Seabuckthorn juice alleviates allergic symptoms in shrimp-induced food allergy mice

Tropomyosin(TM)in shrimp is one of the predominant causes of food allergy around the world.In the present study,the effect of seabuckthorn juice against TM-induced shrimp allergy was investigated in BALB/c mice.Allergic symptoms,spleen index,intestinal section and diarrhea were measured in shrimp allergy mice.As the results,seabuckthorn juice suppressed the lesions in jejunum tissue,diarrhea and allergic symptoms in shrimp allergy mice.Seabuckthorn juice also reduced serum concentrations of tumor necrosis factor-a(TNF-α)as well as immunoglobulin E(IgE)and stimulated the secretion of interleukin-10(IL-10)in mice with shrimp allergy.Taken together,our findings suggest that increased IL-10 by seabuckthorn juice inhibits Th2 cytokine production to suppress shrimp allergic symptoms.Furthermore,seabuckthorn juice also regulates shrimp allergy by reducing jejunum lesions,inhibiting levels of TNF-αand IgE.

Deep eutectic solvents and alkaline extraction of protein from seabuckthorn seed meal: a comparison study

Seabuckthorn seed meal(SSM) is a waste of oil extraction industry that rich in protein. In order to seek suitable protein extraction method, three different deep eutectic solvents(DESs)(including choline chlorideglycerol, choline chloride-oxalic acid and choline chloride-urea) were developed for extracting protein from SSM and compared with alkaline. Result indicated that alkaline could effectively extract 56.9% protein from SSM and its protein content was 73.1%, higher than DES at 31.0%-41.4% and 64.3%-67.5%, respectively. However, compared to alkali, DES led to a product with less β-sheet, more β-turn, more essential amino acids, higher total amino acid content, especially choline chloride-urea which extracted protein showing an integrated and similar protein weight distribution compared to SSM. Also, this protein extracted chloride-urea showed a highest digestibility in vitro(by pepsin)(54.2%). These results indicated that choline chloride-urea extraction is better than alkaline extraction for SSM.

Prediction of Landscape Pattern of Soft Sandstone Area (SSA) after Seabuckthorn Planting

As a pioneer plant in the gully slopes in the Soft Sandstone Area (SSA) for eco-economical consideration, ten years (1999-2008) planting of seabuckthorn has made 1642.83 km2, or 9.84%, of the total area of SSA change into seabuckthorn coverage. In SSA the landscape has been divided into 9 types, such as seabuckthorn, sand, water, settlement, bush, open vegetation, forest, grassland and unused land. Seabuckthorn type is separated from the bush type for estimating the role of seabuckthron planting. By means of the Markov model, the developing trends of every landscape types can be determined to support the seabuckthorn project which influences the landscape pattern deeply in SSA. The prediction shows that the optimism ratio of seabuckthorn in the future should be 10.21%, the open vegetation 32.25%, and the forest percentage under 10%, which is a very wise tactics to avoid the serious death of various vegetations in SSA to match the local arid eco-environment.

Evaluation on Environmental Resource Value of Artificial Seabuckthorn Forest in Sandstone Area during 1999-2008

China Seabuckthorn Ecological Project has been implemented for a decade (from 1999 to 2008) with an increasing seabuckthorn area of 1642.83 km2 in?the Sandstone Areas of Shanxi, Shaanxi and Inner Mongolia—the most serious soil erosion area in China. The vast artificial seabuckthorn forest’s value in environmental resource is assessed via 7 indicators on first-level, 15 indicators on secondary level and 35 indicators on third level in this research. As of 2008, the seabuckthorn environmental resource value is 10.017 billion RMB Yuan, which equals to 37.1 times of the project’s total investment of 0.27 billion RMB Yuan. It is proposed in this research that the external economical values such as soil and water conservation, windbreak and sand-fixation can be realized through relative national policies and taxes. Carbon-fixation and oxygen-producing value can be achieved through the international carbon trade.

