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Influence of High Temperature on the Expression of Arabidopsis Heat Shock Transcription Factor AtHsfA1a 被引量:1
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作者 Lihong GUO Juan CAI +1 位作者 Zhixuan YU Zhicuo DAN 《Agricultural Biotechnology》 CAS 2013年第1期13-15,共3页
[ Objective] This study ~med to investigate the influence of high temperature on the expression of heat shock transcription factor AtHsfAla in different genotypes of Arabidopsis. [ Method ] Arabidopsis plants overexpr... [ Objective] This study ~med to investigate the influence of high temperature on the expression of heat shock transcription factor AtHsfAla in different genotypes of Arabidopsis. [ Method ] Arabidopsis plants overexpressing heat shock transcription factor AtHsfA1 a were used as experimental materials and treated un- der high temperature at 39℃ for 1 rain and 5 min; total RNA of AtI-IsfAla was extracted, and the reverse transcription and amplification were conducted using RT- PCR technology, the amplification products were detected by electrophoresis. [ Result ] The expression levels of AtHsfA1 a in Arabidopsis plants overexpressing heat shock transcription factor AtHsfAla at high temperature and room temperature were higher than wild-type Arabidopsis; the expression levels of AtHsfAla in both wild-type Arab/dops/s and transgenic Arabidopsls plants overexpressing heat shock transcription factor AtHsfAla at high temperature of 39 ~C were higher than that at room temperature of 25 ~C, but the expression levels of AtHsfAla in wild-type Arab/dops/s and transgenic Arab/dops/s plants overexpressing heat shock transcription factor AtHsfAla varied little after high temperature treatment at 39 ~C for 1 rain or 5 rain. [ Conclusion] The expression of AtHsfAla is induced rapidly by high tem- perature, thus regulating the expression of early adversity-resistant genes. This study will lay the foundation demonstrating the mechanism of Arabidopsis heat shock transcription factor AtHsfAla. 展开更多
关键词 High temperature Arab/dops/s heat shock transcription factor AtHsfAla Gene expression
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Effects of extract F of red-rooted Salvia on mucosal lesions of gastric corpus and antrum induced by hemorrhagic shock-reperfusion in rats 被引量:5
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作者 Li-Hong Zhang~1 Chang-Bai Yao~2 He-Quan Li~3 1 Department of Anesthesiology,Second Clinical College,China Medical University,Shenyang 110003,Liaoning Province,China2 Department of General Surgery,Second Clinical College,China Medical University3 Department of Pathophysiology,China medical University 《World Journal of Gastroenterology》 SCIE CAS CSCD 2001年第5期672-677,共6页
AIM: To compare the effects of extract F of red-rooted Salvia (EFRRS) on mucosal lesions of gastric corpus and antrum induced by hemorrhagic shock and reperfusion in rats. METHODS: The rats were subject to hemorrhagic... AIM: To compare the effects of extract F of red-rooted Salvia (EFRRS) on mucosal lesions of gastric corpus and antrum induced by hemorrhagic shock and reperfusion in rats. METHODS: The rats were subject to hemorrhagic shock and followed by reperfusion, and were divided randomly into two groups. Group 1 received saline, and group 2 received EFRRS intravenously. The index of gastric mucosal lesions (IGML) was expressed as the percentage of lesional area in the corpus or antrum. The degree of gastric mucosal lesions (DGML) was catalogued grade 0,1,2 and 3. The concentrations of prostaglandins (PGs) were measured by radioimmunoassay. The concentration of MDA was measured according to the procedures of Asakawa. The activity of SOD was measured by the biochemical way. The growth rates or inhibitory rates of above-mentioned parameters were calculated. RESULTS: As compared with IGML (%), grade 3 damage (%) and MDA content (nmol/g tissue) of gastric antrum which were respectively 7.96 +/- 0.59, 34.86 +/- 4.96 and 156.98 +/- 16.12, those of gastric corpus which were respectively 23.18 +/- 6.82, 58.44 +/- 9.07 and 230.56 +/- 19.37 increased markedly (P 【0.01), whereas the grade 0 damage, grade 1 damage, the concentrations of PGE(2) and PGI(2)(pg/mg tissue), the ratio of PGI(2)/TXA(2) and the activity of SOD (U/g tissue) of corpus which were respectively 3.01 +/- 1.01, 8.35 +/- 1.95, 540.48 +/- 182.78, 714.38 +/- 123.74, 17.38 +/- 5.93 and 134.29 +/- 13.35 were markedly lower than those of antrum which were respectively 13.92 +/- 2.25, 26.78 +/- 6.06, 2218.56 +/- 433.12, 2531.76 +/- 492.35, 43.46 +/- 8.51 and 187.45 +/- 17.67 (P【0.01) after hemorrhagic shock and reperfusion.After intravenous EFRRS, the growth rates (%) of grade 0 damage, grade 1 damage, the concentrations of PGE(2) and PGI(2), the ratio of PGI(2)/TXA(2) and the activity of SOD of corpus which were respectively 632.56, 308.62, 40.75, 74.75, 92.29 and 122.25 were higher than those in antrum which were respectively 104.89, 58.40, 11.12, 56.58, 30.65 and 82.64, whereas the inhibitory rates (%) of IGML, grade 3 damage and MDA content of gastric corpus were 82.93, 65.32 and 59.09, being higher than those of gastric antrum which were 76.64, 53.18 and 42.37. CONCLUSION: After hemorrhagic shock reperfusion, the gastric mucosal lesions in the corpus were more severe than those in the antrum, which were related not only to the different distribution of endogenous PGs in the mucosa, but also to the different ability of anti-oxidation of the mucosa. The protective effect of EFRRS on the gastric mucosa in the corpus was more evident than that in the antrum, which was related to higher growth degree of PGs contents and anti-oxitative ability in gastric corpus after administration of EFRRS. 展开更多
