Objective:To analyze the level changes of the degree of back pain by visual analogue scale(VAS),lumbar function(Japanese Orthopaedic Association Scores,JOA),muscle temperature,serum serotonin 5-hydroxytryptamine(5-HT)...Objective:To analyze the level changes of the degree of back pain by visual analogue scale(VAS),lumbar function(Japanese Orthopaedic Association Scores,JOA),muscle temperature,serum serotonin 5-hydroxytryptamine(5-HT)andβ-endorphin(β-EP)in patients of low back pain(LBP).Methods:64 LBP patients treated in the tuina department of first affiliated hospital of Hainan Medical University were randomly divided into observation group(32 cases)and control group(32 cases).The control group was treated with traditional filiform acupuncture needling,the observation group was treated with Chinese tuina.VAS,JOA,muscle temperature,5-HT,β-EP,these indexes were tested before and after treatment.Results:The VAS and JOA score in the observation group were significantly lower than those in the control group(P<0.01),the muscle temperature in the observation group and the control group increased significantly(P<0.01),and the observation group was higher than that in the control group(P<0.05);5-HT、β-EP was significant difference before and after treatment(P<0.01),and the level in observation group was significantly higher than that in the control group,the difference was statistically significant(P<0.05).Conclusion:Chinese tuina has significant clinical efficacy in the treatment of LBP.The medical infrared thermal imaging technology and serum pain detection technology is conducive to objectively helpful to analyze the exact efficacy of tuina in the treatment of LBP and its impact on relevant indexes.展开更多
目的:5-HT1A基因C-1019G多态性与多种神经精神疾病如抑郁症、精神分裂症、焦虑症等的发生风险、症状严重程度及治疗效果存在关联。本研究旨在建立一种直接巢式PCR结合测序检测该位点的方法。方法:以口腔上皮细胞粗处理物为材料,通过直...目的:5-HT1A基因C-1019G多态性与多种神经精神疾病如抑郁症、精神分裂症、焦虑症等的发生风险、症状严重程度及治疗效果存在关联。本研究旨在建立一种直接巢式PCR结合测序检测该位点的方法。方法:以口腔上皮细胞粗处理物为材料,通过直接巢式PCR扩增包含5-HT1A基因C-1019G位点的靶片段,PCR产物经桑格测序鉴定基因型。结果:所检样本均能扩增出预期大小的PCR产物,测序峰图清晰。结论:成功建立了一种直接巢式PCR结合测序鉴定5-HT1A基因型的方法,有良好的应用前景。Objective: The C-1019G polymorphism of the 5-HT1A gene has been associated with the risk of occurrence, symptom severity, and treatment outcome of a variety of neuropsychiatric disorders, such as depression, schizophrenia, and anxiety disorders. The aim of this study was to establish a direct nested PCR combined with sequencing to detect this locus. Methods: The target fragment containing the C-1019G locus of the 5-HT1A gene was amplified by direct nested PCR using crude processed oral epithelial cells, and the PCR product was genotyped by Sanger sequencing. Results: PCR products of expected size were amplified from all the samples tested, and the sequencing peaks were clear. Conclusion: A direct nested PCR combined with sequencing method was successfully established to identify the genotypes of 5-HT1A gene, which has good prospects for application.展开更多
Objective: To observe the influence of acupuncture stimulus and thermal stimulus on the expression of mast cells(MCs) and 5-hydroxytryptamine(5-HT) in local acupoint area of Guanyuan(关元 CV 4) and serum 5-HT in rats....Objective: To observe the influence of acupuncture stimulus and thermal stimulus on the expression of mast cells(MCs) and 5-hydroxytryptamine(5-HT) in local acupoint area of Guanyuan(关元 CV 4) and serum 5-HT in rats.Methods: Thirty-nine male C57 BL/6 rats were randomly divided into blank control group, acupuncture stimulus group and thermal stimulus group, with 13 rats in each group. The rats were stimulated by manual acupuncture at CV 4 for 5 min in acupuncture stimulus group, while those in thermal stimulus group were stimulated by adopting a thermal moxibustion apparatus on CV 4 for 30 min. The expression of MC and 5-HT in the skin in the acupoint