Optical second-harmonic generation imaging for identifying gastrointestinal stromal tumors

Gastrointestinal stromal tumors(GISTs)are the most common mesenchymal tumors arising in the digest tract.It brings a challenge to diagnosis because it is asymptomatic clinically.It is well known that tumor development is often accompanied by the changes in the morphology of collagen fibers.Nowadays,an emerging optical imaging technique,second-harmonic generation(SHG),can directly identify collagen fibers without staining due to its noncentrosymmetric properties.Therefore,in this study,we attempt to assess the feasibility of SHG imaging for detecting GISTs by monitoring the morphological changes of collagen fibers in tumor microenvironment.We found that collagen alterations occurred obviously in the GISTs by comparing with normal tissues,and furthermore,two morphological features from SHG images were extracted to quantitatively assess the morphological difference of collagen fibers between normal muscular layer and GISTs by means of automated image analysis.Quantitative analyses show a significant difference in the two collagen features.This study demonstrates the potential of SHG imaging as an adjunctive diagnostic tool for label-free identification of GISTs.

An Enhanced GAN for Image Generation

Generative adversarial networks(GANs)with gaming abilities have been widely applied in image generation.However,gamistic generators and discriminators may reduce the robustness of the obtained GANs in image generation under varying scenes.Enhancing the relation of hierarchical information in a generation network and enlarging differences of different network architectures can facilitate more structural information to improve the generation effect for image generation.In this paper,we propose an enhanced GAN via improving a generator for image generation(EIGGAN).EIGGAN applies a spatial attention to a generator to extract salient information to enhance the truthfulness of the generated images.Taking into relation the context account,parallel residual operations are fused into a generation network to extract more structural information from the different layers.Finally,a mixed loss function in a GAN is exploited to make a tradeoff between speed and accuracy to generate more realistic images.Experimental results show that the proposed method is superior to popular methods,i.e.,Wasserstein GAN with gradient penalty(WGAN-GP)in terms of many indexes,i.e.,Frechet Inception Distance,Learned Perceptual Image Patch Similarity,Multi-Scale Structural Similarity Index Measure,Kernel Inception Distance,Number of Statistically-Different Bins,Inception Score and some visual images for image generation.

Imaging the crystal orientation of 2D transition metal dichalcogenides using polarization-resolved second-harmonic generation

We use laser-scanning nonlinear imaging microscopy in atomically thin transition metal dichalcogenides(TMDs)to reveal information on the crystalline orientation distribution,within the 2D lattice.In particular,we perform polarization-resolved second-harmonic generation(PSHG)imaging in a stationary,raster-scanned chemical vapor deposition(CVD)-grown WS2 flake,in order to obtain with high precision a spatially resolved map of the orientation of its main crystallographic axis(armchair orientation).By fitting the experimental PSHG images of sub-micron resolution into a generalized nonlinear model,we are able to determine the armchair orientation for every pixel of the image of the 2D material,with further improved resolution.This pixel-wise mapping of the armchair orientation of 2D WS2 allows us to distinguish between different domains,reveal fine structure,and estimate the crystal orientation variability,which can be used as a unique crystal quality marker over large areas.The necessity and superiority of a polarization-resolved analysis over intensity-only measurements is experimentally demonstrated,while the advantages of PSHG over other techniques are analysed and discussed.

Third-harmonic generation and imaging with resonant Si membrane metasurface

Dielectric metasurfaces play an increasingly important role in enhancing optical nonlinear generations owing to their ability to support strong light-matter interactions based on Mie-type multipolar resonances.Compared to metasurfaces composed of the periodic arrangement of nanoparticles,inverse,so-called,membrane metasurfaces offer unique possibilities for supporting multipolar resonances,while maintaining small unit cell size,large mode volume and high field enhancement for enhancing nonlinear frequency conversion.Here,we theoretically and experimentally investigate the formation of bound states in the continuum(BICs)from silicon dimer-hole membrane metasurfaces.We demonstrate that our BIC-formed resonance features a strong and tailorable electric near-field confinement inside the silicon membrane films.Furthermore,we show that by tuning the gap between the holes,one can open a leaky channel to transform these regular BICs into quasi-BICs,which can be excited directly under normal plane wave incidence.To prove the capabilities of such metasurfaces,we demonstrate the conversion of an infrared image to the visible range,based on the Third-harmonic generation(THG)process with the resonant membrane metasurfaces.Our results suggest a new paradigm for realising efficient nonlinear photonics metadevices and hold promise for extending the applications of nonlinear structuring surfaces to new types of all-optical near-infrared imaging technologies.

