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Defect Detection Model Using Time Series Data Augmentation and Transformation
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作者 Gyu-Il Kim Hyun Yoo +1 位作者 Han-Jin Cho Kyungyong Chung 《Computers, Materials & Continua》 SCIE EI 2024年第2期1713-1730,共18页
Time-series data provide important information in many fields,and their processing and analysis have been the focus of much research.However,detecting anomalies is very difficult due to data imbalance,temporal depende... Time-series data provide important information in many fields,and their processing and analysis have been the focus of much research.However,detecting anomalies is very difficult due to data imbalance,temporal dependence,and noise.Therefore,methodologies for data augmentation and conversion of time series data into images for analysis have been studied.This paper proposes a fault detection model that uses time series data augmentation and transformation to address the problems of data imbalance,temporal dependence,and robustness to noise.The method of data augmentation is set as the addition of noise.It involves adding Gaussian noise,with the noise level set to 0.002,to maximize the generalization performance of the model.In addition,we use the Markov Transition Field(MTF)method to effectively visualize the dynamic transitions of the data while converting the time series data into images.It enables the identification of patterns in time series data and assists in capturing the sequential dependencies of the data.For anomaly detection,the PatchCore model is applied to show excellent performance,and the detected anomaly areas are represented as heat maps.It allows for the detection of anomalies,and by applying an anomaly map to the original image,it is possible to capture the areas where anomalies occur.The performance evaluation shows that both F1-score and Accuracy are high when time series data is converted to images.Additionally,when processed as images rather than as time series data,there was a significant reduction in both the size of the data and the training time.The proposed method can provide an important springboard for research in the field of anomaly detection using time series data.Besides,it helps solve problems such as analyzing complex patterns in data lightweight. 展开更多
关键词 Defect detection time series deep learning data augmentation data transformation
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A Time Series Intrusion Detection Method Based on SSAE,TCN and Bi-LSTM
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作者 Zhenxiang He Xunxi Wang Chunwei Li 《Computers, Materials & Continua》 SCIE EI 2024年第1期845-871,共27页
In the fast-evolving landscape of digital networks,the incidence of network intrusions has escalated alarmingly.Simultaneously,the crucial role of time series data in intrusion detection remains largely underappreciat... In the fast-evolving landscape of digital networks,the incidence of network intrusions has escalated alarmingly.Simultaneously,the crucial role of time series data in intrusion detection remains largely underappreciated,with most systems failing to capture the time-bound nuances of network traffic.This leads to compromised detection accuracy and overlooked temporal patterns.Addressing this gap,we introduce a novel SSAE-TCN-BiLSTM(STL)model that integrates time series analysis,significantly enhancing detection capabilities.Our approach reduces feature dimensionalitywith a Stacked Sparse Autoencoder(SSAE)and extracts temporally relevant features through a Temporal Convolutional Network(TCN)and Bidirectional Long Short-term Memory Network(Bi-LSTM).By meticulously adjusting time steps,we underscore the significance of temporal data in bolstering detection accuracy.On the UNSW-NB15 dataset,ourmodel achieved an F1-score of 99.49%,Accuracy of 99.43%,Precision of 99.38%,Recall of 99.60%,and an inference time of 4.24 s.For the CICDS2017 dataset,we recorded an F1-score of 99.53%,Accuracy of 99.62%,Precision of 99.27%,Recall of 99.79%,and an inference time of 5.72 s.These findings not only confirm the STL model’s superior performance but also its operational efficiency,underpinning its significance in real-world cybersecurity scenarios where rapid response is paramount.Our contribution represents a significant advance in cybersecurity,proposing a model that excels in accuracy and adaptability to the dynamic nature of network traffic,setting a new benchmark for intrusion detection systems. 展开更多
关键词 Network intrusion detection bidirectional long short-term memory network time series stacked sparse autoencoder temporal convolutional network time steps
