Calcium (Ca) plays an important role in the metabolism of higher plants. Recently, research on Ca^2+ in plants has been focused especially at the cellular and molecular levels. Uptake, transport, and distribution a...Calcium (Ca) plays an important role in the metabolism of higher plants. Recently, research on Ca^2+ in plants has been focused especially at the cellular and molecular levels. Uptake, transport, and distribution are also very important for Ca to accomplish its function at the whole-plant level. In this experiment, one-year-old apple seedlings (M. hupehensis Rehd.) were investigated to determine the distribution of stored Ca, the different forms of Ca, and Ca^2+-ATPase activity after treatment with indole butyric acid (IBA). The results showed that the total Ca measured in mature leaves and Ca^2+- ATPase activity in tender leaves were higher compared with those in the control (CK). Calcium nitrate and calcium chloride (ALe-Ca) and calcium phosphate and calcium carbonate (HAC-Ca) decreased in both mature leaves and shoots, whereas water-soluble calcium (H2O-Ca), calcium pectate (NaCl-Ca), and calcium oxalate (HCl-Ca) increased. The percentage of active calcium, calcium pectate, and water-soluble calcium increased, whereas the percentage of calcium phosphate and calcium carbonate decreased. When treated with IBA, calcium fractions and percentage of the different forms of Ca was enhanced in 40 part per million (ppm) IBA compared with 20 ppm IBA and water. The results indicated that IBA increased the percentage of both active calcium (NaCl-Ca and H2O-Ca) in tender shoots and boosted the transportation of stored Ca in plants. IBA promoted Ca^2+-ATPase activity and Ca^2+ uptake in tender shoots of M. hupehensis. It can improve the total Ca contents and the relative percentage of Ca.展开更多
This study investigated the effects of six crop rotation combinations on the soil quality of old apple orchard and seedling growth of Malus hupehensis Rehd.(apple rootstock) under pot conditions. The inhibitory effect...This study investigated the effects of six crop rotation combinations on the soil quality of old apple orchard and seedling growth of Malus hupehensis Rehd.(apple rootstock) under pot conditions. The inhibitory effects of crops such as Allium fistulosum, Brassica juncea, and Triticum aestivum on four species of Fusarium were observed and compared in six treatments. These were continuous cropping(CK), fumigation with the methyl bromide(FM), rotating A. fistulosum only(R1), rotating A. fistulosum and T. aestivum(R2), rotating A. fistulosum, B. juncea, and T. aestivum(R3), and fallow(FC) in a year. The results showed that the biomass of Malus hupehensis Rehd. seedlings increased significantly. The root length increased and the root architecture was optimized. The respiration rate of the root system was increased by about 1 time after rotation. The treatments of R1, R2, R3, and FC increased bacterial count by 232.17%, 96.04%, 316.21%, and 60.02%, respectively. However, the fungi were reduced in varying degrees and bacteria/fungi ratio was increased by 5–10 times. The enzyme activities, p H, and organic matter were increased, but soil bulk density was decreased. Phenolic acids such as phloridzin was decreased significantly. The copy number of four Fusarium species declined by 85.59%, 74.94%, 69.68%, and 54.41% after rotating three different crops(R3 treatment). The root volatiles of three plants inhibited mycelial growth and spore germination of four Fusarium species.展开更多
In order to provide more evidences for the mechanism of phasic change 4-year old (mature phase) apomictic seedlings of crabapple 〔Malus hupehensis (Panlp.) Rehd.〕 were selected as trial material, and 1-year-old seed...In order to provide more evidences for the mechanism of phasic change 4-year old (mature phase) apomictic seedlings of crabapple 〔Malus hupehensis (Panlp.) Rehd.〕 were selected as trial material, and 1-year-old seedling and 4-year-old grafted plant developed from the adult-phase bud were used as contrasts of juvenile zone and adult zone of perennial seedlings respectively. The height-associated characteristics of leaf physiological indexes were investigated. The result showed that, based on the fresh weight of leaves, the amounts per unit of total soluble sugar, reducing sugar, soluble protein, total nucleic acid and RNA increased with leaf position height rising in mature seedling, on the contrary, the content of DNA decreased. Their contents at different leaf positions above the lowest flowering point remained at the same levels. The content of non\|reducing sugar of leaves did not vary with different leaf positions in mature seedling and grafted plant. Total nucleic acid content per unit of fresh leaf weight increased along with the leaf position height of seedling, it is due to the increasing of RNA content. The amounts per unit of total soluble sugar, reducing sugar, soluble protein, total nucleic acid and RNA could serve as markers to assess characterization of phasic change.展开更多
基金The study was supported financially by the National Natural Science Foundation of China(30170655&30270923).