Broadband and continuous wave pumped second-harmonic generation from microfiber coated with layered GaSe crystal

The conversion-efficiency for second-harmonic(SH)in optical fibers is significantly limited by extremely weak second-order nonlinearity of fused silica,and pulse pump lasers with high peak power are widely employed.Here,we propose a simple strategy to efficiently realize the broadband and continuous wave(CW)pumped SH,by transferring a crystalline GaSe coating onto a microfiber with phase-matching diameter.In the experiment,high efficiency up to 0.08%W-1mm-1 is reached for a C-band pump laser.The high enough efficiency not only guarantees SH at a single frequency pumped by a CW laser,but also multi-frequencies mixing supported by three CW light sources.Moreover,broadband SH spectrum is also achieved under the pump of a superluminescent light-emitting diode source with a 79.3 nm bandwidth.The proposed scheme provides a beneficial method to the enhancement of various nonlinear parameter processes,development of quasi-monochromatic or broadband CW light sources at new wavelength regions.

Resonantly enhanced second-and third-harmonic generation in dielectric nonlinear metasurfaces

Nonlinear dielectric metasurfaces provide a promising approach to control and manipulate frequency conversion optical processes at the nanoscale,thus facilitating both advances in fundamental research and the development of new practical applications in photonics,lasing,and sensing.Here,we employ symmetry-broken metasurfaces made of centrosymmetric amorphous silicon for resonantly enhanced second-and third-order nonlinear optical response.Exploiting the rich physics of optical quasi-bound states in the continuum and guided mode resonances,we comprehensively study through rigorous numerical calculations the relative contribution of surface and bulk effects to second-harmonic generation(SHG)and the bulk contribution to third-harmonic generation(THG) from the meta-atoms.Next,we experimentally achieve optical resonances with high quality factors,which greatly boosts light-matter interaction,resulting in about 550 times SHG enhancement and nearly 5000-fold increase of THG.A good agreement between theoretical predictions and experimental measurements is observed.To gain deeper insights into the physics of the investigated nonlinear optical processes,we further numerically study the relation between nonlinear emission and the structural asymmetry of the metasurface and reveal that the generated harmonic signals arising from linear sharp resonances are highly dependent on the asymmetry of the meta-atoms.Our work suggests a fruitful strategy to enhance the harmonic generation and effectively control different orders of harmonics in all-dielectric metasurfaces,enabling the development of efficient active photonic nanodevices.

In vivo label-free measurement of blood flow velocity symmetry based on dual line scanning third-harmonic generation microscopy excited at the 1700 nm window

Measurement of bloodflow velocity is key to understanding physiology and pathology in vivo.While most measurements are performed at the middle of the blood vessel,little research has been done on characterizing the instantaneous bloodflow velocity distribution.This is mainly due to the lack of measurement technology with high spatial and temporal resolution.Here,we tackle this problem with our recently developed dual-wavelength line-scan third-harmonic generation(THG)imaging technology.Simultaneous acquisition of dual-wavelength THG line-scanning signals enables measurement of bloodflow velocities at two radially symmetric positions in both venules and arterioles in mouse brain in vivo.Our results clearly show that the instantaneous bloodflow velocity is not symmetric under general conditions.

