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Weakly Supervised Network with Scribble-Supervised and Edge-Mask for Road Extraction from High-Resolution Remote Sensing Images
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作者 Supeng Yu Fen Huang Chengcheng Fan 《Computers, Materials & Continua》 SCIE EI 2024年第4期549-562,共14页
Significant advancements have been achieved in road surface extraction based on high-resolution remote sensingimage processing. Most current methods rely on fully supervised learning, which necessitates enormous human... Significant advancements have been achieved in road surface extraction based on high-resolution remote sensingimage processing. Most current methods rely on fully supervised learning, which necessitates enormous humaneffort to label the image. Within this field, other research endeavors utilize weakly supervised methods. Theseapproaches aim to reduce the expenses associated with annotation by leveraging sparsely annotated data, such asscribbles. This paper presents a novel technique called a weakly supervised network using scribble-supervised andedge-mask (WSSE-net). This network is a three-branch network architecture, whereby each branch is equippedwith a distinct decoder module dedicated to road extraction tasks. One of the branches is dedicated to generatingedge masks using edge detection algorithms and optimizing road edge details. The other two branches supervise themodel’s training by employing scribble labels and spreading scribble information throughout the image. To addressthe historical flaw that created pseudo-labels that are not updated with network training, we use mixup to blendprediction results dynamically and continually update new pseudo-labels to steer network training. Our solutiondemonstrates efficient operation by simultaneously considering both edge-mask aid and dynamic pseudo-labelsupport. The studies are conducted on three separate road datasets, which consist primarily of high-resolutionremote-sensing satellite photos and drone images. The experimental findings suggest that our methodologyperforms better than advanced scribble-supervised approaches and specific traditional fully supervised methods. 展开更多
关键词 Semantic segmentation road extraction weakly supervised learning scribble supervision remote sensing image
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BIM联合Scribble预测晚期非小细胞肺癌化疗效果 被引量:4
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作者 张龙富 姚家美 +2 位作者 蒋冬先 侯英勇 张新 《中国癌症杂志》 CAS CSCD 北大核心 2020年第11期865-871,共7页
背景与目的:BIM基因和Scribble均是参与细胞凋亡的重要介质。BIM基因的BH3域缺失,可引起凋亡受阻。Scribble低表达对肿瘤细胞增殖、肿瘤转移和耐药有促进作用。通过检测BIM基因多态性和Scribble表达,探讨其与晚期非小细胞肺癌(non-small... 背景与目的:BIM基因和Scribble均是参与细胞凋亡的重要介质。BIM基因的BH3域缺失,可引起凋亡受阻。Scribble低表达对肿瘤细胞增殖、肿瘤转移和耐药有促进作用。通过检测BIM基因多态性和Scribble表达,探讨其与晚期非小细胞肺癌(non-small cell lung cancer,NSCLC)化疗效果的关系。方法:收集2014年1月—2015年12月于复旦大学附属中山医院就诊的96例晚期NSCLC患者,所有患者均一线接受含铂方案化疗。采用聚合酶链反应(polymerase chain reaction,PCR)检测NSCLC患者组织标本中的BIM基因多态性,采用免疫组织化学法检测标本中的Scribble表达水平,然后评估BIM基因多态性和Scribble表达与化疗疗效的关系。结果:BIM基因野生型组化疗的中位无进展生存期(progression-free survival,PFS)优于BIM基因缺失组(5.0个月vs 2.7个月,P=0.01)。Scribble高表达组化疗的中位PFS优于Scribble低表达组(7.0个月vs 4.0个月,P<0.001)。BIM基因野生型且Scribble高表达组(BIM-WT-Scrib-H)化疗的中位PFS优于BIM基因野生型或Scribble高表达组(BIM-WT/Scrib-H)和BIM基因缺失型且Scribble低表达组(BIM-del-Scrib-L)(10.0个月vs 4.5个月vs 2.0个月,P<0.001)。多因素分析结果显示,BIM基因缺失型为化疗具有更短PFS的独立预测因素(HR=3.221,P<0.001);Scribble低表达为化疗具有更短PFS的独立预测因素(HR=3.312,P<0.001)。结论:BIM基因缺失型和Scribble低表达是晚期NSCLC化疗效果不佳的独立预测因素,两者联合应用有更好的预测价值。 展开更多
关键词 BIM基因 scribble 非小细胞肺癌 化疗
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Scribble在原肠期鸡胚表达的研究
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作者 徐涛 林艳青 +5 位作者 王晓钰 吴婷 韩哲 张希敏 杨雪松 王丽京 《生物技术通报》 CAS CSCD 北大核心 2011年第7期176-179,共4页