Evaluation of nutrient value of seabuckthorn in north China

五种矿物质或元素，粗略的蛋白质，离氨酸， methionine+cystine，钙和磷，为 seabuckthorn 的食物营养价值的评估被选择(Hippophae rhamnoides ssp。sinensis ) 用鈥渇o od 分析鈥的方法？seabuckthorn 种子，叶子和水果残余的决定价值(未 i ) 在粗饲料的 16 种类型之中在第五，第六和第九个位置评价。种子，叶子和 seabuckthorn 的水果残余对家畜和家禽粗饲料合适。家畜和家禽的重量更加在用 seabuckthorn 喂以后被增加。seabuckthorn 的发展应该在北方中国为家畜和家禽作为食物，食物前和食品添加剂有大潜力。处理设备应该对 seabuckthorn 食物发展方法和策略给予更多的注意， seabuckthorn 粗饲料工业应该被支持。关键词 seabuckthorn - 食物分析 - 评估 -

Extracts from Chinese Seabuckthorn Berry Have Antimicrobial Activity in vitro

Chinese seabuckthorn berry contains functional components. The berry was extracted by ethanol-water solution. MTT assay and growth rate method were applied to determine antibacterial ability in vitro of the extract solution. The antibacterial and antifungal assay indicated that the extract exhibited different degree of inhibitory activity. The result showed that the extract at a concentration of 1 250 mg/L had the maximum inhibitory effect against Escherichia coli, Bacillus subtilis, and Staphylococcus aureus. Inhibitory effect against B. subtilis was much larger than that against the other two bacterial species. MIC values(minimum inhibitory concentration) of the extract on three bacterial species were 5 000, 1 250, and 1 000 mg/L respectively. IC_(50)(50% inhibiting concentration) evaluation of Trichoderma viride, Rhizopus stolonifer, Aspergillus niger, Penicillium expansum, and Vintage Red indicated that the berry had the strongest inhibitory effect against P. expansum(5 520 mg/L), while it had the weakest inhibitory effect against R. stolonifer(18 870 mg/L). The results proved that seabuckthorn berry had highly effective and comprehensive antimicrobial function.

Demarcation of Seabuckthorn Plantations in Three Northern Areas of China

Seabuckthorn (Hippophae rhamnoides) planting areas in the three northern areas (north, northeast and northwest) of China are divided into five planting zones: the semi-humid forest prairie climate zone for ecological and economic types of seabuck- thorn plantations in the southern part of the Loess Plateau; the semi-arid steppe climate zone for similar types of plantations in the central part of the Loess Plateau; the arid desert steppe climate zone for ecological type of seabuckthorn plantations in the northern part of the Loess Plateau; the semi-arid and semi-humid steppe climate zone again for ecological and economic types of plantations in northern Hebei and western Liaoning and the cold humid steppe climate zone for economic types of plantations in the northern part of northeast China. The aim of this demarcation is to avoid a random introduction of seabuckthorn. In each of the five zones, objectives should be set and suitable seabuckthorn species, subspecies and varieties should be planted according to site conditions, seed sources and methods of tree breeding. The cultivation centers, bases, stations, or units should be established and successful models of seedling and planting methods should be encouraged. The principle of matching trees with suitable site conditions and adjusting measures to local conditions should be practiced. From a strategic viewpoint of solving ecological and economic problems of seabuckthorn development in the three northern areas, every seabuckthorn center must have its own germplasm nursery, standard plantation for popularizing, excellent seed and seedling nurseries and sufficient afforestation areas for demonstration and propaganda purposes. These measures would improve the ecological environment and promote economic and social development in the three northern areas of China.