area of CV 4 before and after acupuncture stimulus and thermal stimulus was observed and analyzed via adopting toluidine blue staining method and immunofluorescence histochemical method(5 rats were selected from each group), and the 5-HT content in serum before and after stimulus was determined through enzyme-linked immunosorbent assay(ELISA)(8 rats were selected from each group). The influence of acupuncture stimulus and thermal stimulus on the contents of MC and 5-HT in the skin and serum 5-HT in rats was analyzed and compared.Results: ① After acupuncture stimulus or thermal stimulus on CV 4 of C57 BL/6 rats, the number of MC in the acupoint area significantly increased when compared with that in blank group(the acupuncture stimulus group 12.40 ± 2.07 vs. the blank group 3.00 ±5.96;thermal stimulus group 26.20 ± 10.85 vs. the blank group 12.40 ± 2.07, both P< 0.05), and MC aggregation and degranulation were observed(the acupuncture stimulus group 17.80 ±4.55 vs. the blank group 8.00 ±3.16;the thermal stimulus group24.00±9.05 vs. the blank group 8.00±3.16. P<0.05, P< 0.01).② After acupuncture stimulus or thermal stimulus, 5-HT was released by MCs in the acupoint area, which aggregated around the blood vessels,and the number of 5-HT in the acupoint area significantly increased when compared with that in blank group(the blank group 3.00 ±1.28 vs. the acupuncture stimulus group 10.02 ±3.21;the blank group3.00 ±1.28 vs. the thermal stimulus group 14.00 ±3.94, both P< 0.01).③ Compared with blank group,both acupuncture stimulus and thermal stimulus could reduce the 5-HT content in serum(the blank group 0.72 ±0.2372 vs. acupuncture stimulus group 0.43 ±0.21: the blank group 0.72 ±0.24 vs. thermal stimulus group 0.32 ±0.18, both P<0.01), and the effect in thermal stimulus group was slightly superior to that in acupuncture stimulus group(P<0.05).Conclusion: Both acupuncture stimulus and thermal stimulus can cause the aggregation and degranulation of MCs and high expression of 5-HT in the acupoint area. The effect of thermal stimulus was superior to that of acupuncture stimulus in degranulation.展开更多
5-HT1A基因编码5-羟色胺(血清素)的G蛋白偶联受体(属于5-羟色胺受体亚家族)。C-1019G多态位点是5-HT1A基因启动子区一个重要的功能性多态位点,人群中存在3种基因型即CC、CG、GG,它与个体的恋爱关系及抑郁症、焦虑症等精神疾病密切相关...5-HT1A基因编码5-羟色胺(血清素)的G蛋白偶联受体(属于5-羟色胺受体亚家族)。C-1019G多态位点是5-HT1A基因启动子区一个重要的功能性多态位点,人群中存在3种基因型即CC、CG、GG,它与个体的恋爱关系及抑郁症、焦虑症等精神疾病密切相关。本文对5-HT1A基因C-1019G多态位点的相关机制、检测方法、研究进展等进行综述。The 5-HT1A gene encodes a G protein-coupled receptor for serotonin, which belongs to the 5-hydroxytryptamine receptor subfamily. C-1019G polymorphism is an important functional poly-morphism in the promoter region of 5-HT1A gene. There are three genotypes (CC, CG and GG) in the population, which are closely related to individual’s romantic relationship and mental disorders such as depression and anxiety. In this paper, the mechanism, detection methods and research progress of C-1019G polymorphism of 5-HT1A gene were reviewed.展开更多
Objective:To investigate the effects of electricity on the expression of procalcitonin(CGRP)and serotonin(5-HT)in serum and colon in rats with constipation-type irritable bowel syndrome(IBS-C).Methods:50 rats were ran...Objective:To investigate the effects of electricity on the expression of procalcitonin(CGRP)and serotonin(5-HT)in serum and colon in rats with constipation-type irritable bowel syndrome(IBS-C).Methods:50 rats were randomly divided into three groups:normal control group,model group,electroacupuncture group with four single points(tianshu electroacupuncture group,Colonic acupuncture group,Quchi electroacupuncture group,Shangjuxu acupoint group,and bilateral acupoint alternating between left and right),and non-meridian non-acupoint group(non-meridian non-acupoint group on both hip of electroacupuncture rats).The IBS-C model was