Spatial deduction of mining-induced stress redistribution using an optimized non-negative matrix factorization model

Investigation of mining-induced stress is essential for the safety of coal production.Although the field monitoring and numerical simulation play a significant role in obtaining the structural mechanical behaviors,the range of monitoring is not sufficient due to the limits of monitoring points and the associated numerical result is not accurate.In this study,we aim to present a spatial deduction model to characterize the mining-induced stress distribution using machine learning algorithm on limited monitoring data.First,the framework of the spatial deduction model is developed on the basis of non-negative matrix factorization(NMF)algorithm and optimized by mechanical mechanism.In this framework,the spatial correlation of stress response is captured from numerical results,and the learned correlation is employed in NMF as a mechanical constrain to augment the limited monitoring data and obtain the overall mechanical performances.Then,the developed model is applied to a coal mine in Shandong,China.Experimental results show the stress distribution in one plane is derived by several monitoring points,where mining induced stress release is observed in goaf and stress concentration in coal pillar,and the intersection point between goaf and coal seam is a sensitive area.The indicators used to evaluate the property of the presented model indicate that 83%mechanical performances have been captured and the deduction accuracy is about 92.9%.Therefore,it is likely that the presented deduction model is reliable.

Deformation characteristics and reinforcement technology for entry subjected to mining-induced stresses

The entry at Zhangcun coal mine in Lu＇an coal mining area in Shanxi Province suffered from severe mining-induced stresses with the heading face driven oppositely to an adjacent working face. In this paper, the characteristics of deformation and failure of the entry were investigated in terms of the tempo-spatial relations between heading and working faces through field study and numerical modeling. The three-dimensional （3D） finite difference models were built to investigate stresses, displacements and damages in the surrounding rocks of the entry and the working face. The field study includes selection of reinforcing methods and materials, design parameters, and determination of cable prestress. The monitoring data of entry deformation and stress along the cables during every stage were presented. The state of the reinforced entry was evaluated based on the monitoring data. The results demonstrate that before the heading face of the entry crosses the adjacent working face, the influence of advanced abutment pressure caused by adjacent working face upon the entry is not significant. After they cross each other, however, the lateral abutment pressure will have an evident impact on the entry. The displacement rate of the entry will be greatly increased and reaches a certain value within a certain distance between the heading face and the working face. Then, it will increase again with the presence of secondary mining-induced pressure on the entry when the present working face advances. The fully-grouted cable with short length, high strength and high prestress is an effective way to reinforce the entry suffering from severe mining-induced stresses, which greatly reduces the displacement and failure possibility of the entry. Finally, the principles and recommendations for reinforcing design of entries suffering from severe mining-induced stresses were proposed according to field study, numerical modeling and experiences from other coal mines. Problems encountered in field study and suggestions for reinforcement were also discussed.

Fault-Induced Coal Burst Mechanism under Mining-Induced Static and Dynamic Stresses

Fault is a common geological structure that has been revealed in the process of underground coal excavation and mining.The nature of its discontinuous structure controls the deformation,damage,and mechanics of the coal or rock mass.The interaction between this discontinuous structure and mining activities is a key factor that dominates fault reactivation and the coal burst it can induce.This paper first summarizes investigations into the relationships between coal mining layouts and fault occurrences,along with relevant conceptual models for fault reactivation.Subsequently,it proposes mechanisms of fault reactivation and its induced coal burst based on the superposition of static and dynamic stresses,which include two kinds of fault reactivations from:mining-induced quasi-static stress(FRMSS)-dominated and seismic-based dynamic stress(FRSDS)-dominated.These two kinds of fault reactivations are then validated by the results of experimental investigations,numerical modeling,and in situ microseismic monitoring.On this basis,monitoring methods and prevention strategies for fault-induced coal burst are discussed and recommended.The results show that fault-induced coal burst is triggered by the superposition of high static stress in the fault pillar and dynamic stress from fault reactivation.High static stress comes from the interaction of the fault and the roof structure,and dynamic stress can be ascribed to FRMSS and FRSDS.The results in this paper could be of great significance in guiding the monitoring and prevention of fault-induced coal bursts.