Remote Sensing Image Encryption Using Optimal Key Generation-Based Chaotic Encryption

The Internet of Things(IoT)offers a new era of connectivity,which goes beyond laptops and smart connected devices for connected vehicles,smart homes,smart cities,and connected healthcare.The massive quantity of data gathered from numerous IoT devices poses security and privacy concerns for users.With the increasing use of multimedia in communications,the content security of remote-sensing images attracted much attention in academia and industry.Image encryption is important for securing remote sensing images in the IoT environment.Recently,researchers have introduced plenty of algorithms for encrypting images.This study introduces an Improved Sine Cosine Algorithm with Chaotic Encryption based Remote Sensing Image Encryption(ISCACE-RSI)technique in IoT Environment.The proposed model follows a three-stage process,namely pre-processing,encryption,and optimal key generation.The remote sensing images were preprocessed at the initial stage to enhance the image quality.Next,the ISCACERSI technique exploits the double-layer remote sensing image encryption(DLRSIE)algorithm for encrypting the images.The DLRSIE methodology incorporates the design of Chaotic Maps and deoxyribonucleic acid(DNA)Strand Displacement(DNASD)approach.The chaotic map is employed for generating pseudorandom sequences and implementing routine scrambling and diffusion processes on the plaintext images.Then,the study presents three DNASD-related encryption rules based on the variety of DNASD,and those rules are applied for encrypting the images at the DNA sequence level.For an optimal key generation of the DLRSIE technique,the ISCA is applied with an objective function of the maximization of peak signal to noise ratio(PSNR).To examine the performance of the ISCACE-RSI model,a detailed set of simulations were conducted.The comparative study reported the better performance of the ISCACE-RSI model over other existing approaches.

Time-domain analysis of second-harmonic generation of primary Lamb wave propagation in an elastic plate

Within the second-order perturbation approximation, this paper investigates the physical process of generation of the time-domain second harmonic by a primary Lamb wave waveform in an elastic plate. The present work is performed based on the preconditions that the phase velocity matching is satisfied and that the transfer of energy from the primary Lamb wave to the double frequency Lamb wave is not zero. It investigates the influences of the difference between the group velocities of the primary Lamb wave and the double frequency Lamb wave, the propagation distance and the duration of the primary Lamb wave waveform on the envelope shape of the time-domain second harmonic. It finds that the maximum magnitude of the envelope of the second-harmonic waveform can grow within some propagation distance even if the condition of group velocity matching is not satisfied. Our analyses also indicate that the maximum magnitude of the envelope of the second-harmonic waveform is kept constant beyond a specific propagation distance. Furthermore, it concludes that the integration amplitude of the time-domain second-harmonic waveform always grows with propagation distance within the second-order perturbation. The present research yields new physical insight not previously available into the effect of generation of the time-domain second harmonic by propagation of a primary Lamb wave waveform.

Numerical Perspective of Second-Harmonic Generation of Circumferential Guided Wave Propagation in a Circular Tube

The effect of second-harmonic generation （SHG） by primary （fundamental） circumferential guided wave （CGW） propagation is investigated from a numerical standpoint. To enable that the second harmonic of the primary CGW mode can accumulate along the circumferential direction, an appropriate mode pair of primary and double frequency CGWs is chosen. Finite element simulations and evaluations of nonlinear CGW propagation are analyzed for the selected CGW mode pair. The numerical simulations performed directly demonstrate that the response of SHG is completely generated by the desired primary CGW mode that satisfies the condition of phase velocity matching at a specific driving frequency, and that the second harmonic of the primary CGW mode does have a cumulative effect with circumferential angles. The numerical perspective obtained yields an insight into the complicated physical process of SHG of primary CGW propagation unavailable previously.

INFINITELY MANY SOLITARY WAVES DUE TO THE SECOND-HARMONIC GENERATION IN QUADRATIC MEDIA

In this paper, we consider the following coupled Schrodinger system with χ^(2) nonlinearities {-Δu1+V1(x)u1=αu1u2,x∈R^N,-Δu2+V2(x)u2=α/2u^21+βu^22,x∈R^N.which arises from second-harmonic generation in quadratic media. Here V1(x) and V2(x) are radially positive functions, 2 ≤ N < 6, α > 0 and α > β. Assume that the potential functions V1(x) and V2(x) satisfy some algebraic decay at infinity. Applying the finite dimensional reduction method, we construct an unbounded sequence of non-radial vector solutions of synchronized type.

