目的研究呼出气一氧化氮(FeNO)、血清免疫球蛋白E(IgE)和基质金属蛋白酶9(MMP9)的水平与儿童哮喘急性发作之间的关系,为儿童哮喘的预防及治疗提供依据。方法选取沈阳市妇婴医院于2020年11月至2022年11月收治的98例支气管哮喘急性发作期...目的研究呼出气一氧化氮(FeNO)、血清免疫球蛋白E(IgE)和基质金属蛋白酶9(MMP9)的水平与儿童哮喘急性发作之间的关系,为儿童哮喘的预防及治疗提供依据。方法选取沈阳市妇婴医院于2020年11月至2022年11月收治的98例支气管哮喘急性发作期儿童作为急性组,按照病情程度分成轻度组(n=32)、中度组(n=38)和重度组(n=28),按照2∶1的比例选出49例同期在门诊治疗的处于支气管哮喘缓解期的儿童作为缓解组,随机选取健康体检儿童49例作为健康对照组,分别对他们进行FeNO、MMP9和血清IgE及肺功能[用力肺活量(FVC)、1秒用力呼气量(FEV_(1))、FEV_(1)/FVC%、最大呼气流量(PEF)]检测。应用Pearson相关分析探讨哮喘急性发作期FeNO、MMP9及血清IgE和肺功能之间的联系,并对三者在支气管哮喘急性发作中的预测价值进行分析。结果急性组、缓解组和对照组的年龄、性别、体重指数和病程的比较,差异无统计学意义(P>0.05)。急性组FeNO、MMP9、血清IgE分别为(59.95±12.65)ppb、(4.87±1.44)pg/ml、(330.63±74.88)IU/ml,缓解组分别为(25.23±8.23)ppb、(1.21±0.02)pg/ml、(152.23±32.12)IU/ml,均高于对照组的(12.43±4.09)ppb、(0.53±0.24)pg/ml、(126.34±57.33)IU/ml,差异具有统计学意义(P<0.05)。急性期和缓解期FVC、FEV1、FEV1/FVC%、PEF均低于对照组,差异具有统计学意义(P<0.05)。支气管哮喘急性发作中度组FeNO、MMP9、血清IgE水平分别为(49.23±6.23)ppb、(1.21±0.02)pg/ml、(282.61±59.83)IU/ml,重度组分别为(67.43±10.09)ppb、(0.53±0.24)pg/ml、(356.49±70.82)IU/ml,均高于轻度组的(34.62±10.65)ppb、(4.87±1.44)pg/ml,(189.21±14.33)IU/ml,差异具有统计学意义(P<0.05)。在轻度组中FeNO、MMP9和血清IgE水平均较低,而在中度组中这些指标均较高,其中FVC、FEV_(1)、FEV_(1)/FVC%和PEF均较低,差异具有统计学意义(P<0.05)。FeNO以及MMP9与血清IgE水平呈正相关(P<0.05),FeNO、MMP9以及血清IgE水平与FVC、FEV_(1)、FEV_(1)/FVC%、PEF均呈负相关(P<0.05)。MMP9在支气管哮喘的诊断中表现出了显著的优势,当达到最大约登指数时,对应的截断值为1.17,曲线下面积(Area under curve,AUC)为0.83,敏感度和特异性也分别达到了90.13%和86.5%。结论支气管哮喘急性发作的儿童血清中的FeNO、MMP9以及血清IgE水平显著增高,随肺部功能恶化程度加重而上升,可能与支气管哮喘急性发作患儿肺功能损害程度有关。展开更多
Objective To investigate the serum total IgE （tlgE） and specific IgE （slgE） to common allergens among allergic patients in Guangzhou, China. Methods 7 085 patients were examined for tlgE and slgE to 15 allergens, ...Objective To investigate the serum total IgE （tlgE） and specific IgE （slgE） to common allergens among allergic patients in Guangzhou, China. Methods 7 085 patients were examined for tlgE and slgE to 15 allergens, based on the protocols of reversed enzyme allergosorbent test and the sandwich enzyme-linked immunosorbent assay. Results 3 758 （53.04%） patients tested positive for tlgE, and 4 640 （65.49%） for slgE. Der pteronyssinus, Derfarinae, eggs, and cow＇s milk were the most common allergens leading to higher positive rates of slgE responses. Several peaks of sensitization were： Der pteronyssinus, Derfarinae, and Blomia tropicalis at age 10-12; cow＇s milk at age below 3; eggs at age 4-6. The mean level and positive rate of tlgE tended to increase in subjects sensitized to more allergens. Sensitization to Der pteronyssinus （OR, 1.6; P〈O.05）, Der farinae （OR, 1.5; P〈O.05）, Blomia tropicalis （OR, 1.4; P〈O.05）, Blattella germanica （OR, 1.5; P〈O.05）, cow＇s milk （OR, 1.3; P〈O.05）, and soy beans （OR, 2.0; P〈O.05） were independently correlated with allergy-related conditions in preliminary diagnosis. Conclusion The major allergens in Guangzhou include Derpteronyssinus, Derfarinae, cow＇s milk, and eggs. Sensitization to these allergens appears to be predictors of allergy-related disorder.展开更多