Abdominal paracentesis drainage ameliorates myocardial injury in severe experimental pancreatitis rats through suppressing oxidative stress

BACKGROUND Abdominal paracentesis drainage(APD)is a safe and effective strategy for severe acute pancreatitis(SAP)patients.However,the effects of APD treatment on SAPassociated cardiac injury remain unknown.AIM To investigate the protective effects of APD on SAP-associated cardiac injury and the underlying mechanisms.METHODS SAP was induced by 5%sodium taurocholate retrograde injection in Sprague-Dawley rats.APD was performed by inserting a drainage tube with a vacuum ball into the lower right abdomen of the rats immediately after SAP induction.Morphological staining,serum amylase and inflammatory mediators,serum and ascites high mobility group box(HMGB)1,cardiac-related enzymes indexes and cardiac function,oxidative stress markers and apoptosis and associated proteins were assessed in the myocardium in SAP rats.Nicotinamide adenine dinucleotide phosphate oxidase activity and mRNA and protein expression were also examined.RESULTS APD treatment improved cardiac morphological changes,inhibited cardiac dysfunction,decreased cardiac enzymes and reduced cardiomyocyte apoptosis,proapoptotic Bax and cleaved caspase-3 protein levels.APD significantly decreased serum levels of HMGB1,inhibited nicotinamide adenine dinucleotide phosphate oxidase expression and ultimately alleviated cardiac oxidative injury.Furthermore,the activation of cardiac nicotinamide adenine dinucleotide phosphate oxidase by pancreatitis-associated ascitic fluid intraperitoneal injection was effectively inhibited by adding anti-HMGB1 neutralizing antibody in rats with mild acute pancreatitis.CONCLUSION APD treatment could exert cardioprotective effects on SAP-associated cardiac injury through suppressing HMGB1-mediated oxidative stress,which may be a novel mechanism behind the effectiveness of APD on SAP.

Characteristics of evolution of mining-induced stress field in the longwall panel:insights from physical modeling

The evolution of mining-induced stress field in longwall panel is closely related to the fracture field and the breaking characteristics of strata.Few laboratory experiments have been conducted to investigate the stress field.This study investigated its evolution by constructing a large-scale physical model according to the in situ conditions of the longwall panel.Theoretical analysis was used to reveal the mechanism of stress distribution in the overburden.The modelling results showed that:(1)The major principal stress field is arch-shaped,and the strata overlying both the solid zones and gob constitute a series of coordinated load-bearing structures.The stress increasing zone is like a macro stress arch.High stress is especially concentrated on both shoulders of the arch-shaped structure.The stress concentration of the solid zone in front of the gob is higher than the rear solid zone.(2)The characteristics of the vertical stress field in different regions are significantly different.Stress decreases in the zone above the gob and increases in solid zones on both sides of it.The mechanical analysis show that for a given stratum,the trajectories of principal stress are arch-shaped or inverselyarched,referred to as the‘‘principal stress arch’’,irrespective of its initial breaking or periodic breaking,and determines the fracture morphology.That is,the trajectories of tensile principal stress are inversely arched before the first breaking of the strata,and cause the breaking lines to resemble an inverted funnel.In case of periodic breaking,the breaking line forms an obtuse angle with the advancing direction of the panel.Good agreement was obtained between the results of physical modeling and the theoretical analysis.

Numerical simulation of spatial distributions of mining-induced stress and fracture fields for three coal mining layouts

In this study, the spatial distributions of stress and fracture fields for three typical underground coal mining layouts, Le, non-pillar mining （NM）, top-coal caving mining （TCM） and protective coal-seam mining （PCM）, are modeled using discrete element software UDEC, The numerical results show that different mining layouts can lead to different mining-induced stress fields, resulting in diverse fracture fields, For the PCM, the mining influenced area in front of the mining faces is the largest, and the stress concentration factor in front of the mining faces is the lowest, The spatial shapes of the mining-induced fracture fields under NM, TCM and PCM differ, and they are characterized by trapezoidal, triangular and tower shapes, respectively, The fractal dimensions of mining-induced fractures of the three mining layouts decrease in the order of PCM, TCM and NM, It is also shown that the PCM can result in a better gas control effect in coal mines with high outburst potential, The numerical results are expected to provide a basis for understanding of mining-induced gas seepage fields and provide a reference for high- efficiency coal mining,

Development of a time-dependent energy model to calculate the mining-induced stress over gates and pillars