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Preliminary research and scheme design of deep underground in situ geo-information detection experiment for Geology in Time
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作者 Heping Xie Ru Zhang +13 位作者 Zetian Zhang Yinshuang Ai Jianhui Deng Yun Chen Yong Zhou Mingchuan Li Liqiang Liu Mingzhong Gao Zeqian Yang Weiqiang Ling Heng Gao Qijun Hao Kun Xiao Chendi Lou 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2024年第1期1-13,共13页
The deep earth,deep sea,and deep space are the main parts of the national“three deep”strategy,which is in the forefront of the strategic deployment clearly defined in China’s 14th Five-Year Plan(2021-2025)and the L... The deep earth,deep sea,and deep space are the main parts of the national“three deep”strategy,which is in the forefront of the strategic deployment clearly defined in China’s 14th Five-Year Plan(2021-2025)and the Long-Range Objectives Through the Year 2035.It is important to reveal the evolutionary process and mechanism of deep tectonics to understand the earth’s past,present and future.The academic con-notation of Geology in Time has been given for the first time,which refers to the multi-field evolution response process of geological bodies at different time and spatial scales caused by geological processes inside and outside the Earth.Based on the deep in situ detection space and the unique geological envi-ronment of China Jinping Underground Laboratory,the scientific issue of the correlation mechanism and law between deep internal time-varying and shallow geological response is given attention.Innovative research and frontier exploration on deep underground in situ geo-information detection experiments for Geology in Time are designed to be carried out,which will have the potential to explore the driving force of Geology in Time,reveal essential laws of deep earth science,and explore innovative technologies in deep underground engineering. 展开更多
关键词 Deep underground Geology in time China Jinping Underground Laboratory In situ detection
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Cross-Dimension Attentive Feature Fusion Network for Unsupervised Time-Series Anomaly Detection
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作者 Rui Wang Yao Zhou +2 位作者 Guangchun Luo Peng Chen Dezhong Peng 《Computer Modeling in Engineering & Sciences》 SCIE EI 2024年第6期3011-3027,共17页
Time series anomaly detection is crucial in various industrial applications to identify unusual behaviors within the time series data.Due to the challenges associated with annotating anomaly events,time series reconst... Time series anomaly detection is crucial in various industrial applications to identify unusual behaviors within the time series data.Due to the challenges associated with annotating anomaly events,time series reconstruction has become a prevalent approach for unsupervised anomaly detection.However,effectively learning representations and achieving accurate detection results remain challenging due to the intricate temporal patterns and dependencies in real-world time series.In this paper,we propose a cross-dimension attentive feature fusion network for time series anomaly detection,referred to as CAFFN.Specifically,a series and feature mixing block is introduced to learn representations in 1D space.Additionally,a fast Fourier transform is employed to convert the time series into 2D space,providing the capability for 2D feature extraction.Finally,a cross-dimension attentive feature fusion mechanism is designed that adaptively integrates features across different dimensions for anomaly detection.Experimental results on real-world time series datasets demonstrate that CAFFN performs better than other competing methods in time series anomaly detection. 展开更多
关键词 time series anomaly detection unsupervised feature learning feature fusion
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Effects of Standing Time during Pretreatment on the Nitrite Concentration Detected by Spectrophotometric Method
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作者 Yingfei Zeng Juan Hu +2 位作者 Xianglong Bian Qianfeng Xia Tingwei Hu 《Journal of Materials Science and Chemical Engineering》 2024年第2期73-83,共11页