Summary: In order to study the relationship between serum specific IgE (sIgE) and allergen skin test, allergen skin tests and detections of sIgE in 220 allergic patients of Wuhan area were analyzed. The coherent rate ...Summary: In order to study the relationship between serum specific IgE (sIgE) and allergen skin test, allergen skin tests and detections of sIgE in 220 allergic patients of Wuhan area were analyzed. The coherent rate of the two methods was beyond 70 % (P<0.01). It was concluded that the in vitro and in vivo detection methods of allergens have a high coherence and can be used as the effective ways to diagnose the allergic diseases in clinical practice.展开更多
Objective:To investigate the levels of serum vitamin D and IgE in infants with different allergic risks and to analyze their correlations.Methods:In the present study,the participants were selected and recruited from ...Objective:To investigate the levels of serum vitamin D and IgE in infants with different allergic risks and to analyze their correlations.Methods:In the present study,the participants were selected and recruited from the infant ward of our hospital from February 2012 to December 2012.A total of 120 patients were selected and recruited.Among these patients,64 patients with symptoms of allergic diseases were assigned to the experimental group.The remaining 56 patients who had respiratory infections,but without other symptoms of allergic diseases,were assigned to the control group.The serum 25-hydroxyvitamin D3 levels were measured using liquid chromatography tandem mass spectrometry and the IgE levels were measured using radioimmunoassay in both experimental and control groups.Subsequently,the serum 25-hydroxyvitamin D3 levels and IgE levels were compared between the experimental group and control group.Results:The following findings were obtained through data comparison.The serum 25-hydroxyvitamin D3 level in the experimental group was 18.19±7.84 ng/mL,and the IgE level was 551.51±705.88 IU/mL.The serum 25-hydroxyvitamin D3 level in the control group was 21.95±7.834 ng/mL,IgE level was 49.39±32.46 IU/mL.The data comparison indicates that the serum 25-hydroxyvitamin D3 level in the control group was higher than the experimental group,and the IgE level in the control group was lower than the experimental group(P<0.05).Conclusion:The level of vitamin D3 in patients with allergic symptoms is relatively low.This may be one of the causes leading to allergies.At the same time,IgE is also one of the causes contributing to allergies.An elevation of the IgE level is very likely to cause the allergic,and there is a correlation between serum vitamin D and IgE levels.At the same time,research has found that proper supplementation of vitamin D3 can help prevent allergic diseases,and can be considered in a new and highquality program for the prevention of allergic diseases.展开更多