关键词 PHYTOTHERAPY sALVIA ALPROsTADIL derivatives Animals DINOPROsTONE Gastric Mucosa Hydroxyl Radical Male Malondialdehyde Plant Extracts Pyloric Antrum RATs Rats Wistar Reperfusion Injury Research support Non-U.s. Gov't shock Hemorrhagic superoxide Dismutase Thromboxane B2
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Method discussion for quick response grey prediction of stronger aftershocks of an earthquake sequence
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作者 平建军 李永庆 张清荣 《Acta Seismologica Sinica(English Edition)》 CSCD 1999年第1期78-83,共6页
In this paper, we take occurrence process of early strong aftershocks of a main after shock type′s earthquake sequence as a complex grey system, and introduce predicting method for its stronger aftershocks by grey p... In this paper, we take occurrence process of early strong aftershocks of a main after shock type′s earthquake sequence as a complex grey system, and introduce predicting method for its stronger aftershocks by grey predicting theory. Through inspection prediction for 1998 Zhangbei M S=6.2 earthquake sequence, it shows that the grey predicting method maybe has active significance for the investigation of quick response prediction problems of stronger aftershocks of an earthquake sequence. 展开更多
关键词 MAIN after shock type′s earthquake sequence stronger aftershock grey prediction
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Eco-mindfulness in Buddhism
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作者 Kenneth Lee 《Journal of Literature and Art Studies》 2017年第12期1645-1650,共6页
Many of those involved in the ecological movement have found inspiration and parallels within the Buddhist tradition. The Buddhist philosophy of karmic causality and dependent origination (pratitya-samutpada) convey... Many of those involved in the ecological movement have found inspiration and parallels within the Buddhist tradition. The Buddhist philosophy of karmic causality and dependent origination (pratitya-samutpada) convey the notion of interdependence of humans and nature. Today, especially in the United States, there are many movements, such as the Buddhist Peace Fellowship, to promote eco-living and sustainable communities, which call for humans to be mindful of the environment and make conscientious choices for the benefit of all sentient beings and non-sentient entities. Just as humans have been the cause of the gradual deterioration of Nature, we can also be the solution towards an coo-conscious living, which not only fosters a healthy environment, but also enables us to build good merit. The core Buddhist teaching, which must be embraced by all eco-minded practitioners is the notion of interdependence, which has been expounded by notable Japanese Buddhist figures in the eighth century, such as Kukai (774-835) of the Shingon School and Dogen (1200-1253) of the Soto Zen sect, who both viewed that non-living life forms--trees, plants, and the earth--could also achieve enlightenment. Their view is based on the ontological notions of Buddha-nature (tathagata-garbha) and Indra's Jewel Net, which is a powerful image found in Huayen's (Jpn. Kegon) Avatamsaka Sutra. This paper examines Buddhist teachings and sutras, which support eco-friendly and sustainable movements in our society and world today. 展开更多
关键词 mindFULNEss BUDDHIsM nature environment dependent origination Eco-living Indra's Net
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Heat shock protein 90 is a potential therapeutic target for ameliorating skeletal muscle abnormalities in Parkinson's disease
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作者 Nour Erekat Ahed Al-Khatib Muhammed Al-Jarrah 《Neural Regeneration Research》 SCIE CAS CSCD 2014年第6期616-621,共6页
Previous studies have confirmed that heat shock protein 90 overexpression can lead to dopami- nergic neuronal death. This study was designed to further investigate what effects are produced by heat shock protein 90 af... Previous studies have confirmed that heat shock protein 90 overexpression can lead to dopami- nergic neuronal death. This study was designed to further investigate what effects are produced by heat shock protein 90 after endurance exercise training. Immunohistochemistry results showed that exercise training significantly inhibited heat shock protein 90 overexpression in the soleus and gastrocnemius in Parkinson's disease rats, which is a potential therapeutic target for ameliorating skeletal muscle abnormalities in Parkinso^s disease. 展开更多
