Hypochromic microcytic anaemia includes iron deficiency, anaemia of chronic disorders, beta thalassemia trait and sideroblastic anaemia. To rule out the cause of hypochromic microcytic anaemia is a diagnostic difficul...Hypochromic microcytic anaemia includes iron deficiency, anaemia of chronic disorders, beta thalassemia trait and sideroblastic anaemia. To rule out the cause of hypochromic microcytic anaemia is a diagnostic difficulty. The conventional laboratory tests used for diagnosis have few disadvantages. Serum transferrin receptor (sTfR) is the most reliable method for assessment of body iron. Eighty four children were included in this study. They were further divided into four groups: iron deficiency anaemia (IDA), anaemia of chronic disorders (ACD), beta thalassemia trait (β TT) and controls. Children withIDAand ACD were diagnosed on the basis of history and serum iron profile. Subjects with β TT had HbA2 > 3.5%. sTfR were performed on all subjects. Level of sTfR in patients withIDAwas 5.79 μg/ml ± 1.3 μg/ml. In patients with anaemia of chronic disorders (ACD), β thalassemia trait and controls mean sTfR were 2.18 μg/ml ± 0.6 μg/ml, 2.1μg/ml ± 0.5 μg/ml and 2.0 μg/ml ± 0.5 μg/ml respectively. These results show level of sTfR was raised in IDA when compared with controls or ACD and β TT (p展开更多
Objective To establish and evaluate a protein serum ferritin （SF） and soluble transferrin receptor microarray method for combined measurement of （sTfR）. Methods Microarrayer was used to print both anti-SF antibodi...Objective To establish and evaluate a protein serum ferritin （SF） and soluble transferrin receptor microarray method for combined measurement of （sTfR）. Methods Microarrayer was used to print both anti-SF antibodies I and anti-sTfR antibodies I on each protein microarray. Anti-SF antibodies II and anti-sTfR antibodies II were used as detection antibodies and goat antibodies coupled to Cy3 were used as antibodies Ill. The detection conditions of the quantitative analysis method for simultaneous measurement of SF and sTfR with protein microarray were optimized and evaluated. The protein microarray was compared with commercially available traditional tests with 26 serum samples. Results By comparison experiment, mouse monoclonal antibodies were chosen as the probes and contact printing was chosen as the printing method. The concentrations of SF and sTfR probes were 0.5 mg/mL and 0.5 mg/mL respectively, while those of SF and sTfR detection antibodies were 5 μg/mL and 0.36 μg/mL respectively. Intra- and inter-assay variability was between 3.26% and 18.38% for all tests. The regression coefficients comparing protein microarray with traditional test assays were better than 0.81 for SF and sTfR. Conclusion The present study has established a protein microarray method for combined measurement of SF and sTfR.展开更多
目的:观察世居高原中长跑运动员高住低练(living high training low,HiLo)期间血清EPO、sTfR及血象指标变化,探讨HiLo训练对世居高原耐力项目运动员红细胞生成的影响。方法:随机将20名世居海拔2260m高原的中长跑运动员(男12名,女8名)分...目的:观察世居高原中长跑运动员高住低练(living high training low,HiLo)期间血清EPO、sTfR及血象指标变化,探讨HiLo训练对世居高原耐力项目运动员红细胞生成的影响。方法:随机将20名世居海拔2260m高原的中长跑运动员(男12名,女8名)分成低氧组和对照组,每组10名(6男,4女)。低氧组在模拟海拔4000m低压低氧(大气压力为60kPa,氧气浓度为20.95%)环境下进行HiLo实验,每天低氧暴露10小时(8:00pm～6:00am),白天在其居住生活的海拔2260m高度进行训练;对照组睡、练均在海拔2260m环境。两组受试者实验期间的训练负荷、时间及营养状况均相同。实验进行4周。分别于实验前,实验开始1天、每周及实验结束后2周抽静脉血,测试血清促红细胞生成素(EPO)、血清转铁蛋白受体(sTfR)及血红蛋白(Hb)、红细胞压积(Hct)。结果:4周HiLo后,低氧组运动员Hb、Hct较实验前无明显改善(P>0.05),EPO除在低氧暴露第1天比实验前有升高趋势(P<0.10)外,整个实验期间与实验前及对照组相比均无显著性改变;HiLo训练后低氧组sTfR与实验前和对照组相比也未发生有统计意义的变化。结论:世居高原(海拔2260m)中长跑运动员进行4周HiLo训练对其EPO、sTfR、Hb升高作用不显著,表明世居高原中长跑运动员4周HiLo训练(模拟海拔4000m睡眠,海拔2260m训练)对机体红细胞生成影响不明显。展开更多