Comparative quantitative analysis of fruit oil from Hippophae rhamnoides(seabuckthorn) by qNMR, FTIR and GC–MS

Objective: To develop a qNMR method for quantitative analysis of triacylglycerols in fruit oil of Hippophae rhamnoides(seabuckthorn, SBT) and analyze commercial samples of SBT oils using GC–MS and FTIR.Methods: SBT fruit oil(IPHRFH) was extracted with hexane and the triglyceride(TAG) was isolated by vacuum liquid chromatography. Six different branded SBT oils purchased from e-commerce suppliers(Amazon) and in-house prepared SBT oil was analyzed by qNMR and fatty acyl composition of TAGs determined by using NMR. In-house oil was also analysed by GC–MS and FTIR spectroscopy.Results: The qNMR results showed that the oil contained 80.3% of triacylglycerol(TAG). The SBT oil TAGs comprised of linolenate 6.6%, palmitoleate/oleate 65.4%, and total saturated fatty acyl chain including palmitate 28% as determined by qNMR. GC–MS analysis revealed that the major acyl functionalities present in the TAG were palmitoleic acid 36.5%, oleic acid 12.9%, palmitic acid 21.2%, and linoleic acid 18%. Of the six commercial samples analyzed, samples from only one supplier(SW) were fruit oil;All others were the seed oils or mix of fruit oil and seed oil. The labels for samples except for the SW did not indicate whether it was fruit oil or seed oil.Conclusion: The results suggest that SBT oil should be analyzed by combination of GC–MS, FTIR and qNMR for factual content of free fatty acid or TAGs, which are chemically different in nature and affect the quality of oil. GC–MS showed the content of omega free fatty acids after hydrolysis, while qNMR and FTIR showed the content of TAGs. The major acyl functionalities found in SBT fruit oil TAGs are palmitoleate/palmitate/oleate, while linoleate and linonelate make up a minor fraction. Furthermore,analysis of commercial samples showed discrepancies between label claims and actual content.

沙棘枝杆力学特性试验研究

为获取沙棘枝杆的力学参数,支撑沙棘收获设备的开发,以新疆大果沙棘枝杆为对象,采用万能试验机对其剪切、弯曲和压缩的力学性能进行了测定,并在此基础上评估了直径和含水率对沙棘枝杆力学性能的影响。力学试验研究表明:沙棘枝杆的抗弯强度随着直径的增大而增大,直径为20mm时最大,为78.14MPa,直径为10mm时最小,为67.14MPa;抗剪强度会随着直径的增大先略微减小再增大,直径为20mm时最大,为45.46MPa,直径为12.5mm时最小,为30.90MPa;抗压强度会随着直径的增大而增大,直径为20mm时最大,为30.76MPa,直径为10mm时最小,为22.79MPa;弯曲试验和压缩试验中极限承载力随着含水率的上升而下降,压缩试验的趋势更为显著;剪切试验的极限承载力开始时随着含水率的上升而上升,在30%时到达最大值,之后随着含水率的上升而下降。研究结果可为沙棘枝杆的收获、破碎和加工设备的设计提供参数依据。

沙棘果食品开发利用研究进展与发展对策

沙棘富含多种生物活性成分,具有药食同源特性,其果实、种子、果皮、枝叶均可被加工利用。综述了沙棘果的营养保健价值、产品形式和加工流程,分析了近年来沙棘果产业的主要问题,并提出一些建议,旨在为沙棘果相关食品产业化发展提供参考依据。

