Plant seeds accumulate large amounts of protein and carbohydrate as storage reserves during matura- tion. Thus, understanding the genetic control of embryo and seed development may provide bioengi- neering tools for y...Plant seeds accumulate large amounts of protein and carbohydrate as storage reserves during matura- tion. Thus, understanding the genetic control of embryo and seed development may provide bioengi- neering tools for yield improvement. In this study, we report the identification of Retarded Embryo Development1 （RED1） gene in Arabidopsis, whose two independent T-DNA insertion mutant lines, SALK_085642 （red1-1 ） and SALK_022583 （red1-2）, show a retarded embryo development phenotype. The embryogenesis process ceases at the late heart stage in red1-1 and at the bent-cotyledon stage in red1-2, respectively, resulting in seed abortion in both lines. The retarded embryo development and seed abortion phenotypes reverted to normal when REDI complementation constructs were introduced into mutant plants. Small redl-2 homozygous plants can be successfully rescued by culturing immature seeds, indicating that seed abortion likely results from compromised tolerance to the desiccation process associated with seed maturation. Consistent with this observation, redl-2 seeds accumulate less protein, and the expression of two late embryo development reporter transgenes, LEA：：GUS and β-con- glycinin：：GUS, was significantly weak and started relatively late in the redl-2 mutant lines compared to the wild type. The REDI gene encodes a plant specific novel protein that is localized in the nucleus. These results indicate that RED1 plays important roles in embryo development, seed maturation and plant growth.展开更多
采用刀片破皮、低温冷冻、60℃热水浴及干燥、碱液处理、浓硫酸加搅拌等方法处理野生大豆种子,研究其对野生大豆种子发芽和胚生长的影响。结果表明:经过刀片破皮和浓硫酸加搅拌处理的各项萌发指标远高于对照(P<0.05);综合野生大豆幼...采用刀片破皮、低温冷冻、60℃热水浴及干燥、碱液处理、浓硫酸加搅拌等方法处理野生大豆种子,研究其对野生大豆种子发芽和胚生长的影响。结果表明:经过刀片破皮和浓硫酸加搅拌处理的各项萌发指标远高于对照(P<0.05);综合野生大豆幼苗的萌发指标和胚根长、胚芽及鲜重等指标,破皮和10 m in浓硫酸加搅拌处理最有利于野生大豆种子萌发;冷冻处理的各项萌发指标显著低于对照(P<0.05),与常温保存相比,冷藏保存野生大豆种子的千粒重和发芽率有所增加。表明冷藏保存可以提高野生大豆种子的质量;常温下野生大豆休眠期为7个月,冷藏保存野生大豆的休眠期为8个月。展开更多
基金supported by the UConn Office of the Vice President for Research,Research Excellence Program
文摘Plant seeds accumulate large amounts of protein and carbohydrate as storage reserves during matura- tion. Thus, understanding the genetic control of embryo and seed development may provide bioengi- neering tools for yield improvement. In this study, we report the identification of Retarded Embryo Development1 （RED1） gene in Arabidopsis, whose two independent T-DNA insertion mutant lines, SALK_085642 （red1-1 ） and SALK_022583 （red1-2）, show a retarded embryo development phenotype. The embryogenesis process ceases at the late heart stage in red1-1 and at the bent-cotyledon stage in red1-2, respectively, resulting in seed abortion in both lines. The retarded embryo development and seed abortion phenotypes reverted to normal when REDI complementation constructs were introduced into mutant plants. Small redl-2 homozygous plants can be successfully rescued by culturing immature seeds, indicating that seed abortion likely results from compromised tolerance to the desiccation process associated with seed maturation. Consistent with this observation, redl-2 seeds accumulate less protein, and the expression of two late embryo development reporter transgenes, LEA：：GUS and β-con- glycinin：：GUS, was significantly weak and started relatively late in the redl-2 mutant lines compared to the wild type. The REDI gene encodes a plant specific novel protein that is localized in the nucleus. These results indicate that RED1 plays important roles in embryo development, seed maturation and plant growth.
文摘采用刀片破皮、低温冷冻、60℃热水浴及干燥、碱液处理、浓硫酸加搅拌等方法处理野生大豆种子,研究其对野生大豆种子发芽和胚生长的影响。结果表明:经过刀片破皮和浓硫酸加搅拌处理的各项萌发指标远高于对照(P<0.05);综合野生大豆幼苗的萌发指标和胚根长、胚芽及鲜重等指标,破皮和10 m in浓硫酸加搅拌处理最有利于野生大豆种子萌发;冷冻处理的各项萌发指标显著低于对照(P<0.05),与常温保存相比,冷藏保存野生大豆种子的千粒重和发芽率有所增加。表明冷藏保存可以提高野生大豆种子的质量;常温下野生大豆休眠期为7个月,冷藏保存野生大豆的休眠期为8个月。
基金山西省农业科技成果转化和推广示范项目——强生态型缀花地被植物(绢毛匍匐委陵菜)示范与推广(SXNKTG 201801)山西省科技成果转化项目——绢毛匍匐委陵菜新品种示范及推广(201604 D 131054)山西省科技攻关项目——山西乡土芳香地被植物选优及扩繁体系构建(20140311013-4)