关键词 nerve regeneration heat shock protein 90 Parkinson's disease exerecise soleus muscle gastrocnemius muscle skeletal muscle treadmill excise neural regeneration
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The state of the Mind in a Revolutionary state of the State
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作者 Nditsheni Emmanuel Tshikwatamba 《Sociology Study》 2014年第1期69-84,共16页
The article epitomises its foundations by providing definitional and explanative dichotomy among the State, Nation, and Nation State. It affords a depiction of the centrality of the mind, the interface of the body and... The article epitomises its foundations by providing definitional and explanative dichotomy among the State, Nation, and Nation State. It affords a depiction of the centrality of the mind, the interface of the body and mind in behavioural manifestations. It furthermore acknowledges that nothing occurs in a vacuum but in the context of the fullness of the mind. The visible manifestations of human behaviour are contended to be informed by the noiselessness invisible aspects of the mind. The same noiseless mind that informs the behavioural visibility that becomes somewhat active and busier in its conspicuousness. The dual existence of things and events in a revolutionary context that transcends to other behavioural undertones is discoursed. In the context application of the revolutionary mapping, the mind is presented as a culprit revolutionarist that wages revolutions without fear of external threats through arrests, danger, pain, terror, dread, apprehension, detentions, and any other similar means including death. The article settles that the mind is the determinant factor of human behaviours and it therefore defines the state of the State in any revolutionary circumstance. It denotes a functional separatism of the mind from the brain which is an organ of the body thereby equally accepting that the brain functionalises the mind. The equal essential role of the mind in post revolution construction to establish and promote an acceptable system of government that responds to socio-economic factors is detailed herein the manuscript. A deliberate attempt is made not to be suggestive and prescriptive on the shape and form of such a post revolution system of government. 展开更多
关键词 Revolutionary mind DUALIsM state(s CONsTRUCTs
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A Review on the Evolution and Development of Searle' s Philosophy of Mind
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作者 Zhao Yingji 《学术界》 CSSCI 北大核心 2013年第12期291-299,共9页
The evolution and development of Searle’s philosophy of mind can be divided into two important stages,i.e.the origin and beginning stage between 1960sand 1970s,the formation and development stage since 1980s.Searle’... The evolution and development of Searle’s philosophy of mind can be divided into two important stages,i.e.the origin and beginning stage between 1960sand 1970s,the formation and development stage since 1980s.Searle’s Speech Act Theory started the research on philosophy of mind,which is considered as the origin of his philosophy of mind.The Theories of Intentionality and Chinese Room Argument are the most important and most influential academic findings in his research in the field of philosophy of mind,which lay a solid theoretical foundation for the formation and development of his theories of philosophy of mind.Searle’s philosophy of mind finds wide prospect in the research field,which provides a new direction to understand the world and probe into mind,proposes a new perspective and methodology for the study of language,and promotes the new development of the study of language. 展开更多
关键词 心灵哲学 塞尔 述评 演进 心境 言语行为理论 学术成果 意向性
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Modeling and Analysis of Electrostatically Actuated MEMS under Combined Nonlinearities, Lorentz Force, and Mechanical Shock
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作者 Emran Khoshrouye Ghiasi 《Journal of Control Science and Engineering》 2016年第2期82-89,共8页
This paper shows an analysis ofMEM S (micro electro mechanical systems) due to Lorentz force and mechanical shock. The formulation is based on a modified couple stress theory, the von Karman geometric nonlinearity a... This paper shows an analysis ofMEM S (micro electro mechanical systems) due to Lorentz force and mechanical shock. The formulation is based on a modified couple stress theory, the von Karman geometric nonlinearity and Reynolds equation as well. The model contains a silicon microbeam, which is encircled by a stationary plate. The non-dimensional governing equations and associated boundary conditions are then solved iteratively through the Galerkin weighted method. The results show that pull-in voltage is dependent on the geometry nonlinearity. It is also demonstrated that by increasing voltage between the silicon microbeam and stationary plate, the pull-in instability happens. 展开更多
关键词 M EM s mechanical shock NONLINEARITY PULL-IN Galerkin weighted method dimensionless parameters.