文摘目的研究呼出气一氧化氮(FeNO)、血清免疫球蛋白E(IgE)和基质金属蛋白酶9(MMP9)的水平与儿童哮喘急性发作之间的关系,为儿童哮喘的预防及治疗提供依据。方法选取沈阳市妇婴医院于2020年11月至2022年11月收治的98例支气管哮喘急性发作期儿童作为急性组,按照病情程度分成轻度组(n=32)、中度组(n=38)和重度组(n=28),按照2∶1的比例选出49例同期在门诊治疗的处于支气管哮喘缓解期的儿童作为缓解组,随机选取健康体检儿童49例作为健康对照组,分别对他们进行FeNO、MMP9和血清IgE及肺功能[用力肺活量(FVC)、1秒用力呼气量(FEV_(1))、FEV_(1)/FVC%、最大呼气流量(PEF)]检测。应用Pearson相关分析探讨哮喘急性发作期FeNO、MMP9及血清IgE和肺功能之间的联系,并对三者在支气管哮喘急性发作中的预测价值进行分析。结果急性组、缓解组和对照组的年龄、性别、体重指数和病程的比较,差异无统计学意义(P>0.05)。急性组FeNO、MMP9、血清IgE分别为(59.95±12.65)ppb、(4.87±1.44)pg/ml、(330.63±74.88)IU/ml,缓解组分别为(25.23±8.23)ppb、(1.21±0.02)pg/ml、(152.23±32.12)IU/ml,均高于对照组的(12.43±4.09)ppb、(0.53±0.24)pg/ml、(126.34±57.33)IU/ml,差异具有统计学意义(P<0.05)。急性期和缓解期FVC、FEV1、FEV1/FVC%、PEF均低于对照组,差异具有统计学意义(P<0.05)。支气管哮喘急性发作中度组FeNO、MMP9、血清IgE水平分别为(49.23±6.23)ppb、(1.21±0.02)pg/ml、(282.61±59.83)IU/ml,重度组分别为(67.43±10.09)ppb、(0.53±0.24)pg/ml、(356.49±70.82)IU/ml,均高于轻度组的(34.62±10.65)ppb、(4.87±1.44)pg/ml,(189.21±14.33)IU/ml,差异具有统计学意义(P<0.05)。在轻度组中FeNO、MMP9和血清IgE水平均较低,而在中度组中这些指标均较高,其中FVC、FEV_(1)、FEV_(1)/FVC%和PEF均较低,差异具有统计学意义(P<0.05)。FeNO以及MMP9与血清IgE水平呈正相关(P<0.05),FeNO、MMP9以及血清IgE水平与FVC、FEV_(1)、FEV_(1)/FVC%、PEF均呈负相关(P<0.05)。MMP9在支气管哮喘的诊断中表现出了显著的优势,当达到最大约登指数时,对应的截断值为1.17,曲线下面积(Area under curve,AUC)为0.83,敏感度和特异性也分别达到了90.13%和86.5%。结论支气管哮喘急性发作的儿童血清中的FeNO、MMP9以及血清IgE水平显著增高,随肺部功能恶化程度加重而上升,可能与支气管哮喘急性发作患儿肺功能损害程度有关。
文摘探讨俞募配穴针法联合穴位埋针法治疗风痰闭阻型支气管哮喘的疗效及对血清免疫球蛋白E(Immunoglobin E,IgE)、可溶性二聚体细胞因子(Interferon-γ,IFN-γ)、肺表面活性蛋白(Pulmonary surfactant protein,SP-A)水平影响。方法选取医院收治的风痰闭阻型支气管哮喘患者108例,采用区组随机化原则分为两组各54例。对照组给予穴位埋针法,治疗组在对照组基础上给予俞募配穴针法,两组患者数据观察:对比治疗后临床疗效、哮喘控制测试(Asthma control test,ACT)量表评分、第一秒最大呼气容积(Forced expiratory volume in one second,FEV1)、呼气峰值流速(Peak expiratory,PEF)、用力肺活量(Forced vital capacity,FVC)、临床症状评分及IgE、IFN-γ、SP-A水平、临床治疗安全性及患者满意度。结果经过治疗后,治疗组临床疗效显著较高(P<0.05);两组治疗前ACT评分差异无统计学意义(P>0.05);治疗后两组ACT评分明显升高(P<0.05);且治疗组升高较明显(P<0.05);治疗后两组FEV1、PEF、FVC值明显升高(P<0.05);且治疗组升高较明显(P<0.05);两组治疗前临床症状评分差异无统计学意义(P>0.05);两组治疗后咳嗽、呼吸困难、喘息等症状评分显著降低(P<0.05);且治疗组降低较明显(P<0.05);两组治疗前血清IgE、IFN-γ、SP-A水平无差异(P>0.05);两组治疗后血清IgE显著降低,血清IFN-γ、SP-A水平显著升高(P<0.05);且治疗组改善较明显(P<0.05);治疗组未见治疗不良反应,对照组患者出现1例不良反应,症状为恶心,P>0.05;治疗组治疗满意率高达98.15%(53/54),显著高于对照组的83.33%(45/54),P<0.05。结论采用俞募配穴针法联合穴位埋针法治疗风痰闭阻型支气管哮喘具有较好的治疗效果,能够改善血清IgE、IFN-γ及SP-A水平,且本次治疗安全可靠,患者十分认可,值得在临床上推广应用。
基金supported by Guangdong Industry Technology and Development Fund(No.2011B061200040)Science Foundation of Guangzhou Medical Univesity(Project No.2013A06)Guangdong Natural Science Foundation(No.S2013010014728)
文摘Objective To investigate the serum total IgE （tlgE） and specific IgE （slgE） to common allergens among allergic patients in Guangzhou, China. Methods 7 085 patients were examined for tlgE and slgE to 15 allergens, based on the protocols of reversed enzyme allergosorbent test and the sandwich enzyme-linked immunosorbent assay. Results 3 758 （53.04%） patients tested positive for tlgE, and 4 640 （65.49%） for slgE. Der pteronyssinus, Derfarinae, eggs, and cow＇s milk were the most common allergens leading to higher positive rates of slgE responses. Several peaks of sensitization were： Der pteronyssinus, Derfarinae, and Blomia tropicalis at age 10-12; cow＇s milk at age below 3; eggs at age 4-6. The mean level and positive rate of tlgE tended