replicated in the other three groups,except the normal control group,using the cold-fear-starvation synthesis method.After successful replication of the model,the four electroacupuncture single-point group and the non-acupoint group began electroacupuncture treatment once a day for 3 consecutive days.The visceral sensitivity and the expression of CGRP and 5-HT in serum and colon were observed.Results:Compared with the normal control group,the minimum volume threshold of abdominal retractile reflex(AWR)in the model group decreased(P<0.05),and the expressions of 5-HT and CGRP in serum and colon increased(P<0.05).Compared with the model group,AWR minimum volume threshold was increased in the electroacupuncture group with four single acupuncture points(P<0.05),the expression of 5-ht and CGRP in serum and colon was significantly decreased(P<0.05),and there was no significant change in each indicator in the non-acupuncture point group(P>0.05).Conclusion:Electroacupuncture combined with four single points can effectively improve the visceral sensitivity of IBS-C rats and regulate the levels of 5-HT and CGRP in serum and colon of rats,thus providing experimental basis for the clinical treatment of IBS-C.展开更多
文摘Objective:To analyze the level changes of the degree of back pain by visual analogue scale(VAS),lumbar function(Japanese Orthopaedic Association Scores,JOA),muscle temperature,serum serotonin 5-hydroxytryptamine(5-HT)andβ-endorphin(β-EP)in patients of low back pain(LBP).Methods:64 LBP patients treated in the tuina department of first affiliated hospital of Hainan Medical University were randomly divided into observation group(32 cases)and control group(32 cases).The control group was treated with traditional filiform acupuncture needling,the observation group was treated with Chinese tuina.VAS,JOA,muscle temperature,5-HT,β-EP,these indexes were tested before and after treatment.Results:The VAS and JOA score in the observation group were significantly lower than those in the control group(P<0.01),the muscle temperature in the observation group and the control group increased significantly(P<0.01),and the observation group was higher than that in the control group(P<0.05);5-HT、β-EP was significant difference before and after treatment(P<0.01),and the level in observation group was significantly higher than that in the control group,the difference was statistically significant(P<0.05).Conclusion:Chinese tuina has significant clinical efficacy in the treatment of LBP.The medical infrared thermal imaging technology and serum pain detection technology is conducive to objectively helpful to analyze the exact efficacy of tuina in the treatment of LBP and its impact on relevant indexes.
文摘目的:5-HT1A基因C-1019G多态性与多种神经精神疾病如抑郁症、精神分裂症、焦虑症等的发生风险、症状严重程度及治疗效果存在关联。本研究旨在建立一种直接巢式PCR结合测序检测该位点的方法。方法:以口腔上皮细胞粗处理物为材料,通过直接巢式PCR扩增包含5-HT1A基因C-1019G位点的靶片段,PCR产物经桑格测序鉴定基因型。结果:所检样本均能扩增出预期大小的PCR产物,测序峰图清晰。结论:成功建立了一种直接巢式PCR结合测序鉴定5-HT1A基因型的方法,有良好的应用前景。Objective: The C-1019G polymorphism of the 5-HT1A gene has been associated with the risk of occurrence, symptom severity, and treatment outcome of a variety of neuropsychiatric disorders, such as depression, schizophrenia, and anxiety disorders. The aim of this study was to establish a direct nested PCR combined with sequencing to detect this locus. Methods: The target fragment containing the C-1019G locus of the 5-HT1A gene was amplified by direct nested PCR using crude processed oral epithelial cells, and the PCR product was genotyped by Sanger sequencing. Results: PCR products of expected size were amplified from all the samples tested, and the sequencing peaks were clear. Conclusion: A direct nested PCR combined with sequencing method was successfully established to identify the genotypes of 5-HT1A gene, which has good prospects for application.