Enhancement effect of cumulative second-harmonic generation by closed propagation feature of circumferential guided waves

On the basis of second-order perturbation approximate and modal expansion approach,we investigate the enhancement effect of cumulative second-harmonic generation(SHG)of circumferential guided waves(CGWs)in a circular tube,which is inherently induced by the closed propagation feature of CGWs.An appropriate mode pair of primary-and double-frequency CGWs satisfying the phase velocity matching and nonzero energy flux is selected to ensure that the second harmonic generated by primary CGW propagation can accumulate along the circumference.Using a coherent superposition of multi-waves,a model of unidirectional CGW propagation is established for analyzing the enhancement effect of cumulative SHG of primary CGW mode selected.The theoretical analyses and numerical simulations performed directly demonstrate that the second harmonic generated does have a cumulative effect along the circumferential direction and the closed propagation feature of CGWs does enhance the magnitude of cumulative second harmonic generated.Potential applications of the enhancement effect of cumulative SHG of CGWs are considered and discussed.The theoretical analysis and numerical simulation perspective presented here yield an insight previously unavailable into the physical mechanism of the enhancement effect of cumulative SHG by closed propagation feature of CGWs in a circular tube.

Analysis of Second-Harmonic Generation of Low-Frequency Dilatational Lamb Waves in a Two-Layered Composite Plate

We analyze the effect of second-harmonic generation（SHG） of primary Lamb wave propagation in a two-layered composite plate, and then investigate the influence of interfacial properties on the said effect at low frequency. It is found that changes in the interfacial properties essentially affect the dispersion relation and then the maximum cumulative distance of the double-frequency Lamb wave generated. This will remarkably influence the efficiency of SHG. To overcome the complications arising from the inherent dispersion and multimode natures in analyzing the SHG effect of Lamb waves, the present work focuses on the analysis of the SHG effect of low-frequency dilatational Lamb wave propagation. Both the numerical analysis and finite element simulation indicate that the SHG effect of low-frequency dilatational Lamb wave propagation is found to be much more sensitive to changes in the interfacial properties than primary Lamb waves. The potential of using the SHG effect of low-frequency dilatational Lamb waves to characterize a minor change in the interfacial properties is analyzed.

Experimental Observation of Cumulative Second-Harmonic Generation of Circumferential Guided Wave Propagation in a Circular Tube

The experimental observation of cumulative second-harmonic generation of the primary circumferential guided wave propagation is reported. A pair of wedge transducers is used to generate the primary circumferential guided wave desired and to detect its fundamental-frequency and second-harmonic amplitudes on the outside surface of the circular tube. The amplitudes of the fundamental waves and the second harmonics of the circumferential guided wave propagation are measured for different separations between the two wedge transducers. At the driving frequency where the primary and the double-frequency circumferential guided waves have the same linear phase velocities, the clear second-harmonic signals can be observed. The quantitative relationships between the second-harmonic amplitudes and circumferential angle are analyzed. It is experimentally verified that the second harmonics of primary circumferential guided waves do have a cumulative growth effect with the circumferential angle.

Review on second-harmonic generation of ultrasonic guided waves in solid media(Ⅰ): Theoretical analyses

Considering the high sensitivity of the nonlinear ultrasonic measurement technique and great advantages of the guided wave testing method, the use of nonlinear ultrasonic guided waves provides a promising means for evaluating and characterizing the hidden and/or inaccessible damage/degradation in solid media. Increasing attention on the development of the testing method based on nonlinear ultrasonic guided waves is largely attributed to the theoretical advances of nonlinear guided waves propagation in solid media. One of the typical acoustic nonlinear responses is the generation of second harmonics that can be used to effectively evaluate damage/degradation in materials/structures. In this paper, the theoretical progress of second-harmonic generation（SHG） of ultrasonic guided wave propagation in solid media is reviewed. The advances and developments of theoretical investigations on the effect of SHG of ultrasonic guided wave propagation in different structures are addressed. Some obscure understandings and the ideas in dispute are also discussed.