Food safety problems caused by excessive nitrite addition have been frequently reported and the detection of nitrite in food is particularly important. The standing time during the pretreatment of primary sample has a... Food safety problems caused by excessive nitrite addition have been frequently reported and the detection of nitrite in food is particularly important. The standing time during the pretreatment of primary sample has a great influence on the concentration of nitrite tested by spectrophotometric method. In this context, three kinds of food samples are prepared, including canned mustard, canned fish and home-made pickled water. A series of standing times are placed during the sample pretreatments and the corresponding nitrite contents in these samples are detected by spectrophotometric method based on N-ethylenediamine dihydrochloride. This study aims to find out a reasonable standing time during the pretreatment of food sample, providing influence factor for precise detection of nitrite. 展开更多
关键词 Standing time Spectrophotometric Method Nitrite detection
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A CNN-Based Single-Stage Occlusion Real-Time Target Detection Method
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作者 Liang Liu Nan Yang +4 位作者 Saifei Liu Yuanyuan Cao Shuowen Tian Tiancheng Liu Xun Zhao 《Journal of Intelligent Learning Systems and Applications》 2024年第1期1-11,共11页
Aiming at the problem of low accuracy of traditional target detection methods for target detection in endoscopes in substation environments, a CNN-based real-time detection method for masked targets is proposed. The m... Aiming at the problem of low accuracy of traditional target detection methods for target detection in endoscopes in substation environments, a CNN-based real-time detection method for masked targets is proposed. The method adopts the overall design of backbone network, detection network and algorithmic parameter optimisation method, completes the model training on the self-constructed occlusion target dataset, and adopts the multi-scale perception method for target detection. The HNM algorithm is used to screen positive and negative samples during the training process, and the NMS algorithm is used to post-process the prediction results during the detection process to improve the detection efficiency. After experimental validation, the obtained model has the multi-class average predicted value (mAP) of the dataset. It has general advantages over traditional target detection methods. The detection time of a single target on FDDB dataset is 39 ms, which can meet the need of real-time target detection. In addition, the project team has successfully deployed the method into substations and put it into use in many places in Beijing, which is important for achieving the anomaly of occlusion target detection. 展开更多
关键词 Real-time Mask Target CNN (Convolutional Neural Network) Single-Stage detection Multi-Scale Feature Perception
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A new method for coseismic offset detection from GPS coordinate time series
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作者 Zhiwei Yang Guangyu Xu +3 位作者 Tengxu Zhang Mingkai Chen FeiWu Zhiping Chen 《Geodesy and Geodynamics》 EI CSCD 2023年第6期551-558,共8页
Currently,the extraction of coseismic offset signals primarily relies on earthquake catalog data to determine the occurrence time of earthquakes.This is followed by the process of differencing the average GPS coordina... Currently,the extraction of coseismic offset signals primarily relies on earthquake catalog data to determine the occurrence time of earthquakes.This is followed by the process of differencing the average GPS coordinate time series data,with a time interval of 3 to 5 days before and after the earthquake.In the face of the huge amount of GPS coordinate time series data today,the conventional approach of relying on earthquake catalog data to assist in obtaining coseismic offset signals has become increasingly burdensome.To address this problem,we propose a new method for automatically detecting coseismic offset signals in GPS coordinate time series without an extra earthquake catalog for reference.Firstly,we pre-process the GPS coordinate time series data for filtering out stations with significant observations missing and detecting and removing outliers.Secondly,we eliminate other signals and errors in the GPS coordinate time series,such as trend and seasonal signals,leaving the coseismic offset signals as the primary signal.The resulting coordinate time series is then modeled using the first-order difference and data stacking method.The modeling method enables automatic detection of the coseismic offset signals in the GPS coordinate time series.The aforementioned method is applied to automatically detect coseismic offset signals using simulated data and the Searles Valley GPS data in California,USA.The results demonstrate the efficacy of our proposed method,successfully detecting coseismic offsets from vast amounts of GPS coordinate time series data. 展开更多
关键词 GPS Coordinate time series Coseismic offset Signal detection
