Alpine plants possess unique traits to adapt alpine environments.Whether leaf trait relationships of alpine plants can be captured by the two trait dimensions of organ size and resource economics is unknown.We hypothe...Alpine plants possess unique traits to adapt alpine environments.Whether leaf trait relationships of alpine plants can be captured by the two trait dimensions of organ size and resource economics is unknown.We hypothesized that,beyond the trait dimensions of leaf size and resource economics,nonstructured carbohydrates(NSC)would reflect a dimension of cold-tolerance in alpine plants.To test this hypothesis,we measured 12 leaf traits critical to leaf construction and growth in 143 species across 7 sites ranging from alpine steppes to alpine meadows along an environmental gradient on the Tibetan Plateau.Furthermore,a cold resistance experiment was conducted at one of these sites to estimate the lethal temperature causing 50%frost damage(LT_(50))of 11 alpine species.The majority of variations in 12 leaf traits of alpine plants were captured by three trait axes,in which leaf carbon(LCC)and NSC(including leaf starch;LSC and leaf soluble sugars;LSS)were clustered in a new dimension(PC3)beyond leaf size and structure,and resource economics.Although LCC,LSC and LSS all showed negative correlations with mean annual temperature,a significant negative correlation was only found between LSS and LT_(50).It indicated that PC3 was able to reflect the cold-tolerance of alpine plants to some extent,in which LSS was the most critical trait.The storage and transformation of NSC under stressful conditions could reflect a dimension of long-term metabolic adaptation and cold-tolerance,which is an extension of the resource-utilization strategy beyond construction cost and growth.展开更多
为了明确绣球〔Hydrangea macrophylla(Thunb.)Ser.〕品种的耐寒性及筛选耐寒性评价主要指标,对40个绣球品种的低温半致死温度(LT_(50))进行比较和耐寒性分级,在此基础上,对5℃、0℃、-5℃、-10℃、-15℃、-20℃下耐寒品种‘小町’(‘Ko...为了明确绣球〔Hydrangea macrophylla(Thunb.)Ser.〕品种的耐寒性及筛选耐寒性评价主要指标,对40个绣球品种的低温半致死温度(LT_(50))进行比较和耐寒性分级,在此基础上,对5℃、0℃、-5℃、-10℃、-15℃、-20℃下耐寒品种‘小町’(‘Komachi’)、‘佳澄’(‘Kasumi’)、‘无尽夏’(‘Endless Summer’)和不耐寒品种‘甜蜜幻想’(‘Sweet Fantasy’)、‘灵感’(‘Inspiration’)、‘你我的情感’(‘You and Me Feelings’)叶的抗氧化酶活性及丙二醛(MDA)和渗透调节物质含量进行比较,并对这些耐寒指标进行相关性分析和主成分分析。结果表明:供试绣球品种LT 50的波动较大,变化范围为-36.68℃~-1.87℃,其中,‘小町’的LT_(50)最低,‘佳澄’、‘平瓣’(‘Flat’)、‘蒂亚娜’(‘Tijana’)、‘塞尔玛’(‘Selma’)、‘含羞叶’(‘Elbtal’)、‘花手鞠’(‘Stockings’)、‘蓝色多瑙河’(‘Blue Danube’)和‘无尽夏’的LT_(50)均低于-20℃。40个绣球品种的耐寒性被分成不耐寒、较耐寒、耐寒和强耐寒4个等级,分别包含13、18、8和1个品种。6个代表品种的过氧化氢酶(CAT)、多酚氧化酶(PPO)和超氧化物歧化酶活性及MDA、脯氨酸(Pro)、可溶性蛋白质(SP)和可溶性糖含量随温度降低基本呈先升高后降低的变化趋势。相关性分析结果表明PPO活性和Pro含量与LT_(50)呈极显著(P<0.01)负相关。主成分分析结果表明:前4个主成分的累计贡献率为81.212%,说明这4个主成分基本能够反映绣球品种的耐寒性;PPO活性、CAT活性、Pro含量和SP含量的负荷量绝对值分别在第1、第2、第3和第4主成分中最高。研究结果显示:供试绣球品种间的耐寒性差异较大,‘小町’等9个品种的耐寒性较强,能够在冬季温度偏低地区露天栽培,并可作为绣球耐寒品种选育的亲本。PPO活性、CAT活性、Pro含量和SP含量为绣球耐寒性评价的主要指标,尤其是PPO活性和Pro含量。展开更多
基金supported by the National Natural Science Foundation of China(32192461,32271619 and 32160285)the Natural Science Foundation of Science&Technology Department of Qinghai(2020-ZJ-952Q).
