In order to search the degradability of kraft lignin, the potential bacterial strains Bacillus subtilis（GU193980） and Klebsiella pneumoniae（GU193981） were isolated, screened and applied in axenic and co-culture co...In order to search the degradability of kraft lignin, the potential bacterial strains Bacillus subtilis（GU193980） and Klebsiella pneumoniae（GU193981） were isolated, screened and applied in axenic and co-culture conditions. Results revealed that mixed culture showed better decolorization efficiency（80%） and reduction of pollution parameters（COD 73% and BOD62%） than axenic culture. This indicated syntrophic growth of these two bacteria rather than any antagonistic effect. The HPLC analysis of degraded samples of kraft lignin has shown the reduction in peak area compared to control, suggesting that decrease in color intensity might be largely attributed to the degradation of lignin by isolated bacteria.Further, the GC-MS analysis showed that most of the compounds detected in control were diminished after bacterial treatment. Further, the seed germination test using Phaseolus aureus has supported the detoxification of bacterial decolorized kraft lignin for environmental safety. All these observations have revealed that the developed bacterial co-culture was capable for the effective degradation and decolorization of lignin containing rayon grade pulp mill wastewater for environmental safety.展开更多
基金financial assistance from Department of Science and Technology (DST) vide sanction order No. F. No SB/50/ BB-0042/2013 for project and UGC Kothari fellowship to Dr. Sangeeta Yadav is highly acknowledged
文摘In order to search the degradability of kraft lignin, the potential bacterial strains Bacillus subtilis（GU193980） and Klebsiella pneumoniae（GU193981） were isolated, screened and applied in axenic and co-culture conditions. Results revealed that mixed culture showed better decolorization efficiency（80%） and reduction of pollution parameters（COD 73% and BOD62%） than axenic culture. This indicated syntrophic growth of these two bacteria rather than any antagonistic effect. The HPLC analysis of degraded samples of kraft lignin has shown the reduction in peak area compared to control, suggesting that decrease in color intensity might be largely attributed to the degradation of lignin by isolated bacteria.Further, the GC-MS analysis showed that most of the compounds detected in control were diminished after bacterial treatment. Further, the seed germination test using Phaseolus aureus has supported the detoxification of bacterial decolorized kraft lignin for environmental safety. All these observations have revealed that the developed bacterial co-culture was capable for the effective degradation and decolorization of lignin containing rayon grade pulp mill wastewater for environmental safety.
