目的为青牛胆的物种鉴定和遗传图谱的构建寻找一种新的途径。方法采用序列相关扩增多态性(Sequence related amplified polymorphism,SRAP)技术对青牛胆DNA进行PCR扩增,逐级优化反应参数。结果优化得到稳定重复性好的青牛胆SRAP反应体...目的为青牛胆的物种鉴定和遗传图谱的构建寻找一种新的途径。方法采用序列相关扩增多态性(Sequence related amplified polymorphism,SRAP)技术对青牛胆DNA进行PCR扩增,逐级优化反应参数。结果优化得到稳定重复性好的青牛胆SRAP反应体系。结论SRAP技术在分子水平上对青牛胆进行鉴定是一种行之有效的手段,为今后进一步的青牛胆物种鉴定、遗传图谱的构建等研究奠定基础。展开更多
Retrotransposons are present in all plant genomes and play important roles in genome size,genome structure remodeling,gene function and genome evolution.Me07Em02 marked by sequence-related amplified polymorphism (SRAP...Retrotransposons are present in all plant genomes and play important roles in genome size,genome structure remodeling,gene function and genome evolution.Me07Em02 marked by sequence-related amplified polymorphism (SRAP) is verified,recovered,sequenced and analyzed,which closely link to the flesh color around the stone.By comparison with the peach genome Peach v1.0,a part of the sequence of copia-like retrotransposons is obtained.With the aid of DANMAN and DNASTAR sequence analysis software,BLASTn alignment is carried out in the peach genome database and GenBank.The whole genome sequence of the copialike retrotransposon in the peach is located at 9 939 764~9 944 771 bp in Scaffold-1 of Peach v1.0,with a total length of 5 008 bp.The LTR on both sides is exactly the same,the length is 444 bp,and the largest ORF box is located at the 487~4 545 bp of the sequence,encoding 1 535 amino acids.The amino acid sequence was submitted to GenBank for Protein BLAST alignment,The results showed that this sequence also has the typical amino acid sequence characteristics of the copia-like retrotransposon.At the same time,the method of peach retrotransposon labeling is preliminarily established,and the genetic diversity of the peach is analyzed.展开更多
Using SRAP(sequence-related amplified polymorphism)markers a genetic linkage map of cucumber was constructed with a population consisting of 138 F_(2) individuals derived from a cross of the two cucumber lines,S06 and...Using SRAP(sequence-related amplified polymorphism)markers a genetic linkage map of cucumber was constructed with a population consisting of 138 F_(2) individuals derived from a cross of the two cucumber lines,S06 and S52.In the survey of parental polymorphisms with 182 primer combinations,64 polymorphism-revealing primer pairs were screened out,which generated totally 108 polymorphic bands with an average of 1.7 bands per primer pair and at most 6 bands from one primer pair.The constructed molecular linkage map included 92 loci,distributed in seven linkage groups and spanning 1164.2 cM in length with an average genetic distance of 12.6 cM between two neighboring loci.Based on this linkage map,the quantitative trait loci(QTL)for the lateral branch number(lbn)and the lateral branch average length(lbl)in cucumber were identified by QTLMapper1.6.A major QTL lbn1 located between ME11SA4B and ME5EM5 in LG2 could explain 10.63%of the total variation with its positively effecting allele from S06.A major QTL lbl1 located between DC1OD3 and DC1EM14 in LG2 could account for 10.38%of the total variation with its positively effecting allele from S_(06).展开更多
Barley(Hordeum vulgare L.)is one of the most Aluminum(Al)sensitive cereal species.In this study,the physiological,biochemical,and molecular response of barley seedlings to Al treatment was examined to gain insight int...Barley(Hordeum vulgare L.)is one of the most Aluminum(Al)sensitive cereal species.In this study,the physiological,biochemical,and molecular response of barley seedlings to Al treatment was examined to gain insight into Al response and tolerance mechanisms.The results showed that superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)activity were inhibited to different degrees following Al exposure.The MDA content also significantly increased with increasing Al concentrations.SRAP results indicated significant differences between Al treatments and controls in terms of SRAP profile,and the genomic template stability(GTS)decreased with increasing Al concentration and duration.These integrative results help to elucidate the underlying mechanisms that the barley response to Al toxicity.展开更多
A genetic linkage map of cotton was constructed with a newly developed molecular marker-SRAP (sequence-related amplified polymorphism) using a population consisting of 129 F2 individuals derived from the interspecific...A genetic linkage map of cotton was constructed with a newly developed molecular marker-SRAP (sequence-related amplified polymorphism) using a population consisting of 129 F2 individuals derived from the interspecific cross of Handan208 Pima90. A total of 136 primer pairs were used to detect polymorphisms between the two parents and 76 primer pairs with better polymorphisms were picked out to analyze the F2 population. 285 polymorphic bands were generated in total with an average of 3.75 polymorphic bands per pair of primers. The primer pair showing most polymorphic bands was the combination of me3 and em2, which produced 13 polymorphic bands. The 285 loci were used to construct linkage map with MAPMAKER/EXP3.0 and 237 loci were mapped at a LOD≥3.0 on 39 linkage groups. The total length of the map is 3030.7 cM, covering 65.4% of the whole cotton genome, and the average distance between adjacent markers is 12.79 cM. All the markers are distributed evenly among the linkage groups without clustering of loci. This is the first linkage map of cotton comprised of SRAP markers.展开更多