Generally, longwall mining-induced stress results from the stress relaxation due to destressed zone that occurs above the mined panel. Knowledge of induced stress is very important for accurate design of adjacent gateroads and intervening pillars which helps to raise the safety and productivity of longwall mining operations. This study presents a novel time-dependent analytical model for determination of the longwall mining-induced stress and investigates the coefficient of stress concentration over adjacent gates and pillars. The model is developed based on the strain energy balance in longwall mining incorporated to a rheological constitutive model of caved materials with time-varying parameters. The study site is the Tabas coal mine of Iran. In the proposed model, height of destressed zone above the mined panel, total longwall mining-induced stress, abutment angle, induced vertical stress, and coefficient of stress concentration over neighboring gates and intervening pillars are calculated. To evaluate the effect of proposed model parameters on the coefficient of stress concentration due to longwall mining, sensitivity analysis is performed based on the field data and experimental constants. Also, the results of the proposed model are compared with those of existing models. The comparative results confirm a good agreement between the proposed model and the in situ measurements. According to the obtained results, it is concluded that the proposed model can be successfully used to calculate the longwall mining-induced stress. Therefore, the optimum design of gate supports and pillar dimensions would be attainable which helps to increase the mining efficiency.

Effects of Severe Drought and Glyphosate Stress on Physiological Characteristics and Protein Expression of Photosystem Ⅱ in Genentically Modified Soybean

[Objective] The paper was to investigate effects of glyphosate stress on physiological characteristics and protein expression of photosystem Ⅱ(PSⅡ) in genentically modified soybean GTS 40-3-2 seedlings under severe drought condition. [Method] A pot experiment was carried out in growth chamber to determine the response of genetically modified soybean treated by severe drought stress and different concentrations of glyphosate at the third compound leaf stage. [Result] Severe drought treatment increased the electrolyte leakage(EL), superoxide dismutase(SOD) and peroxidase(POD) activities, and decreased the relative water content(RWC), chlorophyll content, and catalase(CAT) activity. The EL, SOD and POD activities were significantly increased in severe drought and glyphosate treatments, which were related to glyphosate concentrations. The chlorophyll content decreased, which was also related to glyphosate concentrations. But the BWC and CAT activity were not affected by glyphosate concentrations. Western blot displayed that PSⅡ protein Lhcb2 was not affected by stress conditions and stably expressed. D1, D2 and Lhcb4 protein level decreased, and there was no significant change in Lhcb1 expression under severe drought stress. The protein levels of D1, D2, Lhcb1 and Lhcb4 decreased with the increase of glyphosate concentrations under severe drought and glyphosate stress. When the glyphosate concentrations were 0.92 and 1.84 kg·ai/hm^2, the protein levels of D1, D2 and Lhcb4 were slightly higher than those in severe drought stress. When the glyphosate concentrations were 3.68 and 7.36 kg·ai/hm^2, the protein level of D1, D2, Lhcb1 and Lhcb4 decreased sharply. [Conclusion] This research provides a theoretical basis for production of genetically modified soybean.

Effects of ulinastatin + danshen + somatostatin on inflammation and oxidative stress in patients with severe pancreatitis

Objective: To explore the effects of ulinastatin + danshen + somatostatin on inflammation and oxidative stress in patients with severe pancreatitis. Methods: A total of 72 patients with severe acute pancreatitis who were treated in this hospital between September 2014 and December 2016 were divided into control group (n=36) and study group (n=36) by random number table method, control group received conventional treatment combined with danshen + somatostatin therapy, and study group received conventional treatment combined with ulinastatin + danshen+ somatostatin therapy. The differences in the levels of systemic inflammation and oxidative stress indexes were compared between the two groups before and after treatment. Results:Immediately after admission, the differences in the serum levels of inflammatory mediators and oxidative stress indexes were not statistically significant between the two groups. After 10 d of treatment, inflammatory mediators IL-1β, IL-6, sICAM-1 and PCT levels in the serum samples of study group were lower than those of control group;oxidation indexes MDA, LHP and AOPPs levels in serum samples of study group were lower than those of control group whereas anti-oxidation indexes SOD, GSH-Px and CAT levels were higher than those of control group. Conclusion: Ulinastatin + danshen + somatostatin therapy can effectively inhibit the inflammation and oxidative stress in patients with severe pancreatitis.

