Recognition mechanism and sequence optimization of organophosphorus pesticides aptamers for better monitoring contaminations in food

Aptamers as a kind of biological recognition element have shown great potential in monitoring and the rapid quantification of organophosphorus pesticides(OPPs). However, molecules of OPPs are structurally similar and original aptamers selected by systematic evolution of ligands by exponential enrichment are usually long-chain bases, which hamper the further application under OPPs-aptamer recognition. The aim of the research was to develop a new strategy to design oligonucleotide sequences for binding OPPs by combination of experimental and molecular modeling methods. 3D models of aptamers binding OPPs were constructed, and binding energy and the most probable binding site for the OPPs were then determined by molecular docking, and the binding sites were further confirmed by the results of 2-AP replaced experiments. Based on the docking results, a new aptamer for detection 4 representative OPPs with only 29 bases was designed by reasonable truncation and mutation of the reported aptamer(named S4-29). The interaction between this new aptamer and OPPs were analyzed by molecular docking, microscale thermophoresis, circular dichroism and fluorometric analysis. The results revealed that the new aptamer exhibit more superior recognition performance to OPPs, which can be promote the monitoring ability of OPPs contaminations in food.

A priliminary study on the sequence characteristics and focal mechanism solution of the Jiashi strong earthquake swarm

The sequence characteristics and focal mechanism solution of the Jiashi, Xinjiang strong earthquake swarm are analyzed and studied in this paper. The result shows that before the M S=6.6 earthquake, value h of sequence frequency attenuation coefficient was less than 1, then value h was more than 1. Before occurrence of M S6.0 earthquakes the energy is released either in a continuously strengthened way or a sharply strengthened way, and before M S5.0 earthquakes the sequence frequency shows calm. The study on the focal mechanism solution of the strong earthquake swarm shows that the source faults are mainly in a right lateral, strike slip way and the faults have characteristics of tensor shear.

Research on the Source Parameters and Correlation Coefficients of Focal Mechanisms for the Yingjiang Earthquake Sequences in 2008

The source parameters of the Yingjiang earthquake sequences in 2008 are obtained by applying spectral analysis and Brunes source model,based on the digital waveform data recorded by the Yunnan Digital Seismic Network.The correlation coefficients are calculated using the low-frequency spectral amplitudes of 2 events recorded by a same station,then,events with similar focal mechanism are grouped using the clustering analysis method.Compared to the obtained focal mechanisms,it is found that there are good correlations with the azimuth of P axes in each clustering group,and the larger the correlation coefficient,the closer the azimuths of P axes.We divide the Yingjiang area into 3 regions to analyze the stress level and stress direction by combining the source parameters and the mean focal mechanism of each group.The results show:The change and transformation of the focal mechanism types at different stages can represent the temporal characteristics of the regional stress field.If the earthquake focal mechanism types are concentrated in a time period and switch to the direction of regional stress field,it may be a sign of strong earthquake.There is some relationship between the stress drop and the type of focal mechanism.Those earthquakes with stress fields revealed by focal mechanism types closer to the regional tectonic stress field will have higher stress drop,while those with the focal mechanism-revealed stress fields differing a lot from the regional tectonic stress field will generally have a lower stress drop.

Research on Stress Drops and the Focal Mechanisms of the Xinyuan- Hejing ML6.8 Earthquake Sequences

Based on the digital waveform data recorded by Xinjiang Digital Seismic Network for the Xinyuan-Hejing M_L6.8 earthquake sequences of June 30,2012,this paper analyzes the stress drops of earthquake sequences and the correlation coefficients of focal mechanisms significant for strong aftershocks.Firstly,the source parameters of the Xinyuan-Hejing M_L6.8 earthquake sequences are obtained by applying the spectrum analysis and the Brunes source model.Then,the correlation coefficients of spectral amplitudes are calculated using the low-frequency spectral amplitude recorded by the same station for the different events.Finally,based on the results of the correlation coefficients of spectral amplitudes,the events with similar focal mechanisms are grouped using the clustering method.The results show that:(1)The stress drop values show a steady trend in the aftershock sequence calm period and the stress drop values show a rise-fall in strong aftershocks.(2)The moving average correlation coefficient of amplitude spectrum begins to spread after the main shock.It shows that the correlation decreases between the main shock and the aftershocks in mechanisms.(3)The results of focal mechanism groups show that the earthquake sequences are mainly strike slips.The stress distribution of the main pressure axis is nearly NS,which is the same as the structural stress field.(4)The magnitude and mechanism show that there is an agreement before the strong aftershock,which shows that the regional stress field is enhanced.