沙棘果不同极性部位体外及体内抗氧化作用研究

目的 探究沙棘果不同极性部位体外抗氧化能力及体内抗氧化作用,明确有效部位,为拓展沙棘果研发利用、优化制备工艺提供依据。方法 以沙棘果为原料,95%乙醇提取后依次用石油醚、乙酸乙酯、正丁醇进行萃取,得到不同极性部位提取物,采用清除1,1-二苯基-2-三硝基苯肼自由基(DPPH·)法、超氧阴离子自由基(O_(2)·^(-))法及羟自由基(·OH)法,比较不同极性部位体外抗氧化作用。将110只小鼠随机分为空白组(Control),模型组(Model),沙棘果乙酸乙酯层低剂量组(YD,0.015 g/kg)、沙棘果乙酸乙酯层中剂量组(YZ,0.045 g/kg)、沙棘果乙酸乙酯层高剂量组(YG,0.135 g/kg),沙棘果正丁醇层低剂量组(ZD,0.04 g/kg)、沙棘果正丁醇层中剂量组(ZZ,0.13 g/kg)、沙棘果正丁醇层高剂量组(ZG,0.39 g/kg)及沙棘果醇提取物低剂量组(SD,0.16 g/kg)、沙棘果醇提取物中剂量组(SZ,0.5 g/kg)、沙棘果醇提取物高剂量组(SG,1.5 g/kg),每组10只。除Control外,其余各组每日腹腔注射浓度为0.05 g/mL D-半乳糖(D-gal)溶液,剂量为10 g/0.1 mL,Control注入等量生理盐水,连续60 d。造模第11天开始灌胃给药,给药组分别灌胃沙棘果不同极性部位提取物低、中、高剂量,Control与Model灌胃蒸馏水。末次给药后,考察小鼠体质量变化、脏器指数,对比各组小鼠的血清抗氧化能力,包括血清总抗氧化能力(total antioxidant capacity,T-AOC)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-PX)、超氧化物歧化酶(superoxide dismutase,SOD)以及丙二醛(malondialdehyde,MDA)水平,观察心脏、肺脏、肾脏的病理变化。结果 沙棘果不同极性部位均可清除DPPH·、O_(2)·~-、·OH,具有体外抗氧化活性,其中沙棘果醇提取部位对DPPH·清除率高达76.09%,清除效果最强;体内实验结果显示,YG、ZZ、ZG、SZ、SG血清GSH-PX、SOD、T-AOC水平增高(P<0.05),且均可抑制MDA的积累(P<0.01)。其中SG血清GSH-PX抗氧化水平为(872.73±165.39)U/mL、SOD为(180.02±12.33)U/mL、T-AOC为(9.25±1.45)U/mL,高于Model(P<0.01),MDA水平为(4.93±2.07)nmol/mL,相比于Model有所下降(P<0.05)。HE染色结果表明,沙棘果不同极性部位对心脏、肺脏、肾脏组织细胞及结构损伤具有较好的修复作用。结论 沙棘果不同极性部位均具有一定的抗氧化作用,其中沙棘果醇提取物抗氧化能力最强,其余各部位抗氧化能力与极性呈正相关。

砒砂岩区十大孔兑沙棘生态减沙工程现状调查及管理对策研究

砒砂岩区沙棘生态减沙工程是我国晋陕蒙区防风治沙、强化水土保持的重要举措,该工程的发展现状及管理对策值得引起特别关注。本文以十大孔兑上游砒砂岩丘陵沟壑区沙棘林为研究对象,通过实地调研与问卷调查等研究方法,明确砒砂岩区十大孔兑沙棘生态减沙工程发展现状、实施现状、沙棘林生长状况、抚育措施及其经济效益等情况,提出了统一筹划构建沙棘林长效管理机制、多举并措健全沙棘林抚育措施实施方案、因地制宜完善沙棘资源产业化开发方案等管理对策,为晋陕蒙区砒砂岩区十大孔兑沙棘生态减沙工程的实施与管理提供科学支撑。

Seabuckthorn Berries Extract Attenuates Pulmonary Vascular Hyperpermeability in Lipopolysaccharide-Induced Acute Lung Injury in Mice

