BACKGROUND Rectal carcinoma(RC),one of the most common malignancies globally,presents an increasing incidence and mortality year by year,especially among young people,which seriously affects the prognosis and quality ...BACKGROUND Rectal carcinoma(RC),one of the most common malignancies globally,presents an increasing incidence and mortality year by year,especially among young people,which seriously affects the prognosis and quality of life of patients.At present,dynamic contrast-enhanced magnetic resonance imaging(DCE-MRI)parameters and serum carbohydrate antigen 19-9(CA19-9)and CA125 Levels have been used in clinical practice to evaluate the T stage and differentiation of RC.However,the accuracy of these evaluation modalities still needs further research.This study explores the application and value of these methods in evaluating the T stage and differentiation degree of RC.AIM To analyze the diagnostic performance of DCE-MRI parameters combined with serum tumor markers(TMs)in assessing pathological processes and prognosis of RC patients.METHODS A retrospective analysis was performed on 104 RC patients treated at Yantai Yuhuangding Hospital from May 2018 to January 2022.Patients were categorized into stages T1,T2,T3,and T4,depending on their T stage and differentiation degree.In addition,they were assigned to low(L group)and moderate-high differentiation(M+H group)groups based on their differentiation degree.The levels of DCE-MRI parameters and serum CA19-9 and CA125 in different groups of patients were compared.In addition,the value of DCE-MRI parameters[volume transfer constant(Ktrans),rate constant(Kep),and extravascular extracellular volume fraction(Ve)in assessing the differentiation and T staging of RC patients was discussed.Furthermore,the usefulness of DCE-MRI parameters combined with serum CA19-9 and CA125 Levels in the evaluation of RC differentiation and T staging was analyzed.RESULTS Ktrans,Ve,CA19-9 and CA125 were higher in the high-stage group and L group than in the low-stage group and M+H Group,respectively(P<0.05).The areas under the curve(AUCs)of the Ktran and Ve parameters were 0.638 and 0.694 in the diagnosis of high and low stages,respectively,and 0.672 and 0.725 in diagnosing moderate-high and low differentiation,respectively.The AUC of DCE-MRI parameters(Ktrans+Ve)in the diagnosis of high and low stages was 0.742,and the AUC in diagnosing moderate-high and low differentiation was 0.769.The AUCs of CA19-9 and CA-125 were 0.773 and 0.802 in the diagnosis of high and low stages,respectively,and 0.834 and 0.796 in diagnosing moderate-high and low differentiation,respectively.Then,we combined DCE-MRI(Ktrans+Ve)parameters with CA19-9 and CA-125 and found that the AUC of DCE-MRI parameters plus serum TMs was 0.836 in the diagnosis of high and low stages and 0.946 in the diagnosis of moderate-high and low differentiation.According to the Delong test,the AUC of DCE-MRI parameters plus serum TMs increased significantly compared with serum TMs alone in the diagnosis of T stage and differentiation degree(P<0.001).CONCLUSION The levels of the DCE-MRI parameters Ktrans and Ve and the serum TMs CA19-9 and CA125 all increase with increasing T stage and decreasing differentiation degree of RC and can be used as indices to evaluate the differentiation degree of RC in clinical practice.Moreover,the combined evaluation of the above indices has a better effect and more obvious clinical value,providing important guiding importance for clinical condition judgment and treatment selection.展开更多