to increase in subjects sensitized to more allergens. Sensitization to Der pteronyssinus （OR, 1.6; P〈O.05）, Der farinae （OR, 1.5; P〈O.05）, Blomia tropicalis （OR, 1.4; P〈O.05）, Blattella germanica （OR, 1.5; P〈O.05）, cow＇s milk （OR, 1.3; P〈O.05）, and soy beans （OR, 2.0; P〈O.05） were independently correlated with allergy-related conditions in preliminary diagnosis. Conclusion The major allergens in Guangzhou include Derpteronyssinus, Derfarinae, cow＇s milk, and eggs. Sensitization to these allergens appears to be predictors of allergy-related disorder.
文摘Summary: In order to study the relationship between serum specific IgE (sIgE) and allergen skin test, allergen skin tests and detections of sIgE in 220 allergic patients of Wuhan area were analyzed. The coherent rate of the two methods was beyond 70 % (P<0.01). It was concluded that the in vitro and in vivo detection methods of allergens have a high coherence and can be used as the effective ways to diagnose the allergic diseases in clinical practice.
文摘Objective:To investigate the levels of serum vitamin D and IgE in infants with different allergic risks and to analyze their correlations.Methods:In the present study,the participants were selected and recruited from the infant ward of our hospital from February 2012 to December 2012.A total of 120 patients were selected and recruited.Among these patients,64 patients with symptoms of allergic diseases were assigned to the experimental group.The remaining 56 patients who had respiratory infections,but without other symptoms of allergic diseases,were assigned to the control group.The serum 25-hydroxyvitamin D3 levels were measured using liquid chromatography tandem mass spectrometry and the IgE levels were measured using radioimmunoassay in both experimental and control groups.Subsequently,the serum 25-hydroxyvitamin D3 levels and IgE levels were compared between the experimental group and control group.Results:The following findings were obtained through data comparison.The serum 25-hydroxyvitamin D3 level in the experimental group was 18.19±7.84 ng/mL,and the IgE level was 551.51±705.88 IU/mL.The serum 25-hydroxyvitamin D3 level in the control group was 21.95±7.834 ng/mL,IgE level was 49.39±32.46 IU/mL.The data comparison indicates that the serum 25-hydroxyvitamin D3 level in the control group was higher than the experimental group,and the IgE level in the control group was lower than the experimental group(P<0.05).Conclusion:The level of vitamin D3 in patients with allergic symptoms is relatively low.This may be one of the causes leading to allergies.At the same time,IgE is also one of the causes contributing to allergies.An elevation of the IgE level is very likely to cause the allergic,and there is a correlation between serum vitamin D and IgE levels.At the same time,research has found that proper supplementation of vitamin D3 can help prevent allergic diseases,and can be considered in a new and highquality program for the prevention of allergic diseases.
