BACKGROUND Diabetic intracerebral hemorrhage(ICH)is a serious complication of diabetes.The role and mechanism of bone marrow mesenchymal stem cell(BMSC)-derived exosomes(BMSC-exo)in neuroinflammation post-ICH in patie...BACKGROUND Diabetic intracerebral hemorrhage(ICH)is a serious complication of diabetes.The role and mechanism of bone marrow mesenchymal stem cell(BMSC)-derived exosomes(BMSC-exo)in neuroinflammation post-ICH in patients with diabetes are unknown.In this study,we investigated the regulation of BMSC-exo on hyperglycemia-induced neuroinflammation.AIM To study the mechanism of BMSC-exo on nerve function damage after diabetes complicated with cerebral hemorrhage.METHODS BMSC-exo were isolated from mouse BMSC media.This was followed by transfection with microRNA-129-5p(miR-129-5p).BMSC-exo or miR-129-5poverexpressing BMSC-exo were intravitreally injected into a diabetes mouse model with ICH for in vivo analyses and were cocultured with high glucoseaffected BV2 cells for in vitro analyses.The dual luciferase test and RNA immunoprecipitation test verified the targeted binding relationship between miR-129-5p and high-mobility group box 1(HMGB1).Quantitative polymerase chain reaction,western blotting,and enzyme-linked immunosorbent assay were conducted to assess the levels of some inflammation factors,such as HMGB1,interleukin 6,interleukin 1β,toll-like receptor 4,and tumor necrosis factorα.Brain water content,neural function deficit score,and Evans blue were used to measure the neural function of mice.RESULTS Our findings indicated that BMSC-exo can promote neuroinflammation and functional recovery.MicroRNA chip analysis of BMSC-exo identified miR-129-5p as the specific microRNA with a protective role in neuroinflammation.Overexpression of miR-129-5p in BMSC-exo reduced the inflammatory response and neurological impairment in comorbid diabetes and ICH cases.Furthermore,we found that miR-129-5p had a targeted binding relationship with HMGB1 mRNA.CONCLUSION We demonstrated that BMSC-exo can reduce the inflammatory response after ICH with diabetes,thereby improving the neurological function of the brain.展开更多
Objective Innate lymphoid cells(ILCs)are a class of newly discovered immunocytes.Group 1 ILCs(ILC1s)are identified in the decidua of humans and mice.High mobility group box 1(HMGB1)is predicted to be one of the target...Objective Innate lymphoid cells(ILCs)are a class of newly discovered immunocytes.Group 1 ILCs(ILC1s)are identified in the decidua of humans and mice.High mobility group box 1(HMGB1)is predicted to be one of the target genes of miR-142-3p,which is closely related to pregnancy-related diseases.Furthermore,miR-142-3p and HMGB1 are involved in regulating the NF-κB signaling pathway.This study aimed to examine the regulatory effect of miR-142-3p on ILC1s and the underlying mechanism involving HMGB1 and the NF-κB signaling pathway.Methods Mouse models of normal pregnancy and abortion were constructed,and the alterations of ILC1s,miR-142-3p,ILC1 transcription factor(T-bet),and pro-inflammatory cytokines of ILC1s(TNF-α,IFN-γand IL-2)were detected in mice from different groups.The targeting regulation of HMGB1 by miR-142-3p in ILC1s,and the expression of HMGB1 in normal pregnant mice and abortive mice were investigated.In addition,the regulatory effects of miR-142-3p and HMGB1 on ILC1s were detected in vitro by CCK-8,Annexin-V/PI,ELISA,and RT-PCR,respectively.Furthermore,changes of the NF-κB signaling pathway in ILC1s were examined in the different groups.For the in vivo studies,miR-142-3p-Agomir was injected in the uterus of abortive mice to evaluate the abortion rate and alterations of ILC1s at the maternal-fetal interface,and further detect the expression of HMGB1,pro-inflammatory cytokines,and the NF-κB signaling pathway.Results The number of ILC1s was significantly increased,the level of HMGB1 was significantly upregulated,and that of miR-142-3p was considerably downregulated in the abortive mice as compared with the normal pregnant mice(all P<0.05).In addition,miR-142-3p was found to drastically inhibit the activation of the NF-κB signaling pathway(P<0.05).The number of ILC1s and the levels of pro-inflammatory cytokines were significantly downregulated and the activation of the NF-κB signaling pathway was inhibited in the miR-142-3p Agomir group(all P<0.05).Conclusion miR-142-3p can regulate ILC1s by targeting HMGB1 via the NF-κB signaling pathway,and attenuate the inflammation at the maternal-fetal interface in abortive mice.展开更多