BACKGROUND Gastric cancer is a kind of malignant tumor which is prevalent all over the world.Although some progress has been made in the treatment of gastric cancer,its prognosis is still not optimistic,so it is of gr...BACKGROUND Gastric cancer is a kind of malignant tumor which is prevalent all over the world.Although some progress has been made in the treatment of gastric cancer,its prognosis is still not optimistic,so it is of great significance to find reliable prog-nostic indicators to guide the treatment and management of patients with gastric cancer.AIM To explore the relationship between serum levels of five biomarkers[carcinoem-bryonic antigen(CEA),carbohydrate antigen(CA)19-9,CA72-4,CA24-2,and ferritin]and prognosis in patients with gastric cancer.METHODS This study included 200 patients with gastric adenocarcinoma,and conducted an in-depth analysis of their baseline characteristics,relationship between tumor markers and staging,and prognosis.The study found that CA19-9 has a signi-ficant correlation with tumor stage,the average levels of CA24-2,CEA,CA72-4 and ferritin were slightly increased disregarding the stage of tumor.Survival analysis showed that increases in CEA,CA19-9,CA24-2,and ferritin were all associated with shortened overall survival of patients.Further multivariate ana-lysis revealed that elevated serum CA72-4 levels were an inde-pendent adverse prognostic factor.RESULTS This study reveals that there is a significant correlation between the expression levels of serum tumor markers CEA,CA19-9,CA72-4,CA24-2 and ferritin in patients with gastric cancer and prognosis,and can be used as important indicators for prognostic evaluation of gastric cancer.In particular,markers that appear abnormally elevated initially may help identify gastric cancer patients with poor prognosis.CONCLUSION Serum CEA and CA19-9 play an important role in the prognosis assessment of gastric cancer,and are effective tools to guide clinical practice and optimize individualized treatment strategies for gastric cancer patients.展开更多
目的探讨在炎性激活条件下的椎间盘退变(IDD)过程中miR-335-3p靶向调控趋化因子配体(CCL)5的机制及可能抑制IDD进展的方法。方法建立人髓核(NP)细胞的原代培养,肿瘤坏死因子(TNF)-α诱导NP细胞,Trizol法获取NP细胞总RNA,随后对mRNA及mi...目的探讨在炎性激活条件下的椎间盘退变(IDD)过程中miR-335-3p靶向调控趋化因子配体(CCL)5的机制及可能抑制IDD进展的方法。方法建立人髓核(NP)细胞的原代培养,肿瘤坏死因子(TNF)-α诱导NP细胞,Trizol法获取NP细胞总RNA,随后对mRNA及miRNA进行高通量测序分析。对miR-335-3p靶向结合mRNA进行预测,结合STRING富集分析miRNA靶标基因,确定TNF-α影响NP细胞的关键基因。应用The Human Protein Atlas(https://www.proteinatlas.org/)分析CCL5及其下游调控蛋白受体趋化因子受体(CCR)5基因在免疫相关细胞中的表达水平。应用Targetscan预测miR-335-3p与CCL53′UTR序列的靶向结合位点。结合promega的双荧光素酶报告系统使用turner仪器进行测量。将人NP细胞分成:Control组(NP细胞)、TNF-α组、mimic-NC组、miR-335-3p mimic组。使用Lipofectamine RNAiMAX将miR-335-3p mimic或miR-NC寡核苷酸序列转染入人NP细胞。采用实时荧光定量聚合酶链反应(qRT-PCR)方法检测miR-335-3p的表达情况。Western印迹实验检测NP细胞外基质相关蛋白的表达水平。结果与Control组比较,TNF-α组miR-335-3p基因表达水平显著下调(P<0.01),CCL5基因表达水平显著上调(P<0.01);miR-335-3p mimic组miR-335-3p表达水平较mimic-NC组显著上调(P<0.01),CCL5表达水平显著较mimic-NC组下调(P<0.01)。与Control组比较,TNF-α组生长分化因子(GDF)5和性别决定区Y框蛋白(SOX)9蛋白显著下调,而基质金属蛋白酶(MMP)2蛋白表达水平显著上调(P<0.01);与mimic-NC组比较,miR-335-3p mimic组MMP2蛋白表达水平显著下调,而GDF5和SOX9蛋白显著上调(P<0.01)。结论miR-335-3p在TNF-α诱导的炎症条件下可特异性下调CCL5,从而逆转TNF-α诱导的软骨生成因子SOX9的异常表达。展开更多
Purpose: To study the mechanism of early acupuncture treatment in resisting inflammatory injury in cerebral ischemia reperfusion (IR) rats. Methods: A total of 120 Wistar rats were randomly divided into normal control...Purpose: To study the mechanism of early acupuncture treatment in resisting inflammatory injury in cerebral ischemia reperfusion (IR) rats. Methods: A total of 120 Wistar rats were randomly divided into normal control,sham operation, model and EA groups, and each of them were further divided into IR 12 hr, IR 24 hr and IR 48 hr subgroups, with 10 cases being in each subgroup. Cerebral ischemia was produced by occlusion of the middle cerebral artery for 0.5 hr. Shuigou (GV 26) and Baihui (GV 20) were punctured and stimulated electrically with an EA therapeutic apparatus. Serum IL 1β and tumor necrosis factor (TNF) α contents were assayed by using radioimmunoassay. Results: Results showed that serum IL 1β and TNF α contents of model group were significantly higher than those of normal control and sham operation groups at IR 12 hr, 24 hr