The present experiment was conducted to determine the effects of Molasses-Urea Supplementation （MUS） on weight gain, ruminal fermentation and major microbial populations in sheep on a winter grazing regime in Inner ...The present experiment was conducted to determine the effects of Molasses-Urea Supplementation （MUS） on weight gain, ruminal fermentation and major microbial populations in sheep on a winter grazing regime in Inner Mongolia. Total 40 sheep, allowed free consumption of MUS after grazing, served as a treatment group, while 30 sheep, fed only by pasture grazing, served as a control group. Ruminal fermentation parameters, consisted of pH, Bacterial Crude Protein （BCP） and ammonia nitrogen （NH3-N） were measured. In addition, numbers of five symbiotic bacteria were investigated. The results showed as follows： the average daily weight gain, concentration of NH3-N and numbers of protozoa were significantly higher （p〈0.05） in the treatment group than those in the control group. Contrastingly, no significant difference was found in BCP concentration and pH between the two groups. At the end of the experiment, the populations of Selenomonas ruminantium, Anaerovibrio lipolytica, Fibrobacter succinogenes, Ruminococcus flaveciens and Ruminococcus albus in the treatment group were significantly higher than those of the control group （p〈0.05）. These results demonstrated that greater weight gain could be induced during winter in Inner Mongolia by improved nutritional status through promotion of microbial populations using urea and sugar.展开更多
为了解脂尾绵羊的尾部性状特征,该试验选择滩羊典型生产区开展调查和试验观测,通过测量滩羊尾部尺寸、重量及尾骨特征,分析滩羊尾型特征及脂尾性状间的表型相关。结果显示:滩羊尾型可分为圆盘形尾、楔形尾和三角形尾3种;滩羊尾椎数量为1...为了解脂尾绵羊的尾部性状特征,该试验选择滩羊典型生产区开展调查和试验观测,通过测量滩羊尾部尺寸、重量及尾骨特征,分析滩羊尾型特征及脂尾性状间的表型相关。结果显示:滩羊尾型可分为圆盘形尾、楔形尾和三角形尾3种;滩羊尾椎数量为17~21枚不等:根据尾椎椎骨的完整程度,滩羊的尾椎可分为4类,第1类尾椎的横突、棘突、椎弓、椎孔完整,第2类尾椎的横突完整,棘突、椎弓、椎孔退化,第3类尾椎的横突、棘突、椎弓、椎孔均发生退化,第4类尾椎椎骨之间融合,无关节囊。滩羊尾重与尾厚,尾脂率与尾重及尾脂重之间相关性显著。滩羊尾重与尾长、尾厚之间的最优回归方程为:y=-2.237+0.401 x 3+0.041 x 1(R 2=0.634,P<0.01)。展开更多
[ Objective] The study aimed to analyze the expression and polymorphism of KAP6.1 gene in various tissues of sheep, as well as the correlation between KAP6.1 gene and wool traits, so as to provide scientific reference...[ Objective] The study aimed to analyze the expression and polymorphism of KAP6.1 gene in various tissues of sheep, as well as the correlation between KAP6.1 gene and wool traits, so as to provide scientific references for the further study on the functions of KAP6.1 gene and molecular breeding of fine wool sheep. [Method] By using real-time fluorescence quantitative PCR, the expression map of KAP6.1 gene in various tissues was analyzed, and then PCR-SSCP technology, cloning and sequencing were adopted to analyze the polymorphism of KAP6.1 gene in 693 Chinese merino sheep （Xinjiang Junken type）, finally the correlation between KAPS. 1 gene and wool traits was discussed. [ Result] A high -level expression of KAP6.1 gene appeared in sheep skin, very significantly higher than that in muscle, small intestines, ovaries, hearts, lungs, livers, spleens, kidneys and rumen （ P 〈0.01 ）. In addition, there was C159T base substitution in KAF6.1 gene sequence, and analysis of the least squares model showed that the mean wool fleece weight of BB genotype at C159T site was significantly higher than that of AA and AB genotype （ P 〈0.05）, while there was no significant difference in average wool fiber diameter, curvature, length, clean fleece rate and density among hA, BB and AB genotype of KAP6.1 gene at C159T site （P〉0.05）. [Conclusion] KAP6.1 gene could be as the candidate gene of wool yield of sheep, and BB genotype could be used as the important molecular marker of fine wool sheep for high wool yield.展开更多
