[ Objective] The aim was to establish the method of extracting genomic DNA from sheep blood clot on the basis of the improvement of method for extracting genomic DNA from tissues. [Method]The genomic DNA with complete...[ Objective] The aim was to establish the method of extracting genomic DNA from sheep blood clot on the basis of the improvement of method for extracting genomic DNA from tissues. [Method]The genomic DNA with complete primary structure and high purity was obtained from the sheep blood clot after the steps of cutting the sheep blood clot with ophthalmic scissors, cell lysis with tissue DNA extracts and digested by proteinase K, extracting with phenol/chloroform and precipitating with ethanol were performed. [ Result] The concentration of the extracted DNA was 159.90 ±0.70 ng/μl and the ratio of the A260/A280 was 1.80 ＋0.01. The sheep microsatellite locus of BM203 was amplified by using the extracted DNA from the sheep blood clot as template of PCR, and the PCR result was perfect. [Conclusion]This method is simple and feasible, the quantity and quality of the extracted DNA can satisfy the demands for the subsequent researches. It is worth to extending and using for reference.展开更多
The objective of this study was to investigate the effect of long-term grazing sheep in cotton stubble on variation of biochemistry components and free gossypol residual in the blood. The survey found that 15% ewe of ...The objective of this study was to investigate the effect of long-term grazing sheep in cotton stubble on variation of biochemistry components and free gossypol residual in the blood. The survey found that 15% ewe of grazing group suffered miscarriage or premature birth,compared with the captive group ewes without this phenomenon. Blood test result shows that the free gossypol concentration in serum of grazing group was significantly higher than in indoor captive feeding group( P < 0. 01). In order to ensure the health of sheep grazing on cotton stubble and improve breeding rate,there is a need to do further reasonable research of grazing and feeding methods.展开更多
This investigation included two experiments. Experiment 1 was executed to study the effect of feeding different rations of protected protein of canola meal on digestibility and nutritive values within sheep. Twenty ma...This investigation included two experiments. Experiment 1 was executed to study the effect of feeding different rations of protected protein of canola meal on digestibility and nutritive values within sheep. Twenty male, healthy sheep were divided into five treatments according to the methods of protein protection (control, heat, sodium hydroxide, formaldehyde, and acetic acid treatments). Experiment 2 was carried out on developing lambs to investigate the effect of protected protein on growth performance and some blood metabolites. Animals in this ex-periment were also divided into the same treatments as Experiment 1. Animals in the first and second experiment were fed concentrate ration (80%) and wheat straw (20%) to cover the feed requirements. Nutritive values expressed as total digestible nutrients (TDN %) and digestible crude protein (DCP%) of the experimental rations was calculated. In the second experimental all animals were weighed biweekly and the amounts of rations were adjusted throughout the experimental period (120 days) according to their body weight change. Results indicated that in the first experimental protected protein by heat (HE) and sodium hydroxide (NH) had positive (P<0.05) effects on most of digestibility coefficients of different nutrients. Protein protection methods also improved (P<0.05) the nutritive values (TDN and DCP) in the HE treatment and NH treatment. In the second experiment body weight increased by 14% and 7% and also daily gain by 27% and14 % in HE and NH, respectively, while FM and AC decreased body weight by 8% and 4.4%. Higher values (P<0.01) in both thyroid hormones were observed in HE and NH than those other treatments. Also, higher values (P<0.01) of total protein, albumin, and glucose were observed in HE and NH than other treatments. The control (CTL) group recorded higher concentrations of urea-N and creatinine at different periods of the experiment in comparison with other treatments. Generally, from the present investigation it can be concluded that protected protein of canola meal by heat or sodium hydroxide treatments were more efficient for productive performance and some blood metabolites of sheep.展开更多