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A Memory-Guided Anomaly Detection Model with Contrastive Learning for Multivariate Time Series
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作者 Wei Zhang Ping He +2 位作者 Ting Li Fan Yang Ying Liu 《Computers, Materials & Continua》 SCIE EI 2023年第11期1893-1910,共18页
Some reconstruction-based anomaly detection models in multivariate time series have brought impressive performance advancements but suffer from weak generalization ability and a lack of anomaly identification.These li... Some reconstruction-based anomaly detection models in multivariate time series have brought impressive performance advancements but suffer from weak generalization ability and a lack of anomaly identification.These limitations can result in the misjudgment of models,leading to a degradation in overall detection performance.This paper proposes a novel transformer-like anomaly detection model adopting a contrastive learning module and a memory block(CLME)to overcome the above limitations.The contrastive learning module tailored for time series data can learn the contextual relationships to generate temporal fine-grained representations.The memory block can record normal patterns of these representations through the utilization of attention-based addressing and reintegration mechanisms.These two modules together effectively alleviate the problem of generalization.Furthermore,this paper introduces a fusion anomaly detection strategy that comprehensively takes into account the residual and feature spaces.Such a strategy can enlarge the discrepancies between normal and abnormal data,which is more conducive to anomaly identification.The proposed CLME model not only efficiently enhances the generalization performance but also improves the ability of anomaly detection.To validate the efficacy of the proposed approach,extensive experiments are conducted on well-established benchmark datasets,including SWaT,PSM,WADI,and MSL.The results demonstrate outstanding performance,with F1 scores of 90.58%,94.83%,91.58%,and 91.75%,respectively.These findings affirm the superiority of the CLME model over existing stateof-the-art anomaly detection methodologies in terms of its ability to detect anomalies within complex datasets accurately. 展开更多
关键词 Anomaly detection multivariate time series contrastive learning memory network
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Fine-Grained Multivariate Time Series Anomaly Detection in IoT
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作者 Shiming He Meng Guo +4 位作者 Bo Yang Osama Alfarraj Amr Tolba Pradip Kumar Sharma Xi’ai Yan 《Computers, Materials & Continua》 SCIE EI 2023年第6期5027-5047,共21页
Sensors produce a large amount of multivariate time series data to record the states of Internet of Things(IoT)systems.Multivariate time series timestamp anomaly detection(TSAD)can identify timestamps of attacks and m... Sensors produce a large amount of multivariate time series data to record the states of Internet of Things(IoT)systems.Multivariate time series timestamp anomaly detection(TSAD)can identify timestamps of attacks and malfunctions.However,it is necessary to determine which sensor or indicator is abnormal to facilitate a more detailed diagnosis,a process referred to as fine-grained anomaly detection(FGAD).Although further FGAD can be extended based on TSAD methods,existing works do not provide a quantitative evaluation,and the performance is unknown.Therefore,to tackle the FGAD problem,this paper first verifies that the TSAD methods achieve low performance when applied to the FGAD task directly because of the excessive fusion of features and the ignoring of the relationship’s dynamic changes between indicators.Accordingly,this paper proposes a mul-tivariate time series fine-grained anomaly detection(MFGAD)framework.To avoid excessive fusion of features,MFGAD constructs two sub-models to independently identify the abnormal timestamp and abnormal indicator instead of a single model and then combines the two kinds of abnormal results to detect the fine-grained anomaly.Based on this framework,an algorithm based on Graph Attention Neural Network(GAT)and Attention Convolutional Long-Short Term Memory(A-ConvLSTM)is proposed,in which GAT learns temporal features of multiple indicators to detect abnormal timestamps and A-ConvLSTM captures the dynamic relationship between indicators to identify abnormal indicators.Extensive simulations on a real-world dataset demonstrate that the proposed algorithm can achieve a higher F1 score and hit rate than the extension of existing TSAD methods with the benefit of two independent sub-models for timestamp and indicator detection. 展开更多