旨在研究极性脚手架蛋白Scibble(Scrib)在原肠期的表达及意义,明确Scrib在早期鸡胚发育中的作用。以含有全长人Scrib的质粒pEGFP-N2-Scrib作为模板克隆出N端一段770 bp左右的片段,从而构建一个新的质粒pSPT18-Scrib;以pSPT18-Scrib为模... 旨在研究极性脚手架蛋白Scibble(Scrib)在原肠期的表达及意义,明确Scrib在早期鸡胚发育中的作用。以含有全长人Scrib的质粒pEGFP-N2-Scrib作为模板克隆出N端一段770 bp左右的片段,从而构建一个新的质粒pSPT18-Scrib;以pSPT18-Scrib为模板进行体外转录制备cRNA探针;并采用原位杂交的方法用此探针检测鸡原肠胚各个时期Scrib的表达情况。结果显示,Scrib在鸡胚四期开始逐渐在原条顶端及两侧的外胚层表达,并随着发育过程向外胚层两侧蔓延扩散,并且在发展到十期时呈现包括神经管和体节在内的广泛的弥散性表达。Scrib的表达规律提示在胚胎发育早期Scrib对外胚层细胞迁移和分化以及而后的器官发生起到重要的作用,为进一步研究Scrib在鸡胚早期发育中的作用提供参考。 展开更多
关键词 scribble CRNA探针 原肠期 原位杂交
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Scribble-Supervised Video Object Segmentation 被引量:3
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作者 Peiliang Huang Junwei Han +2 位作者 Nian Liu Jun Ren Dingwen Zhang 《IEEE/CAA Journal of Automatica Sinica》 SCIE EI CSCD 2022年第2期339-353,共15页
Recently,video object segmentation has received great attention in the computer vision community.Most of the existing methods heavily rely on the pixel-wise human annotations,which are expensive and time-consuming to ... Recently,video object segmentation has received great attention in the computer vision community.Most of the existing methods heavily rely on the pixel-wise human annotations,which are expensive and time-consuming to obtain.To tackle this problem,we make an early attempt to achieve video object segmentation with scribble-level supervision,which can alleviate large amounts of human labor for collecting the manual annotation.However,using conventional network architectures and learning objective functions under this scenario cannot work well as the supervision information is highly sparse and incomplete.To address this issue,this paper introduces two novel elements to learn the video object segmentation model.The first one is the scribble attention module,which captures more accurate context information and learns an effective attention map to enhance the contrast between foreground and background.The other one is the scribble-supervised loss,which can optimize the unlabeled pixels and dynamically correct inaccurate segmented areas during the training stage.To evaluate the proposed method,we implement experiments on two video object segmentation benchmark datasets,You Tube-video object segmentation(VOS),and densely annotated video segmentation(DAVIS)-2017.We first generate the scribble annotations from the original per-pixel annotations.Then,we train our model and compare its test performance with the baseline models and other existing works.Extensive experiments demonstrate that the proposed method can work effectively and approach to the methods requiring the dense per-pixel annotations. 展开更多
关键词 Convolutional neural networks(CNNs) scribble self-attention video object segmentation weakly supervised
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Scribble和NADPH氧化酶复合体的相互作用调控M1巨噬细胞的极化和功能
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作者 黎凯颖 《中国病理生理杂志》 CAS CSCD 北大核心 2017年第2期288-288,共1页
极性蛋白Scribble(SCRIB)在果蝇和哺乳动物中调节(细胞)顶端基底的极性、定向迁移和肿瘤抑制。Zheng等发现SCRIB是髓细胞功能(包括细菌感染和炎症)一个重要的调控因子。SCRIB通过依赖PSD95/Dlg/ZO-1(PDZ)结构域的方式与NADPH氧... 极性蛋白Scribble(SCRIB)在果蝇和哺乳动物中调节(细胞)顶端基底的极性、定向迁移和肿瘤抑制。Zheng等发现SCRIB是髓细胞功能(包括细菌感染和炎症)一个重要的调控因子。SCRIB通过依赖PSD95/Dlg/ZO-1(PDZ)结构域的方式与NADPH氧化酶(NADPH oxidase,NOX)复合体直接相互作用,而且在体外培养和活体中, 展开更多
关键词 NADPH M1 scribble 复合体 直接相互作用 巨噬细胞 活性氧簇 髓细胞 体外培养 细菌感染
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Meibomian glands segmentation in infrared images with limited annotation
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作者 Jia-Wen Lin Ling-Jie Lin +5 位作者 Feng Lu Tai-Chen Lai Jing Zou Lin-Ling Guo Zhi-Ming Lin Li Li 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2024年第3期401-407,共7页