[ Objective] The research was aimed to provide the basis for the development and utilization of Guide black-fur sheep and its disease di- agnosis and prevention. [ Methed] Blood samples were collected from 15 healthy ...[ Objective] The research was aimed to provide the basis for the development and utilization of Guide black-fur sheep and its disease di- agnosis and prevention. [ Methed] Blood samples were collected from 15 healthy adult Guide black-fur sheep in Black-Fur Sheep Farm o＇f Guinan County in Qinghai Province. Eighteen blood indices belonging to three classes, erythrocyte, leukocyte and platelet, were determined. E Resultl The blood indices of the Guide black-fur sheep were as follows： the white blood cell count, （7.52 ±2.72） × 109/L; the red blood cell count, （13.04 ± 2.81 ） × 1012/L; the mean corpuscular hemoglobin, （85.36 ＋27.05） g/L; the mean corpuscular volume, （37.22 ± 1.93） fL; the mean corpuscular hemoglobin concentration, （13.19 ±0.96） pg; the platelet count, （370.67 ± 139.60） × 109/L; the lymphocyte absolute count, （14.11 ＋9.36） x 109/L; the absolute count of intermediate cell, （0.79 ±0.46）× 109/L; and the absolute neutrophil count, （0.81 ＋0.71 ） ×109/L.- Conclusion] The reseamh lays the foundation for further Drotectina the aenetic resources of the Guide black-fur sheep and imDrovinQ their production performance.展开更多
An experiment arranged in 2×3 (2 concentrate supplement levels (CSL) and 3 breeds) factorial was carried out to evaluate the effect of CSL, breeds, and their interaction on feed intake, digestibility, feed utiliz...An experiment arranged in 2×3 (2 concentrate supplement levels (CSL) and 3 breeds) factorial was carried out to evaluate the effect of CSL, breeds, and their interaction on feed intake, digestibility, feed utilization efficiency, and blood profiles of Ethiopian fat-tail hair sheep. The CSL were 1% and 1.75% body weight, designated as L1 and L2, respectively. The breeds used were yearling intact male Blackhead Ogaden (BHO), Horro and Washera. Sixteen sheep from each breed were divided into two based on initial body weight and randomly allocated to two dietary treatments for digestibility and growth trials of 10 and 90 days, respectively. Grass hay as a basal diet was offered ad libitum to each sheep. Feed intake every day and live body weight at interval of ten days were recorded for the duration of the experiment. Blood samples and linear body measurements were taken a week before the end and on the last day of the experiment from all sheep, respectively. Dry matter and nutrient intakes, except concentrate supplement and CP, were influenced by the interaction. The higher (p<0.0001) DM digestibility was recorded from sheep supplemented with L2 than L1. Consequently, sheep supplemented with L2 had significantly higher (P<0.05) feed conversion efficiency (FCE), average daily gain (ADG) (57.3 vs 44.2 kg), total gain (TG) (5.3 vs 3.9 kg) and final body weight (FBW) than those supplemented with L1. Among breeds, Horro sheep recorded higher (P<0.05) DM intake, digestibility, and FCE than the rest of the breeds. Consequently, this breed has significantly (p<0.05) higher TG, ADG, and FBW. Some of the blood hematology (PCV, MCV, neutrophils, and monocytes) and sera metabolites (total protein, urea, and cholesterol) values were influenced by the interaction in favor of L2. Hemoglobin concentration was higher for Washera than the other breeds (p0.05). In conclusion, the study indicate the presence of variation in nutrient intake and utilization, performance traits, and blood profiles among breeds and between CSL, most of which were in favor of Horro breed and high level of concentrate.展开更多
基金Supported by Natural Science Foundation of Shanxi Province(2007011081 )Returning Brains Project in Shanxi Province(2007066 )Agricultural Science and Technology Achievement Transformation Fund Project(2008GB2A300032)~~
文摘[ Objective] The aim was to establish the method of extracting genomic DNA from sheep blood clot on the basis of the improvement of method for extracting genomic DNA from tissues. [Method]The genomic DNA with complete primary structure and high purity was obtained from the sheep blood clot after the steps of cutting the sheep blood clot with ophthalmic scissors, cell lysis with tissue DNA extracts and digested by proteinase K, extracting with phenol/chloroform and precipitating with ethanol were performed. [ Result] The concentration of the extracted DNA was 159.90 ±0.70 ng/μl and the ratio of the A260/A280 was 1.80 ＋0.01. The sheep microsatellite locus of BM203 was amplified by using the extracted DNA from the sheep blood clot as template of PCR, and the PCR result was perfect. [Conclusion]This method is simple and feasible, the quantity and quality of the extracted DNA can satisfy the demands for the subsequent researches. It is worth to extending and using for reference.
基金Supported by National Natural Science Foundation of China(31360565)
文摘The objective of this study was to investigate the effect of long-term grazing sheep in cotton stubble on variation of biochemistry components and free gossypol residual in the blood. The survey found that 15% ewe of grazing group suffered miscarriage or premature birth,compared with the captive group ewes without this phenomenon. Blood test result shows that the free gossypol concentration in serum of grazing group was significantly higher than in indoor captive feeding group( P < 0. 01). In order to ensure the health of sheep grazing on cotton stubble and improve breeding rate,there is a need to do further reasonable research of grazing and feeding methods.