and 48 hr (P<0.05), meaning that following IR, the production and release of these two cytokines in the blood increased obviously; comparison between EA and model groups displayed that serum IL 1β and TNF α levels of EA group were strikingly lower than those of model group (P<0.01), suggesting that EA stimulation can suppress IR induced increase of production and release of these two cytokines. Conclusion: EA can suppress cerebral IR induced increase of serum IL 1β and TNF α, which may contribute to the effect of acupuncture in resisting cerebral IR injury in the treatment of stroke.展开更多
BACKGROUND Hepatic metastases are common and difficult to treat after colorectal cancer(CRC)surgery.The predictive value of carcinoembryonic antigen(CEA),cancer antigen(CA)125 and CA19-9 combined tests for liver metas...BACKGROUND Hepatic metastases are common and difficult to treat after colorectal cancer(CRC)surgery.The predictive value of carcinoembryonic antigen(CEA),cancer antigen(CA)125 and CA19-9 combined tests for liver metastasis is unclear.AIM To evaluate predictive value of combined tests for CEA,CA125,and CA19-9 levels in patients with liver metastases of CRC.METHODS The retrospective study included patients with CRC alone(50 cases)and patients with CRC combined with liver metastases(50 cases)who were hospitalized between January 2021 and January 2023.Serum CEA,CA125 and CA19-9 levels were compared between the two groups,and binary logistic regression was used to analyze the predictive value of the combination of these tumor markers in liver metastasis.In addition,we performed receiver operating characteristic(ROC)curve analysis to assess its diagnostic accuracy.RESULTS The results showed that the serum CEA,CA125 and CA19-9 levels in the CRC with liver metastasis group were significantly higher than those in the CRC alone group.Specifically,the average serum CEA level in the CRC with liver metastasis group was 162.03±810.01 ng/mL,while that in the CRC alone group was 5.71±9.76 ng/mL;the average serum CA125 levels were 43.47±83.52 U/mL respectively.and 13.5±19.68 U/mL;the average serum CA19-9 levels were 184.46±473.13 U/mL and 26.55±43.96 U/mL respectively.In addition,binary logistic regression analysis showed that CA125 was significant in predicting CRC liver metastasis(P<0.05).ROC curve analysis results showed that the areas under the ROC curves of CEA,CA125 and CA19-9 were 0.607,0.692 and 0.586.CONCLUSION These results suggest that combined detection of these tumor markers may help early detection and intervention of CRC liver metastasis,thereby improving patient prognosis.展开更多
文摘BACKGROUND Rectal carcinoma(RC),one of the most common malignancies globally,presents an increasing incidence and mortality year by year,especially among young people,which seriously affects the prognosis and quality of life of patients.At present,dynamic contrast-enhanced magnetic resonance imaging(DCE-MRI)parameters and serum carbohydrate antigen 19-9(CA19-9)and CA125 Levels have been used in clinical practice to evaluate the T stage and differentiation of RC.However,the accuracy of these evaluation modalities still needs further research.This study explores the application and value of these methods in evaluating the T stage and differentiation degree of RC.AIM To analyze the diagnostic performance of DCE-MRI parameters combined with serum tumor markers(TMs)in assessing pathological processes and prognosis of RC patients.METHODS A retrospective analysis was performed on 104 RC patients treated at Yantai Yuhuangding Hospital from May 2018 to January 2022.Patients were categorized into stages T1,T2,T3,and T4,depending on their T stage and differentiation degree.In addition,they were assigned to low(L group)and moderate-high differentiation(M+H group)groups based on their differentiation degree.The levels of DCE-MRI parameters and serum CA19-9 and CA125 