Quality of Irrigated Water with Nanometer Pottery Tray Treatment and Its Effects on Seed Soaking

To study the impacts of nanometer pottery trays （NPTs） on different rice varieties, three rice varieties, Zhongzheyou 1, Jinzao 47 and Xiushui 09, were treated with four NPTs （NPT-A, NPT-B, NPT-C and NPT-D） with different energies, respectively. The results showed that when the same rice variety was treated with different NPTs or different rice varieties were treated with the same NPT, the impacts on seed germination rate, seedling growth, plant height, panicle length and weight, the number of filled grains, seed-setting rate and 1000-grain weight varied between different rice variety-NPT interaction groups. In general, high energy NPT-C and NPT-D treatments obviously enhanced the functions of most examined rice characters. For example, NPT-C and NPT-D treatments improved the germination rate of all the three rice varieties, and promoted the root growth of seedlings, and increased seedling fresh weight, single panicle weight, filled grain number per panicle, seed-setting rate and 1000-grain weight. On the contrary, low energy NPT-A treatment restrained the seed germination rate in Jinzao 47 and Zhongzheyou 1, and decreased the seedling fresh weight in Zhongzheyou 1. NPT-B treatment restrained the seedling growth in Jinzao 47 but increased the panicle length of Zhongzheyou 1. NPT-A and NPT-C treatments obviously decreased the 1000-grain weight in Xiushui 09. Therefore, when treating crop seeds or plants using nanomaterials or nanotechnologies, different types of crops/varieties should select the nanomaterials or nanotechnologies with suitable energies to reduce the negative effects.

A comparison of the toxicity of landfill leachate exposure at the seed soaking and germination stages on Zea mays L.(maize)

To compare the toxicity of landfill leachate exposure at the early stages of seed soaking and germination on maize,a field experiment was conducted to evaluate the physiological aspects of growth,yield and potential clastogenicity of root-tip cells. The maizes were treated with leachate at levels of 2%,10%,20%,30% or 50%（V/V）. First,the change of physiological indexes,including chlorophyll（Chl）,Malondialdehyde（MDA）and Reactive oxygen species（ROS）levels,combined with yield all showed that soaking with leachate,but not germination,generated a greater ecological risk on maize. After a soaking treatment of maize with 50% leachate,the Chl,MDA and ROS levels during a vigorous growth period were 47.3%,149.8% and 309.7%,respectively,of the control,whereas the yield decreased to 68.6% of the control. In addition,our results demonstrated that the leachate at lower levels could promote growth. This is mainly embodied in that the yield of maize treated with 10%leachate at the soaking stage increased to 116.0% of the control. Moreover,the cytological analysis experiment also demonstrated that the ecological risk of leachate still exists in both cases. Furthermore,the gray relational analysis showed that the ear row number and tassel branch number were the major factors affecting the yield of maize treated with 50%leachate at the stages of soaking and germination,respectively. In general,these results are helpful in understanding the phytotoxicity of leachate,which provides additional reference data for risk assessment and management of leachate.

Effects of Acetylacetone Solution Soaking on Agrobacterium - transformed Maize Seed Buds

[Objectives]The effect of acetosyringone seed soaking on the transformation of maize seed buds was analyzed,so as to improve the genetic transformation efficiency of maize and to provide technical support for transgenic breeding of maize.[Methods]The seeds of the"Zheng 58"maize inbred line were used as experimental materials.When the seeds were germinated,AS was added to the water at concentrations of 70,140,210,and 280μmol/L,respectively,and the seeds germinated without the addition of AS served as the CK.The Agrobacterium-mediated method was used to transform bud growth points of maize seeds,and green fluorescent protein detection was performed on the young shoots transformed with EGFP(enhanced green fluorescent protein)gene.The effect of soaking seeds with acetosyringone solution on the transformation of maize bud growth points by Agrobacterium was studied according to the detection results.[Results]Soaking seeds in acetosyringone solutions for germination had the effect of inhibiting the germination of maize seeds and inhibiting sprout elongation,and the higher the concentration of acetosyringone,the stronger the inhibition.When the concentration of acetosyringone solution was 280μmol/L,the germination rate of seeds was only 36.2%of the CK,while soaking seeds with 70-140μmol/L acetosyringone solution for germination could not only ensure a higher germination rate of maize seeds,but also significantly increased the transformation efficiency of maize bud growth points.When the seeds were soaked with 70μmol/L acetosyringone solution for germination,the positive rate of transformed maize buds was the highest,reaching 32.1%.[Conclusions]When maize bud growth points were used as the receptor of Agrobacterium transformation,soaking seeds with 70-140μmol/L acetosyringone for germination basically did not affect the germination of seeds,and was beneficial to the activation of Agrobacterium,thereby promoting the transformation.

Effect of seed soaked with urea solution on the cold tolerance of rice seedlings

In the Yangtze river aera,the first crop of indi-ca rice is sown in late Mar to early Apr andtransplanted in early May.Usually,seedlingsperish when abnormal low temperature of 6-10℃ lasted for 3 d or longer.The effect ofsocking seed with urea solution on increasingthe cold tolerance at the seedling stage was

Control Efficiency of Different Treatments on Rice Bakanae Disease

After different treatments, germination of rice seeds and growth of young seedlings were observed, and the optimal seed soaking agent was selected by comparing seedling growth and incidence of bakanae disease in the fidd. The results showed that self-made powder （800 times） could promote indoor seed germina- tion and seedling growth, had no obvious inhibitory effect on the growth of seedlings in the field, and could reduce the incidence of bakanae disease of rice seed- lings, so it was the optimal treatment for controlling rice bakanae disease.