Analysis of second-harmonic generation microscopy under refractive index mismatch

On the basis of the vector diffraction theory and Green＇s function method, this paper investigates the effects of refractive index mismatch on second-harmonic generation （SHG） microscopy. The polarization distribution and SHG intensity are calculated as functions of the sample radius and probe depth. The numerical results show that refractive index mismatch can result in peak intensity degradation, increase secondary lobes and extension of second- harmonic polarization distribution. Because of the attenuation of polarization intensity, the detected SHG intensity significantly decreases with increasing probe depth, which can limit the imaging depth of SHG microscopy inside thick samples. Forward SHG intensity decays slowly than backward SHG, due to the combination of extension second-harmonic polarization distribution and strong dependency of forward SHG on sample radius.

Optical second-harmonic generation of Janus MoSSe monolayer

The transition metal dichalcogenides(TMD)monolayers have shown strong second-harmonic generation(SHG)ow-ing to their lack of inversion symmetry.These ultrathin layers then serve as the frequency converters that can be intergraded on a chip.Here,taking MoSSe as an example,we report the first detailed experimental study of the SHG of Janus TMD monolayer,in which the transition metal layer is sandwiched by the two distinct chalcogen layers.It is shown that the SHG effectively arises from an in-plane second-harmonic polarization under paraxial focusing and detection.Based on this,the orientation-resolved SHG spectroscopy is realized to readily determine the zigzag and armchair axes of the Janus crystal with an accuracy better than±0.6°.Moreover,the SHG intensity is wavelength-dependent and can be greatly enhanced(~60 times)when the two-photon transition is resonant with the C-exciton state.Our findings uncover the SHG properties of Janus MoSSe monolayer,therefore lay the basis for its integrated frequency-doubling applications.

Phase Effects at Second-Harmonic Generation in ZnO/PMMA Nanocomposite Films

In this paper analysis of constant-intensity approximation of nonlinear interaction of the second-harmonic generation in ZnO/PMMA nanocomposite films for different concentrations of ZnO was carried out with regard to the losses and phase changes of all the interacting waves. The investigated samples were manufactured on the basis of ZnO nanoparticles embedded into polyvinylchloride polymeric matrix （PMMA） by the method of electrochemical deposition. The main goal of work is the exploration of the ZnO morphology and parameters of the second order susceptibilities. It is verified that the surface effects in ZnO/PMMA structures will give a larger contribution than the volume effects. The factors restricting the efficiency of the process of frequency conversion have been analyzed.

An optical tweezer-based microdroplet imaging technology

Microspheres can break the diffraction limit and magnify nano-structure imaging,and with its advantages of low cost and label-free operation,microsphere-assisted imaging has become an irreplaceable tool in the life sciences and for precision measurements.However,the tiny size and limited imaging field of traditional solid microspheres cause difficulties when imaging large sample areas.Alternatively,droplets have similar properties to those of microspheres,with large surface curvature and refractive-index difference from the surrounding environment,and they can also serve as lenses to focus light for observation and imaging.Previous work has shown that droplets with controllable size can be generated using an optical tweezer system and can be driven by optical traps to move precisely like solid microspheres.Here,a novel microdroplet-assisted imaging technology based on optical tweezers is proposed that better integrates the generation,manipulation,and utilization of droplets.

SAR image despeckling based on edge detection and nonsubsampled second generation bandelets

To preserve the sharp features and details of the synthetic aperture radar （SAR） image effectively when despeckling, a despeckling algorithm with edge detection in nonsubsampled second generation bandelet transform （NSBT） domain is proposed. First, the Canny operator is utilized to detect and remove edges from the SAR image. Then the NSBT which has an optimal approximation to the edges of images and a hard thresholding rule are used to approximate the details while despeckling the edge-removed image. Finally, the removed edges are added to the reconstructed image. As the edges axe detected and protected, and the NSBT is used, the proposed algorithm reaches the state-of-the-art effect which realizes both despeckling and preserving edges and details simultaneously. Experimental results show that both the subjective visual effect and the mainly objective performance indexes of the proposed algorithm outperform that of both Bayesian wavelet shrinkage with edge detection and Bayesian least square-Gaussian scale mixture （BLS-GSM）.