High levels of serum interleukin-6 increase mortality of hepatitis B virus-associated acute-on-chronic liver failure

BACKGROUND Patients with hepatitis B virus-associated acute-on-chronic liver failure(HBVACLF)present a complex and poor prognosis.Systemic inflammation plays an important role in its pathogenesis,and interleukin-6(IL-6)as a pro-inflammatory cytokine is related with severe liver impairment and also plays a role in promoting liver regeneration.Whether serum IL-6 influences HBV-ACLF prognosis has not been studied.AIM To determine the impact of serum IL-6 on outcome of patients with HBV-ACLF.METHODS We performed a retrospective study of 412 HBV-ACLF patients.The findings were analyzed with regard to mortality and the serum IL-6 level at baseline,as well as dynamic changes of serum IL-6 within 4 wk.RESULTS The serum IL-6 level was associated with mortality.Within 4 wk,deceased patients had significantly higher levels of IL-6 at baseline than surviving patients[17.9(7.3-57.6)vs 10.4(4.7-22.3),P=0.011].Patients with high IL-6 levels(>11.8 pg/mL)had a higher mortality within 4 wk than those with low IL-6 levels(≤11.8 pg/mL)(24.2%vs 13.2%,P=0.004).The odds ratios calculated using univariate and multivariate logistic regression were 2.10(95%confidence interval[CI]:1.26-3.51,P=0.005)and 2.11(95%CI:1.15-3.90,P=0.017),respectively.The mortality between weeks 5 and 8 in patients with high IL-6 levels at 4 wk was 15.0%,which was significantly higher than the 6.6%mortality rate in patients with low IL-6 levels at 4 wk(hazard ratio=2.39,95%CI:1.05-5.41,P=0.037).The mortality was 5.0%in patients with high IL-6 levels at baseline and low IL-6 levels at 4 wk,7.5%in patients with low IL-6 levels both at baseline and at 4 wk,11.5%in patients with low IL-6 levels at baseline and high IL-6 levels at 4 wk,and 16.7%in patients with high IL-6 levels both at baseline and at 4 wk.The increasing trend of the mortality rate with the dynamic changes of IL-6 was significant(P for trend=0.023).CONCLUSION A high level of serum IL-6 is an independent risk factor for mortality in patients with HBV-ACLF.Furthermore,a sustained high level or dynamic elevated level of serum IL-6 indicates a higher mortality.

Mild hypothermia effects on intercellular adhesion molecule-1 and serum interleukin-6 expression in brain tissues of a rat focal ischemia model

BACKGROUND： Previous studies have confirmed the neuroprotective effect of mild hypothermia on ischemic brain injury. OBJECTIVE： To investigate the effects of mild hypothermia on intercellular adhesion molecule-1 expression and serum interleukin-6 levels in ischemic brain tissues of focal brain ischemia rats, and to explore the neuroprotective effects of mild hypothermia on ischemic brain injury. DESIGN, TIME AND SETTING： A randomized, controlled, neurobiological experiment was performed at the Central Laboratory, First Affiliated Hospital, Xinxiang Medical College, China from February to July 2006. MATERIALS： Thirty healthy, adult, Sprague Dawley rats were used to establish middle cerebral artery occlusion models using the suture method, The immunohistochemistry （streptavidin-biotin-peroxidase complex method） kit was purchased from Boster, China. Interleukin-6 radioimmunoassay was supplied by Institute of Radioimmunity, Technology Development Center, General Hospital of Chinese PLA. METHODS： The rats were equally and randomly assigned into mild hypothermia and control groups, and middle cerebral artery occlusion models were established. The rectal temperature was maintained at （37 ±0.5）℃ in the control group. In the mild hypothermia group, the rectal temperature was maintained at （33±1）℃. MAIN OUTCOME MEASURES： At 12 hours after model establishment, the ischemic brain hemispheres were coronally sliced at the level of the optic chiasm. The number of intercellular adhesion molecule-1-positive vessels per high-power field was observed with an optical microscope. Serum interleukin-6 levels were measured by radioimmunoassay. RESULTS： Compared with the control group, intercellular adhesion molecule-1 and serum interleukin-6 expressions were significantly decreased in ischemic brain tissues of the mild hypothermia group （P 〈 0.01）. CONCLUSION： Mild hypothermia exhibits a neuroprotective effect by reducing serum interleukin-6 and intercellular adhesion molecule-1 expression following cerebral ischemia.

