[Objective] The paper was clear the effects of ferulic sesquiterpene compounds on enzyme activity of schistosomulum.[Method]Mice infected with schistosome cercariae were orally administrated with ferulic sesquiterpene...[Objective] The paper was clear the effects of ferulic sesquiterpene compounds on enzyme activity of schistosomulum.[Method]Mice infected with schistosome cercariae were orally administrated with ferulic sesquiterpene compounds at the dose of 300 mg/kg on the 6 thday post infection once a week for 4 weeks.Schistosomula were collected and ground into homogenate on the 3 rdand 5 thday after every time of administration.Thin layer starch gel electrophoresis was used to observe the activity of enzymes in the homogenate.Control group was also designed（drug free mice）.[Result] The activities of malic dehydrogenase,6-phophate mannosease and acid phosphatase were obviously inhibited in the drug group,but the activity of phosphoglucoisomerase was not inhibited.[Conclusion] Ferulic sesquiterpene compounds have inhibitory effect on energy metabolism of schistosomulum and digestion of red blood cells.展开更多
[Objectives]This study was conducted to explore the effects of ferulic sesquiterpene compounds on juvenile worms and adults of Schistosoma japonicum at different developmental stages in infected juvenile rabbits.[Meth...[Objectives]This study was conducted to explore the effects of ferulic sesquiterpene compounds on juvenile worms and adults of Schistosoma japonicum at different developmental stages in infected juvenile rabbits.[Methods]Juvenile rabbits were infected with S.japonicum quantitatively,and administered with 500 mg/kg ferulic sesquiterpene compounds at 2 h,1,2,6,11,13,15,24,36,and 41 d after infection by gavage,1 time/d,and a control group was administrated with normal saline.After 4 weeks of administration,the juvenile rabbits were dissected,and the worms were collected by portal vein perfusion for the calculation of worm reduction rate.[Results]The reduction rates of ferulic sesquiterpene compounds on 1,2,6,11,13,15,24,36,and 41-day-old juveniles or adults were 15.8%,17.0%,60.2%,50.2%,36.9%,31.3%,45.3%,57.0%,and 25.2%,respectively,and it was found that worms at the age of 6-36 d were most sensitive to the drug.[Conclusions]Ferulic sesquiterpene compounds had a killing effect on S.japonicum at different developmental stages in juvenile rabbits,and the killing effect on 6-36-day-old worms was more significant.展开更多
基金Supported by National Innovation Experiment Program for University Students in Yangtze River University(091048926)
文摘[Objective] The paper was clear the effects of ferulic sesquiterpene compounds on enzyme activity of schistosomulum.[Method]Mice infected with schistosome cercariae were orally administrated with ferulic sesquiterpene compounds at the dose of 300 mg/kg on the 6 thday post infection once a week for 4 weeks.Schistosomula were collected and ground into homogenate on the 3 rdand 5 thday after every time of administration.Thin layer starch gel electrophoresis was used to observe the activity of enzymes in the homogenate.Control group was also designed（drug free mice）.[Result] The activities of malic dehydrogenase,6-phophate mannosease and acid phosphatase were obviously inhibited in the drug group,but the activity of phosphoglucoisomerase was not inhibited.[Conclusion] Ferulic sesquiterpene compounds have inhibitory effect on energy metabolism of schistosomulum and digestion of red blood cells.
基金the Effects of Ferulic Sesquiterpene Esters on Active Enzymes of Schistosoma japonicum(No.KLPCAAB-2019-04).
文摘[Objectives]This study was conducted to explore the effects of ferulic sesquiterpene compounds on juvenile worms and adults of Schistosoma japonicum at different developmental stages in infected juvenile rabbits.[Methods]Juvenile rabbits were infected with S.japonicum quantitatively,and administered with 500 mg/kg ferulic sesquiterpene compounds at 2 h,1,2,6,11,13,15,24,36,and 41 d after infection by gavage,1 time/d,and a control group was administrated with normal saline.After 4 weeks of administration,the juvenile rabbits were dissected,and the worms were collected by portal vein perfusion for the calculation of worm reduction rate.[Results]The reduction rates of ferulic sesquiterpene compounds on 1,2,6,11,13,15,24,36,and 41-day-old juveniles or adults were 15.8%,17.0%,60.2%,50.2%,36.9%,31.3%,45.3%,57.0%,and 25.2%,respectively,and it was found that worms at the age of 6-36 d were most sensitive to the drug.[Conclusions]Ferulic sesquiterpene compounds had a killing effect on S.japonicum at different developmental stages in juvenile rabbits,and the killing effect on 6-36-day-old worms was more significant.
