The mechanisms underlying sex determination and differentiation have long intrigued researchers in the fields of development and evolutionary biology.The roughskin sculpin(Trachidermus fasciatus Heckel),displaying sex...The mechanisms underlying sex determination and differentiation have long intrigued researchers in the fields of development and evolutionary biology.The roughskin sculpin(Trachidermus fasciatus Heckel),displaying sexual dimorphism,provides an ideal model for studying the mechanisms.However,both genetic and genomic information concerning sex determination and differentiation,such as gonadal transcriptome data in roughskin sculpin,are lacking.Here,we present the first gonadal transcriptomes of roughskin sculpin and identify sex-related genes.We identified 8531 differentially expressed genes(DEGs),among them 4065 were upregulated in the ovary and 4466 upregulated in the testis.Several sex-related gene ontology(GO)terms were enriched in ovary-biased genes,including“binding of sperm to zona pellucida”,“egg coat formation”,“positive regulation of acrosome reaction”,“cell division”,and“cell cycle”,while the GO terms such as“spermatogenesis”,“sperm axoneme assembly”,“cilium assembly”,“cilium movement”,and“cilium movement involved in cell motility”were enriched in testis-biased genes.Moreover,six KEGG pathways were significantly enriched in the ovary,whereas only one was enriched in the testis.Of these DEGs,40 sex-related genes were identified,which including 26 testis-biased genes(such as Dmrtb 1,Gsdf,Sox 9 b,Wnt 4 b,Tcp 11 l 2,and Efhb),and 14 ovary-biased genes(such as Cyp 19 a 1 a,Foxh 1,Foxr 1,Gdf 3,Hsd 17 b 12,and Igf 2 bp 3).This gonadal transcript dataset would broaden our understanding of sex determination and differentiation mechanisms in roughskin sculpin,expand the genomic database,support future studies on sex-related gene functions,and facilitate molecular biology research into roughskin sculpin.展开更多
为探讨角蛋白关联蛋白6.2(keratin-associated protein 6.2,KAP6.2)基因遗传多态性与内蒙古绒山羊生长性状和绒细度性状的相关性,以期为内蒙古绒山羊遗传育种改良提供理论依据,本研究采集598只雌性内蒙古绒山羊耳组织,提取基因组DNA并进...为探讨角蛋白关联蛋白6.2(keratin-associated protein 6.2,KAP6.2)基因遗传多态性与内蒙古绒山羊生长性状和绒细度性状的相关性,以期为内蒙古绒山羊遗传育种改良提供理论依据,本研究采集598只雌性内蒙古绒山羊耳组织,提取基因组DNA并进行PCR扩增,检测KAP6.2基因的插入/缺失(Insertion/Deletion,InDel)突变,分析突变位点与生长性状和绒细度性状的相关性。结果显示,在育成羊中,该突变位点与胸围和十字部显著相关(P<0.05),与体长、胸宽和胸深极显著相关(P<0.01);在成年羊中,该突变位点与胸宽显著相关(P<0.05),与胸围、十字部高和胸深极显著相关(P<0.01);在育成羊和成年羊全部群体中,该突变位点与体长、胸围、十字部高、胸深和胸宽极显著相关(P<0.01),与绒细度显著相关(P<0.05)。上述结果提示,KAP6.2基因外显子区24 bp InDel突变位点与内蒙古绒山羊部分生长性状显著或极显著相关,与绒细度显著相关,可作为内蒙古绒山羊部分生长性状和绒细度性状选育的遗传标记,为内蒙古绒山羊的选育提供科学依据。展开更多
KRAB相关蛋白1(KRAB-associated protein 1,KAP1)是最早于1996年通过亲合色谱分离并克隆得到的一种转录辅因子,因能与含KRAB结构域的锌指蛋白家族(zinc family proteins,ZFPs)成员结合而得名。KAP1是一种具有多功能的蛋白质,它参与组蛋...KRAB相关蛋白1(KRAB-associated protein 1,KAP1)是最早于1996年通过亲合色谱分离并克隆得到的一种转录辅因子,因能与含KRAB结构域的锌指蛋白家族(zinc family proteins,ZFPs)成员结合而得名。KAP1是一种具有多功能的蛋白质,它参与组蛋白修饰与染色质重塑、调节DNA甲基化、作为转录共调控因子参与基因调控、参与DNA损伤反应等,并存在磷酸化、乙酰化等多种翻译后修饰。KAP1的表达水平与多种人类恶性肿瘤的发生发展密切相关,并影响肿瘤预后。因此,KAP1有望成为恶性肿瘤的诊断标志物或治疗新靶点。本文主要综述KAP1的结构、功能及其与人类恶性肿瘤发生发展的关系,并进一步探讨KAP1在恶性肿瘤研究中的应用前景,以期为恶性肿瘤的临床诊断和治疗提供参考依据。展开更多