Effects of mucosolvan combined with fiberoptic bronchoscopy on respiratory function, inflammatory response and stress state in patients with severe pneumonia

Objective: To investigate the effect of mucosolvan combined with fiberoptic bronchoscopy on respiratory function, inflammatory response and stress state in patients with severe pneumonia. Methods: From January 2017 to June 2018, 82 patients with severe pneumonia were randomly divided into observation group and control group (all 41 cases). Patients in the control group received conventional anti-infective treatment, and the observation group was treated with fiberoptic bronchoscopy combined with mucosolvan on the basis of the control group. Respiratory function, inflammatory response and stress status were compared between the two groups. Results: Before treatment, there was no significant difference in Cdyn, WOB and PaO2/FiO2 between the two groups. After treatment, Cdyn and PaO2/FiO2 in the observation group were (36.28±4.28) mL/cmH2O and (376.23±24.21) mmHg respectively, while those in the control group were (26.89±3.76) mL/cmH2O and (322.12±23.16) mmHg, respectively. The levels of Cdyn and PaO2/FiO2 in the observation group were higher than those in the control group. After treatment, the WOB in the observation group was (7.81±0.72) J/L, and the WOB in the control group was (8.33±1.23) J/L. WOB of both groups was lower than that before treatment, and in observation group WOB was lower than that of control group, the difference was statistically significant. In CRP, PCT and sTREM-1 levels, there was no significant difference between the two groups before treatment. After treatment, CRP, PCT and sTREM-1 in the observation group were (39.10±6.03) mg/L, (14.57±2.05) ng/L, (15.02±3.02) ng/L respectively, while those in the control group were (59.72±8.81) mg/L, (20.03±3.09) ng/L, (34.21±5.28) ng/L, respectively. CRP, PCT, sTREM-1 in both groups were lower than those before treatment, and CRP, PCT, sTREM-1 in observation group were lower than those in control group. Before treatment, there was no significant difference with Cor, Ang-I and Ang-II in two groups. After treatment, the levels of Cor, Ang-I and Ang-II in the observation group were (114.76±15.85) ng/mL, (6.72±0.64) ng/mL, (27.28±3.43) ng/mL respectively, while those in the control group were (193.15±22.64) ng/mL, (12.10±1.68) ng/mL, (43.02±5.57) ng/mL, respectively. In the observation group, the levels of Cor, Ang-I and Ang-II were lower than those in the control group. Conclusion: Mucosolvan combined with fiberoptic bronchoscopy can effectively improve the respiratory function of patients with severe pneumonia, and reduce inflammation and stress state of the body.

Effects of short-term intensive insulin therapy on systemic inflammatory response and stress response in patients with severe pneumonia

Objective:To study the effects of short-term intensive insulin therapy on systemic inflammatory response and stress response in patients with severe pneumonia.Methods:Patients with severe pneumonia who were treated in our hospital between March 2015 and October 2017 were chosen as the research subjects and randomly divided into the INS group who received short-term intensive insulin combined with routine therapy and the control group who received short-acting insulin combined with routine therapy. The levels of inflammatory factors and stress mediators in serum as well as the expression intensity of inflammatory molecules in peripheral blood were determined before treatment as well as 3 d and 7 d after treatment.Results:Compared with same group before treatment, serum IL-1β, IL-13, suPAR, sTREM1, sP-selectin, SF, 8-iso-PGF2α, AQP1 and AQP5 levels as well as peripheral blood NF-κB, COX2, RANTES, CD28 and CD80 expression intensity of both groups significantly decreased 3 days and 7 days after treatment, and serum IL-1β, IL-13, suPAR, sTREM1, sP-selectin, SF, 8-iso-PGF2α, AQP1 and AQP5 levels as well as peripheral blood NF-κB, COX2, RANTES, CD28 and CD80 expression intensity of INS group 3 d and 7 d after treatment were significantly lower than those of control group.Conclusion: Short-term intensive insulin therapy has inhibitory effect on the systemic inflammatory response and stress response in patients with severe pneumonia.