Influence of Sancai and conventional acupuncture manipulations on injured sciatic nerve histomorphology and serum interleukin-6 expression

Acupuncture and moxibustion therapy non-specifically raises the pain threshold, relieves regional muscular tension, improves local blood circulation, accelerates elimination of algogenic substances such as acid metabolites, and promotes neural regeneration and functional rehabilitation. However, the effects of acupuncture are synthetically influenced by multiple factors, which vary with acu-puncture point, intensity, and manipulation. The present study explored the analgesic effects and mechanisms of specific Sancai acupuncture manipulation for sciatica. Results revealed that Sancai acupuncture manipulation significantly reduced inflammatory factor interleukin-6 expression in the blood serum of rats with sciatica, exhibited anti-inflammatory effects and promoted rehabilitation of injured nerves. In addition, Sancai acupuncture manipulation exhibited superior curative effects over conventional acupuncture manipulation.

Diagnostic value of serum Interleukin-6 combined with synovial IL-6 and C-reactive protein for prosthesis joint infection in patients after joint arthroplasty

Objective:To explore the diagnostic value of serum Interleukin-6(IL-6)combined with synovial IL-6 and C-reactive protein for prosthesis joint infection(PJI)in patients after joint arthroplasty.Methods:A total of 253 patients undergoing revision surgery after the first total hip and total knee arthroplasty in the Second Affiliated Hospital of Hainan Medical College from January 2018 to April 2020 were recruited as the study object.The patients were divided into the PJI group(n=85)and non-PJI group(n=168)according to whether there was PJI,and the baseline characteristics and the laboratory indexe were collected and compared between the two groups.the factors associated with PJI in patients after joint arthroplasty were analyzed by multivariate logistic regression model.And the diagnostic value of each marker for PJI in patients after joint arthroplasty was evaluated by Receiver Operating Characteristic(ROC)curve.Results:The incidence of PJI in this study was 33.6%.Compared with the non-PJI group,the patients in the PJI group had more diabetes mellitus and shorter prosthesis duration(P<0.05).The levels of serum C-reactive protein(CRP),serum interleukin-6(IL-6),synovial IL-6,synovial CRP,synovial leukocyte,synovial neutrophil and ESR in the PJI group were higher than those in non-PJI group,and the level of synovial lymphocyte was lower,with statistically significant differences(P<0.05).Multivariate logistic regression analysis showed that diabetes mellitus(OR=1.706,95%CI:1.02~2.376,P=0.037),prosthesis duration(OR=0.781,95%CI:0.690~0.884,P<0.001),serum IL-6(OR=1.008,95%CI:1.004~1.012,P=0.046),synovial IL-6(OR=1.004,95%CI:1.002~1.006,P=0.011)and synovial CRP(OR=1.481,95%CI:1.010~2.170,P=0.044)were independently related to PJI in patients after joint arthroplasty.ROC curve analysis showed that the area under ROC curve(AUC)of serum IL-6 combined with synovial IL-6 and CRP for diagnose PJI in patients after joint arthroplasty was significantly greater than each marker(0.941 vs 0.760,Z=6.333,P<0.001;0.941 vs 0.743,Z=7.189,P<0.001;0.941 vs 0.785,Z=4.999,P=0.006).Conclusion:Serum IL-6,synovial IL-6 and CRP were independently related to PJI in patients after joint arthroplasty.They all had some diagnostic value,and serum IL-6 combined with synovial IL-6 and CRP had higher capability to diagnose PJI in patients after joint arthroplasty than each marker alone.

Effect of flurbiprofen combined with prednisolone on interleukin-6 in elderly surgery patients

Objective: To determine the effect of flurbiprofen combined with prednisolone on interleukin-6 in elderly surgery patients. Methods: In this double-blind randomized controlled study, patients aged 65 to 80 who were undergoing spinal fusion surgery for disc herniation were administered flurbiprofen 100 mg (P group, flurbiprofen group), prednisolone 0.6 mg/kg (D group, prednisolone group), prednisolone 0.6 mg/kg plus flurbiprofen 100 mg (P + D group, flurbiprofen + prednisolone group) or normal saline (S group, saline group) 15 minutes before the induction of anesthesia. Plasma samples were collected before surgery (T0) and on day 1 (T1), day 2 (T2) and day 3 (T3) following surgery. At the same time, systemic inflammatory response syndrome (SIRS) was assessed by SIRS criteria. The levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and C-reactive protein (CRP) for collected samples were measured. Results: Other groups had significantly lower levels of IL-6, CRP and occurrence of SIRS than S group (p < 0.05). Compared to groups P and D, the levels of IL-6 and CRP in P + D group were significantly lower on T1 (p < 0.05). Peak levels of IL-6 in all groups were presented on T1 (p 0.05). The levels of CRP within three days were significantly different but did not show peak levels (p > 0.05). Conclusion: Compared to prednisolone or flurbiprofen, combining flurbiprofen with prednisolone in elderly surgery patients led to an increased suppression of IL-6.