目的对飞行人员营养知识、态度、行为(knowledge,attitude and practice,KAP)状况和膳食营养情况进行调查,并结合体检结果进行分析,以了解飞行人员的营养知信行、膳食营养和身体健康等现状,为做好飞行人员的膳食营养保障工作提供依据和...目的对飞行人员营养知识、态度、行为(knowledge,attitude and practice,KAP)状况和膳食营养情况进行调查,并结合体检结果进行分析,以了解飞行人员的营养知信行、膳食营养和身体健康等现状,为做好飞行人员的膳食营养保障工作提供依据和方向。方法采用随机抽样方法,选取2021年3月一2022年3月空军杭州特勤疗养中心和海军青岛特勤疗养中心438名飞行人员作为调查对象,进行营养知信行问卷调查,并对其膳食采用称重法,连续3d进行膳食营养状况调查,同时进行健康体检。结果飞行人员KAP最高得分46分,最低得分30分,平均得分(39.26±4.03)分。飞行人员各类食物摄入量与标准推荐量比较,除畜肉、禽肉和植物油摄入超标外,谷类、水产类、水果、豆类和奶类制品摄入量均未达标。能量和营养素摄入情况显示,飞行人员能量、蛋白质、钠、钾、镁、磷、铁、硒、维生素E、维生素A和烟酸摄入超标,维生素B_(2)、维生素B_(1)和锌摄入不足。三大产能营养素供能比与标准推荐摄入量比较,飞行人员蛋白质和脂肪供能比偏高,碳水化合物的供能比偏低。体检异常者348名,占79.45%,其中超重、高尿酸血症、脂肪肝和高脂血症的检出率分别为39.04%、16.21%、9.13%和7.08%。结论飞行人员营养KAP水平偏低,膳食结构不均衡,超重、高尿酸血症、脂肪肝和高脂血症等营养相关疾病患病率较高,且三者之间具有较高相关性。应对飞行人员积极开展饮食营养健康教育,加强科学配餐指导,降低与营养相关慢性病的发病率。展开更多
Objective:To explore the impact of a continuous precision nursing model on patients’Knowledge,Attitudes,and Practices(KAP)and cardiac function during the nursing process of patients undergoing percutaneous coronary a...Objective:To explore the impact of a continuous precision nursing model on patients’Knowledge,Attitudes,and Practices(KAP)and cardiac function during the nursing process of patients undergoing percutaneous coronary angiography and stent implantation.Methods:Ninety patients who underwent percutaneous coronary angiography and stent implantation in our hospital from April 2022 to April 2023 were selected and randomly divided into the control group(45 cases),in which routine nursing support was carried out during the treatment process,and the observation group(45 cases),in which continuous precision nursing model was carried out during the treatment process.Comparisons were made between the two groups of patients on their KAP,cardiac function,and quality of life during recovery.Results:There was no difference in the left ventricular ejection fraction(LVEF),cardiac output(CO),and cardiac index(CI)levels before intervention.After the intervention,the levels of cardiac function in the observation group were higher than those of the control group(P<0.05).There was no difference in the Exercise of Self-Care Agency(ESCA)self-care ability scale scores before the intervention.After the intervention,the observation group had higher ESCA scores than the control group(P<0.05).Conclusion:Implementation of a continuous precision nursing model in the care of patients undergoing percutaneous coronary angiography and stent implantation improved the patient’s cardiac function,and KAP,and promoted recovery.展开更多
Objective To explore the influence of parental factors on sexual and reproductive health awareness,attitudes to pre-marital sex, and pre-marital sexual activity among unmarried youthMethods Data were collected by anon...Objective To explore the influence of parental factors on sexual and reproductive health awareness,attitudes to pre-marital sex, and pre-marital sexual activity among unmarried youthMethods Data were collected by anonymous self-completed questionnaire survey among eli-gible unmarried youth aged 15~22 in Chengdu City, Southwest China during 1998~1999 andthe study comprised of 3 307 valid subjects.Results Awareness of safe sex and contraception among unmarried youth was limited and theirattitudes towards pre-marital sexual activity appeared to be positive. Large proportions of dat-ing subjects were sexually active and reported ever-using contraceptives, regular or appropriateuse of contraceptives was likely to be limited. About two thirds of adolescents had communica-tion with their parents frequently, but fewer had communicated with their parents on sex-relatedissues. but they did influence the sexual behaviours of adolescents. In fact, it appeared that co-residence and extensive communication with parents on sex-related issues could delay sexualactivity among adolescents, and the sexually active youth co-resided with their parents weremore likely to take contraceptive measures.Conclusion Programmes are needed not only for youth to encourage them to communicate withtheir parents on sex-related issues, but also for parents to know the current sexual health statusof youth and realize the necessity to communicate with their adolescent children about sexualand reproductive health issues.