Correlation of intestinal flora disorder with systemic inflammatory response and stress response in children with severe pneumonia

Objective:To study the correlation of intestinal flora disorder with systemic inflammatory response and stress response in children with severe pneumonia.Methods: The children who were diagnosed with severe pneumonia in Xiangyang No. 1 People's Hospital between April 2014 and December 2017 were selected as the pneumonia group of the study, and the healthy children who received physical examination in Xiangyang No. 1 People's Hospital during the same period were selected as the control group. The feces was collected to determine the number of intestinal flora bifidobacteria and Escherichia coli (E. coli). Besides, the serum was collected to determine the contents of inflammatory cytokines and oxidative stress indexes, and the peripheral blood was collected to determine the expression intensity of inflammatory molecules and oxidative stress molecules.Results:The number of bifidobacteria and the level of Bifidobacterium and E. coli ratio B/E in feces as well as SOD content in serum of pneumonia group were significantly lower than those of control group whereas the number of E. coli in feces, TLR2, TLR4, NOX2, iNOS and FOXP3 expression intensity in peripheral blood as well as G-CSF, sTREM1, TNF-α, LPO and NO contents in serum were significantly higher than those of control group;Pearson correlation analysis showed that B/E level in feces of pneumonia group was negatively correlated with TLR2, TLR4, NOX2, iNOS and FOXP3 expression intensity in peripheral blood as well as G-CSF, sTREM1, TNF-α, LPO and NO contents in serum, and positively correlated with SOD content in serum.Conclusion:The intestinal flora disorder in children with severe pneumonia can aggravate the degree of systemic inflammatory response and stress response in the course of disease.

Effect of ulinastatin combined with antibiotics on systemic inflammatory response and stress hormone secretion in patients with acute severe pneumonia

Objective:To study the effect of ulinastatin combined with antibiotics on systemic inflammatory response and stress hormone secretion in patients with acute severe pneumonia. Methods: A total of 78 patients with acute severe pneumonia in our hospital between December 2014 and January 2017 were randomly divided into routine group and ulinastatin group, each with 39 cases. Routine group were treated with conventional antibiotics, ulinastatin group were treated with ulinastatin and antibiotics, and the treatments lasted for 2 weeks. The differences of serum acute phase proteins, inflammatory factors and stress hormones were compared between the two groups before and after treatment.Results: Before treatment, there were no significant differences in serum contents of acute phase proteins, inflammatory factors or stress hormones between the two groups. After 2 weeks of treatment, serum acute phase protein prealbumin (PA) content in ulinastatin group was higher than that in routine group whereas C-reactive protein (CRP) content was lower than that in routine group;serum inflammatory cytokines interleukin-1β (IL-1β), interleukin-2 (IL-2) and interleukin-6 (IL-6) contents in ulinastatin group were lower than those in routine group;serum stress hormones NE, Cor and AngII contents in ulinastatin group were lower than those in routine group. Conclusion:Ulinastatin combined with antibiotics for acute severe pneumonia can inhibit the systemic inflammatory response and stress response.

Effects of ulinastatin combined with thymopentin on cellular immunity, humoral immunity and stress response in severe pneumonia

Objective:To explore the effects of ulinastatin combined with thymopentin on cellular immunity, humoral immunity and stress response in severe pneumonia.Methods: A total of 102 cases of severe pneumonia treated in our hospital from February 2016 to November 2017 were collected as subjects and randomly divided into the control group (n=51) and the observation group (n=51), the two groups were treated with routine symptomatic treatment. The control group was treated with the ulinastatin on the basis of routine treatment, the observation group was treated with thymopentin on the basis of the control group. The changes of cellular immunity, humoral immunity, stress response and liver function in the two groups were compared.Results: Before treatment, there was no significant difference in the levels of CD4+, CD8+, CD4+/CD8+, IgA, IgM, IgG, SOD, MDA, T-AOC, AKP, TB and ALT between the two groups (P>0.05). After treatment, the two groups of CD4+ and CD4+ /CD8+ were significantly increased (P<0.05), CD8+ was significantly lower than before treatment (P<0.05), and CD4+ and CD4+ /CD8+ in the observation group were significantly increased compared with the control group (P<0.05), CD8+was significantly lower than the control group (P<0.05);the two groups of IgA, IgM and IgG were significantly increased compared with those before treatment (P<0.05), and the IgA, IgM and IgG in the observation group were significantly higher than those in the control group (P<0.05);two groups of SOD and T-AOC were significantly higher than before treatment (P<0.05), while MDA was significantly lower than before treatment (P<0.05), and SOD and T-AOC in the observation group were significantly increased (P<0.05), and MDA was significantly lower than that of the control group (P<0.05);two groups of AKP, TB and ALT were significantly lower than those before treatment (P<0.05), and the AKP, TB and ALT in the observation group were significantly lower than those in the control group (P<0.05).Conclusions: ulinastatin combined with thymopentin in patients with severe pneumonia can effectively enhance the cellular immunity and humoral immune function, reduce oxidative stress damage and protect the liver function, which has clinical significance.