Digital broadcasting is a novel paradigm for the next generation broadcasting. Its goal is to provide not only better quality of pictures but also a variety of services that is impossible in traditional airwaves broad...Digital broadcasting is a novel paradigm for the next generation broadcasting. Its goal is to provide not only better quality of pictures but also a variety of services that is impossible in traditional airwaves broadcasting. One of the important factors for this new broadcasting environment is the interoperability among broadcasting applications since the environment is distributed. Therefore the broadcasting metadata becomes increasingly important and one of the metadata standards for a digital broadcasting is TV-Anytime metadata. TV-Anytime metadata is defined using XML schema, so its instances are XML data. In order to fulfill interoperability, a standard query language is also required and XQuery is a natural choice. There are some researches for dealing with broadcasting metadata. In our previous study, we have proposed the method for efficiently managing the broadcasting metadata in a service provider. However, the environment of a Set-Top Box for digital broadcasting is limited such as low-cost and low-setting. Therefore there are some considerations to apply general approaches for managing the metadata into the Set-Top Box. This paper proposes a method for efficiently managing the broadcasting metadata based on the Set-Top Box and a prototype of metadata management system for evaluating our method. Our system consists of a storage engine to store the metadata and an XQuery engine to search the stored metadata and uses special index for storing and searching. Our two engines are designed independently with hardware platform therefore these engines can be used in any low-cost applications to manage broadcasting metadata.展开更多
The conceptions of the knowledge screen generated by S-rough sets are given: f- screen and - screen , and then puts forward - filter theorem, - filter theorem of knowledge. At last, the applications of knowledge separ...The conceptions of the knowledge screen generated by S-rough sets are given: f- screen and - screen , and then puts forward - filter theorem, - filter theorem of knowledge. At last, the applications of knowledge separation are given according to - screen and - screen.展开更多
The geochemical characteristics of two sections—the Permian–Triassic boundary(PTB) Guryul Ravine section, Kashmir Valley, Jammu and Kashmir,India; and the Attargoo section, Spiti Valley, Himachal Pradesh, India—hav...The geochemical characteristics of two sections—the Permian–Triassic boundary(PTB) Guryul Ravine section, Kashmir Valley, Jammu and Kashmir,India; and the Attargoo section, Spiti Valley, Himachal Pradesh, India—have been studied in the context of provenance, paleo-weathering, and plate tectonic setting.These sections represent the siliciclastic sedimentary sequence from the Tethys Himalaya. The PTB siliciclastic sedimentary sequence in these regions primarily consists of sandstones and shales with variable thickness. Present studied sandstones and shales of both sections had chemical index of alteration values between 65 and 74; such values reveal low-to-moderate degree of chemical weathering. The chemical index of weathering in studied samples ranged from 71 to 94, suggesting a minor K-metasomatism effect on these samples. Plagioclase index of alteration in studied sections ranged from 68 to 92, indicating a moderate degree of weathering of plagioclase feldspars. The provenance discriminant function diagram suggests that the detritus involved in the formation of present studied siliciclastic sedimentary rocks fall in quartzose sedimentary and felsic igneous provenances. These sediments were deposited in a passive continental margin plate tectonic setting according to their location on a Si_2 O versus K_2O/Na_2 O tectonic setting diagram.展开更多
The FCSE controlling equation of pinned thinwalled curve box was derived and the indeterminate problem of continuous thin-walled curve box with diaphragm was solved based on flexibility theory. With Bayesian statistic...The FCSE controlling equation of pinned thinwalled curve box was derived and the indeterminate problem of continuous thin-walled curve box with diaphragm was solved based on flexibility theory. With Bayesian statistical theory,dynamic Bayesian error function of displacement parameters of indeterminate curve box was founded. The corresponding formulas of dynamic Bayesian expectation and variance were deduced. Combined with one-dimensional Fibonacci automatic search scheme of optimal step size,the Powell optimization theory was utilized to research the stochastic identification of displacement parameters of indeterminate thin-walled curve box. Then the identification steps were presented in detail and the corresponding calculation procedure was compiled. Through some classic examples,it is obtained that stochastic performances of systematic parameters and systematic responses are simultaneously deliberated in dynamic Bayesian error function. The one-dimensional optimization problem of the optimal step size is solved by adopting Fibonacci search method. And the Powell identification of displacement parameters of indeterminate thin-walled curve box has satisfied numerical stability and convergence,which demonstrates that the presented method and the compiled procedure are correct and reliable.During parameters鈥？iterative processes,the Powell theory is irrelevant with the calculation of finite curve strip element（FCSE） partial differentiation,which proves high computation effciency of the studied method.展开更多
基金Supported by the National Natural Science Foundation of China,No.81900743Heilongjiang Province Outstanding Young Medical Talents Training Grant Project,China,No.HYD2020YQ0007.
