A father worked as an accountant for the Air National Guard.Despite a regular adequate income hehad a “poor” mindset,always unhappy about money matters and very veal about it.One year at an air show,in a crowd of ma...A father worked as an accountant for the Air National Guard.Despite a regular adequate income hehad a “poor” mindset,always unhappy about money matters and very veal about it.One year at an air show,in a crowd of many close-by people,his daughter looked up at him andsaid,“Dad,what do you do out here?”展开更多
Good toilet hygiene can reduce the risk of pathogenic transmission.During flushing,pathogenic bacteria can be spread by the water droplets,especially in the toilet seat.Escherichia coli(E.coli)is one of the frequently...Good toilet hygiene can reduce the risk of pathogenic transmission.During flushing,pathogenic bacteria can be spread by the water droplets,especially in the toilet seat.Escherichia coli(E.coli)is one of the frequently occurring Gram-negative bacterial pathogens found in the toilet seats.In this study,we investigated the potential anti-E.coli activity of some azobenzene compounds.Among them,compound 2,with the heterocyclic nitrogen in both phenyl rings,showed the strongest antibacterial effect on E.coli,with the minimum inhibitory concentration of 80μg/mL and the minimum bactericidal concentration of 320μg/mL.Compound 2 displayed a comparative zone of clearance[(14.50±2.29)mm]to that of ampicillin[(13.17±1.76)mm].The reactive oxygen species(ROS)assay suggested that compound 2 might induce the loss of bacteria via the oxidative stress by eliciting the generation of intracellular ROS.We further examined the potential cytotoxicity of compound 2 to the human skin HaCaT cells.Compound 2-treated skin cells showed a good maintenance of cellular morphology and a clear definition of cell nuclei,with a satisfactory cell viability(ca.90%)at 80μg/mL.Overall,compound 2 would be a possible antibacterial coating agent for the surface sanitizer of the toilet seats to inhibit the growth of E.coli.展开更多
文摘A father worked as an accountant for the Air National Guard.Despite a regular adequate income hehad a “poor” mindset,always unhappy about money matters and very veal about it.One year at an air show,in a crowd of many close-by people,his daughter looked up at him andsaid,“Dad,what do you do out here?”
基金WONG Wai-Yeung thanks the ITC Guangdong-Hong Kong Technology Cooperation Funding Scheme(TCFS)(No.GHP/038/19GD)and Chengdu University for the financial support.CHUI Chung-Hin from One Health International Limited acknowledges the support of Research Talent Hub Ref.:RtH/016/20,Innovation and Technology Commission,Hong Kong,China.
文摘Good toilet hygiene can reduce the risk of pathogenic transmission.During flushing,pathogenic bacteria can be spread by the water droplets,especially in the toilet seat.Escherichia coli(E.coli)is one of the frequently occurring Gram-negative bacterial pathogens found in the toilet seats.In this study,we investigated the potential anti-E.coli activity of some azobenzene compounds.Among them,compound 2,with the heterocyclic nitrogen in both phenyl rings,showed the strongest antibacterial effect on E.coli,with the minimum inhibitory concentration of 80μg/mL and the minimum bactericidal concentration of 320μg/mL.Compound 2 displayed a comparative zone of clearance[(14.50±2.29)mm]to that of ampicillin[(13.17±1.76)mm].The reactive oxygen species(ROS)assay suggested that compound 2 might induce the loss of bacteria via the oxidative stress by eliciting the generation of intracellular ROS.We further examined the potential cytotoxicity of compound 2 to the human skin HaCaT cells.Compound 2-treated skin cells showed a good maintenance of cellular morphology and a clear definition of cell nuclei,with a satisfactory cell viability(ca.90%)at 80μg/mL.Overall,compound 2 would be a possible antibacterial coating agent for the surface sanitizer of the toilet seats to inhibit the growth of E.coli.