Objective:To investigate the effect of seabuckthorn berries extract(SBE)on pulmonary vascular hyperpermeability in the mice model of acute lung injury(ALI)induced by lipopolysaccharide(LPS).Methods:Sixty Kunming mice were allocated into 6 groups by a random number table,including control,LPS,dexamethasone(Dex,1 mg/kg),and 120,240 and 480 mg/kg SBE groups,10 mice in each group.Except the control group,mice were pre-treated with Dex and SBE,respectively,for 7 days before LPS was intraperitoneally injected to induce ALl.Pulmonary vascular hyperpermeabilitywas evaluated by histopathologic observation and transvascular leakagedetermination.Tumor necrosis factor alpha(TNF-α)and interleukin-6(IL-6)levels in serum were measured using enzyme-linked immunosorbent assay.The expression of nuclear factor-kappa B(NF-k B)p65 in lung cells was determined by immunofluorescence analysis.The contents of cytoplasmic inhibitorof nuclear factor-kBkinase(IKK)and nuclearp65,as wellasdownstreamproteins of E-selectin(CD62E)and intercellular adhesion molecule-1(ICAM-1),were determined using Western blot analysis.Results:Histopathological observation confirmed SBEtreatment alleviatedmorphological lesion induced by LPS.Compared with the LPS group,480 mg/kg SBE significantly decreased the water content of lung,Evans blue accumulation in lung tissue,and protein concentration and neutrophils count in bronchoalveolar lavage fluid(P<o.01);moreover,480 mg/kg SBE significantly suppressed release of TNF-αand IL-6,and down-regulated expressions of IKK,nuclear p65,ICAM-1 and CD62E(P<0.01).Conclusion:SBE maintained alveolar-capillary barrier integrity under endotoxin challenge in mice by suppressing the key factors in the pathogenesis of ALl.

沙棘优良杂交种质资源鉴定及筛选

为筛选沙棘优良杂交种质资源,本试验以杂交F_1代及其父母本与对照品种中雌沙棘共13份材料为试材,通过调查比较物候期、生长性状、生长速率及果实性状等相关指标,通过建立隶属函数方程,对10份杂交F_1代材料进行综合评价。结果表明,杂交F_1代果实成熟期为8月13日—15日,介于中国沙棘与蒙古沙棘之间。参试材料平均株高为284.68 cm,平均冠幅为205.85 cm,平均地径为5.47 cm,中蒙杂交沙棘生长性状指标显著高于对照品种中国沙棘雌株与楚伊沙棘,具有树势强,生长速率快的特点。果实百果重平均值为30.33 g,俄罗斯大果沙棘品种楚伊百果重显著高于其他参试材料,中国沙棘雌株百果重最低,为11.21 g。单株产量平均值为8.28 kg,高于母本楚伊沙棘与对照品种中国沙棘雌株,其中中蒙杂雌6材料单株产量最高,为12.25 kg,中国沙棘雌株材料单株产量最低,为2.43 kg。通过综合评价,中蒙杂雌6与中蒙杂雌10综合评价E值高于其他材料,可以作为中熟杂交沙棘种质资源进行保存与推广种植。

基于响应面法优化沙棘普洱茶粉水提工艺及体外抗氧化能力评价研究

以沙棘普洱茶粉水提液中可溶性固形物为响应值,基于单因素及Box-Benhnken响应面法优化实验研究不同料液比、时间、温度、pH的影响.对不同原料配比下的沙棘普洱茶粉进行有效成分检测及体外抗氧化实验,分析、评价其体外抗氧化能力.得到沙棘普洱茶粉(沙棘叶∶普洱茶末=4∶1)的最优水提工艺条件为料液比(原料∶水)为1∶8(g/mL)、提取温度为79℃、提取时间为3 h、pH为8.3.沙棘普洱茶粉的活性成分含量较单一茶粉有所提高,对DPPH(2,2,-diphenyl-1-picrylhydrazyl)、ABTS(2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)、CuPRAC(Cupric ReducingAntioxidant Power)及FRAP(Ferric Reducing Antioxidant Power)均具有较强的清除能力,制备的沙棘普洱茶粉均具有较好的体外抗氧化作用,为沙棘、普洱茶资源利用及功能性茶粉的开发提供一定参考.