文摘Calcium (Ca) plays an important role in the metabolism of higher plants. Recently, research on Ca^2+ in plants has been focused especially at the cellular and molecular levels. Uptake, transport, and distribution are also very important for Ca to accomplish its function at the whole-plant level. In this experiment, one-year-old apple seedlings (M. hupehensis Rehd.) were investigated to determine the distribution of stored Ca, the different forms of Ca, and Ca^2+-ATPase activity after treatment with indole butyric acid (IBA). The results showed that the total Ca measured in mature leaves and Ca^2+- ATPase activity in tender leaves were higher compared with those in the control (CK). Calcium nitrate and calcium chloride (ALe-Ca) and calcium phosphate and calcium carbonate (HAC-Ca) decreased in both mature leaves and shoots, whereas water-soluble calcium (H2O-Ca), calcium pectate (NaCl-Ca), and calcium oxalate (HCl-Ca) increased. The percentage of active calcium, calcium pectate, and water-soluble calcium increased, whereas the percentage of calcium phosphate and calcium carbonate decreased. When treated with IBA, calcium fractions and percentage of the different forms of Ca was enhanced in 40 part per million (ppm) IBA compared with 20 ppm IBA and water. The results indicated that IBA increased the percentage of both active calcium (NaCl-Ca and H2O-Ca) in tender shoots and boosted the transportation of stored Ca in plants. IBA promoted Ca^2+-ATPase activity and Ca^2+ uptake in tender shoots of M. hupehensis. It can improve the total Ca contents and the relative percentage of Ca.
基金supported by the National Natural Science Foundation of China (Grant No. 31672104)China Agriculture Research System of MOF and MARA (Grant No. CARS-27)+4 种基金Shandong Agricultural Major Applied Technology Innovation Project (Grant No. SD2019ZZ008)Taishan Scholar Funded Project(Grant No. 20190923)Qingchuang Science and Technology Support Project of Shandong Colleges and Universities (Grant No.2019KJF020)Natural Science Foundation of Shandong Province(Grant No. ZR2020MC131)the National Key Research and Development Program of China (Grant No. 2020YFD1000201)。
文摘This study investigated the effects of six crop rotation combinations on the soil quality of old apple orchard and seedling growth of Malus hupehensis Rehd.(apple rootstock) under pot conditions. The inhibitory effects of crops such as Allium fistulosum, Brassica juncea, and Triticum aestivum on four species of Fusarium were observed and compared in six treatments. These were continuous cropping(CK), fumigation with the methyl bromide(FM), rotating A. fistulosum only(R1), rotating A. fistulosum and T. aestivum(R2), rotating A. fistulosum, B. juncea, and T. aestivum(R3), and fallow(FC) in a year. The results showed that the biomass of Malus hupehensis Rehd. seedlings increased significantly. The root length increased and the root architecture was optimized. The respiration rate of the root system was increased by about 1 time after rotation. The treatments of R1, R2, R3, and FC increased bacterial count by 232.17%, 96.04%, 316.21%, and 60.02%, respectively. However, the fungi were reduced in varying degrees and bacteria/fungi ratio was increased by 5–10 times. The enzyme activities, p H, and organic matter were increased, but soil bulk density was decreased. Phenolic acids such as phloridzin was decreased significantly. The copy number of four Fusarium species declined by 85.59%, 74.94%, 69.68%, and 54.41% after rotating three different crops(R3 treatment). The root volatiles of three plants inhibited mycelial growth and spore germination of four Fusarium species.
文摘In order to provide more evidences for the mechanism of phasic change 4-year old (mature phase) apomictic seedlings of crabapple 〔Malus hupehensis (Panlp.) Rehd.〕 were selected as trial material, and 1-year-old seedling and 4-year-old grafted plant developed from the adult-phase bud were used as contrasts of juvenile zone and adult zone of perennial seedlings respectively. The height-associated characteristics of leaf physiological indexes were investigated. The result showed that, based on the fresh weight of leaves, the amounts per unit of total soluble sugar, reducing sugar, soluble protein, total nucleic acid and RNA increased with leaf position height rising in mature seedling, on the contrary, the content of DNA decreased. Their contents at different leaf positions above the lowest flowering point remained at the same levels. The content of non\|reducing sugar of leaves did not vary with different leaf positions in mature seedling and grafted plant. Total nucleic acid content per unit of fresh leaf weight increased along with the leaf position height of seedling, it is due to the increasing of RNA content. The amounts per unit of total soluble sugar, reducing sugar, soluble protein, total nucleic acid and RNA could serve as markers to assess characterization of phasic change.