Video expression recognition based on frame-level attention mechanism

Facial expression recognition(FER) in video has attracted the increasing interest and many approaches have been made.The crucial problem of classifying a given video sequence into several basic emotions is how to fuse facial features of individual frames.In this paper, a frame-level attention module is integrated into an improved VGG-based frame work and a lightweight facial expression recognition method is proposed.The proposed network takes a sub video cut from an experimental video sequence as its input and generates a fixed-dimension representation.The VGG-based network with an enhanced branch embeds face images into feature vectors.The frame-level attention module learns weights which are used to adaptively aggregate the feature vectors to form a single discriminative video representation.Finally, a regression module outputs the classification results.The experimental results on CK+and AFEW databases show that the recognition rates of the proposed method can achieve the state-of-the-art performance.

基于改进Sequence-to-Sequence模型的文本摘要生成方法

基于循环神经网络和注意力机制的Sequence-to-Sequence模型神经网络方法在信息抽取和自动摘要生成方面发挥了重要作用。然而,该方法不能充分利用文本的语言特征信息,且生成结果中存在未登录词问题,从而影响文本摘要的准确性和可读性。为此,利用文本语言特征改善输入的特性,同时引入拷贝机制缓解摘要生成过程未登录词问题。在此基础上,提出基于Sequence-to-Sequence模型的新方法 Copy-Generator模型,以提升文本摘要生成效果。采用中文摘要数据集LCSTS为数据源进行实验,结果表明所提方法能够有效地提高生成摘要的准确率,可应用于自动文本摘要提取任务。

Geometry and tectonic deformation of the seismogenic structure for the 8 August 2017 M_S 7.0 Jiuzhaigou earthquake sequence,northern Sichuan, China

To reveal the geometry of the seismogenic structure of the Aug. 8, 2017 M_S 7.0 Jiuzhaigou earthquake in northern Sichuan,data from the regional seismic network from the time of the main event to Oct. 31, 2017 were used to relocate the earthquake sequence by the tomoDD program, and the focal mechanism solutions and centroid depths of the M_L ≥ 3.5 events in the sequence were determined using the CAP waveform inversion method. Further, the segmental tectonic deformation characteristics of the seismogenic faults were analyzed preliminarily by using strain rosettes and areal strains(As). The results indicate:(1) The relocated M_S 7.0 Jiuzhaigou earthquake sequence displays a narrow ～ 38 km long NNW-SSE-trending zone between the NW-striking Tazang Fault and the nearly NSstriking Minjiang Fault, two branches of the East Kunlun Fault Zone. The spatial distribution of the sequence is narrow and deep for the southern segment, and relatively wide and shallow for the northern segment. The initial rupture depth of the mainshock is 12.5 km, the dominant depth range of the aftershock sequence is between 0 and 10 km with an average depth of 6.7 km. The mainshock epicenter is located in the middle of the aftershock region, showing a bilateral rupture behavior. The centroid depths of 32 M_L ≥ 3.5 events range from 3 to 12 km with a mean of about 7.3 km, consistent with the predominant focal depth of the whole sequence.(2) The geometric structure of the seismogenic fault on the southern section of the aftershock area(south of the mainshock) is relatively simple, with overall strike of ～150° and dip angle ～75°, but the dip angle and dip-orientation exhibit some variation along the segment. The seismogenic structure on the northern segment is more complicated; several faults, including the Minjiang Fault, may be responsible for the aftershock activities. The overall strike of this section is ～159° and dip angle is ～59°, illustrating a certain clockwise rotation and a smaller dip angle than the southern segment. The differences between the two segments demonstrate variation of the geometric structure along the seismogenic faults.(3) The focal mechanism solutions of 32 M_L ≥ 3.5 events in the earthquake sequence have obvious segmental characteristics. Strike-slip earthquakes are dominant on the southern segment, while 50% of events on the northern segment are thrusting and oblique thrusting earthquakes, revealing significant differences in the kinematic features of the seismogenic faults between the two segments.(4) The strain rosettes for the mainshock and the entire sequence of 31 M_L ≥ 3.5 aftershocks correspond to strike-slip type with NWW-SEE compressional white lobes and NNE-SSW extensional black lobes of nearly similar size. The strain rosette and As value of the entire sequence of 22 M_L ≥ 3.5 events on the southern segment are the same as those of the M_S 7.0 mainshock,indicating that the tectonic deformation here is strike-slip. However, the strain rosette of the entire sequence of 10 M_L ≥ 3.5 events on the northern segment show prominent white compressional lobes and small black extensional lobes, and the related As value is up to 0.52,indicating that the tectonic deformation of this segment is oblique thrusting with a certain strike-slip component. Differences between the two segments all reveal distinctly obvious segmental characteristics of the tectonic deformation of the seismogenic faults for the Jiuzhaigou earthquake sequence.