Clinical analysis between serum 25-hydroxyvitamin D3, interleukin-6 levels and febrile convulsion in children

Objective:To investigate the relation between febrile convulsions and 25hydroxy-vitamin D_(3)[25-(OH)D_(3)]and interleukin-6(IL-6)levels in children.Methods:241 children(divided into simple febrile convulsions and complex febrile convulsions),who were diagnosed with febrile convulsions at the Women and Children's Medical Center of Hainan Province from January 2017 to October 2022,were selected into the febrile convulsions group;100 healthy children,who had no uncomfortable symptoms and attended the outpatient clinic of the Women and Children's Medical Center of Hainan Province for physical examination,for the control group.All the subjects measured the serum 25-(OH)D_(3) and IL-6 levels,and clinical information,such as age,gender and season,was recorded.Results:1)Serum 25-(OH)D_(3) levels in the febrile convulsion group were significantly lower than in the healthy control group(78.77±20.37 nmol/L versus 96.55±29.74 nmol/L,respectively),and there was a statistically significant between the two groups(t value-6.359,P<0.001).Serum IL-6 levels in the febrile convulsion group were significantly higher than in the healthy control group,and there was a statistically significant between the two groups(Z value of-14.291,P<0.001).2)Serum 25-(OH)D_(3) levels in children with complex febrile convulsions were significantly lower than those in children with simple febrile convulsions,and the difference between the two groups was statistically significant(t-value of 6.612,P<0.05).IL-6 levels were higher in children with complex febrile convulsions than in children with simple febrile convulsions,and the difference between the two groups was statistically significant(Z value-10.151,P<0.001).The difference in the severity of febrile convulsions was statistically significant in serum 25-(OH)D_(3) levels(x^(2)=29.83,P<0.001).3)The results of correlation analysis showed that serum 25-(OH)D_(3) level was negatively correlated with febrile convulsion(γ=-0.393,P<0.05);serum 25-(OH)D_(3) level was positively correlated with that(γs=0.328,P<0.05).4)The correlation analysis results showed that the serum 25-(OH)D_(3) level was negatively correlated with the clinical characteristics of febrile convulsion(γ=-0.393,P<0.05).However,serum IL-6 water is positively correlated with it(γs=0.328,P<0.05).4)In contrast,there was no statistically significant difference in serum 25-(OH)D_(3) levels among children with febrile convulsions in different seasons(P>0.05).Conclusions:There is a correlation between febrile convulsion and serum levels of 25-(OH)D_(3) and IL-6.25-(OH)D_(3) and IL-6 may participate in the pathogenesis of febrile convulsion.

Association between Metabolic Syndrome Components and Serum High-Sensitivity C-Reactive Protein or Interleukin-6 Levels among Congolese Adults

Metabolic syndrome (MetS) and its components have been linked to elevated serum levels of inflammatory biomarkers such as C-reactive protein, interleukin-6, interleukin-1β and tumor necrosis factor alpha. The aim of our study was to address the association between MetS components with serum hs-CRP and IL-6 levels among Congolese adults. A total of 357 participants (aged 30 - 87 years) were included in this cross-sectional study. Anthropometrics were collected and fasting blood sampled for assessment of fasting blood glycaemia (FBG), lipids and inflammatory parameters using commercially available assays. NCEP-ATPIII criteria were used to define MetS. The Median (IQR) hs-CRP and IL-6 levels were higher in participants with MetS than in those without ([7 (4, 14) versus 6 (4, 8)] mg/L;p = 0.092 and [23.8 (20.9, 27.6) versus 22.3 (19.5, 25.0)] pg/mL;p = 0.002). hs-CRP and IL-6 levels were significantly higher in females with MetS than in those without, but not in males. Among participants, only TG was correlated with hs-CRP (r = 0.149, p = 0.007), and a significant correlation was observed between TG (r = 0.116, p = 0.037), FBG (r = 0.208, p = 0.000), HDL-C (r = −0.119, p = 0.034) and SBP (r = 0.143, p = 0.010) and IL-6. In males, hs-CRP levels were positively correlated with TG (0.316;p = 0.000), negatively with HDL-C (r = −0.290, p = 0.0022), without such correlations in females. In Ames, IL-6 levels were positively correlated with FBG (r = 0.202;p = 0.035), and negatively with HDL-C (r = −0.249, p = 0.009). Significant correlations between IL-6 levels and FBG (r = 0.214;p = 0.000) or SBP (r = 0.227, p = 0.000) were observed in females. Logistic regression analysis was carried out to identify the relationship between MetS components and hs-CRP or IL-6. Values of area under receiver-operating characteristic (ROC) curves suggest potential use of serum hs-CRP (AUC = 0.675) and IL-6 (AUC = 0.656) as diagnostic biomarkers of MetS. Combination of hs-CRP and IL-6 improved diagnosis accuracy, yielding a 0.698 ROC curve area. MetS components are associated with hs-CRP and IL-6 levels among adults Congolese. Combining the two biomarkers hs-CRP and IL-6 improves Mets diagnostic accuracy compared to hs-CRP or IL-6 alone.