文摘Alpine plants possess unique traits to adapt alpine environments.Whether leaf trait relationships of alpine plants can be captured by the two trait dimensions of organ size and resource economics is unknown.We hypothesized that,beyond the trait dimensions of leaf size and resource economics,nonstructured carbohydrates(NSC)would reflect a dimension of cold-tolerance in alpine plants.To test this hypothesis,we measured 12 leaf traits critical to leaf construction and growth in 143 species across 7 sites ranging from alpine steppes to alpine meadows along an environmental gradient on the Tibetan Plateau.Furthermore,a cold resistance experiment was conducted at one of these sites to estimate the lethal temperature causing 50%frost damage(LT_(50))of 11 alpine species.The majority of variations in 12 leaf traits of alpine plants were captured by three trait axes,in which leaf carbon(LCC)and NSC(including leaf starch;LSC and leaf soluble sugars;LSS)were clustered in a new dimension(PC3)beyond leaf size and structure,and resource economics.Although LCC,LSC and LSS all showed negative correlations with mean annual temperature,a significant negative correlation was only found between LSS and LT_(50).It indicated that PC3 was able to reflect the cold-tolerance of alpine plants to some extent,in which LSS was the most critical trait.The storage and transformation of NSC under stressful conditions could reflect a dimension of long-term metabolic adaptation and cold-tolerance,which is an extension of the resource-utilization strategy beyond construction cost and growth.
文摘为了明确绣球〔Hydrangea macrophylla(Thunb.)Ser.〕品种的耐寒性及筛选耐寒性评价主要指标,对40个绣球品种的低温半致死温度(LT_(50))进行比较和耐寒性分级,在此基础上,对5℃、0℃、-5℃、-10℃、-15℃、-20℃下耐寒品种‘小町’(‘Komachi’)、‘佳澄’(‘Kasumi’)、‘无尽夏’(‘Endless Summer’)和不耐寒品种‘甜蜜幻想’(‘Sweet Fantasy’)、‘灵感’(‘Inspiration’)、‘你我的情感’(‘You and Me Feelings’)叶的抗氧化酶活性及丙二醛(MDA)和渗透调节物质含量进行比较,并对这些耐寒指标进行相关性分析和主成分分析。结果表明:供试绣球品种LT 50的波动较大,变化范围为-36.68℃~-1.87℃,其中,‘小町’的LT_(50)最低,‘佳澄’、‘平瓣’(‘Flat’)、‘蒂亚娜’(‘Tijana’)、‘塞尔玛’(‘Selma’)、‘含羞叶’(‘Elbtal’)、‘花手鞠’(‘Stockings’)、‘蓝色多瑙河’(‘Blue Danube’)和‘无尽夏’的LT_(50)均低于-20℃。40个绣球品种的耐寒性被分成不耐寒、较耐寒、耐寒和强耐寒4个等级,分别包含13、18、8和1个品种。6个代表品种的过氧化氢酶(CAT)、多酚氧化酶(PPO)和超氧化物歧化酶活性及MDA、脯氨酸(Pro)、可溶性蛋白质(SP)和可溶性糖含量随温度降低基本呈先升高后降低的变化趋势。相关性分析结果表明PPO活性和Pro含量与LT_(50)呈极显著(P<0.01)负相关。主成分分析结果表明:前4个主成分的累计贡献率为81.212%,说明这4个主成分基本能够反映绣球品种的耐寒性;PPO活性、CAT活性、Pro含量和SP含量的负荷量绝对值分别在第1、第2、第3和第4主成分中最高。研究结果显示:供试绣球品种间的耐寒性差异较大,‘小町’等9个品种的耐寒性较强,能够在冬季温度偏低地区露天栽培,并可作为绣球耐寒品种选育的亲本。PPO活性、CAT活性、Pro含量和SP含量为绣球耐寒性评价的主要指标,尤其是PPO活性和Pro含量。