文摘BACKGROUND Diabetic intracerebral hemorrhage(ICH)is a serious complication of diabetes.The role and mechanism of bone marrow mesenchymal stem cell(BMSC)-derived exosomes(BMSC-exo)in neuroinflammation post-ICH in patients with diabetes are unknown.In this study,we investigated the regulation of BMSC-exo on hyperglycemia-induced neuroinflammation.AIM To study the mechanism of BMSC-exo on nerve function damage after diabetes complicated with cerebral hemorrhage.METHODS BMSC-exo were isolated from mouse BMSC media.This was followed by transfection with microRNA-129-5p(miR-129-5p).BMSC-exo or miR-129-5poverexpressing BMSC-exo were intravitreally injected into a diabetes mouse model with ICH for in vivo analyses and were cocultured with high glucoseaffected BV2 cells for in vitro analyses.The dual luciferase test and RNA immunoprecipitation test verified the targeted binding relationship between miR-129-5p and high-mobility group box 1(HMGB1).Quantitative polymerase chain reaction,western blotting,and enzyme-linked immunosorbent assay were conducted to assess the levels of some inflammation factors,such as HMGB1,interleukin 6,interleukin 1β,toll-like receptor 4,and tumor necrosis factorα.Brain water content,neural function deficit score,and Evans blue were used to measure the neural function of mice.RESULTS Our findings indicated that BMSC-exo can promote neuroinflammation and functional recovery.MicroRNA chip analysis of BMSC-exo identified miR-129-5p as the specific microRNA with a protective role in neuroinflammation.Overexpression of miR-129-5p in BMSC-exo reduced the inflammatory response and neurological impairment in comorbid diabetes and ICH cases.Furthermore,we found that miR-129-5p had a targeted binding relationship with HMGB1 mRNA.CONCLUSION We demonstrated that BMSC-exo can reduce the inflammatory response after ICH with diabetes,thereby improving the neurological function of the brain.
基金supported by the National Key Research and Development Program of China(Nos.2018YFC1002804 and 2016YFC1000600)the National Natural Science Foundation of China(Nos.81771618 and 81971356)the Fundamental Research Funds for the Central Universities(No.2042023kf0028).
文摘Objective Innate lymphoid cells(ILCs)are a class of newly discovered immunocytes.Group 1 ILCs(ILC1s)are identified in the decidua of humans and mice.High mobility group box 1(HMGB1)is predicted to be one of the target genes of miR-142-3p,which is closely related to pregnancy-related diseases.Furthermore,miR-142-3p and HMGB1 are involved in regulating the NF-κB signaling pathway.This study aimed to examine the regulatory effect of miR-142-3p on ILC1s and the underlying mechanism involving HMGB1 and the NF-κB signaling pathway.Methods Mouse models of normal pregnancy and abortion were constructed,and the alterations of ILC1s,miR-142-3p,ILC1 transcription factor(T-bet),and pro-inflammatory cytokines of ILC1s(TNF-α,IFN-γand IL-2)were detected in mice from different groups.The targeting regulation of HMGB1 by miR-142-3p in ILC1s,and the expression of HMGB1 in normal pregnant mice and abortive mice were investigated.In addition,the regulatory effects of miR-142-3p and HMGB1 on ILC1s were detected in vitro by CCK-8,Annexin-V/PI,ELISA,and RT-PCR,respectively.Furthermore,changes of the NF-κB signaling pathway in ILC1s were examined in the different groups.For the in vivo studies,miR-142-3p-Agomir was injected in the uterus of abortive mice to evaluate the abortion rate and alterations of ILC1s at the maternal-fetal interface,and further detect the expression of HMGB1,pro-inflammatory cytokines,and the NF-κB signaling pathway.Results The number of ILC1s was significantly increased,the level of HMGB1 was significantly upregulated,and that of miR-142-3p was considerably downregulated in the abortive mice as compared with the normal pregnant mice(all P<0.05).In addition,miR-142-3p was found to drastically inhibit the activation of the NF-κB signaling pathway(P<0.05).The number of ILC1s and the levels of pro-inflammatory cytokines were significantly downregulated and the activation of the NF-κB signaling pathway was inhibited in the miR-142-3p Agomir group(all P<0.05).Conclusion miR-142-3p can regulate ILC1s by targeting HMGB1 via the NF-κB signaling pathway,and attenuate the inflammation at the maternal-fetal interface in abortive mice.