文摘目的为青牛胆的物种鉴定和遗传图谱的构建寻找一种新的途径。方法采用序列相关扩增多态性(Sequence related amplified polymorphism,SRAP)技术对青牛胆DNA进行PCR扩增,逐级优化反应参数。结果优化得到稳定重复性好的青牛胆SRAP反应体系。结论SRAP技术在分子水平上对青牛胆进行鉴定是一种行之有效的手段,为今后进一步的青牛胆物种鉴定、遗传图谱的构建等研究奠定基础。
文摘Retrotransposons are present in all plant genomes and play important roles in genome size,genome structure remodeling,gene function and genome evolution.Me07Em02 marked by sequence-related amplified polymorphism (SRAP) is verified,recovered,sequenced and analyzed,which closely link to the flesh color around the stone.By comparison with the peach genome Peach v1.0,a part of the sequence of copia-like retrotransposons is obtained.With the aid of DANMAN and DNASTAR sequence analysis software,BLASTn alignment is carried out in the peach genome database and GenBank.The whole genome sequence of the copialike retrotransposon in the peach is located at 9 939 764~9 944 771 bp in Scaffold-1 of Peach v1.0,with a total length of 5 008 bp.The LTR on both sides is exactly the same,the length is 444 bp,and the largest ORF box is located at the 487~4 545 bp of the sequence,encoding 1 535 amino acids.The amino acid sequence was submitted to GenBank for Protein BLAST alignment,The results showed that this sequence also has the typical amino acid sequence characteristics of the copia-like retrotransposon.At the same time,the method of peach retrotransposon labeling is preliminarily established,and the genetic diversity of the peach is analyzed.
基金This work was support ed by the Shanghai Municipal Scientifi c&Technological Commission(Grant Nos.033107019)Shanghai Municipal Agricultural Commission(Grant Nos 2003-2-1-2).
文摘Using SRAP(sequence-related amplified polymorphism)markers a genetic linkage map of cucumber was constructed with a population consisting of 138 F_(2) individuals derived from a cross of the two cucumber lines,S06 and S52.In the survey of parental polymorphisms with 182 primer combinations,64 polymorphism-revealing primer pairs were screened out,which generated totally 108 polymorphic bands with an average of 1.7 bands per primer pair and at most 6 bands from one primer pair.The constructed molecular linkage map included 92 loci,distributed in seven linkage groups and spanning 1164.2 cM in length with an average genetic distance of 12.6 cM between two neighboring loci.Based on this linkage map,the quantitative trait loci(QTL)for the lateral branch number(lbn)and the lateral branch average length(lbl)in cucumber were identified by QTLMapper1.6.A major QTL lbn1 located between ME11SA4B and ME5EM5 in LG2 could explain 10.63%of the total variation with its positively effecting allele from S06.A major QTL lbl1 located between DC1OD3 and DC1EM14 in LG2 could account for 10.38%of the total variation with its positively effecting allele from S_(06).
基金This research was funded by National Key Technology Research and Development Program(2015BAD01B02)the National Natural Science Foundation of China(31401316).
文摘Barley(Hordeum vulgare L.)is one of the most Aluminum(Al)sensitive cereal species.In this study,the physiological,biochemical,and molecular response of barley seedlings to Al treatment was examined to gain insight into Al response and tolerance mechanisms.The results showed that superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)activity were inhibited to different degrees following Al exposure.The MDA content also significantly increased with increasing Al concentrations.SRAP results indicated significant differences between Al treatments and controls in terms of SRAP profile,and the genomic template stability(GTS)decreased with increasing Al concentration and duration.These integrative results help to elucidate the underlying mechanisms that the barley response to Al toxicity.
基金This work was supported by the National"863"High Technology Foundation(Grant Nos.2001AA211121 and 2002AA-211031)the National"948"Foundation(Grant No.201012).
文摘A genetic linkage map of cotton was constructed with a newly developed molecular marker-SRAP (sequence-related amplified polymorphism) using a population consisting of 129 F2 individuals derived from the interspecific cross of Handan208 Pima90. A total of 136 primer pairs were used to detect polymorphisms between the two parents and 76 primer pairs with better polymorphisms were picked out to analyze the F2 population. 285 polymorphic bands were generated in total with an average of 3.75 polymorphic bands per pair of primers. The primer pair showing most polymorphic bands was the combination of me3 and em2, which produced 13 polymorphic bands. The 285 loci were used to construct linkage map with MAPMAKER/EXP3.0 and 237 loci were mapped at a LOD≥3.0 on 39 linkage groups. The total length of the map is 3030.7 cM, covering 65.4% of the whole cotton genome, and the average distance between adjacent markers is 12.79 cM. All the markers are distributed evenly among the linkage groups without clustering of loci. This is the first linkage map of cotton comprised of SRAP markers.