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Nature's Influence on Children: Wordsworth's Concern with Children's Mind
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作者 王晓东 《海外英语》 2010年第8X期205-208,共4页
Romanticism is an important stage in the evolution of man's self-consciousness. It shows new interest in and new concern with man and nature. Wordsworth is well-known as a nature poet. The theme of human mind and ... Romanticism is an important stage in the evolution of man's self-consciousness. It shows new interest in and new concern with man and nature. Wordsworth is well-known as a nature poet. The theme of human mind and nature in his poems has long attracted readers' attention. However, he does not indulge in nature itself but takes it as consolation and inspiration for human kind. In order to highlight the poet's concern with human mind, the present paper chooses to explore Wordsworth's concern with children's mind, based on the analysis of Wordsworth's The Prelude and many of his poems about children. 展开更多
关键词 ROMANTICIsM WORDsWORTH children’s mind
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The First Emperor's Mausoleum Shocks the World Again
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《China & The World Cultural Exchange》 2000年第1期45-46,共2页
关键词 EMPEROR World The First Emperor’s Mausoleum shocks the World Again
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Accounting for Quadratic and Cubic Invariants in Continuum Mechanics–An Overview
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作者 Artur V.Dmitrenko Vladislav M.Ovsyannikov 《Fluid Dynamics & Materials Processing》 EI 2024年第9期1925-1939,共15页
The differential equations of continuum mechanics are the basis of an uncountable variety of phenomena and technological processes in fluid-dynamics and related fields.These equations contain derivatives of the first ... The differential equations of continuum mechanics are the basis of an uncountable variety of phenomena and technological processes in fluid-dynamics and related fields.These equations contain derivatives of the first order with respect to time.The derivation of the equations of continuum mechanics uses the limit transitions of the tendency of the volume increment and the time increment to zero.Derivatives are used to derive the wave equation.The differential wave equation is second order in time.Therefore,increments of volume and increments of time in continuum mechanics should be considered as small