关键词 Multivariate time series graph attention neural network fine-grained anomaly detection
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Aviation multi-station collaborative detecting based on time-frequency correlation of data-link 被引量:1
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作者 Bo Wang Xiaolong Liang +1 位作者 Liang Wei Pingni Liu 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2017年第5期827-840,共14页
As an important application research topic of the intelligent aviation multi-station, collaborative detecting must overcome the problem of scouting measurement with status of 'fragmentation', and the NP-hardne... As an important application research topic of the intelligent aviation multi-station, collaborative detecting must overcome the problem of scouting measurement with status of 'fragmentation', and the NP-hardness problem of matching association between target and measurement in the process of scouting to data-link, which has complicated technical architecture of network construction. In this paper, taking advantage of cooperation mechanism on signal level in the aviation multi-station sympathetic network, a method of obtaining target time difference of arrival (TDOA) measurement using multi-station collaborative detecting based on time-frequency association is proposed. The method can not only achieve matching between target and its measurement, but also obtain TDOA measurement by further evolutionary transaction through refreshing sequential pulse time of arrival (TOA) measurement matrix for matching and correlating. Simulation results show that the accuracy of TDOA measurement has significant superiority over TOA, and detection probability of false TDOA measurement introduced by noise and fake measurement can be reduced effectively. 展开更多
关键词 data-link time-frequency correlation aviation multistation synergistic detection time difference of arrival (TDOA)
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基于Real-time PCR法检测乳粉中牛源性成分定量研究
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作者 陈晨 史国华 +5 位作者 陈勃旭 张瑞 王玉欣 贾文珅 陈佳 周巍 《粮油食品科技》 CAS CSCD 北大核心 2024年第2期159-164,共6页
基于Real-timePCR建立了乳粉中牛源性成分相对定量检测方法,并对牛的特异性引物与探针进行了特异性、灵敏度和稳定性测试。通过模拟不同浓度牛乳粉与马乳粉混合样本,根据其△Ct值的函数关系进行线性拟合进而绘制标准曲线,建立乳粉中牛... 基于Real-timePCR建立了乳粉中牛源性成分相对定量检测方法,并对牛的特异性引物与探针进行了特异性、灵敏度和稳定性测试。通过模拟不同浓度牛乳粉与马乳粉混合样本,根据其△Ct值的函数关系进行线性拟合进而绘制标准曲线,建立乳粉中牛源性成分的相对定量检测。结果显示,该方法的最低检测限为0.00001 mg/mL,回收率为91.11%~119.2%,组间变异系数≤0.58%、组内变异系数≤1.44%。说明该方法在特异性与稳定性上适用于乳粉中牛源性成分及含量的掺假检测。 展开更多
关键词 牛乳粉 马乳粉 Real-time PCR 掺假检测
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一种基于real-time PCR技术的TTV检测方法的建立及应用
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作者 贾毅博 王高玉 +4 位作者 邓宛心 林彩云 杨华 陈运春 尹飞飞 《海南医学院学报》 CAS 北大核心 2024年第7期489-497,共9页
目的:本研究旨在开发一种具有更高灵敏度和特异性的TTV检测技术,为揭示TTV在多种疾病过程中的作用提供重要的技术支持。方法:为了更精确、灵敏的检测TTV,本研究分析了目前公布的所有亚型的TTV基因序列,在此基础上建立了一种基于UTR区域... 目的:本研究旨在开发一种具有更高灵敏度和特异性的TTV检测技术,为揭示TTV在多种疾病过程中的作用提供重要的技术支持。方法:为了更精确、灵敏的检测TTV,本研究分析了目前公布的所有亚型的TTV基因序列,在此基础上建立了一种基于UTR区域的real-time PCR检测方法,并与文献报道应用较为广泛的PCR检测方法进行了对比。结果:本研究建立的方法在1×10^(7)~1×10^(1) copies/μL标准品浓度范围内具有良好的线性关系,相关系数为1.000,斜率为-3.446,检测下限为1×10^(1) copies/μL。重复性试验结果显示,组内变异系数为7.22%,表明本方法重复性、稳定性较强。针对30份临床样本,使用本研究建立的real-time PCR检测方法及目前被多个研究所使用的4套引物进行对比。结果表明,本研究所建立的方法灵敏度显著高于文献中报道的4种方法(P<0.01);Sanger测序结果表明,本方法检测出的30份阳性样本均为TTV,检测特异性为100%。结论:本研究采用基于TaqMan探针的real-time PCR检测方法,检测灵敏性高、覆盖基因型范围广,尤其对于TTV病毒载量较低的情况下能够进行定量检测,对于TTV病毒的致病性及作为免疫标志物的应用提供重要的技术支持。 展开更多
关键词 Torque teno virus 基因组扩增测序 Real-time PCR检测
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Detecting damage to offshore platform structures using the time-domain data 被引量:1
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作者 程远胜 王真 《Journal of Marine Science and Application》 2008年第1期7-14,共8页
A new method that uses time-domain response data under random loading is proposed for detecting damage to the structural elements of offshore platforms. In our study, a time series model with a fitting order was first... A new method that uses time-domain response data under random loading is proposed for detecting damage to the structural elements of offshore platforms. In our study, a time series model with a fitting order was first constructed using the time-domain of noise data. A sensitivity matrix consisting of the first differential of the autoregressive coefficients of the time series models with respect to the stiffness of structural elements was then obtained based on time-domain response data. Locations and severity of damage may then be estimated by solving the damage vector whose components express the degrees of damage to the structural elements. A unique aspect of this detection method is that it requires acceleration history data from only one or a few sensors. This makes it feasible for a limited array of sensors to obtain sufficient data. The efficiency and reliability of the proposed method was demonstrated by applying it to a simplified offshore platform with damage to one element. Numerical simulations show that the use of a few sensors’ acceleration history data, when compared with recorded levels of noise, is capable of detecting damage efficiently. An increase in the number of sensors helps improve the diagnosis success rate. 展开更多