文摘Digital broadcasting is a novel paradigm for the next generation broadcasting. Its goal is to provide not only better quality of pictures but also a variety of services that is impossible in traditional airwaves broadcasting. One of the important factors for this new broadcasting environment is the interoperability among broadcasting applications since the environment is distributed. Therefore the broadcasting metadata becomes increasingly important and one of the metadata standards for a digital broadcasting is TV-Anytime metadata. TV-Anytime metadata is defined using XML schema, so its instances are XML data. In order to fulfill interoperability, a standard query language is also required and XQuery is a natural choice. There are some researches for dealing with broadcasting metadata. In our previous study, we have proposed the method for efficiently managing the broadcasting metadata in a service provider. However, the environment of a Set-Top Box for digital broadcasting is limited such as low-cost and low-setting. Therefore there are some considerations to apply general approaches for managing the metadata into the Set-Top Box. This paper proposes a method for efficiently managing the broadcasting metadata based on the Set-Top Box and a prototype of metadata management system for evaluating our method. Our system consists of a storage engine to store the metadata and an XQuery engine to search the stored metadata and uses special index for storing and searching. Our two engines are designed independently with hardware platform therefore these engines can be used in any low-cost applications to manage broadcasting metadata.
文摘The conceptions of the knowledge screen generated by S-rough sets are given: f- screen and - screen , and then puts forward - filter theorem, - filter theorem of knowledge. At last, the applications of knowledge separation are given according to - screen and - screen.
文摘The geochemical characteristics of two sections—the Permian–Triassic boundary(PTB) Guryul Ravine section, Kashmir Valley, Jammu and Kashmir,India; and the Attargoo section, Spiti Valley, Himachal Pradesh, India—have been studied in the context of provenance, paleo-weathering, and plate tectonic setting.These sections represent the siliciclastic sedimentary sequence from the Tethys Himalaya. The PTB siliciclastic sedimentary sequence in these regions primarily consists of sandstones and shales with variable thickness. Present studied sandstones and shales of both sections had chemical index of alteration values between 65 and 74; such values reveal low-to-moderate degree of chemical weathering. The chemical index of weathering in studied samples ranged from 71 to 94, suggesting a minor K-metasomatism effect on these samples. Plagioclase index of alteration in studied sections ranged from 68 to 92, indicating a moderate degree of weathering of plagioclase feldspars. The provenance discriminant function diagram suggests that the detritus involved in the formation of present studied siliciclastic sedimentary rocks fall in quartzose sedimentary and felsic igneous provenances. These sediments were deposited in a passive continental margin plate tectonic setting according to their location on a Si_2 O versus K_2O/Na_2 O tectonic setting diagram.
基金supported by the National Natural Science Foundation of China (10472045, 10772078 and 11072108)the Science Foundation of NUAA(S0851-013)
文摘The FCSE controlling equation of pinned thinwalled curve box was derived and the indeterminate problem of continuous thin-walled curve box with diaphragm was solved based on flexibility theory. With Bayesian statistical theory,dynamic Bayesian error function of displacement parameters of indeterminate curve box was founded. The corresponding formulas of dynamic Bayesian expectation and variance were deduced. Combined with one-dimensional Fibonacci automatic search scheme of optimal step size,the Powell optimization theory was utilized to research the stochastic identification of displacement parameters of indeterminate thin-walled curve box. Then the identification steps were presented in detail and the corresponding calculation procedure was compiled. Through some classic examples,it is obtained that stochastic performances of systematic parameters and systematic responses are simultaneously deliberated in dynamic Bayesian error function. The one-dimensional optimization problem of the optimal step size is solved by adopting Fibonacci search method. And the Powell identification of displacement parameters of indeterminate thin-walled curve box has satisfied numerical stability and convergence,which demonstrates that the presented method and the compiled procedure are correct and reliable.During parameters鈥？iterative processes,the Powell theory is irrelevant with the calculation of finite curve strip element（FCSE） partial differentiation,which proves high computation effciency of the studied method.