but finite quantities for problems of wave formation.This is important for calculating the generation of sound waves and water hammer waves.Therefore,the Euler continuity equation with finite time increments is of interest.The finiteness of the time increment makes it possible to take into account the quadratic and cubic invariants of the strain rate tensor.This is a new branch in hydrodynamics.Quadratic and cubic invariants will be used in differential wave equations of the second and third order in time. 展开更多
关键词 Quadratic invariant cubic invariant continuity equation generation of periodic waves N.E.Zhukovsky’s hydraulic shock TURBULENCE
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基于L-S理论的压电球壳广义热冲击分析 被引量:4
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作者 刘承斌 刘文耀 +2 位作者 陈伟球 王惠明 吕朝锋 《浙江大学学报(工学版)》 EI CAS CSCD 北大核心 2018年第6期1185-1193,共9页
基于L-S广义热弹性理论,针对压电球壳受表面球对称热冲击作用下的热弹性问题进行分析.从三维压电弹性理论基本方程出发,利用Laplace变换,建立耦合的6阶状态方程;采用层合近似模型,将状态方程转化为关于径向坐标的常系数状态方程.利用界... 基于L-S广义热弹性理论,针对压电球壳受表面球对称热冲击作用下的热弹性问题进行分析.从三维压电弹性理论基本方程出发,利用Laplace变换,建立耦合的6阶状态方程;采用层合近似模型,将状态方程转化为关于径向坐标的常系数状态方程.利用界面连续条件得到球壳内、外状态变量的整体传递关系,结合球壳内、外边界条件,可以确定频域内所有状态变量,通过数值反变换获得时域解.数值算例给出热冲击作用下压电球壳的位移、应力、电势和温度等物理量的分布规律,考察热松弛时间对热冲击作用效果的影响. 展开更多
关键词 L-s广义热弹性 压电球壳 LAPLACE变换 状态空间法 热冲击
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唐山地区近震S波分裂的观测与研究 被引量:4
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作者 高原 冯德益 《地震》 CSCD 北大核心 1995年第3期208-213,共6页
文中主要采用Stuart Crampin提出的偏振分析技术,把数字化的地震资料进行了滤波和还原处理,对唐山地区进行了近震S波分裂的分析,得出这一地区的主压应力场方向为北东方向。
关键词 s波分裂 近震 偏振 时间延迟 地震波 唐山市
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环磷酰胺激活小鼠肝微粒体谷胱甘肽S-转移酶Ⅰ的机制研究 被引量:2
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作者 郑英 邹晓华 +3 位作者 付再林 方慧 陈素红 吕圭源 《中国药理学通报》 CAS CSCD 北大核心 2013年第10期1387-1390,共4页
目的探讨环磷酰胺(CP)在体内对小鼠肝微粒体谷胱甘肽S-转移酶Ⅰ(mGST-Ⅰ)活性的影响及其可能的机制。方法用苯巴比妥75 mg·kg-1诱导小鼠CYP2B后,ip CP,测定9 h内mGST-Ⅰ酶活性,观察二巯基丁二醇(DTT)对酶激活的逆转作用和巯基烷化... 目的探讨环磷酰胺(CP)在体内对小鼠肝微粒体谷胱甘肽S-转移酶Ⅰ(mGST-Ⅰ)活性的影响及其可能的机制。方法用苯巴比妥75 mg·kg-1诱导小鼠CYP2B后,ip CP,测定9 h内mGST-Ⅰ酶活性,观察二巯基丁二醇(DTT)对酶激活的逆转作用和巯基烷化剂N-乙基马来酰亚胺(NEM)对酶再激活效应,用SDS-PAGE和负染凝胶法评价CP对mGST-Ⅰ蛋白表达的影响。结果 CP引起小鼠微粒体中mGST-Ⅰ活性增加;NEM在mGST-Ⅰ半胱氨酸-49-巯基(cys-49-SH)上的活化效应减弱,而CP引起的mGST-Ⅰ激活效应不被二硫键还原剂DTT逆转。激活的mGST-Ⅰ电泳图谱未见蛋白分子量变迁及蛋白表达增加。结论大剂量CP致mGST-Ⅰ激活效应机制主要是酶分子cys-49-SH上单个巯基的修饰作用。 展开更多
关键词 环磷酰胺 微粒体 谷胱甘肽s-转移酶Ⅰ 二巯基丁二醇 N-乙基马来酰亚胺 半胱氨酸-49-巯基 机制
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HSP27和GST在大肠腺瘤和大肠癌中表达的临床病理研究 被引量:5
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作者 邓亚平 曾亮 +3 位作者 陈建军 刘志红 陈森林 胡云桂 《肿瘤基础与临床》 2006年第1期7-9,共3页