The genetic environmental transformation mechanism of coal and oil shale deposits in eastern China’s continental fault basins and the developmental characteristics of the area’s symbiotic assemblages——taking Huangxian Basin as an example

Coal and oil shale are two common sedimentary energy sources which are often symbiotically developed in M esozoic- Cenozoic continental fault basins. However, the mechanisms and characteristics of the symbiotic development are not yet clearly known. In this research study, the typical continental fault basins of eastern China were chosen as examples for the purpose of conducting an examination of the coal and oil shale symbiotic assemblage types, genetic environmental differences, and transformation mechanisms, as well as the development and occurrence characteristics o f different assemblage types. Through a large number of investigations, systematic experimental testing, and sequence stratigraphy studies, the following conclusions were obtained:(1) There were five types of coal and oil shale symbiotic assemblages observed in the continental fault basins,(2) The development of coal and oil shale deposits requires a warm and humid climate, stable structure, abundant organic matter supply, a certain water depth, and a lower terrestrial source debris supply. The observed differences were that the water depth conditions were diversified in the study area, as well as the sources, types, and content of the organic matter.(3) The rapid transformations of the coal and oil shale genetic environments were mainly controlled by the tectonic settings and climatic conditions, which were determined to control the changes in the water depths, salinity,redox conditions, and lake productivity of the genetic environments. Also, in the symbiotic assemblages, genetic environment changes had induced the development of oil shale deposits, which gradually evolved into coal genetic environments.(4) In the isochronous sequence stratigraphic framework of the coal and oil shale symbiotic assemblages, the lake expansion system tracts (EST) were determined to be the most beneficial to the growth o f all the types of assemblages and were characterized by more assemblage development phases and smaller bed thicknesses. From the early to the late stages of the EST, and from the lakesides to lake centers, the thicknesses of the coal seams in the symbiotic assemblages showed trends of thinning, while the thicknesses of the oil shale deposits exhibited increasing trends. The early stages of high stand system tracts were found to be beneficial to the development of the symbiotic assemblages of coal seams overlying the oil shale. This tract type generally presented large bed thicknesses and distribution ranges. The low stand system tract and the late high stand system tract were determined to be unconducive to the development of the symbiotic assemblages.

THE EFFECT OF BLENDING SEQUENCE ON PHASE MORPHOLOGY OF NYLON 6/ABS/SMA BLENDS

The preparation process-dependent phase morphology of blends composed of nylon 6 and acrylonitrile-butadiene- styrene(ABS)over a composition range of 30-70 wt% using a styrene-maleic anhydride(SMA)copolymer as the compatibilizing agent with a constant content(5phr)was investigated.The results of the scanning electron microscope (SEM)observation revealed that compared with the binary blends of nylon 6 and ABS,the existence of SMA caused a composition shift of phase inversion to a higher weight fraction of ny...

Modeling mechanism of a novel fractional grey mode based on matrix analysis

To fully display the modeling mechanism of the novelfractional order grey model （FGM （q,1））, this paper decomposesthe data matrix of the model into the mean generation matrix, theaccumulative generation matrix and the raw data matrix, whichare consistent with the fractional order accumulative grey model（FAGM （1,1））. Following this, this paper decomposes the accumulativedata difference matrix into the accumulative generationmatrix, the q-order reductive accumulative matrix and the rawdata matrix, and then combines the least square method, findingthat the differential order affects the model parameters only byaffecting the formation of differential sequences. This paper thensummarizes matrix decomposition of some special sequences,such as the sequence generated by the strengthening and weakeningoperators, the jumping sequence, and the non-equidistancesequence. Finally, this paper expresses the influences of the rawdata transformation, the accumulation sequence transformation,and the differential matrix transformation on the model parametersas matrices, and takes the non-equidistance sequence as an exampleto show the modeling mechanism.

Accuracy Analysis for 6-DOF PKM with Sobol Sequence Based Quasi Monte Carlo Method

To improve the precisions of pose error analysis for 6-dof parallel kinematic mechanism( PKM)during assembly quality control,a Sobol sequence based on Quasi Monte Carlo( QMC) method is introduced and implemented in pose accuracy analysis for the PKM in this paper. The Sobol sequence based on Quasi Monte Carlo with the regularity and uniformity of samples in high dimensions,can prevail traditional Monte Carlo method with up to 98. 59% and 98. 25% enhancement for computational precision of pose error statistics.Then a PKM tolerance design system integrating this method is developed and with it pose error distributions of the PKM within a prescribed workspace are finally obtained and analyzed.