降尿酸方辅助治疗痛风伴高尿酸血症的临床疗效及对血清IL-1β、IL-6的影响

目的探究降尿酸方辅助治疗痛风伴高尿酸血症的临床疗效及对血清白细胞介素-6(interleukin-6,IL-6)、白细胞介素-1β(interleukin-1β,IL-1β)的影响。方法以2021年1月—2021年12月该院收治的80例痛风伴高尿酸血症患者为研究对象,依据随机数字表分入观察组40例和对照组40例。对照组给予非布司他片口服,观察组在对照组基础上加用降尿酸方辅助治疗。比较两组治疗疗效、不良反应及治疗前后痰浊阻滞证中医证候积分、血尿酸水平、血清炎性因子变化情况。结果与对照组(80.00%,32/40)相比,观察组治疗总有效率(95.00%,38/40)更高(P<0.05);两组治疗后关节肿胀、局部酸麻疼痛、目眩、面浮足肿、胸脘痞闷等中医证候较治疗前均降低,且观察组中医证候积分均低于对照组,差异有统计学意义(P<0.05);两组治疗后血尿酸、血清IL-6、IL-1β、肿瘤坏死因子-α(TNF-α)水平较治疗前均降低,且观察组低于对照组,差异有统计学意义(P<0.05);观察组与对照组不良反应[15.00%(6/40)vs 10.00%(4/40)]比较差异无统计学意义(P>0.05)。结论降尿酸方辅助治疗痛风伴高尿酸血症疗效确切,可降低患者中医证候积分,降低血尿酸及血清IL-6、IL-1β水平,改善机体炎症反应,值得临床推广应用。

神丹6号绿壳蛋鸡10~18周龄血脂代谢规律研究

试验旨在研究神丹6号绿壳蛋鸡10~18周龄血脂代谢情况。试验以神丹6号绿壳蛋鸡10周龄商品代鸡为素材,以海兰褐蛋鸡为对照,饲养至18周龄,每周采血测量血清中脂质代谢相关生化指标,并进行差异分析和相关性分析。结果显示,随着周龄增长,神丹6号绿壳蛋鸡血清游离脂肪酸(FFA)含量整体降低,高密度脂蛋白(HDL)和低密度脂蛋白(LDL)含量先降低,之后保持不变,甘油三酯(TG)、总胆固醇(TC)、极低密度脂蛋白y(VLDLy)和卵黄蛋白原(VTG)含量基本保持不变。与海兰褐蛋鸡相比,神丹6号绿壳蛋鸡血清FFA含量明显较低,其他指标含量则与海兰褐基本相当。10周龄与18周龄比较分析结果显示,脂代谢指标中仅FFA在神丹6号绿壳蛋鸡和海兰褐蛋鸡中极显著差异,腹脂率和肝脏指数仅在神丹6号绿壳蛋鸡中显著差异。神丹6号绿壳蛋鸡血清FFA含量与TG、VLDLy显著负相关;HDL含量与LDL负相关,但并不显著。综合分析表明,神丹6号绿壳蛋鸡10~18周龄血脂代谢水平稳定,血脂代谢比高产蛋鸡慢。

苏禽6号蛋鸡早期血脂代谢规律研究

旨在研究苏禽6号蛋鸡早期血脂代谢情况,为该配套系的早期饲养管理和营养调控提供理论依据。以苏禽6号蛋鸡10周龄商品代鸡为试验组,海兰褐蛋鸡为对照组,饲养至18周龄,每周采血测量血清中脂质代谢相关生化指标并进行差异分析和相关性分析。结果显示:随着周龄增加,苏禽6号蛋鸡腹脂率前期不变,后期升高;肝脏指数前期降低,后期不变;血清游离脂肪酸(FFA)含量整体降低,总胆固醇(TC)和低密度脂蛋白(LDL)含量波动变化,卵黄蛋白原(VTG)含量先降低后基本保持不变。与海兰褐蛋鸡相比,苏禽6号蛋鸡腹脂率、血清甘油三酯(TG)含量整体较高,TC含量时高时低,高密度脂蛋白(HDL)含量则较低,而VTG含量前期基本一致,后期则明显较低。前期与后期比较分析结果显示,脂代谢指标中仅FFA含量在苏禽6号蛋鸡和海兰褐蛋鸡中均差异显著(P<0.05),腹脂率和肝脏指数仅在苏禽6号蛋鸡中差异显著(P<0.05)。苏禽6号蛋鸡血清TG含量与极低密度脂蛋白y(VLDLy)、腹脂率极显著正相关(P<0.01),与FFA显著负相关(P<0.05);TC、HDL和LDL三者之间均极显著正相关(P<0.01)。综合分析表明,苏禽6号蛋鸡早期血脂代谢稳定并且比高产蛋鸡慢。

