In order to alleviate the flight congestion in terminal areas(TMAs),it is of great significance to develop an effective method.An arrival sequencing model based on the serial point merge systems(PMSs)is constructed to...In order to alleviate the flight congestion in terminal areas(TMAs),it is of great significance to develop an effective method.An arrival sequencing model based on the serial point merge systems(PMSs)is constructed to improve the operational benefits of arrival flights.The approach of first come first service(FCFS)combined with the method of constraint position shift(CPS)is used as the sequencing strategy.Through the simulated annealing algorithm,the results show that the arrival flights sequencing through serial PMSs has significant advantages in reducing delays and increasing runway throughput especially in the case of high traffic loads.The proposed approach is conducive in promoting the implementation and application of serial PMS.展开更多
This study examined the gene expression patterns of peripheral blood mononuclear cells （PBMCs） in patients with systemic lupus erythematosus （SLE） by using serial analysis of gene expression （SAGE） technology. F...This study examined the gene expression patterns of peripheral blood mononuclear cells （PBMCs） in patients with systemic lupus erythematosus （SLE） by using serial analysis of gene expression （SAGE） technology. Following the construction of serial analysis of gene expression （SAGE） library of PBMCs collected from 3 cases of familial SLE patients, a large scale of tag Sequencing was performed. The data extracted from sequencing files was analyzed with SAGE 2000 V 4.5 software. The top 30 expressed genes of SLE patients were uploaded to http：//david.niaid.nih.gov/david/ease.htm and the functional classification of genes was obtained. The differences among those expressed gene were analyzed by Chi-square tests. The results showed that a total of 1286 unique SAGE tags were identified from 1814 individual SAGE tags. Among the 1286 unique tags, 86.8% had single copy, and only 0.2% tags had more than 20 copies. And 68.4% of the tags matched known expressed sequences, 41.1% of which matched more than one known expressed sequence. About 31.6% of the tags had no match and could represent potentially novel genes. Approximately one third of the top 30 genes were ribosomal protein, and the rest were genes related to metabolism or with unknown functions. Eight tags were found to express differentially in SAGE library of SLE patients. This study draws a profile of gene expression patterns of PBMCs in patients with SLE. Comparison of SAGE database from PBMCs between normal individuals and SLE patients will help us to better understand the pathogenesis of SLE.展开更多
基金supported by the National Natural Science Foundation of China(No.U1933119)the Foundation of Graduate Innovation Center in Nanjing University of Aeronautics and Astronautics(No.xcxjh20210703)
文摘In order to alleviate the flight congestion in terminal areas(TMAs),it is of great significance to develop an effective method.An arrival sequencing model based on the serial point merge systems(PMSs)is constructed to improve the operational benefits of arrival flights.The approach of first come first service(FCFS)combined with the method of constraint position shift(CPS)is used as the sequencing strategy.Through the simulated annealing algorithm,the results show that the arrival flights sequencing through serial PMSs has significant advantages in reducing delays and increasing runway throughput especially in the case of high traffic loads.The proposed approach is conducive in promoting the implementation and application of serial PMS.
基金This project was supported by a grant form the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry (No [2002]247)
文摘This study examined the gene expression patterns of peripheral blood mononuclear cells （PBMCs） in patients with systemic lupus erythematosus （SLE） by using serial analysis of gene expression （SAGE） technology. Following the construction of serial analysis of gene expression （SAGE） library of PBMCs collected from 3 cases of familial SLE patients, a large scale of tag Sequencing was performed. The data extracted from sequencing files was analyzed with SAGE 2000 V 4.5 software. The top 30 expressed genes of SLE patients were uploaded to http：//david.niaid.nih.gov/david/ease.htm and the functional classification of genes was obtained. The differences among those expressed gene were analyzed by Chi-square tests. The results showed that a total of 1286 unique SAGE tags were identified from 1814 individual SAGE tags. Among the 1286 unique tags, 86.8% had single copy, and only 0.2% tags had more than 20 copies. And 68.4% of the tags matched known expressed sequences, 41.1% of which matched more than one known expressed sequence. About 31.6% of the tags had no match and could represent potentially novel genes. Approximately one third of the top 30 genes were ribosomal protein, and the rest were genes related to metabolism or with unknown functions. Eight tags were found to express differentially in SAGE library of SLE patients. This study draws a profile of gene expression patterns of PBMCs in patients with SLE. Comparison of SAGE database from PBMCs between normal individuals and SLE patients will help us to better understand the pathogenesis of SLE.