Spatiotemporal evolution of the 2021 M_(S)6.4 Yangbi earthquake sequence

Based on the seismic phase reports of the Yangbi area from January 1 to June 25,2021,and the waveform data of M≥4 earthquakes,we obtained the relocation results and focal mechanism solutions of the M_(S)6.4 Yangbi earthquake sequence using the HypoDD and CAP methods.Based on our results,our main conclusions are as follows:(1)the M_(S)6.4 Yangbi earthquake sequence is a typical foreshock-mainshock-aftershock sequence.The fore-shocks of the first two stages have the obvious fronts of migration and their migration rate increased gradually.There was no apparent front of migration during the third stage,and the occurrence of the mainshock was related to stress triggering from a M5.3 foreshock.We tentatively speculate that the rupture pattern of the Yangbi earthquake sequence conforms to the cascading-rupture model;and(2)the main fault of the M_(S)6.4 Yangbi earthquake sequence is a NW-trending right-lateral strike-slip fault.As time progressed,a minor conjugate aftershock belt formed at the northwest end of this fault,and a dendritic branching structure emerged in the southern fault segment,showing a complex seismogenic fault structure.We suggested that the fault of the Yangbi earthquake sequence may be a young sub-fault of the Weixi-Weishan fault.

Comparison and analysis of the stress field in the source region of Tangshan and Lancang earthquake sequences

The state of the stress fields in the source region is analysed systematically, on the basis of the focal mechanismsof 167 events with Ms≥4. 0 of Tangshan sequence and 163 moderate-small events of Lancang sequence respectively. The result indicates that the directions of the stress field in the source region are generally stable and thevariation is not obvious after the occurrence of strong shock. The dominant orientations of focal mechanisms ofthe aftershocks are consistent with that of the mainshock, there is still a small difference between some mechanisms of aftershocks and the mechanism of mainshock, and the difference decreases as time goes on. The numbers of solutions with dominant direction of Tangshan and Lancang sequences are similar to each other, it indicates that the controls of the strong earthquake sequences from the tectonic stress field are similar to each othertoo. Through the hierarchical clustering analysis of focal mechanism,it is found that the number of clusters ofTangshan sequence is larger than that of Lancang sequence,and their orientations are relatively disordered. Itmay be the cause that the tectonics in the Tangshan region are more complicated than those in the Lancang region.

STUDIES ON COPOLYCONDENSATION MECHANISMS FROM m-METHOXY-p-ACETOXYBENZOIC ACID,p-ACETOXYBENZOIC ACID AND POLY(ETHYLENE TEREPHTHALATE)

Copolycondensation mechanisms for the terpolyester system from m-methoxy-p-acetoxybenzoic acid,p-acetoxybenzoic acid and poly(ethylene terephthalate)are first investigatedby high resolution proton NMR spectroscopy at 400 MHz.A series of reactivity ratios of thecopolycondensation reactions are given.The results shown that the m-methoxy-p-acetoxybenzoicacid tends to homopolymerize,while the p-acetoxybenzoic acid,and the ethylene terephthalate unitespecially,tend to copolymerize with the m-methoxy-p-acetoxybenzoic acid.

The fracture characteristic of the 1989 Datong-Yanggao earthquake sequence

This paper, first, analyzed the seismic activity patterns of the Datong-Yanggao earthquake sequence by 4 stages,finding that three times earthquakes of Mis were located in a same NNE seismically active zone and two times located in 2 NWW seismically active zones. Then the focal-mechanism solutions of 12 earthquakes of M≥4 and 114 earthquakes of M≥1. 0 in various zones were obtained, a tectonic analysis was made for these data, the obtained fracture characteristic of this sequence is: the conjugate fracture combination with the NNE right lateral faults as its trunk and the two NWW left lateral faults as its branches. Finally, it is pointed out that there is an obvious difference between the seismic fracture and the tensile dip-slip tectonic activity of the main body of the seismic region.