Effect of bifidobacterium enteral nutrition on intestinal flora and systemic inflammatory stress in patients with severe stroke

Objective:To study the effect of bifidobacterium enteral nutrition on intestinal flora and systemic inflammatory stress in patients with severe stroke.Methods: Patients with severe stroke who were treated in our hospital between March 2015 and July 2017 were selected and randomly divided into the observation group who accepted bifidobacterium enteral nutrition and the control group who accepted conventional enteral nutrition, and the contents of intestinal flora in feces as well as the contents of intestinal mucosal barrier markers, inflammatory response indexes, stress response indexes in serum were determined.Results: D-lactic acid, DAO, PCT, Claudin-4, TNF-α, IL-1β, Hcy, sICAM1, NE, Cor and MDA contents in serum of both groups 14 days after treatment were lower than those before treatment whereas T-AOC contents were higher than those before treatment, and bifidobacterium, lactobacillus and bacteroides contents in feces as well as T-AOC content in serum of observation group 14 d after treatment were higher than those of control group whereas enterococcus and enterobacter contents in feces as well as D-lactic acid, DAO, PCT, Claudin-4, TNF-α, IL-1β, Hcy, sICAM1, NE, Cor and MDA contents in serum were lower than those of control group. Conclusion: Bifidobacterium enteral nutrition is able to improve the intestinal flora and inhibit the systemic inflammatory stress in patients with severe stroke.

Effect of continuous low effective dialysis combined with hemoperfusion on inflammatory stress, hemodynamic parameters and renal function in septic patients with severe acute kidney injury

Objective:To investigate the effects of sustained low-efficiency dialysis (SLED) combined with hemoperfusion (HP) on inflammatory stress, hemodynamic parameters and renal function in septic patients with severe acute kidney injury.Methods: A total of 82 cases of sepsis with severe acute renal injury were divided into control group (n=41) and observation group (n=41) according to the random data table method. Two groups of patients were given conventional support treatment, on this basis, the control group was treated with Continuous renal replacement therapy (CRRT), and the observation group was treated with SLED plus HP. The levels of inflammatory factors, hemorheological parameters and renal function indexes of the two groups were compared before and after treatment.Results: There was no significant difference in the levels of CRP, WBC, PCT, MAP, OI, BUN and Scr between the control group and the observation group before the treatment. Compared with the level before treatment, the levels of CRP, WBC, PCT, BUN and Scr in the two groups were significantly decreased after treatment, while the levels of MAP and OI were significantly increased. Compared with the level of the control group after treatment, the levels of CRP, WBC, PCT, BUN and Scr in the observation group were significantly lower than those in the control group, the levels of MAP and OI were significantly increased, the difference was statistically significant.Conclusion:SLED combined with HP regimen in treatment of sepsis combined with severe acute kidney injury can effectively inhibit the release of inflammatory factors, improve hemodynamics and renal function, and has an important clinical value.

Effects of intensive insulin therapy on immune function, inflammatory markers, matrix metalloproteinase and stress response in patients with severe pneumonia