不同菌种组合对沙棘酵素发酵过程中理化指标及活性物质变化的影响

目的研究不同菌种组合对沙棘酵素发酵过程中理化指标及活性物质变化的影响。方法以酿酒酵母(Saccharomyces cerevisiae,Sc)、异常汉逊酵母(Hansenulaanomala,Ha)、植物乳植物杆菌(Lactiplantibacillus plantarum,Lp)、Sc+Ha、Sc+Lp、Ha+Lp和Sc+Ha+Lp为发酵菌种,研究不同菌种组合对沙棘酵素发酵过程中pH、总酸、可溶性固性物、乙醇含量等理化指标和总酚、总黄酮、多糖、维生素C(vitamin C,VC)、超氧化物歧化酶(superoxide dismutase,SOD)等活性物质变化的影响。结果不同菌种在发酵过程中对理化指标和活性物质变化趋势的影响基本一致,pH和可溶性固形物含量随发酵时间呈现持续下降的趋势,总酸、总酚、总黄酮、多糖、SOD和还原力等的变化趋势与之相反,乙醇含量先升高后降低,VC含量逐渐降低,发酵后期略有升高。多菌种(Sc+Ha+Lp)发酵沙棘酵素的各指标在发酵过程中尤其是发酵中后期变化最为明显,发酵180 d时,pH和可溶性固形物含量分别下降50.44%和86.61%,总酸、总酚、总黄酮、多糖、SOD和还原力分别升高61.03%、52.14%、91.35%和70.54%、80.45%、65.67%,乙醇含量仅为0.05 g/100 mL,VC含量降低率8.22%;理化指标与活性物质及还原力之间的相关性极显著(P<0.01)。结论多菌种(Sc+Ha+Lp)发酵可提升沙棘酵素产品营养价值,乳酸菌与酵母菌之间的代谢产物互补机制,可能在促进微生物共生、提高小分子活性物质含量和改进风味品质等方面起着重要的作用。

复方沙棘籽油加速大鼠肛周脓肿术后创面修复的作用机制研究

目的:探讨复方沙棘籽油加速大鼠肛周脓肿术后创面修复的作用机制。方法:取制备成功肛周脓肿术后创面SD模型鼠共50只,随机分为5组,分别为模型组(G1,给予蒸馏水);阳性对照组(G2,给予高锰酸钾);复方沙棘籽油纳米乳化液低剂量治疗组(G3);复方沙棘籽油纳米乳化液中剂量治疗组(G4);复方沙棘籽油纳米乳化液高剂量治疗组(G5);造模后各组实验大鼠创面用相应实验药物均匀涂抹,2次/d,连续给药14 d。观察治疗5 d、8 d、14 d后各组大鼠创面愈合情况,计算各组大鼠创面愈合率,分别取各组大鼠创面组织,应用免疫组化S-P法检测创面表皮生长因子(EGF)、磷酸化细胞外调节蛋白激酶(ERK)1/2、白细胞介素-8(IL-8)、前列腺素E2(PGE2)表达情况。结果:创面处理后5 d、8 d、14 d,G5组、G4组、G3组、G2组、G1组大鼠创面愈合率依次升高(均P<0.05)。术后14 d,大鼠创面IL-8表达水平为G2组<G5组<G4组<G3组<G1组(P<0.05);PEG2表达水平为G5组<G2组<G4组<G3组<G1组(P<0.05)。术后14 d,大鼠创面EGF、ERK1/2表达为G5组>G4组>G3组>G2组>G1组(均P<0.05)。结论:复方沙棘籽油纳米乳化液可有效促进大鼠肛周脓肿术后创面愈合,这可能与IL-8及PEG2炎症介质有关,还可能与EGF-ERK通路活化促进创面角质形成细胞、成纤维细胞生长、增殖、分化有关。

沙棘果渣多糖-纳米银复合粒子的制备及其抑菌活性

该文以沙棘果渣多糖和硝酸银为材料,研究沙棘果渣多糖-纳米银的制备工艺,并探究其抑菌活性。结果表明,反应温度35℃、pH9、硝酸银和沙棘果渣多糖体积比为1.0∶1、反应24 h后紫外照射60 min条件下制备的沙棘果渣多糖-纳米银粒径最小,为31.77 nm,且该复合纳米颗粒对大肠杆菌和白色葡萄球菌的生长均具有较强的抑制作用,最小抑菌浓度分别为4μg/mL和6μg/mL。