关键词 近海平台 损伤检测 检测技术 建筑物
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Real-Time Method for Detecting Harmonic and Reactive Currents of Single-Phase Circuits 被引量:1
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作者 张秀峰 丁菊霞 《Journal of Southwest Jiaotong University(English Edition)》 2006年第2期135-141,共7页
According to the characteristics of single-phase circuits and demand of using active filter for real-time detecting harmonic and reactive currents, a detecting method based on Fryze's power definition is proposed. Th... According to the characteristics of single-phase circuits and demand of using active filter for real-time detecting harmonic and reactive currents, a detecting method based on Fryze's power definition is proposed. The results of theoretical analysis and simula- tion show that the proposed method is effective in realtime detecting of instantaneous harmonic and reactive currents in single-phase circuits. When only detecting the total reactive currents, this method does not need a phase-locked loop circuit, and it also can be used in some special applications to provide different compensations on the ground of different requirements of electric network. Compared with the other methods based on the theory of instantaneous reactive power, this method is simple and easy to realize. 展开更多
关键词 Active filter HARMONIC Reactive current Real-time detection Single-phase circuit Electric-network
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GAS DETECTING AND FORECASTING VIA TIME SERIES METHOD
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作者 黄养光 《Journal of China University of Mining and Technology》 1990年第1期83-92,共10页
The importance and urgency of gas detecting and forecasting in underground coal mining are self-evident. Unfortunately, this problem has not yet been solved thoroughly. In this paper, the author suggests that the time... The importance and urgency of gas detecting and forecasting in underground coal mining are self-evident. Unfortunately, this problem has not yet been solved thoroughly. In this paper, the author suggests that the time series analysis method be adopted for processing the gas stochastic data. The time series method is superior to the conventional Fourier analysis in some aspects, especially, the time series method possesses Forecasting (or prediction) function which is highly valuable for gas monitoring. An example ot a set ot gas data sampled From a certain foul coal mine is investigated and an AR (3) model is established. The fitting result and the forecasting error are accepted satisfactorily. At the end of this paper several remarks are presented for further discussion. 展开更多
关键词 地下煤矿 瓦斯 预测模型 时序模型分析 涌出规律
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Synchronously Detecting Allergenic Ingredients of Peanut and Sesame in Food by Real-time Fluorescent PCR
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作者 Yongxin WANG Xiao CHENG +3 位作者 Yeju LU Hong AN Bo ZHANG Juanjuan LIU 《Agricultural Biotechnology》 CAS 2014年第3期1-3,共3页
Peanut,sesame and other raw materials of food are allergens for special populations.In this study,specific primers and TaqMan probes labeled by different fluorescences were designed targeting Ara h 2 gene of peanut an... Peanut,sesame and other raw materials of food are allergens for special populations.In this study,specific primers and TaqMan probes labeled by different fluorescences were designed targeting Ara h 2 gene of peanut and Ses i 1 gene of sesame.After the optimization of reaction conditions,a real-time fluorescent PCR method was established for simultaneous detection of allergenic ingredients of peanut and sesame in food.Genomic DNA samples of peanut,sesame,rice,wheat,barley,soybean,celery,maize,potato,tomato,walnut,groundnut in shell,cashew nut,sunflower seed,almond,apple,pear and strawberry,pork,beef,mutton and fish were used as templates for PCR amplification with deionized water as negative control template.Results indicated that the established real-time fluorescent PCR method could specifically identify allergenic ingredients of peanut and sesame simultaneously.Sensitivity test showed that the minimum detection limit of this method was 0.01%.Therefore,the established real-time fluorescent PCR method is a specific,sensitive and effective assay for simultaneously detecting allergenic ingredients of peanut and sesame in food. 展开更多