血清IgG、白细胞介素-6、维生素D检测在肺炎支原体感染合并支气管哮喘患儿中的应用研究

目的:分析血清免疫球蛋白G(IgG)、白细胞介素-6(IL-6)、维生素D检测在肺炎支原体(MP)感染合并支气管哮喘患儿中的应用价值.方法:选取2018年1月—2022年8月广州医科大学附属第二医院儿科住院部收治的MP感染患儿103例为研究对象,根据是否合并支气管哮喘,分为未合并哮喘组(n=68)及合并哮喘组(n=35).分别采用酶联免疫法、电化学发光法、液相色谱串联质谱检测患儿血清IgG、IL-6、维生素D水平.结果:合并哮喘组血清IgG、IL-6水平高于未合并哮喘组,维生素D水平低于未合并哮喘组,差异有统计学意义(P<0.001);Pearson相关系数分析结果显示,MP感染合并支气管哮喘发生率与血清IgG、IL-6水平呈正相关(r=0.925,P=0.029;r=0.931,P=0.048),与血清维生素D水平呈负相关(r=-0.964,P=0.021).结论:血清IgG、IL-6、维生素D水平可有效反映MP感染合并支气管哮喘患儿的病情,为其后续治疗提供参考依据.

神丹6号绿壳蛋鸡和海兰褐蛋鸡产蛋期腹脂和血清脂代谢指标的比较研究

旨在比较神丹6号绿壳蛋鸡和海兰褐蛋鸡产蛋期腹脂和血脂代谢情况,为神丹6号绿壳蛋鸡的产蛋期饲养管理和营养调控提供理论依据。神丹6号绿壳蛋鸡和海兰褐蛋鸡19周龄商品代鸡,饲养至60周龄,19~28周龄每周采血一次,29~40周龄每2周采血一次,41~60周龄每4周采血一次,测量血清中脂质代谢相关生化指标,进行差异分析和相关性分析。结果显示:随着周龄增长,神丹6号绿壳蛋鸡腹脂率和肝脏指数变化不显著;血清甘油三酯(TG)、游离脂肪酸(FFA)和高密度脂蛋白(HDL)含量先升高后降低,之后变化不显著或波动变化;低密度脂蛋白(LDL)先降低后升高,之后变化不显著;总胆固醇(TC)含量整体降低;极低密度脂蛋白y(VLDLy)和卵黄蛋白原(VTG)含量前期变化不显著,之后波动变化。随着周龄增长,海兰褐蛋鸡血清HDL、LDL、VLDLy和VTG含量变化与神丹6号绿壳蛋鸡趋势略有差异,其他指标趋势基本一致。与海兰褐蛋鸡相比,神丹6号绿壳蛋鸡血清FFA和VTG含量前期较低,后期均与其含量接近,TG、TC、HDL、LDL和VLDLy含量则整体相近。产蛋前期(19周龄)、高峰期(28周龄)与后期(52周龄)比较分析结果显示,脂代谢指标中TG和FFA在神丹6号绿壳蛋鸡产蛋高峰期显著高于前期和后期(P<0.05),HDL和VTG均是后期显著低于前期(P<0.05)。综合分析表明,神丹6号绿壳蛋鸡产蛋期血脂代谢稳定,与海兰褐蛋鸡相比前期慢,后期相当。

