Reproduction of the Three-line Genic Male Sterile Line Parent Mian 7MB-1 （Brasscia Napus L.） and Seed Production of F1 Based on Somatic Tissue Culture

[Objective] The aim was to study the reproduction of the three-line genic male sterile （GMS） lineparent Mian7MB-1 （B. NapusL.） and the seed production of F1 through somatic tissue culture. [Methed] Through hybridization, a new breeding material Mian 7MB-1 in three-line genic temporary maintainer line propagated by tissue culture was used to improve the sterile plant rate of rapeseed in dual-purpose recessive GMS line, such as Mian 7AB type, S45AB type, and etc. And then the variety comparative test was performed. [Result] In order to avoid some fertility restoration phenomena occurring during the process of self-reproduction, Mian 7AB was propagated in bulk with somatic tissue culture of temporary maintainer line plant stem. The propagated temporary maintainer line seedlings were applied to the breeding and seed production of net room male sterile line parent, promoting the sterile plant rate of the male sterile line parent to 91.7% -93.5%. The male sterile line parents per hectare were enough for the seed production of hybrid F1 in 7 500 -15 000 hm^2. [ Conclusion ] Compared with the original dual-purpose GMS line, the seed production ultilizing male sterile line with high sterile plant rate greatly reduced the labor, significantly improved the seed yield, ensuring the seed quality and forming a perfect breeding and seed production system.

Key Techniques of Stereoscopic and Efficient Cultivation of Strawberry in Southern Xinjiang

Strawberry is rich in nutrition,which is the only fresh fruit in winter in southern Xinjiang,with good prospect of stereoscopic cultivation.In this paper,the key techniques such as stereoscopic cultivation pattern,the combination of different modes,the substrate of strawberry cultivation,the drip irrigation system,the formula of nutrient solution,the selection of varieties,the propagation of seed and seedling,the induction of flower buds,the planting and management of plants,and the green control of diseases and insect pests are introduced,and greenhouse space and solar energy are fully used to maximize the economic and social benefits of strawberry farming combined with tourism.The results will further promote the quality and efficiency of strawberry industry in southern Xinjiang,and provide a reference for strawberry growers.

Performance of elastic ball screen-cleaning device based on DEM-MBD coupling simulation

In seed breeding, the vibrating screen used for pre-sowing grading parental seeds often has the problem of poor self-purification rate. Using elastic balls to clean the plugging seeds and ensure the self-purification of the screen is a viable technical solution. To clarify and improve the operating performance of the elastic ball particles, a DEM-MBD coupling simulation model of the elastic ball screen-cleaning device was established in this study, and a numerical simulation analysis of the complex motion process was carried out. The mechanism of action of the elastic ball was explained more completely from the aspects of excitation force and energy transfer. Through the Plackett-Burman test, it was determined that the vibration frequency has the most significant effect on the excitation force. Multiple linear regression analysis was performed on each factor and the mathematical relationship equation. It was found that when the maximum excitation force was greater than 7 N and the average excitation force was greater than 2 N, the self-purification rate was greater than 95%. The self-purification rate was then increased to 99.81% by variable frequency tests. The variable frequency achieved better self-purification effect.