文摘Hypochromic microcytic anaemia includes iron deficiency, anaemia of chronic disorders, beta thalassemia trait and sideroblastic anaemia. To rule out the cause of hypochromic microcytic anaemia is a diagnostic difficulty. The conventional laboratory tests used for diagnosis have few disadvantages. Serum transferrin receptor (sTfR) is the most reliable method for assessment of body iron. Eighty four children were included in this study. They were further divided into four groups: iron deficiency anaemia (IDA), anaemia of chronic disorders (ACD), beta thalassemia trait (β TT) and controls. Children withIDAand ACD were diagnosed on the basis of history and serum iron profile. Subjects with β TT had HbA2 > 3.5%. sTfR were performed on all subjects. Level of sTfR in patients withIDAwas 5.79 μg/ml ± 1.3 μg/ml. In patients with anaemia of chronic disorders (ACD), β thalassemia trait and controls mean sTfR were 2.18 μg/ml ± 0.6 μg/ml, 2.1μg/ml ± 0.5 μg/ml and 2.0 μg/ml ± 0.5 μg/ml respectively. These results show level of sTfR was raised in IDA when compared with controls or ACD and β TT (p
基金funded by the 863 Program entitled as"The research and exploration of nutrition fortified food for improving growth and development(2010AA023004)"performed by the Trace Elements Nutrition Key Laboratory of the Ministry of Health
文摘Objective To establish and evaluate a protein serum ferritin （SF） and soluble transferrin receptor microarray method for combined measurement of （sTfR）. Methods Microarrayer was used to print both anti-SF antibodies I and anti-sTfR antibodies I on each protein microarray. Anti-SF antibodies II and anti-sTfR antibodies II were used as detection antibodies and goat antibodies coupled to Cy3 were used as antibodies Ill. The detection conditions of the quantitative analysis method for simultaneous measurement of SF and sTfR with protein microarray were optimized and evaluated. The protein microarray was compared with commercially available traditional tests with 26 serum samples. Results By comparison experiment, mouse monoclonal antibodies were chosen as the probes and contact printing was chosen as the printing method. The concentrations of SF and sTfR probes were 0.5 mg/mL and 0.5 mg/mL respectively, while those of SF and sTfR detection antibodies were 5 μg/mL and 0.36 μg/mL respectively. Intra- and inter-assay variability was between 3.26% and 18.38% for all tests. The regression coefficients comparing protein microarray with traditional test assays were better than 0.81 for SF and sTfR. Conclusion The present study has established a protein microarray method for combined measurement of SF and sTfR.
文摘目的探究妊娠期贫血孕妇血清铁(serum iron,SI)及可溶性血清转铁蛋白受体(soluble serum transferrin receptor,sTfR)检测与妊娠结局的关系及临床意义。方法选取2020年1月至2021年6月同济大学附属第一妇婴保健院收治的1000例孕妇作为研究对象,根据有无贫血分为贫血组(n=195)和无贫血组(n=805),比较两组孕妇的一般资料、SI、sTfR水平。随访至妊娠结束,比较两组孕妇不良妊娠结局发生情况。比较不同妊娠结局贫血孕妇的SI、sTfR水平,采用受试者操作特征(receiver operating characteristic,ROC)曲线和曲线下面积(area under the curve,AUC)评估SI、sTfR水平对贫血孕妇妊娠结局的预测价值。统计学方法采用独立样本t检验和χ^(2)检验。结果妊娠期贫血的发生率为19.50%(195/1000)。贫血组孕妇SI水平低于无贫血组[(5.5±1.0)与(18.2±6.0)μmol/L,t=29.370,P<0.001];sTfR水平高于无贫血组[(3.8±1.3)与(1.9±0.6)mg/L,t=31.638,P<0.001]。贫血组孕妇不良妊娠结局总发生率高于无贫血组[23.59%(46/195)与4.10%(33/805),χ^(2)=81.957,P<0.05]。发生与未发生不良妊娠结局贫血孕妇的SI水平分别为[(4.6±0.8)与(5.7±0.8)μmol/L,t=15.366,P<0.001],发生较未发生不良妊娠结局贫血孕妇低;sTfR水平分别为[(4.6±1.2)与(3.6±1.0)mg/L,t=8.985,P<0.001],发生较未发生不良妊娠结局贫血孕妇高。SI、sTfR单独预测的AUC分别为0.743、0.770,联合预测的AUC最大,为0.924,最佳诊断敏感度、特异性分别为80.43%、87.92%。结论SI、sTfR水平变化与妊娠期贫血的发生密切相关,联合检测对于妊娠期贫血孕妇的妊娠结局具有较高预测价值,可作为临床早期评估预测的辅助指标。
文摘目的:观察世居高原中长跑运动员高住低练(living high training low,HiLo)期间血清EPO、sTfR及血象指标变化,探讨HiLo训练对世居高原耐力项目运动员红细胞生成的影响。方法:随机将20名世居海拔2260m高原的中长跑运动员(男12名,女8名)分成低氧组和对照组,每组10名(6男,4女)。低氧组在模拟海拔4000m低压低氧(大气压力为60kPa,氧气浓度为20.95%)环境下进行HiLo实验,每天低氧暴露10小时(8:00pm～6:00am),白天在其居住生活的海拔2260m高度进行训练;对照组睡、练均在海拔2260m环境。两组受试者实验期间的训练负荷、时间及营养状况均相同。实验进行4周。分别于实验前,实验开始1天、每周及实验结束后2周抽静脉血,测试血清促红细胞生成素(EPO)、血清转铁蛋白受体(sTfR)及血红蛋白(Hb)、红细胞压积(Hct)。结果:4周HiLo后,低氧组运动员Hb、Hct较实验前无明显改善(P>0.05),EPO除在低氧暴露第1天比实验前有升高趋势(P<0.10)外,整个实验期间与实验前及对照组相比均无显著性改变;HiLo训练后低氧组sTfR与实验前和对照组相比也未发生有统计意义的变化。结论:世居高原(海拔2260m)中长跑运动员进行4周HiLo训练对其EPO、sTfR、Hb升高作用不显著,表明世居高原中长跑运动员4周HiLo训练(模拟海拔4000m睡眠,海拔2260m训练)对机体红细胞生成影响不明显。