in different groups of patients were compared.In addition,the value of DCE-MRI parameters[volume transfer constant(Ktrans),rate constant(Kep),and extravascular extracellular volume fraction(Ve)in assessing the differentiation and T staging of RC patients was discussed.Furthermore,the usefulness of DCE-MRI parameters combined with serum CA19-9 and CA125 Levels in the evaluation of RC differentiation and T staging was analyzed.RESULTS Ktrans,Ve,CA19-9 and CA125 were higher in the high-stage group and L group than in the low-stage group and M+H Group,respectively(P<0.05).The areas under the curve(AUCs)of the Ktran and Ve parameters were 0.638 and 0.694 in the diagnosis of high and low stages,respectively,and 0.672 and 0.725 in diagnosing moderate-high and low differentiation,respectively.The AUC of DCE-MRI parameters(Ktrans+Ve)in the diagnosis of high and low stages was 0.742,and the AUC in diagnosing moderate-high and low differentiation was 0.769.The AUCs of CA19-9 and CA-125 were 0.773 and 0.802 in the diagnosis of high and low stages,respectively,and 0.834 and 0.796 in diagnosing moderate-high and low differentiation,respectively.Then,we combined DCE-MRI(Ktrans+Ve)parameters with CA19-9 and CA-125 and found that the AUC of DCE-MRI parameters plus serum TMs was 0.836 in the diagnosis of high and low stages and 0.946 in the diagnosis of moderate-high and low differentiation.According to the Delong test,the AUC of DCE-MRI parameters plus serum TMs increased significantly compared with serum TMs alone in the diagnosis of T stage and differentiation degree(P<0.001).CONCLUSION The levels of the DCE-MRI parameters Ktrans and Ve and the serum TMs CA19-9 and CA125 all increase with increasing T stage and decreasing differentiation degree of RC and can be used as indices to evaluate the differentiation degree of RC in clinical practice.Moreover,the combined evaluation of the above indices has a better effect and more obvious clinical value,providing important guiding importance for clinical condition judgment and treatment selection.
文摘BACKGROUND Gastric cancer is a kind of malignant tumor which is prevalent all over the world.Although some progress has been made in the treatment of gastric cancer,its prognosis is still not optimistic,so it is of great significance to find reliable prog-nostic indicators to guide the treatment and management of patients with gastric cancer.AIM To explore the relationship between serum levels of five biomarkers[carcinoem-bryonic antigen(CEA),carbohydrate antigen(CA)19-9,CA72-4,CA24-2,and ferritin]and prognosis in patients with gastric cancer.METHODS This study included 200 patients with gastric adenocarcinoma,and conducted an in-depth analysis of their baseline characteristics,relationship between tumor markers and staging,and prognosis.The study found that CA19-9 has a signi-ficant correlation with tumor stage,the average levels of CA24-2,CEA,CA72-4 and ferritin were slightly increased disregarding the stage of tumor.Survival analysis showed that increases in CEA,CA19-9,CA24-2,and ferritin were all associated with shortened overall survival of patients.Further multivariate ana-lysis revealed that elevated serum CA72-4 levels were an inde-pendent adverse prognostic factor.RESULTS This study reveals that there is a significant correlation between the expression levels of serum tumor markers CEA,CA19-9,CA72-4,CA24-2 and ferritin in patients with gastric cancer and prognosis,and can be used as important indicators for prognostic evaluation of gastric cancer.In particular,markers that appear abnormally elevated initially may help identify gastric cancer patients with poor prognosis.CONCLUSION Serum CEA and CA19-9 play an important role in the prognosis assessment of gastric cancer,and are effective tools to guide clinical practice and optimize individualized treatment strategies for gastric cancer patients.