文摘This investigation included two experiments. Experiment 1 was executed to study the effect of feeding different rations of protected protein of canola meal on digestibility and nutritive values within sheep. Twenty male, healthy sheep were divided into five treatments according to the methods of protein protection (control, heat, sodium hydroxide, formaldehyde, and acetic acid treatments). Experiment 2 was carried out on developing lambs to investigate the effect of protected protein on growth performance and some blood metabolites. Animals in this ex-periment were also divided into the same treatments as Experiment 1. Animals in the first and second experiment were fed concentrate ration (80%) and wheat straw (20%) to cover the feed requirements. Nutritive values expressed as total digestible nutrients (TDN %) and digestible crude protein (DCP%) of the experimental rations was calculated. In the second experimental all animals were weighed biweekly and the amounts of rations were adjusted throughout the experimental period (120 days) according to their body weight change. Results indicated that in the first experimental protected protein by heat (HE) and sodium hydroxide (NH) had positive (P<0.05) effects on most of digestibility coefficients of different nutrients. Protein protection methods also improved (P<0.05) the nutritive values (TDN and DCP) in the HE treatment and NH treatment. In the second experiment body weight increased by 14% and 7% and also daily gain by 27% and14 % in HE and NH, respectively, while FM and AC decreased body weight by 8% and 4.4%. Higher values (P<0.01) in both thyroid hormones were observed in HE and NH than those other treatments. Also, higher values (P<0.01) of total protein, albumin, and glucose were observed in HE and NH than other treatments. The control (CTL) group recorded higher concentrations of urea-N and creatinine at different periods of the experiment in comparison with other treatments. Generally, from the present investigation it can be concluded that protected protein of canola meal by heat or sodium hydroxide treatments were more efficient for productive performance and some blood metabolites of sheep.
基金funded by Research Fund for Young and Middle-Aged Talent of Northwest University for Nationalities(XBMU-2006-BD-81)
文摘[ Objective] The research was aimed to provide the basis for the development and utilization of Guide black-fur sheep and its disease di- agnosis and prevention. [ Methed] Blood samples were collected from 15 healthy adult Guide black-fur sheep in Black-Fur Sheep Farm o＇f Guinan County in Qinghai Province. Eighteen blood indices belonging to three classes, erythrocyte, leukocyte and platelet, were determined. E Resultl The blood indices of the Guide black-fur sheep were as follows： the white blood cell count, （7.52 ±2.72） × 109/L; the red blood cell count, （13.04 ± 2.81 ） × 1012/L; the mean corpuscular hemoglobin, （85.36 ＋27.05） g/L; the mean corpuscular volume, （37.22 ± 1.93） fL; the mean corpuscular hemoglobin concentration, （13.19 ±0.96） pg; the platelet count, （370.67 ± 139.60） × 109/L; the lymphocyte absolute count, （14.11 ＋9.36） x 109/L; the absolute count of intermediate cell, （0.79 ±0.46）× 109/L; and the absolute neutrophil count, （0.81 ＋0.71 ） ×109/L.- Conclusion] The reseamh lays the foundation for further Drotectina the aenetic resources of the Guide black-fur sheep and imDrovinQ their production performance.
文摘An experiment arranged in 2×3 (2 concentrate supplement levels (CSL) and 3 breeds) factorial was carried out to evaluate the effect of CSL, breeds, and their interaction on feed intake, digestibility, feed utilization efficiency, and blood profiles of Ethiopian fat-tail hair sheep. The CSL were 1% and 1.75% body weight, designated as L1 and L2, respectively. The breeds used were yearling intact male Blackhead Ogaden (BHO), Horro and Washera. Sixteen sheep from each breed were divided into two based on initial body weight and randomly allocated to two dietary treatments for digestibility and growth trials of 10 and 90 days, respectively. Grass hay as a basal diet was offered ad libitum to each sheep. Feed intake every day and live body weight at interval of ten days were recorded for the duration of the experiment. Blood samples and linear body measurements were taken a week before the end and on the last day of the experiment from all sheep, respectively. Dry matter and nutrient intakes, except concentrate supplement and CP, were influenced by the interaction. The higher (p<0.0001) DM digestibility was recorded from sheep supplemented with L2 than L1. Consequently, sheep supplemented with L2 had significantly higher (P<0.05) feed conversion efficiency (FCE), average daily gain (ADG) (57.3 vs 44.2 kg), total gain (TG) (5.3 vs 3.9 kg) and final body weight (FBW) than those supplemented with L1. Among breeds, Horro sheep recorded higher (P<0.05) DM intake, digestibility, and FCE than the rest of the breeds. Consequently, this breed has significantly (p<0.05) higher TG, ADG, and FBW. Some of the blood hematology (PCV, MCV, neutrophils, and monocytes) and sera metabolites (total protein, urea, and cholesterol) values were influenced by the interaction in favor of L2. Hemoglobin concentration was higher for Washera than the other breeds (p0.05). In conclusion, the study indicate the presence of variation in nutrient intake and utilization, performance traits, and blood profiles among breeds and between CSL, most of which were in favor of Horro breed and high level of concentrate.
