriging and images resampling （resolution of 1 km）. After that, a score map for the possibility of SDS sources was generated through overlaying multiple datasets under average weight allocation criterion, in which each item obtained weight equally. In the score map, the higher the score, the more possible a specific area could be regarded as SDS source area. Exceptions mostly came from large cities, like Tehran and Isfahan. As a result, final SDS source areas were mapped out, and Al-Howizeh/Al-Azim marshes and Sistan Basin were identified as main SDS source areas in Iran. The SDS source area in Al-Howizeh/Al-Azim marshes still keeps expanding. In addition, Al-Howizeh/Al-Azim marshes are now suf- fering rapid land degradation due to natural and human-induced factors and might totally vanish in the near future. Sistan Basin also demonstrates the impacts of soil degradation and wind erosion. With appropriate intensity, dura- tion, wind speed and altitude of the dust storms, sand particles uplifting from this area might have developed into extreme dust storms, especially during the summer.
文摘目的:对比传统口头健康教育与思维导图结合多媒体健康教育对髋关节置换术后并发症发生率、Harris评分和Barthel指数评分以及医护服务满意度的影响,探讨思维导图结合多媒体健康教育模式对人工全髋关节置换术(total hip arthroplasty,THA)患者术后康复效果的影响。方法:将2019年1月至2020年12月马鞍山市人民医院骨科收治的首次行THA的患者随机分成观察组与对照组,对照组患者给予常规口头健康宣教,观察组采用自行设计的思维导图结合多媒体技术进行健康宣教。宣教内容包括知识宣教、康复锻炼、并发症的预防。统计2组患者术后1周、1个月、3个月、6个月Harris评分和Barthel指数评分、术后并发症发生率以及患者满意度,并进行客观评价。结果:与对照组比较,观察组患者手术后Harris评分和Barthel指数评分均增加,差异有统计学意义(P<0.05),且采用思维导图结合多媒体技术进行健康教育干预时间越长,2组间差异越显著;观察组患者术后并发症总发生率显著低于对照组患者(P=0.006);观察组患者术后医护服务满意度显著高于对照组患者(P=0.003)。结论:采用思维导图结合多媒体技术对人工THA术后患者进行健康宣教指导,可有效提升患者术后康复效果,提高患者术后满意度,改善护患关系,值得临床推广应用。