文摘目的探讨在炎性激活条件下的椎间盘退变(IDD)过程中miR-335-3p靶向调控趋化因子配体(CCL)5的机制及可能抑制IDD进展的方法。方法建立人髓核(NP)细胞的原代培养,肿瘤坏死因子(TNF)-α诱导NP细胞,Trizol法获取NP细胞总RNA,随后对mRNA及miRNA进行高通量测序分析。对miR-335-3p靶向结合mRNA进行预测,结合STRING富集分析miRNA靶标基因,确定TNF-α影响NP细胞的关键基因。应用The Human Protein Atlas(https://www.proteinatlas.org/)分析CCL5及其下游调控蛋白受体趋化因子受体(CCR)5基因在免疫相关细胞中的表达水平。应用Targetscan预测miR-335-3p与CCL53′UTR序列的靶向结合位点。结合promega的双荧光素酶报告系统使用turner仪器进行测量。将人NP细胞分成:Control组(NP细胞)、TNF-α组、mimic-NC组、miR-335-3p mimic组。使用Lipofectamine RNAiMAX将miR-335-3p mimic或miR-NC寡核苷酸序列转染入人NP细胞。采用实时荧光定量聚合酶链反应(qRT-PCR)方法检测miR-335-3p的表达情况。Western印迹实验检测NP细胞外基质相关蛋白的表达水平。结果与Control组比较,TNF-α组miR-335-3p基因表达水平显著下调(P<0.01),CCL5基因表达水平显著上调(P<0.01);miR-335-3p mimic组miR-335-3p表达水平较mimic-NC组显著上调(P<0.01),CCL5表达水平显著较mimic-NC组下调(P<0.01)。与Control组比较,TNF-α组生长分化因子(GDF)5和性别决定区Y框蛋白(SOX)9蛋白显著下调,而基质金属蛋白酶(MMP)2蛋白表达水平显著上调(P<0.01);与mimic-NC组比较,miR-335-3p mimic组MMP2蛋白表达水平显著下调,而GDF5和SOX9蛋白显著上调(P<0.01)。结论miR-335-3p在TNF-α诱导的炎症条件下可特异性下调CCL5,从而逆转TNF-α诱导的软骨生成因子SOX9的异常表达。
文摘Purpose: To study the mechanism of early acupuncture treatment in resisting inflammatory injury in cerebral ischemia reperfusion (IR) rats. Methods: A total of 120 Wistar rats were randomly divided into normal control,sham operation, model and EA groups, and each of them were further divided into IR 12 hr, IR 24 hr and IR 48 hr subgroups, with 10 cases being in each subgroup. Cerebral ischemia was produced by occlusion of the middle cerebral artery for 0.5 hr. Shuigou (GV 26) and Baihui (GV 20) were punctured and stimulated electrically with an EA therapeutic apparatus. Serum IL 1β and tumor necrosis factor (TNF) α contents were assayed by using radioimmunoassay. Results: Results showed that serum IL 1β and TNF α contents of model group were significantly higher than those of normal control and sham operation groups at IR 12 hr, 24 hr and 48 hr (P<0.05), meaning that following IR, the production and release of these two cytokines in the blood increased obviously; comparison between EA and model groups displayed that serum IL 1β and TNF α levels of EA group were strikingly lower than those of model group (P<0.01), suggesting that EA stimulation can suppress IR induced increase of production and release of these two cytokines. Conclusion: EA can suppress cerebral IR induced increase of serum IL 1β and TNF α, which may contribute to the effect of acupuncture in resisting cerebral IR injury in the treatment of stroke.
文摘BACKGROUND Hepatic metastases are common and difficult to treat after colorectal cancer(CRC)surgery.The predictive value of carcinoembryonic antigen(CEA),cancer antigen(CA)125 and CA19-9 combined tests for liver metastasis is unclear.AIM To evaluate predictive value of combined tests for CEA,CA125,and CA19-9 levels in patients with liver metastases of CRC.METHODS The retrospective study included patients with CRC alone(50 cases)and patients with CRC combined with liver metastases(50 cases)who were hospitalized between January 2021 and January 2023.Serum CEA,CA125 and CA19-9 levels were compared between the two groups,and binary logistic regression was used to analyze the predictive value of the combination of these tumor markers in liver metastasis.In addition,we performed receiver operating characteristic(ROC)curve analysis to assess its diagnostic accuracy.RESULTS The results showed that the serum CEA,CA125 and CA19-9 levels in the CRC with liver metastasis group were significantly higher than those in the CRC alone group.Specifically,the average serum CEA level in the CRC with liver metastasis group was 162.03±810.01 ng/mL,while that in the CRC alone group was 5.71±9.76 ng/mL;the average serum CA125 levels were 43.47±83.52 U/mL respectively.and 13.5±19.68 U/mL;the average serum CA19-9 levels were 184.46±473.13 U/mL and 26.55±43.96 U/mL respectively.In addition,binary logistic regression analysis showed that CA125 was significant in predicting CRC liver metastasis(P<0.05).ROC curve analysis results showed that the areas under the ROC curves of CEA,CA125 and CA19-9 were 0.607,0.692 and 0.586.CONCLUSION These results suggest that combined detection of these tumor markers may help early detection and intervention of CRC liver metastasis,thereby improving patient prognosis.