Objective:To explore the effects of intensive insulin therapy on immune function, inflammatory markers, matrix metalloproteinase and stress response in patients with severe pneumonia.Methods: From January 2016 to December 2017, 80 cases of severe pneumonia in ICU of our hospital were selected as the subjects of this study, according to the principle of randomization, they were divided into control group (40 cases) and observation group (40 cases). The control group was given routine insulin + routine treatment, and the observation group was given insulin intensification + routine treatment. The changes of immune function, inflammation index, matrix metalloproteinase and stress level were compared between the two groups before and after treatment.Results: After treatment, the levels of IgA, IgG and IgM in the two groups were significantly higher than those before treatment, and the levels of IgA, IgG and IgM in the observation group were significantly higher than those in the control group;the levels of TNF-α, sTREM1 and CRP in the two groups were significantly lower than those before treatment, and the levels of TNF-α, sTREM1 and CRP in the observation group were significantly lower than those in the control group;the levels of TIMP-1, MMP-9 and MMP-9/TIMP-1 in the two groups were significantly lower than those before treatment, and the levels of TIMP-1, MMP-9 and MMP-9/TIMP-1 in the observation group were significantly lower than those in the control group;the levels of SF and LPO in the two groups were significantly lower than those before treatment, while the levels of SOD in the observation group were significantly higher than those before treatment, and the levels of SF and LPO in the observation group were significantly lower than those in the control group, while the levels of SOD in the observation group were significantly higher than those in the control group. Conclusions:Intensive insulin therapy can effectively improve the immune function of severe pneumonia patients, reduce their inflammatory reaction, matrix metalloproteinase levels and oxidative stress injury.

Effects of adjuvant raw rhubarb enema therapy on systemic inflammatory stress response and intestinal mucosal barrier function in patients with severe pancreatitis

Objective: To investigate the effects of adjuvant raw rhubarb enema therapy on systemic inflammatory stress response and intestinal mucosal barrier function in patients with severe pancreatitis. Methods: A total of 78 patients with severe pancreatitis treated in the Second People's Hospital of Neijiang, Sichuan, China between December 2016 and September 2018 were included in the study. The patients were divided into control group (n=39) and raw rhubarb group (n=39) by simple randomization. The control group of patients received conventional treatment of severe pancreatitis with western medicine, while the raw rhubarb group of patients received raw rhubarb enema on the basis of the treatment of control group, and the efficacy was evaluated after continuous treatment for 1 week. The differences in serum levels of inflammatory mediators (high mobility group protein B1, C-reactive protein, prostaglandin E2 and tumor necrosis factor α), stress hormones (cortisol and epinephrine) as well as intestinal mucosal barrier function indicators (D-lactate, endotoxin and diamine oxidase) were compared between the two groups of patients before and after treatment. Results: Before treatment, there was no statistically significant difference in serum levels of inflammatory mediators, stress hormones and intestinal mucosal barrier function indicators between the two groups (P>0.05). After treatment, serum high mobility group protein B1, C-reactive protein, prostaglandin E2 and tumor necrosis factor levels of the observation group were significantly lower than those of the control group;cortisol and epinephrine levels were significantly lower than those of the control group;D-lactate, endotoxin and diamine oxidase levels were significantly lower than those of the control group (P<0.05). Conclusions:Adjuvant raw rhubarb enema therapy on the basis of western medicine can help alleviate the inflammatory stress response and optimize the intestinal mucosal barrier function in patients with severe pancreatitis.

Effect of ulinastatin + thymosin adjuvant therapy on inflammatory and stress response in patients with severe pneumonia

Objective: To investigate the effect of ulinastatin + thymosin adjuvant therapy on inflammatory and stress response in patients with severe pneumonia. Methods: A total of 66 patients with severe pneumonia who were diagnosed and treated in Huanggang Central Hospital between July 2016 and July 2017 were divided into control group (n=33) and study group (n=33) by random number table. Control group received routine therapy for severe pneumonia, and study group received routine therapy combined with ulinastatin + thymosin adjuvant therapy, which lasted for 1 week. The differences in serum levels of inflammatory factors and stress hormones were compared between the two groups before and after treatment. Results: Before treatment, the differences in serum levels of inflammatory factors and stress hormones were not statistically significant between the two groups. After 1 week of treatment, serum levels of inflammatory factors and stress hormones of both groups of patients were lower than those before treatment, serum levels of pro-inflammatory factors IL-1β, IL-2, IL-6, IL-8 and TNF-α as well as anti-inflammatory factors IL-4, IL-10 and IL-13 of study group were lower than those of control group, and serum stress hormones AngⅠ, AngⅡ, NE and Cor levels were lower than those of control group. Conclusion: Routine therapy combined with ulinastatin + thymosin adjuvant therapy can further inhibit the systemic inflammatory response and stress response and optimize the overall condition in patients with severe pneumonia.