Nanoparticles can be used to purify proteins from plasma. We report here the purification of apolipoprotein A-I (apoA-I) with high specificity from human plasma using copolymeric nanoparticles. We present an optimized...Nanoparticles can be used to purify proteins from plasma. We report here the purification of apolipoprotein A-I (apoA-I) with high specificity from human plasma using copolymeric nanoparticles. We present an optimized protocol using 50:50 NiPAM:BAM copolymer nanoparticles with thermo-responsive properties as an affinity resin. Repeated pelleting and washing of nanoparticle-captured apoA-I is achieved through temperature cycling. The protein is then eluted using urea followed by an ion exchange step for protein concentration and depletion of nanoparticles.展开更多
The oxide sample NiO/CeO_2 with feed atomic ratio of Ni/Ce at 40%, prepared by co-precipitation method and calcination at 500 oC for 2 h, was impregnated by aqueous solution of NH_4Cl to dope chlorine ions. After the ...The oxide sample NiO/CeO_2 with feed atomic ratio of Ni/Ce at 40%, prepared by co-precipitation method and calcination at 500 oC for 2 h, was impregnated by aqueous solution of NH_4Cl to dope chlorine ions. After the impregnated samples were dried and calcined at 400 oC for 2 h, the calcined samples NiO（Cl_x）/CeO_2（x=0.1–0.5） were characterized by means of X-ray diffraction（XRD） and temperature programmed reduction（TPR） techniques. It was comfirmed that the doped chlorine ions hindered reduction of Ni^（2＋） ions in the calcined samples, and suppressed adsorption of CO_2 and CO on the reduced sample Ni（Cl_（0.3））/CeO_2. The reduced samples Ni（Cl_x）/CeO_2（x=0.0–0.5） were used as catalysts for selective methanation of CO in H_2-rich gas. When chlorine ions were doped at the feed atomic ratio of Cl/Ce（x） equal to 0.3–0.5, CO in the H_2-rich gas could be removed to below 10 ppm with a high selectivity more than 50% in a wide reaction temperature range of 220–280 oC. However, the selectivity of CO methanation decreased with reaction time in the durability tests over the catalyst Ni（Cl_（0.3））/CeO_2 at the reaction temperature of 260 oC and even at 220 oC. The lowering of the selectivity was found to be related with the surface composition change of the catalyst in the catalytic reaction.展开更多
基金This work was funded by an Irish Research Council for Science,Engineering and Technology Postdoctoral Fellowship(M.L.)the Marianne and Marcus Wallenberg Foundation(M.L.)+2 种基金the EU FP6 project NanoInteract(NMP4-CT-2006-033231)and the SFI SRC BioNanoInteract(07 SRC B1155)Centre for Nano-Vaccine,Copenhagen,Denmark,and the Swedish Research Council(VR).
文摘Nanoparticles can be used to purify proteins from plasma. We report here the purification of apolipoprotein A-I (apoA-I) with high specificity from human plasma using copolymeric nanoparticles. We present an optimized protocol using 50:50 NiPAM:BAM copolymer nanoparticles with thermo-responsive properties as an affinity resin. Repeated pelleting and washing of nanoparticle-captured apoA-I is achieved through temperature cycling. The protein is then eluted using urea followed by an ion exchange step for protein concentration and depletion of nanoparticles.
基金Project supported by the National Natural Science Foundation of China(21643008)
文摘The oxide sample NiO/CeO_2 with feed atomic ratio of Ni/Ce at 40%, prepared by co-precipitation method and calcination at 500 oC for 2 h, was impregnated by aqueous solution of NH_4Cl to dope chlorine ions. After the impregnated samples were dried and calcined at 400 oC for 2 h, the calcined samples NiO（Cl_x）/CeO_2（x=0.1–0.5） were characterized by means of X-ray diffraction（XRD） and temperature programmed reduction（TPR） techniques. It was comfirmed that the doped chlorine ions hindered reduction of Ni^（2＋） ions in the calcined samples, and suppressed adsorption of CO_2 and CO on the reduced sample Ni（Cl_（0.3））/CeO_2. The reduced samples Ni（Cl_x）/CeO_2（x=0.0–0.5） were used as catalysts for selective methanation of CO in H_2-rich gas. When chlorine ions were doped at the feed atomic ratio of Cl/Ce（x） equal to 0.3–0.5, CO in the H_2-rich gas could be removed to below 10 ppm with a high selectivity more than 50% in a wide reaction temperature range of 220–280 oC. However, the selectivity of CO methanation decreased with reaction time in the durability tests over the catalyst Ni（Cl_（0.3））/CeO_2 at the reaction temperature of 260 oC and even at 220 oC. The lowering of the selectivity was found to be related with the surface composition change of the catalyst in the catalytic reaction.