Sheep meat production in the dry tropics of Mexico is low in the dry season, <span>due to lack of forage, therefore, strategic supplementation is important to av</span>oid weight loss in animals;The resear...Sheep meat production in the dry tropics of Mexico is low in the dry season, <span>due to lack of forage, therefore, strategic supplementation is important to av</span>oid weight loss in animals;The research was carried out from August 15 to De<span>cember 15, 2020 in order to evaluate the productive response of pelibuey sh</span>eep in confinement, fed with pangola grass hay (GH), plains grass silage (PGS) and supplemented with commercial feed (CF) in the dry season. 16 sheep of the same weight and age were used, four treatments were evaluated in them, in a completely random design;the treatments were four levels of supplement 0, 0.1, 0.2 and 0.3 kg of AC fed with HP and PGS. Sheep were dewormed at the beginning of the experiment and housed in individual cages. The variables were: dry matter intake, daily weight gain (DWG) and DM digestibility. DM consumption was different (P<span style="font-family:;" "=""> </span><span style="font-family:;" "="">< 0.01), the lowest consumption was 0.364 kg in treatment 1 (T1), with a maximum of 0.684 kg in T4;WDG was different (P</span><span style="font-family:;" "=""> </span><span style="font-family:;" "="">< 0.01), with a lower value of 0.044 kg·day<sup>-1</sup> in T1 and a higher value of 0.112 kg in T4. The apparent digestibility of the dry matter showed a difference (P</span><span style="font-family:;" "=""> </span><span style="font-family:;" "="">< 0.01) which varied from 45.52 to 59.15% for T1 and T4, respectively. DM intake, WL gain, and DM digestibility increased with increasing supplement level at the levels studied.</span>展开更多
In order to promote marker-assisted selection of Tan sheep, the polymorphism of OB gene in zan sheep was detected by sequencing and PCR-RFLP tech- niques. The correlation between polymorphism of OB gene and weight of ...In order to promote marker-assisted selection of Tan sheep, the polymorphism of OB gene in zan sheep was detected by sequencing and PCR-RFLP tech- niques. The correlation between polymorphism of OB gene and weight of Tan sheep was analyzed. The results indicated that there was a CT point mutation at locus 253 in exon 3 region of OB gene, but no mutant homozygote was found. The results of chi-square test showed that the frequency of BB and AB genotypes was con- sistent with Hardy-Weinberg equilibrium. AB genotype was the dominant genotype controlling weight of Tan sheep with the genotype frequency of 0.05. According to the results of correlation analysis, the mutation exerted significant effects on weight of Tan sheep at 3-month-old and 6-month-old （P 〈 0.05）. Moreover, interac- tions between ~enotvoe and ~ender had no sianificant effect on weight of Tan sheen （ P 〉 0.05 ）.展开更多
基金Supported by the National Nature Science Foundation of China(31460615)the Modern Agroindustry Technology Research System(CARS-39)
文摘The present experiment was conducted to determine the effects of Molasses-Urea Supplementation （MUS） on weight gain, ruminal fermentation and major microbial populations in sheep on a winter grazing regime in Inner Mongolia. Total 40 sheep, allowed free consumption of MUS after grazing, served as a treatment group, while 30 sheep, fed only by pasture grazing, served as a control group. Ruminal fermentation parameters, consisted of pH, Bacterial Crude Protein （BCP） and ammonia nitrogen （NH3-N） were measured. In addition, numbers of five symbiotic bacteria were investigated. The results showed as follows： the average daily weight gain, concentration of NH3-N and numbers of protozoa were significantly higher （p〈0.05） in the treatment group than those in the control group. Contrastingly, no significant difference was found in BCP concentration and pH between the two groups. At the end of the experiment, the populations of Selenomonas ruminantium, Anaerovibrio lipolytica, Fibrobacter succinogenes, Ruminococcus flaveciens and Ruminococcus albus in the treatment group were significantly higher than those of the control group （p〈0.05）. These results demonstrated that greater weight gain could be induced during winter in Inner Mongolia by improved nutritional status through promotion of microbial populations using urea and sugar.