血清和脑脊液IL-6与IL-10检测在新生儿化脓性脑膜炎中的意义

目的 探讨血清和脑脊液白细胞介素-6(IL-6)、白细胞介素-10(IL-10)在新生儿化脓性脑膜炎(NPM)中的临床意义。方法 采用病例对照研究的方法,分析2021年9月至2022年11月昆明市儿童医院新生儿科收治的有腰椎穿刺指征的95例患儿。根据入院24 h内第1次腰椎穿刺脑脊液检查结果将95例患儿分为NPM组和脓毒症组。分别检测所有患儿入院后第1天血清和脑脊液IL-6、IL-10水平;NPM组治疗后第3、10天进行腰椎穿刺复查,收集脑脊液,除进行脑脊液常规及生化检查外,还采集血液标本,检测血清和脑脊液中IL-6、IL-10水平。比较脓毒症组和NPM组患儿第1天血清和脑脊液IL-6、IL-10的水平;比较NPM组患儿入院第1、3、10天血清和脑脊液IL-6、IL-10水平。结果 NPM组45例和脓毒症组50例。脓毒症组和NPM组脑脊液白细胞计数及蛋白、葡萄糖水平比较,差异有统计学意义(P<0.05)。入院第1天,脓毒症组和NPM组患儿血清IL-6、IL-10水平比较,差异均无统计学意义(P>0.05);NPM组脑脊液IL-6、IL-10水平明显高于脓毒症组(P<0.05)。入院第1天,脓毒症组血清IL-6水平与脑脊液IL-6水平比较,差异无统计学意义(P>0.05),而血清IL-10水平明显高于脑脊液IL-10水平(P<0.05)。NPM组第1天血清IL-6、IL-10水平明显高于第10天(P<0.05);第1天与第3天脑脊液IL-6、IL-10水平比较,差异无统计学意义(P>0.05);第1天与第3天脑脊液IL-6、IL-10水平均明显高于第10天(P<0.05)。NPM组患儿入院第1、3、10天的脑脊液IL-6水平分别高于同期血清IL-6水平(P<0.05)。NPM组患儿入院第1、3天的脑脊液IL-10水平分别高于同期血清IL-10水平(P<0.05),但第10天血清IL-10水平与脑脊液IL-10水平相比,差异无统计学意义(P>0.05)。入院第1天血清IL-6、IL-10诊断NPM的曲线下面积(AUC)分别为0.544、0.552,对NPM的诊断效能低。入院第1天脑脊液IL-6和IL-10诊断NPM的AUC分别为0.897、0.883,对NPM有一定的诊断价值。结论 检测脑脊液IL-6、IL-10水平有助于辅助诊断NPM;动态监测脑脊液IL-6、IL-10水平对NPM治疗效果的判定有一定临床意义。

High serum interleukin-6 level is associated with increased risk of delirium in elderly patients after noncardiac surgery： a prospective cohort study

Background The relationship between inflammation and delirium remains to be determined.The purposes of this study were to investigate the association between serum interleukin-6 levels and the occurrence of delirium in elderly patients after major noncardiac surgery.Methods A total of 338 elderly patients （60 years of age and over） undergoing major noncardiac surgery were enrolled.Blood samples were obtained before anesthesia and in the first postoperative morning and serum interleukin-6 concentrations were measured.Delirium was assessed twice daily by the confusion assessment method for the Intensive Care Unit during the first three postoperative days.Survival analyses were performed to assess the relationship between the serum IL-6 level and the occurrence of postoperative delirium.Results Postoperative delirium occurred in 14.8% （50 of 338） of patients.High serum interleukin-6 levelsafter surgery were significantly associated with increased risk of the occurrence of postoperative delirium （hazard ratio 1.514,95% confidence interval 1.155-1.985,P=0.003）.Other independent predictors of delirium included increasing age,poor preoperative New York Heart Association classification,low preoperative Mini-Mental State Examination score,and high total postoperative Visual Analogue Scale pain score.Patients who developed delirium had a prolonged hospital stay after surgery.Conclusions Delirium is a frequent complication in elderly patients after noncardiac surgery.High serum interleukin-6 level after surgery is associated with increased risk of the occurrence of postoperative delirium.

血清淀粉样蛋白A、白细胞介素-6及T淋巴细胞亚群相关指标在系统性红斑狼疮活动期的表达水平分析

目的:分析血清淀粉样蛋白A(SAA)、白细胞介素-6(IL-6)及T淋巴细胞亚群相关指标在系统性红斑狼疮(SLE)活动期的表达水平。方法:选取2020年10月—2023年5月玉林市第一人民医院收治的SLE患者200例作为研究对象,根据SLE活动指数积分表评分分为SLE稳定期组与SLE活动期组,各100例,另选取同期于玉林市第一人民医院进行健康体检的健康人100例作为对照组。检测不同人群SAA、IL-6及T淋巴细胞亚群相关指标水平。结果:SLE稳定期组及SLE活动期组CD3%、CD8%、SAA、IL-6水平均高于对照组,SLE稳定期组SAA、IL-6水平低于SLE活动期组,SLE稳定期组CD4%高于对照组与SLE活动期组,差异有统计学意义(P<0.05)。SLE稳定期组及SLE活动期组CD3^(+)、CD4^(+)、CD8^(+)均小于对照组,且SLE活动期组CD3^(+)、CD4^(+)、CD8^(+)均小于SLE稳定期组,差异有统计学意义(P<0.05)。结论:SAA、IL-6及T淋巴细胞亚群相关指标在SLE患者中均有异常改变,SLE患者活动期T淋巴细胞亚群绝对计数低于稳定期患者,对于SLE的临床诊治具有一定参考价值。

Serum Interleukin-6 Level and the rs1800795 Polymorphism in its Gene Associated with Neuroblastoma Risk in Chinese Children