沙棘干乳剂联合双歧杆菌四联活菌片治疗儿童功能性消化不良的效果

目的:探讨沙棘干乳剂联合双歧杆菌四联活菌片治疗儿童功能性消化不良(FD)的效果。方法:选取2021年1月至2022年12月该院诊治的68例FD患儿进行前瞻性研究,根据随机数字表法将其分为观察组、对照组各34例。对照组口服双歧杆菌四联活菌片治疗,观察组在对照组基础上联合沙棘干乳剂治疗,两组均持续治疗2周。比较两组临床疗效、治疗前后症状评分、血清神经生化指标[降钙素基因相关肽(CGRP)、促肾上腺素皮质激素释放因子(CRF)]水平、胃动力指标[胃窦收缩频率(ACF)、胃窦收缩幅度(ACA)、运动指数(MI)]水平及不良反应发生率。结果:观察组治疗总有效率为94.12%(32/34),高于对照组的73.53%(25/34),差异有统计学意义(P<0.05);治疗后,观察组消化不良、腹胀症状评分及血清CGRP、CRF水平均低于对照组,ACF、MI、ACA值均高于对照组,差异有统计学意义(P<0.05);两组不良反应发生率比较,差异无统计学意义(P>0.05)。结论:沙棘干乳剂联合双歧杆菌四联活菌片治疗FD患儿效果显著,可有效改善临床症状,降低CGRP、CRF水平,增强胃肠动力,安全性良好,效果优于单用双歧杆菌四联活菌片治疗。

沙棘黄酮对肉兔生长性能、屠宰性能、血清生化指标、抗氧化能力和免疫功能的影响

本试验旨在研究沙棘黄酮对肉兔生长性能、屠宰性能、血清生化指标、抗氧化能力和免疫功能的影响。选取(35±2)日龄、体重相近、健康状况良好的伊拉肉兔120只,随机分为5组,每组6个重复,每个重复4只兔(公母各占1/2)。对照组饲喂基础饲粮,试验组在基础饲粮中分别添加0.01%、0.02%、0.03%和0.04%的沙棘黄酮。预试期7 d,正试期35 d。结果表明:1)与对照组相比,0.02%沙棘黄酮组末重、平均日增重和平均日采食量显著提高(P<0.05),料重比显著降低(P<0.05);0.02%、0.03%和0.04%沙棘黄酮组肉兔腹泻率显著降低(P<0.05)。2)与对照组相比,0.02%沙棘黄酮组屠宰率显著提高(P<0.05),0.01%和0.02%沙棘黄酮组半净膛率和全净膛率显著提高(P<0.05)。3)与对照组相比,0.03%沙棘黄酮组血清总胆固醇、低密度脂蛋白和葡萄糖含量显著降低(P<0.05),0.02%、0.03%和0.04%沙棘黄酮组血清高密度脂蛋白含量显著提高(P<0.05)。4)与对照组相比,0.02%沙棘黄酮组血清和肝脏超氧化物歧化酶活性、总抗氧化能力显著提高(P<0.05),0.02%、0.03%和0.04%沙棘黄酮组血清和肝脏丙二醛含量显著降低(P<0.05)。5)与对照组相比,0.02%沙棘黄酮组胸腺指数和血清免疫球蛋白A、免疫球蛋白G、免疫球蛋白M、白细胞介素-2、白细胞介素-6含量显著提高(P<0.05),0.03%沙棘黄酮组脾脏指数和血清免疫球蛋白G、免疫球蛋白M、白细胞介素-2、白细胞介素-6含量显著提高(P<0.05)。综上所述,饲粮中添加沙棘黄酮能够提高肉兔生长性能,调控胆固醇的代谢,提高高密度脂蛋白和免疫因子含量,提高抗氧化能力,增强免疫功能。本试验条件下,饲粮中沙棘黄酮适宜添加水平为0.02%。