文摘Objective: To observe the influence of acupuncture stimulus and thermal stimulus on the expression of mast cells(MCs) and 5-hydroxytryptamine(5-HT) in local acupoint area of Guanyuan(关元 CV 4) and serum 5-HT in rats.Methods: Thirty-nine male C57 BL/6 rats were randomly divided into blank control group, acupuncture stimulus group and thermal stimulus group, with 13 rats in each group. The rats were stimulated by manual acupuncture at CV 4 for 5 min in acupuncture stimulus group, while those in thermal stimulus group were stimulated by adopting a thermal moxibustion apparatus on CV 4 for 30 min. The expression of MC and 5-HT in the skin in the acupoint area of CV 4 before and after acupuncture stimulus and thermal stimulus was observed and analyzed via adopting toluidine blue staining method and immunofluorescence histochemical method(5 rats were selected from each group), and the 5-HT content in serum before and after stimulus was determined through enzyme-linked immunosorbent assay(ELISA)(8 rats were selected from each group). The influence of acupuncture stimulus and thermal stimulus on the contents of MC and 5-HT in the skin and serum 5-HT in rats was analyzed and compared.Results: ① After acupuncture stimulus or thermal stimulus on CV 4 of C57 BL/6 rats, the number of MC in the acupoint area significantly increased when compared with that in blank group(the acupuncture stimulus group 12.40 ± 2.07 vs. the blank group 3.00 ±5.96;thermal stimulus group 26.20 ± 10.85 vs. the blank group 12.40 ± 2.07, both P< 0.05), and MC aggregation and degranulation were observed(the acupuncture stimulus group 17.80 ±4.55 vs. the blank group 8.00 ±3.16;the thermal stimulus group24.00±9.05 vs. the blank group 8.00±3.16. P<0.05, P< 0.01).② After acupuncture stimulus or thermal stimulus, 5-HT was released by MCs in the acupoint area, which aggregated around the blood vessels,and the number of 5-HT in the acupoint area significantly increased when compared with that in blank group(the blank group 3.00 ±1.28 vs. the acupuncture stimulus group 10.02 ±3.21;the blank group3.00 ±1.28 vs. the thermal stimulus group 14.00 ±3.94, both P< 0.01).③ Compared with blank group,both acupuncture stimulus and thermal stimulus could reduce the 5-HT content in serum(the blank group 0.72 ±0.2372 vs. acupuncture stimulus group 0.43 ±0.21: the blank group 0.72 ±0.24 vs. thermal stimulus group 0.32 ±0.18, both P<0.01), and the effect in thermal stimulus group was slightly superior to that in acupuncture stimulus group(P<0.05).Conclusion: Both acupuncture stimulus and thermal stimulus can cause the aggregation and degranulation of MCs and high expression of 5-HT in the acupoint area. The effect of thermal stimulus was superior to that of acupuncture stimulus in degranulation.
文摘5-HT1A基因编码5-羟色胺(血清素)的G蛋白偶联受体(属于5-羟色胺受体亚家族)。C-1019G多态位点是5-HT1A基因启动子区一个重要的功能性多态位点,人群中存在3种基因型即CC、CG、GG,它与个体的恋爱关系及抑郁症、焦虑症等精神疾病密切相关。本文对5-HT1A基因C-1019G多态位点的相关机制、检测方法、研究进展等进行综述。The 5-HT1A gene encodes a G protein-coupled receptor for serotonin, which belongs to the 5-hydroxytryptamine receptor subfamily. C-1019G polymorphism is an important functional poly-morphism in the promoter region of 5-HT1A gene. There are three genotypes (CC, CG and GG) in the population, which are closely related to individual’s romantic relationship and mental disorders such as depression and anxiety. In this paper, the mechanism, detection methods and research progress of C-1019G polymorphism of 5-HT1A gene were reviewed.
基金National natural science foundation of China(No.81904294)Key natural science research project of Anhui(No.KJ2018A0297)。
文摘Objective:To investigate the effects of electricity on the expression of procalcitonin(CGRP)and serotonin(5-HT)in serum and colon in rats with constipation-type irritable bowel syndrome(IBS-C).Methods:50 rats were randomly divided into three groups:normal control group,model group,electroacupuncture group with four single points(tianshu electroacupuncture group,Colonic acupuncture group,Quchi electroacupuncture group,Shangjuxu acupoint group,and bilateral acupoint alternating between left and right),and non-meridian non-acupoint group(non-meridian non-acupoint group on both hip of electroacupuncture rats).The IBS-C model was replicated in the other three groups,except the normal control group,using the cold-fear-starvation synthesis method.After successful replication of the model,the four electroacupuncture single-point group and the non-acupoint group began electroacupuncture treatment once a day for 3 consecutive days.The visceral sensitivity and the expression of CGRP and 5-HT in serum and colon were observed.Results:Compared with the normal control group,the minimum volume threshold of abdominal retractile reflex(AWR)in the model group decreased(P<0.05),and the expressions of 5-HT and CGRP in serum and colon increased(P<0.05).Compared with the model group,AWR minimum volume threshold was increased in the electroacupuncture group with four single acupuncture points(P<0.05),the expression of 5-ht and CGRP in serum and colon was significantly decreased(P<0.05),and there was no significant change in each indicator in the non-acupuncture point group(P>0.05).Conclusion:Electroacupuncture combined with four single points can effectively improve the visceral sensitivity of IBS-C rats and regulate the levels of 5-HT and CGRP in serum and colon of rats,thus providing experimental basis for the clinical treatment of IBS-C.