Background：The pro-inflammatory cytokine,interleukin-6 （IL-6）,stimulates the metastasis of several neoplasms.An association of its serum level and the single nucleotide polymorphism （SNP） rs1800795 with neuroblastoma （NB） has been reported in American and Italian cohorts.This study was to clarify whether the same association exists in Chinese children.Methods：A total of 130 NB patients,with 77 boys （59％）,53 girls （41％）,mean age 41 ± 5 months,were assigned to two groups：high risk （HR） versus intermediate-low risk （non-HR）,and 50 healthy children were randomly selected as the age-and gender-matched controls.Peripheral blood samples were analyzed to determine serum IL-6 level using enzyme linked immunosorbent assay and rs1800795 SNPs phenotype using polymerase chain reaction and gene sequencing.Results：There were 87 NB patients in the HR group and 43 NB patients in the non-HR group.A comparison of allele and genotype frequencies of the rs1800795 polymorphism between patients and controls found no association with NB risk （P ＞ 0.05）.The frequency of GG＋GC genotype was higher in HR-NB patients than in non-HR-NB patients （64.4％ vs.48.8％,P =0.02）,and serum IL-6 level was much higher in HR-NB patients with GG＋GC genotype than in HR-NB patients with CC genotype （4.36 ± 1.1 pg/ml vs.1.83 ± 0.5 pg/ml;P =0.02）,but not in Non-HR-NB patients.Conclusions：The polymorphism rs1800795 is associated with serum IL-6 level and level of NB risk.GG genotype might indicate that the tumor is highly malignant （prone to metastasis） and associated with poor prognosis.

The diagnostic value of transient elastography combined with serum SAA and IL-6 in the degree of hepatitis B liver fibrosis

Objective:To investigat the diagnostic value of transient elastography combined with serum amyloid A and interleukin-6 in the degree of hepatitis B liver fibrosis.Methods:A total of 334 patients with chronic HBV infection that were admitted to the Department of Infectious Diseases of the First Affiliated Hospital of Hainan Medical College from January 2020 to May 2022 with informed consent and underwent liver biopsy puncture were selected.According to the pathological results,they were divided into no obvious fibrosis group,obvious fibrosis group and liver cirrhosis group.Comparison of liver stiffness measurement(LSM),serum amyloid A(SAA0,IL-6 levels between different groups.This study drawed was conducted draw the receiver operating characteristic(ROC)curve of each index to diagnose significant liver fibrosis and liver cirrhosis,and compared the area under the ROC curve(AUC)and diagnostic efficacy of each non-invasive fibrosis diagnostic model.The diagnostic performance of the combined assay was superior to that of APRI and FIB-4 In different degrees of liver fibrosis.Results:According to the degree of liver fibrosis,the levels of SAA,IL-6,and LSM in the no significant fibrosis group(n=140),the significant fibrosis group(n=134),and the cirrhosis group(n=60)were statistically significant difference(All P<0.001).SAA,IL-6 and LSM were significantly correlated with the degree of liver fibrosis(rs=0.456,rs=0.482,rs=0.602,All P<0.001).The AUC of SAA and IL-6 for the diagnosis of significant fibrosis in hepatitis B were 0.738 and 0.809,respectively.And the AUC for the diagnosis of liver cirrhosis were 0.813 and 0.823,respectively.The AUC for the combined diagnosis of significant fibrosis and cirrhosis were 0.930 and 0.964,respectively.The diagnostic performance of the combined assay was superior to that of APRI and FIB-4 in different degrees of liver fibrosis(All P<0.001).Conclusion:LSM combined with serum SAA and IL-6 has great diagnostic value for different degrees of hepatitis B liver fibrosis.

IL-6、SAA、CRP及PCT联合检测对肺炎支原体合并细菌感染患者的早期诊断价值

目的探讨白细胞介素-6(IL-6)、血清淀粉样蛋白A(SAA)、C反应蛋白(CRP)及血清降钙素原(PCT)联合检测对肺炎支原体合并细菌感染患者的早期诊断价值。方法选取疑似合并细菌感染的肺炎支原体患者150例,根据血培养结果分为观察组(确诊为肺炎支原体合并细菌感染,85例)和对照组(单纯肺炎支原体感染,65例)。收集所有患者血培养当天的血清标本,检测血清中的IL-6、SAA、CRP及PCT水平。比较两组患者IL-6、SAA、CRP及PCT水平,分析四项指标对肺炎支原体合并细菌感染的诊断效能,分析四项指标联合诊断肺炎支原体合并细菌感染的价值。结果观察组患者血清IL-6、SAA、CRP及PCT分别为(238.57±21.57)pg/ml、(169.68±17.66)mg/L、(77.98±6.58)mg/L、(13.58±2.04)ng/ml,均高于对照组的(137.25±18.07)pg/ml、(128.90±15.71)mg/L、(52.60±5.87)mg/L、(8.24±1.87)ng/ml,,差异有统计学意义(P<0.05)。IL-6的诊断特异性、敏感性、约登指数、阴性预测值、阳性预测值分别为92.31%、83.53%、0.7584、81.08%、93.42%,SAA的分别为83.08%、74.12%、0.5720、71.05%、85.14%,CRP的分别为86.15%、90.59%、0.7674、87.50%、89.53%,PCT的分别为70.77%、71.76%、0.4253、65.71%、76.25%;CRP的约登指数最高,PCT的约登指数最低。串联检测的诊断特异性、敏感性、约登指数、阴性预测值、阳性预测值分别为70.77%、71.76%、0.4253、65.71%、76.25%,并联检测的分别为92.31%、94.12%、0.8643、92.31%、94.12%,并联检测的约登指数高于串联检测。结论与单纯肺炎支原体感染患者相比,肺炎支原体合并细菌感染患者的血清IL-6、SAA、CRP及PCT水平有升高趋势。联合检测四项指标有助于早期诊断肺炎支原体合并细菌感染,对于减少漏诊、帮助医务人员制定正确的治疗方案具有意义。