关键词 Real-time fluorescent PCR PEANUT SESAME Allergen detection
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Development and Preliminary Application of SYBR Green I Real-Time Fluorescence Quantitative PCR Method for Detecting Porcine Parvovirus Virus
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作者 SHEN Zhi-qiang WANG Jin-liang +3 位作者 GUO Xian-po WANG Xiao-hu WANG Ming ZHAO De-ming 《Animal Husbandry and Feed Science》 CAS 2009年第11期42-46,共5页
According to VP2 gene sequence of the porcine parvovirus virus strain NADL-2 (NC001718) available in GenBank (NC_001718), a pair of specific primer was designed, and the target fragment of 431 bp was obtained by P... According to VP2 gene sequence of the porcine parvovirus virus strain NADL-2 (NC001718) available in GenBank (NC_001718), a pair of specific primer was designed, and the target fragment of 431 bp was obtained by PCR amplification. The products were ligated with pMD18- T vector and then transformed into bacteria DH5α for recombinant plasmid extraction. After PCR identification and sequencing, recombinant plasmid was used as a standard template to establish the standard curve of SYBR Green I fluorescence quantitative PCR. Sensitivity test, specificity test and repeatability test were also determined. The results indicated that there was a good linear relationship between threshold cycle of the standard curve and template concentration, R2 =0.997 6. Tm ranged from 82.3 to 82.9 ℃, while the sensitivity was 72.1 copies/μl with good specificity and repeatability. The developed SYBR Green I real-time quantitative PCR method to detect PPV VP2 gene laid the basis for further studies on patho- oenesis, early clinical diaonosis of this virus and quantitative analysis of PPV infection. 展开更多
关键词 Porcine parvovirus virus Real-time fluorescence quantitative PCR detectION
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Cyber Resilience through Real-Time Threat Analysis in Information Security
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作者 Aparna Gadhi Ragha Madhavi Gondu +1 位作者 Hitendra Chaudhary Olatunde Abiona 《International Journal of Communications, Network and System Sciences》 2024年第4期51-67,共17页
This paper examines how cybersecurity is developing and how it relates to more conventional information security. Although information security and cyber security are sometimes used synonymously, this study contends t... This paper examines how cybersecurity is developing and how it relates to more conventional information security. Although information security and cyber security are sometimes used synonymously, this study contends that they are not the same. The concept of cyber security is explored, which goes beyond protecting information resources to include a wider variety of assets, including people [1]. Protecting information assets is the main goal of traditional information security, with consideration to the human element and how people fit into the security process. On the other hand, cyber security adds a new level of complexity, as people might unintentionally contribute to or become targets of cyberattacks. This aspect presents moral questions since it is becoming more widely accepted that society has a duty to protect weaker members of society, including children [1]. The study emphasizes how important cyber security is on a larger scale, with many countries creating plans and laws to counteract cyberattacks. Nevertheless, a lot of these sources frequently neglect to define the differences or the relationship between information security and cyber security [1]. The paper focus on differentiating between cybersecurity and information security on a larger scale. The study also highlights other areas of cybersecurity which includes defending people, social norms, and vital infrastructure from threats that arise from online in addition to information and technology protection. It contends that ethical issues and the human factor are becoming more and more important in protecting assets in the digital age, and that cyber security is a paradigm shift in this regard [1]. 展开更多
关键词 Cybersecurity Information Security Network Security Cyber Resilience Real-time Threat Analysis Cyber Threats Cyberattacks Threat Intelligence Machine Learning Artificial Intelligence Threat detection Threat Mitigation Risk Assessment Vulnerability Management Incident Response Security Orchestration Automation Threat Landscape Cyber-Physical Systems Critical Infrastructure Data Protection Privacy Compliance Regulations Policy Ethics CYBERCRIME Threat Actors Threat Modeling Security Architecture