A possible hydrolysis mechanism of β-naphthyl acetate catalyzed by antibodies

The mechanism of ester hydrolysis has been extensively studied; however, the precise function of active-site residues in promoting catalysis is nuclear. We describe here the structural models for the complex of a catalytic sntibody Fv fragment with a phosphonate transition -state analogue, constructed by using gene cloning, sequencing and molecular modeling, mainly based on a known X-ray structure of a catalytic atibody. Hydrophobic and electrostatic analyses of the Fv/analog and Fv/substrate interaction suggest the hydrolysis mechanism: In L91 and Tyr H97 play important roles to stabilize the β-naphthyl group of hapten through r-stack; His H35 donates a pair of free electrons at the atom NEZ to an active water and let it to be a partial hydroxide, which attacks the carbon atom of the carbonyl group of the substrate. Both His H35 and Arg L96 can form hydrogen bonds and stabilize the Anoinc tetrahedral intermediate formed during turnover. This mechanism emphasizes that an active water bridge may be formed during hydrolysis process.

Conjugation with Acridines Turns Nuclear Localization Sequence into Highly Active Antimicrobial Peptide

The emergence of multidrug-resistant bacteria creates an urgent need for alternative antibiotics with new mechanisms of action. In this study, we synthesized a novel type of antimicrobial agent, Ac r_3-NLS, by conjugating hydrophobic acridines to the N-terminus of a nuclear localization sequence(NLS), a short cationic peptide. To further improve the antimicrobial activity of our agent, dimeric(Acr_3-NLS)_2 was simultaneously synthesized by joining two monomeric Acr_3-NLS together via a disulfide linker. Our results show that Acr_3-NLS and especially(Acr_3-NLS)_2 display signifi cant antimicrobial activity against gramnegative and gram-positive bacteria compared to that of the NLS. Subsequently, the results derived from the study on the mechanism of action demonstrate that Acr_3-NLS and(Acr_3-NLS)_2 can kill bacteria by membrane disruption and DNA binding. The double targets—cell membrane and intracellular DNA—will reduce the risk of bacteria developing resistance to Acr_3-NLS and(Acr_3-NLS)_2. Overall, this study provides a novel strategy to design highly eff ective antimicrobial agents with a dual mode of action for infection treatment.

A Review of Aftershock Mechanism Research

In this paper,research of aftershock mechanisms is reviewed,including heterogeneity of medium and stress,mechanical loading,fluid intrusion and stress corrosion,and ratestate dependence.Previous studies have indicated that the heterogeneity of media and stress is the basic premise of aftershocks generated.From the point view of mechanics,transient creep and afterslip can explain the decay of aftershocks in a short time after a mainshock and the relaxation of stress tends to interpret the characteristics of long-term aftershocks.Fluid intrusion and stress corrosion control the evolution process of the aftershocks under certain conditions.The interaction between the faults perturbed by the mainshock always exists during the aftershock activities.All kinds of models and the theories want to comply with the two basic power-law relationships---the G-R law and Omori law to some extent.

The Focal Mechanism Solutions of the M_S 8.0 Wenchuan Earthquake in Sichuan on May 12,2008 and Some of Its Aftershocks

The focal mechanism solutions of the Wenchuan earthquake （Ms8.0） of May 12, 2008 and some of its aftershocks occurring up to December I0, 2008 are determined with lower semisphere of equal-projection and first motion sign data of P waves from regional and distant stations. The focal mechanism solutions of the Ms8.0 Wenchuan earthquake are： Nodal plane I：strike 5°, dip angle 48°, slip angle 39°; Nodal plane II： strike 247°, dip angle 62°, slip angle 131°; P axis azimuth 309°, plunge 8°, T axis azimuth 208°, plunge 54°, B axis azimuth 44°, plunge 35% Combining geological tectonics and spatial distribution of aftershocks, nodal plane II can be identified as a seismogenic fault. According to focal mechanism solutions, the fault activity that triggered the huge earthquake is reverse thrusting. The main rupture surface is S67°W, basically identical to the fault strike on which the earthquake occurred. The main compression stress P axis is N51°W, which is basically the same as the direction of the regional tectonic stress field. According to the results of focal mechanism solutions of aftershocks, the aftershocks occurring in the southern and northern sections of the Longmenshan fault zone have predominant orientations and are obviously different. For the main shock and the early aftershocks occurring on the southern section of the Longmenshan fault, the rupturing is mainly characterized by reverse-dip slip with some strike-slip, and over time, the aftershocks migrated towards the northern section. The rupturing in the source is mainly characterized by strike-slip with some reverse-dip slips. The stress field is controlled by the main shock stress field in the southern section of the Longmenshan tectonic zone, while it is controlled by the main shock stress field and regional stress field in the northern section of the Longmenshan tectonic zone.