●AIM:To investigate a pioneering framework for the segmentation of meibomian glands(MGs),using limited annotations to reduce the workload on ophthalmologists and enhance the efficiency of clinical diagnosis.●METHODS... ●AIM:To investigate a pioneering framework for the segmentation of meibomian glands(MGs),using limited annotations to reduce the workload on ophthalmologists and enhance the efficiency of clinical diagnosis.●METHODS:Totally 203 infrared meibomian images from 138 patients with dry eye disease,accompanied by corresponding annotations,were gathered for the study.A rectified scribble-supervised gland segmentation(RSSGS)model,incorporating temporal ensemble prediction,uncertainty estimation,and a transformation equivariance constraint,was introduced to address constraints imposed by limited supervision information inherent in scribble annotations.The viability and efficacy of the proposed model were assessed based on accuracy,intersection over union(IoU),and dice coefficient.●RESULTS:Using manual labels as the gold standard,RSSGS demonstrated outcomes with an accuracy of 93.54%,a dice coefficient of 78.02%,and an IoU of 64.18%.Notably,these performance metrics exceed the current weakly supervised state-of-the-art methods by 0.76%,2.06%,and 2.69%,respectively.Furthermore,despite achieving a substantial 80%reduction in annotation costs,it only lags behind fully annotated methods by 0.72%,1.51%,and 2.04%.●CONCLUSION:An innovative automatic segmentation model is developed for MGs in infrared eyelid images,using scribble annotation for training.This model maintains an exceptionally high level of segmentation accuracy while substantially reducing training costs.It holds substantial utility for calculating clinical parameters,thereby greatly enhancing the diagnostic efficiency of ophthalmologists in evaluating meibomian gland dysfunction. 展开更多
关键词 infrared meibomian glands images meibomian gland dysfunction meibomian glands segmentation weak supervision scribbled annotation
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Scribble调控β-catenin的入核
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作者 李苏瑞 牛晓峰 陈铭 《中国细胞生物学学报》 CAS CSCD 2010年第3期433-438,共6页
Scribble(Scrib)是富含亮氨酸重复序列及PDZ结构域蛋白(LAP)家族的一员,以脚手架蛋白的方式与多种分子相结合并整合多种信号通路协同发挥作用。β-catenin是一种多功能蛋白质,在介导细胞粘附及信号转导中起中心分子作用。为研究Scrib与... Scribble(Scrib)是富含亮氨酸重复序列及PDZ结构域蛋白(LAP)家族的一员,以脚手架蛋白的方式与多种分子相结合并整合多种信号通路协同发挥作用。β-catenin是一种多功能蛋白质,在介导细胞粘附及信号转导中起中心分子作用。为研究Scrib与β-catenin间是否存在联系及何种联系,我们做了一系列实验。研究发现,如果HEK293/HEK293T细胞中过表达Scrib,β-catenin的转录活性报告基因LEF-1-1uciferase的活性则显著减弱;而如果HEK293/HEK293T细胞中稳定下调Scrib,LEF-1-luciferase的活性则显著增强。表明Scrib可抑制β-catenin的转录活性。我们同时利用免疫共沉淀(Co-IP)实验发现Scrib与β-catenin存在特异结合作用。另外,细胞组分分离实验表明HEK293/HEK293T细胞中稳定下调Scrib,细胞中β-catenin蛋白总量不变,而胞浆及胞核内β-catenin量增多即表明Scrib可抑制β-catenin的入核。本研究在分子机制上表明Scrib可通过与β-catenin的结合,抑制β-catenin入核,从而影响β-catenin的下游调控基因。 展开更多
关键词 scribble Β-CATENIN LEF-1-luciferase 入核
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Deciphering tumor-suppressor signaling in flies:Genetic link between Scribble/Dlg/Lgl and the Hippo pathways
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作者 Masato Enomoto Tatsushi Igaki 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2011年第10期461-470,共10页
Loss of apico-basal polarity is one of the crucial factors that drives epithelial tumor progression, scribbleldiscs largellethal giant larvae (scrib/dlg/lgl), a group of apico-basal polarity genes, were initially id... Loss of apico-basal polarity is one of the crucial factors that drives epithelial tumor progression, scribbleldiscs largellethal giant larvae (scrib/dlg/lgl), a group of apico-basal polarity genes, were initially identified as members of "neoplastic" tumor-suppressors in flies. The components of the Hippo signaling pathway, which is crucial for organ size control and cancer development, were also identified through Drosophila genetic screens as members of "hyperplastic" tumor-suppressors. Accumulating evidence in recent studies implies that these two tumor-suppressor signaling pathways are not mutually exclusive but rather cooperatively act to give rise to highly malignant tumors. The interaction of these tumor-suppressor pathways could include deregulations of actin cytoskeleton, cell-cell contact, and apical-domain size of 展开更多
关键词 Cell polarity Hippo pathway DROSOPHILA scribble DLG lgl TUMOR-SUPPRESSOR Tumor progression
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