Diagnostic performance of serum interleukin-6 in pediatric acute appendicitis:a systematic review

Background Despite the radiological and analytical advances of the last decades,there is still a significant rate of diagnostic error in pediatric acute appendicitis.In recent years,multiple biomarkers have emerged as potential diagnostic tools.This study aimed to examine the diagnostic performance of serum interleukin-6 in pediatric acute appendicitis.Methods We conducted a systematic review of the literature that involved an extensive search in the main databases of medical bibliography(Medline,PubMed,Web of Science and SciELO).Two independent reviewers selected the relevant articles based on the previously defined inclusion and exclusion criteria.Methodological quality of the selected article was rated using the QUADAS2 index.Data extraction was performed by two independent reviewers.Results The research in the medical bibliography databases resulted in 68 articles.We removed 26 duplicates.Among the remaining 42 articles,we excluded 33 following the inclusion and exclusion criteria.Of the final 9 studies included in this review,8 provided measured serum interleukin-6 values,and all of them reported significant differences between groups,but inconsistent results regarding sensitivity and specificity.Conclusions The diagnostic performance of interleukine-6 alone for the diagnosis of acute appendicitis in children is limited.The sensitivity and specificity of interleukine-6 for the diagnosis of non-complicated acute appendicitis in the pediatric population are moderate but increased in complicated appendicitis.There seems to be a direct relationship between serum level of interleukin-6 and the hours of evolution of abdominal pain in children with acute appendicitis.The increasingly widespread use of non-operative management of acute appendicitis warrants further exploration of the classificatory potential of this marker between complicated and uncomplicated appendicitis.We consider that this may be an avenue of research to explore in the future.

6-Gingerol, asarinin, and deoxyschizandrin improve bronchial epithelium functions in an interleukin-13einduced BEAS-2B cell model

Objective:To explore the effects of 6-gingerol,asarinin,and deoxyschizandrindthe main components of Zingiber officinale(Willd.)Rosc.(Gan Jiang),Asarum heterotropoides f.var.mandshuricum(Maxim.)(Xi Xin),and Schisandra chinensis(Turcz.)Baill.(Wu Wei Zi),respectivelydon an interleukin(IL)-13einduced BEAS-2B cell model in vitro.Methods:The BEAS-2B cell model was established using 25 ng/mL IL-13 combined with 1%fetal bovine serum(FBS)in vitro.Mitoquinone mesylate(Mito-Q)treatment was used as a positive control group,and different concentrations of 6-gingerol,asarinin,and deoxyschizandrin were used to treat the models.The level of reactive oxygen species(ROS)production was detected by flow cytometry.The expression levels of LC3B,Beclin1,adenosine 50-monophosphate(AMP)eactivated protein kinase(AMPK),phosphory-lated-AMPeactivated protein kinase(P-AMPK),dynamin-related protein 1(DRP1),and mitochondrial fusion protein 2(MFN2)were detected by Western blot.Mitochondrial membrane potential(MMP)assay kit with JC-1 was utilized to detect the level of MMP.Results:The BEAS-2B cells exposed to 25 ng/mL IL-13 with 1%FBS showed an increased ROS level and a decreased MMP.6-Gingerol,asarinin,and deoxyschizandrin were able to downregulate ROS level and upregulate the MMP in the BEAS-2B model.Asarinin and deoxyschizandrin reduced the expression of autophagy protein LC3B,while deoxyschizandrin significantly increased the expression of DRP1 in the BEAS-2B model.Conclusion:6-Gingerol,asarinin,and deoxyschizandrin can reduce ROS generation and increase MMP,but have different regulatory effects on the expression of autophagy protein and mitochondrial mitotic protein.The three components have both synergistic and complementary effects in classic medicine compatibility.This study may provide an innovative strategy to reduce the lung inflammation related to IL-13.