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.31972793,31502169)the Key Scientific Research Project in Universities and Colleges in Tianjin(No.2022ZD004)。
文摘The mechanisms underlying sex determination and differentiation have long intrigued researchers in the fields of development and evolutionary biology.The roughskin sculpin(Trachidermus fasciatus Heckel),displaying sexual dimorphism,provides an ideal model for studying the mechanisms.However,both genetic and genomic information concerning sex determination and differentiation,such as gonadal transcriptome data in roughskin sculpin,are lacking.Here,we present the first gonadal transcriptomes of roughskin sculpin and identify sex-related genes.We identified 8531 differentially expressed genes(DEGs),among them 4065 were upregulated in the ovary and 4466 upregulated in the testis.Several sex-related gene ontology(GO)terms were enriched in ovary-biased genes,including“binding of sperm to zona pellucida”,“egg coat formation”,“positive regulation of acrosome reaction”,“cell division”,and“cell cycle”,while the GO terms such as“spermatogenesis”,“sperm axoneme assembly”,“cilium assembly”,“cilium movement”,and“cilium movement involved in cell motility”were enriched in testis-biased genes.Moreover,six KEGG pathways were significantly enriched in the ovary,whereas only one was enriched in the testis.Of these DEGs,40 sex-related genes were identified,which including 26 testis-biased genes(such as Dmrtb 1,Gsdf,Sox 9 b,Wnt 4 b,Tcp 11 l 2,and Efhb),and 14 ovary-biased genes(such as Cyp 19 a 1 a,Foxh 1,Foxr 1,Gdf 3,Hsd 17 b 12,and Igf 2 bp 3).This gonadal transcript dataset would broaden our understanding of sex determination and differentiation mechanisms in roughskin sculpin,expand the genomic database,support future studies on sex-related gene functions,and facilitate molecular biology research into roughskin sculpin.
文摘为探讨角蛋白关联蛋白6.2(keratin-associated protein 6.2,KAP6.2)基因遗传多态性与内蒙古绒山羊生长性状和绒细度性状的相关性,以期为内蒙古绒山羊遗传育种改良提供理论依据,本研究采集598只雌性内蒙古绒山羊耳组织,提取基因组DNA并进行PCR扩增,检测KAP6.2基因的插入/缺失(Insertion/Deletion,InDel)突变,分析突变位点与生长性状和绒细度性状的相关性。结果显示,在育成羊中,该突变位点与胸围和十字部显著相关(P<0.05),与体长、胸宽和胸深极显著相关(P<0.01);在成年羊中,该突变位点与胸宽显著相关(P<0.05),与胸围、十字部高和胸深极显著相关(P<0.01);在育成羊和成年羊全部群体中,该突变位点与体长、胸围、十字部高、胸深和胸宽极显著相关(P<0.01),与绒细度显著相关(P<0.05)。上述结果提示,KAP6.2基因外显子区24 bp InDel突变位点与内蒙古绒山羊部分生长性状显著或极显著相关,与绒细度显著相关,可作为内蒙古绒山羊部分生长性状和绒细度性状选育的遗传标记,为内蒙古绒山羊的选育提供科学依据。
文摘KRAB相关蛋白1(KRAB-associated protein 1,KAP1)是最早于1996年通过亲合色谱分离并克隆得到的一种转录辅因子,因能与含KRAB结构域的锌指蛋白家族(zinc family proteins,ZFPs)成员结合而得名。KAP1是一种具有多功能的蛋白质,它参与组蛋白修饰与染色质重塑、调节DNA甲基化、作为转录共调控因子参与基因调控、参与DNA损伤反应等,并存在磷酸化、乙酰化等多种翻译后修饰。KAP1的表达水平与多种人类恶性肿瘤的发生发展密切相关,并影响肿瘤预后。因此,KAP1有望成为恶性肿瘤的诊断标志物或治疗新靶点。本文主要综述KAP1的结构、功能及其与人类恶性肿瘤发生发展的关系,并进一步探讨KAP1在恶性肿瘤研究中的应用前景,以期为恶性肿瘤的临床诊断和治疗提供参考依据。