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Output only modal identification and structural damage detection using time frequency & wavelet techniques 被引量:13
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作者 S.Nagarajaiah B.Basu 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2009年第4期583-605,共23页
The primary objective of this paper is to develop output only modal identification and structural damage detection. Identification of multi-degree of freedom (MDOF) linear time invariant (LTI) and linear time vari... The primary objective of this paper is to develop output only modal identification and structural damage detection. Identification of multi-degree of freedom (MDOF) linear time invariant (LTI) and linear time variant (LTV--due to damage) systems based on Time-frequency (TF) techniques--such as short-time Fourier transform (STFT), empirical mode decomposition (EMD), and wavelets--is proposed. STFT, EMD, and wavelet methods developed to date are reviewed in detail. In addition a Hilbert transform (HT) approach to determine frequency and damping is also presented. In this paper, STFT, EMD, HT and wavelet techniques are developed for decomposition of free vibration response of MDOF systems into their modal components. Once the modal components are obtained, each one is processed using Hilbert transform to obtain the modal frequency and damping ratios. In addition, the ratio of modal components at different degrees of freedom facilitate determination of mode shape. In cases with output only modal identification using ambient/random response, the random decrement technique is used to obtain free vibration response. The advantage of TF techniques is that they arc signal based; hence, can be used for output only modal identification. A three degree of freedom 1:10 scale model test structure is used to validate the proposed output only modal identification techniques based on STFT, EMD, HT, wavelets. Both measured free vibration and forced vibration (white noise) response are considered. The secondary objective of this paper is to show the relative ease with which the TF techniques can be used for modal identification and their potential for real world applications where output only identification is essential. Recorded ambient vibration data processed using techniques such as the random decrement technique can be used to obtain the free vibration response, so that further processing using TF based modal identification can be performed. 展开更多
关键词 time-frequency methods short time Fourier transform Hilbert transform WAVELETS modal identification:output only structural health monitoring damage detection
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Statistical moment-based structural damage detection method in time domain 被引量:7
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作者 J.Zhang Y.L.Xu +2 位作者 J.Li Y.Xia J.C.Li 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2013年第1期13-23,共11页
A novel structural damage detection method with a new damage index,i.e.,the statistical moment-based damage detection(SMBDD) method in the frequency domain,has been recently proposed.The aim of this study is to exte... A novel structural damage detection method with a new damage index,i.e.,the statistical moment-based damage detection(SMBDD) method in the frequency domain,has been recently proposed.The aim of this study is to extend the SMBDD method in the frequency domain to the time domain for building structures subjected to non-Gaussian and non-stationary excitations.The applicability and effectiveness of the SMBDD method in the time domainis verified both numerically and experimentally.Shear buildings with various damage scenarios are first numerically investigated in the time domain taking into account the effect of measurement noise.The applicability of the proposed method in the time domain to building structures subjected to non-Gaussian and non-stationary excitations is then experimentally investigated through a series of shaking table tests,in which two three-story shear building models with four damage scenarios aretested.The identified damage locations and severities are then compared with the preset values.The comparative results are found to be satisfactory,and the SMBDD method is shown to be feasible and effective for building structures subjected to non-Gaussian and non-stationary excitations. 展开更多
关键词 damage detection statistical moment time domain NON-GAUSSIAN NON-STATIONARY experimental investigation
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