文摘为了解脂尾绵羊的尾部性状特征,该试验选择滩羊典型生产区开展调查和试验观测,通过测量滩羊尾部尺寸、重量及尾骨特征,分析滩羊尾型特征及脂尾性状间的表型相关。结果显示:滩羊尾型可分为圆盘形尾、楔形尾和三角形尾3种;滩羊尾椎数量为17~21枚不等:根据尾椎椎骨的完整程度,滩羊的尾椎可分为4类,第1类尾椎的横突、棘突、椎弓、椎孔完整,第2类尾椎的横突完整,棘突、椎弓、椎孔退化,第3类尾椎的横突、棘突、椎弓、椎孔均发生退化,第4类尾椎椎骨之间融合,无关节囊。滩羊尾重与尾厚,尾脂率与尾重及尾脂重之间相关性显著。滩羊尾重与尾长、尾厚之间的最优回归方程为:y=-2.237+0.401 x 3+0.041 x 1(R 2=0.634,P<0.01)。
基金supported by Genetically Modified Organisms Breeding Major Projects ( 2009ZX08009-160B) Science and Technology Guidance Plan of Xinjiang Academy of Agricultural and Reclamation Sciences (YYD2010-8)+1 种基金 Science and Technology Key Project of Bintuan Agriculture (2009GG17,2011BA006) Doctoral Foundation of Bintuan (2011BB015)
文摘[ Objective] The study aimed to analyze the expression and polymorphism of KAP6.1 gene in various tissues of sheep, as well as the correlation between KAP6.1 gene and wool traits, so as to provide scientific references for the further study on the functions of KAP6.1 gene and molecular breeding of fine wool sheep. [Method] By using real-time fluorescence quantitative PCR, the expression map of KAP6.1 gene in various tissues was analyzed, and then PCR-SSCP technology, cloning and sequencing were adopted to analyze the polymorphism of KAP6.1 gene in 693 Chinese merino sheep （Xinjiang Junken type）, finally the correlation between KAPS. 1 gene and wool traits was discussed. [ Result] A high -level expression of KAP6.1 gene appeared in sheep skin, very significantly higher than that in muscle, small intestines, ovaries, hearts, lungs, livers, spleens, kidneys and rumen （ P 〈0.01 ）. In addition, there was C159T base substitution in KAF6.1 gene sequence, and analysis of the least squares model showed that the mean wool fleece weight of BB genotype at C159T site was significantly higher than that of AA and AB genotype （ P 〈0.05）, while there was no significant difference in average wool fiber diameter, curvature, length, clean fleece rate and density among hA, BB and AB genotype of KAP6.1 gene at C159T site （P〉0.05）. [Conclusion] KAP6.1 gene could be as the candidate gene of wool yield of sheep, and BB genotype could be used as the important molecular marker of fine wool sheep for high wool yield.
文摘Sheep meat production in the dry tropics of Mexico is low in the dry season, <span>due to lack of forage, therefore, strategic supplementation is important to av</span>oid weight loss in animals;The research was carried out from August 15 to De<span>cember 15, 2020 in order to evaluate the productive response of pelibuey sh</span>eep in confinement, fed with pangola grass hay (GH), plains grass silage (PGS) and supplemented with commercial feed (CF) in the dry season. 16 sheep of the same weight and age were used, four treatments were evaluated in them, in a completely random design;the treatments were four levels of supplement 0, 0.1, 0.2 and 0.3 kg of AC fed with HP and PGS. Sheep were dewormed at the beginning of the experiment and housed in individual cages. The variables were: dry matter intake, daily weight gain (DWG) and DM digestibility. DM consumption was different (P<span style="font-family:;" "=""> </span><span style="font-family:;" "="">< 0.01), the lowest consumption was 0.364 kg in treatment 1 (T1), with a maximum of 0.684 kg in T4;WDG was different (P</span><span style="font-family:;" "=""> </span><span style="font-family:;" "="">< 0.01), with a lower value of 0.044 kg·day<sup>-1</sup> in T1 and a higher value of 0.112 kg in T4. The apparent digestibility of the dry matter showed a difference (P</span><span style="font-family:;" "=""> </span><span style="font-family:;" "="">< 0.01) which varied from 45.52 to 59.15% for T1 and T4, respectively. DM intake, WL gain, and DM digestibility increased with increasing supplement level at the levels studied.</span>
基金Supported by Special Project of Dominant Characteristic Agriculture Breeding in Ningxia Hui Autonomous RegionMajor Project of Agriculture in Ningxia Hui Autonomous Region(2012ZDN1001)
文摘In order to promote marker-assisted selection of Tan sheep, the polymorphism of OB gene in zan sheep was detected by sequencing and PCR-RFLP tech- niques. The correlation between polymorphism of OB gene and weight of Tan sheep was analyzed. The results indicated that there was a CT point mutation at locus 253 in exon 3 region of OB gene, but no mutant homozygote was found. The results of chi-square test showed that the frequency of BB and AB genotypes was con- sistent with Hardy-Weinberg equilibrium. AB genotype was the dominant genotype controlling weight of Tan sheep with the genotype frequency of 0.05. According to the results of correlation analysis, the mutation exerted significant effects on weight of Tan sheep at 3-month-old and 6-month-old （P 〈 0.05）. Moreover, interac- tions between ~enotvoe and ~ender had no sianificant effect on weight of Tan sheen （ P 〉 0.05 ）.