文摘目的对飞行人员营养知识、态度、行为(knowledge,attitude and practice,KAP)状况和膳食营养情况进行调查,并结合体检结果进行分析,以了解飞行人员的营养知信行、膳食营养和身体健康等现状,为做好飞行人员的膳食营养保障工作提供依据和方向。方法采用随机抽样方法,选取2021年3月一2022年3月空军杭州特勤疗养中心和海军青岛特勤疗养中心438名飞行人员作为调查对象,进行营养知信行问卷调查,并对其膳食采用称重法,连续3d进行膳食营养状况调查,同时进行健康体检。结果飞行人员KAP最高得分46分,最低得分30分,平均得分(39.26±4.03)分。飞行人员各类食物摄入量与标准推荐量比较,除畜肉、禽肉和植物油摄入超标外,谷类、水产类、水果、豆类和奶类制品摄入量均未达标。能量和营养素摄入情况显示,飞行人员能量、蛋白质、钠、钾、镁、磷、铁、硒、维生素E、维生素A和烟酸摄入超标,维生素B_(2)、维生素B_(1)和锌摄入不足。三大产能营养素供能比与标准推荐摄入量比较,飞行人员蛋白质和脂肪供能比偏高,碳水化合物的供能比偏低。体检异常者348名,占79.45%,其中超重、高尿酸血症、脂肪肝和高脂血症的检出率分别为39.04%、16.21%、9.13%和7.08%。结论飞行人员营养KAP水平偏低,膳食结构不均衡,超重、高尿酸血症、脂肪肝和高脂血症等营养相关疾病患病率较高,且三者之间具有较高相关性。应对飞行人员积极开展饮食营养健康教育,加强科学配餐指导,降低与营养相关慢性病的发病率。
文摘Objective:To explore the impact of a continuous precision nursing model on patients’Knowledge,Attitudes,and Practices(KAP)and cardiac function during the nursing process of patients undergoing percutaneous coronary angiography and stent implantation.Methods:Ninety patients who underwent percutaneous coronary angiography and stent implantation in our hospital from April 2022 to April 2023 were selected and randomly divided into the control group(45 cases),in which routine nursing support was carried out during the treatment process,and the observation group(45 cases),in which continuous precision nursing model was carried out during the treatment process.Comparisons were made between the two groups of patients on their KAP,cardiac function,and quality of life during recovery.Results:There was no difference in the left ventricular ejection fraction(LVEF),cardiac output(CO),and cardiac index(CI)levels before intervention.After the intervention,the levels of cardiac function in the observation group were higher than those of the control group(P<0.05).There was no difference in the Exercise of Self-Care Agency(ESCA)self-care ability scale scores before the intervention.After the intervention,the observation group had higher ESCA scores than the control group(P<0.05).Conclusion:Implementation of a continuous precision nursing model in the care of patients undergoing percutaneous coronary angiography and stent implantation improved the patient’s cardiac function,and KAP,and promoted recovery.
文摘Objective To explore the influence of parental factors on sexual and reproductive health awareness,attitudes to pre-marital sex, and pre-marital sexual activity among unmarried youthMethods Data were collected by anonymous self-completed questionnaire survey among eli-gible unmarried youth aged 15~22 in Chengdu City, Southwest China during 1998~1999 andthe study comprised of 3 307 valid subjects.Results Awareness of safe sex and contraception among unmarried youth was limited and theirattitudes towards pre-marital sexual activity appeared to be positive. Large proportions of dat-ing subjects were sexually active and reported ever-using contraceptives, regular or appropriateuse of contraceptives was likely to be limited. About two thirds of adolescents had communica-tion with their parents frequently, but fewer had communicated with their parents on sex-relatedissues. but they did influence the sexual behaviours of adolescents. In fact, it appeared that co-residence and extensive communication with parents on sex-related issues could delay sexualactivity among adolescents, and the sexually active youth co-resided with their parents weremore likely to take contraceptive measures.Conclusion Programmes are needed not only for youth to encourage them to communicate withtheir parents on sex-related issues, but also for parents to know the current sexual health statusof youth and realize the necessity to communicate with their adolescent children about sexualand reproductive health issues.
