Magnetic refrigeration is a revolutionary, efficient, environmentally friendly cooling technology, which is on the threshold of commercialization. The magnetic rare earth materials are utilized as the magnetic refrige...Magnetic refrigeration is a revolutionary, efficient, environmentally friendly cooling technology, which is on the threshold of commercialization. The magnetic rare earth materials are utilized as the magnetic refrigerants in most cooling devices, and for many cooling application the Nd2Fe14B permanent magnets are employed as the source of the magnetic field. The status of the near room temperature magnetic cooling was reviewed.展开更多
Green-revertible albino mutants are important sources for studying chloroplast structure, chloroplast development, chlorophyll biosynthesis, and plant photo- synthesis. In the present study, we characterized a green- ...Green-revertible albino mutants are important sources for studying chloroplast structure, chloroplast development, chlorophyll biosynthesis, and plant photo- synthesis. In the present study, we characterized a green- revertible albino mutant gra（k）, which was obtained from the tissue-cultured rice Kitaake. The mutant gra（k） exhib- ited albino on its first three leaves. The leaf color started to turn green at the four-leaf stage. The chlorophyll contents were deeply reduced at the seedling stage, and the chloroplast development was delayed in gra（k）. The green- revertible albino （gra） phenotype of the mutant gra（k） was temperature dependent. The main agronomic traits, including plant height, tilling number per plant, seed set- ting rate, and thousand-grain weight, slightly decreased in gra（k） comparing to those in the wild-type Kitaake. Genetic analysis showed that the gra phenotype was con- trolled by a single recessive nucleic gene. By using 5,168 recessive F2 individuals derived from the cross of gra（k） × Jodan, the locus of the gene Gra（k） was delimited in a DNA region of 200 kb between the makers B-31 and P11 on chromosome 5. Sequencing analysis indicated that the three functionally annotated genes, LOC_Os05g23700, LOC_Os05g23720, and LOC_Os05g23740, were all deleted in the 200 kb region in the mutant gra（k）. Trans- genic test revealed that the gra（k） plants over-expressing LOC_Os05g23740CDS were restored to normal green as the wild-type Kitaake. Our results proved that the deletion of the DnaK protein gene LOC_Os05g23740 （encoding the chaperon protein OsHsp70CP1） led to the gra phenotype in the mutant gra（k）.展开更多
基金Project supported bythe U.S .Department of Energy ,Office of Basic Energy Sciences , Materials Science and Engineering Division and Astronautics Corporation of America , Milwaukee , Wisconsin
文摘Magnetic refrigeration is a revolutionary, efficient, environmentally friendly cooling technology, which is on the threshold of commercialization. The magnetic rare earth materials are utilized as the magnetic refrigerants in most cooling devices, and for many cooling application the Nd2Fe14B permanent magnets are employed as the source of the magnetic field. The status of the near room temperature magnetic cooling was reviewed.
基金supported by the National Natural Science Foundation of China(3117162231371705+5 种基金314013531501627)the‘‘Hundred Talents Plan’’Foundation of Sichuanand the Specialized Research Funds for Doctoral Program of Higher Education(2012510312001120135103120004)the Key Project of Sichuan Education Department(15ZA0020)
文摘Green-revertible albino mutants are important sources for studying chloroplast structure, chloroplast development, chlorophyll biosynthesis, and plant photo- synthesis. In the present study, we characterized a green- revertible albino mutant gra（k）, which was obtained from the tissue-cultured rice Kitaake. The mutant gra（k） exhib- ited albino on its first three leaves. The leaf color started to turn green at the four-leaf stage. The chlorophyll contents were deeply reduced at the seedling stage, and the chloroplast development was delayed in gra（k）. The green- revertible albino （gra） phenotype of the mutant gra（k） was temperature dependent. The main agronomic traits, including plant height, tilling number per plant, seed set- ting rate, and thousand-grain weight, slightly decreased in gra（k） comparing to those in the wild-type Kitaake. Genetic analysis showed that the gra phenotype was con- trolled by a single recessive nucleic gene. By using 5,168 recessive F2 individuals derived from the cross of gra（k） × Jodan, the locus of the gene Gra（k） was delimited in a DNA region of 200 kb between the makers B-31 and P11 on chromosome 5. Sequencing analysis indicated that the three functionally annotated genes, LOC_Os05g23700, LOC_Os05g23720, and LOC_Os05g23740, were all deleted in the 200 kb region in the mutant gra（k）. Trans- genic test revealed that the gra（k） plants over-expressing LOC_Os05g23740CDS were restored to normal green as the wild-type Kitaake. Our results proved that the deletion of the DnaK protein gene LOC_Os05g23740 （encoding the chaperon protein OsHsp70CP1） led to the gra phenotype in the mutant gra（k）.