目的探讨热休克蛋白27(HSP27)和谷胱甘肽-S-转移酶(GST)在正常大肠黏膜、大肠腺瘤和大肠癌中的表达及其临床病理意义。方法应用免疫组织化学S-P法检测HSP27和GST在大肠组织中的表达。结果HSP27在正常肠黏膜、大肠腺瘤、无淋巴结转移的... 目的探讨热休克蛋白27(HSP27)和谷胱甘肽-S-转移酶(GST)在正常大肠黏膜、大肠腺瘤和大肠癌中的表达及其临床病理意义。方法应用免疫组织化学S-P法检测HSP27和GST在大肠组织中的表达。结果HSP27在正常肠黏膜、大肠腺瘤、无淋巴结转移的大肠癌、有淋巴结转移的大肠癌中表达阳性率分别为30%,33.3%,65%,70%,多组间比较具有显著性差异(χ2=12.723,P=0.013)。两组间比较发现,HSP27表达率在正常组与大肠癌组存在差异,在有淋巴结转移的大肠癌组中的表达具有差异最为显著(χ2=8.688,P=0.003),而其它组间比较无显著性差异。GST在正常肠黏膜、大肠腺瘤、无淋巴结转移的大肠癌、有淋巴结转移的大肠癌中表达阳性率分别为60%,80.9%,90%,100%,多组间比较具有显著性差异(χ2=16.707,P=0.001);进一步进行两组间比较发现,GST表达率在有淋巴结转移的大肠癌组中的表达具有差异最为显著(χ2=10.909,P=0.001),而其它组间比较无显著性差异。GST和HSP27表达无显著相关。结论HSP27和GST在大肠癌中的表达可能在大肠癌发生发展中发挥作用,特别在大肠癌恶性演进中以及预后预测中具有重要意义,可能作为独立的生物标志物。 展开更多
关键词 大肠癌 热休克蛋白27 谷胱甘肽s转移酶
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金黄色葡萄球菌GST-mTSST-1的免疫活性检测方法的研究 被引量:2
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作者 崔京春 郭海勇 +4 位作者 孙传丽 屈嫄 于鑫 常颖 范圣第 《大连民族学院学报》 CAS 2005年第3期51-53,共3页
通过对影响琼脂扩散反应的多种条件进行优化,建立了检测金黄色葡萄球菌无毒诱变中毒性休克综合征毒素1融合蛋白GST-mTSST-1免疫活性的双向免疫琼脂扩散检测方法.检测的灵敏度可达到120ng/mL.此方法具有特异性强、灵敏度高、操作简单等特... 通过对影响琼脂扩散反应的多种条件进行优化,建立了检测金黄色葡萄球菌无毒诱变中毒性休克综合征毒素1融合蛋白GST-mTSST-1免疫活性的双向免疫琼脂扩散检测方法.检测的灵敏度可达到120ng/mL.此方法具有特异性强、灵敏度高、操作简单等特点,对具有免疫活性的抗原蛋白的定量检测有一定的参考价值. 展开更多
关键词 金黄色葡萄球菌 GsT-mTssT-1 免疫活性 检测
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S-亚硝基谷胱甘肽对舱室内大鼠颅脑爆炸伤后继发性脑损伤的作用 被引量:2
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作者 许明伟 许民辉 +3 位作者 赖西南 王丽丽 张子焕 崔红 《创伤外科杂志》 2010年第6期491-495,共5页
目的观察S-亚硝基谷胱甘肽(GSNO)在舱室内大鼠颅脑爆炸伤后继发性脑损伤中的作用,探讨其在舱室颅脑爆炸伤防治中的临床应用价值。方法 88只SD大鼠完全随机分为正常对照组(n=8)、单纯致伤组(n=40)和致伤+GSNO治疗组(n=40);采用二硝基重氮... 目的观察S-亚硝基谷胱甘肽(GSNO)在舱室内大鼠颅脑爆炸伤后继发性脑损伤中的作用,探讨其在舱室颅脑爆炸伤防治中的临床应用价值。方法 88只SD大鼠完全随机分为正常对照组(n=8)、单纯致伤组(n=40)和致伤+GSNO治疗组(n=40);采用二硝基重氮酚(DDNP)纸质点爆源在模拟装甲舱室爆炸,建立颅脑爆炸伤模型。伤后1、6、12、24、48小时取标本。测定脑组织中肿瘤坏子因子-α(TNF-α)、白介素-1β(IL-1β)浓度;丙二醛(MDA)浓度及超氧化物岐化酶(SOD)活性;观察脑组织病理学变化。结果致伤组伤后1小时脑组织TNFα-、IL-1β、MDA浓度均明显升高(P<0.01),伤后12小时升高达峰值(P<0.01),伤后48小时脑组织TNF-α、IL-1β浓度仍显著高于正常对照组(P<0.01)。致伤组伤后1小时脑组织SOD活性即显著降低(P<0.01),致伤后12小时降低至最低值(P<0.01),伤后48小时仍低于正常值。致伤组脑血管内皮细胞肿胀、脑水肿、神经元变性、坏死明显;给予GSNO治疗后各时间点脑组织TNF-α、IL-1β、MDA浓度均较致伤组明显降低,SOD活性明显增高(P<0.01或P<0.05)。治疗组脑水肿、神经元变性、坏死等表现均较单纯致伤组轻。结论舱室颅脑爆炸伤后可导致大鼠严重的继发性脑损伤;而早期给予GSNO可明显减轻脑组织炎性损伤和氧化损伤,减轻脑水肿及神经元变性、坏死,从而减轻继发性脑损伤。 展开更多
关键词 颅脑损伤 爆炸伤 冲击波 s-亚硝基谷胱甘肽
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葡萄球菌GST-mTSST-1融合蛋白抗体间接ELISA检测方法的建立 被引量:2
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作者 郭海勇 崔京春 钱爱东 《中国兽医科学》 CAS CSCD 北大核心 2007年第12期1041-1045,共5页
以纯化的金黄色葡萄球菌无毒诱变超抗原GST-mTSST-1融合蛋白作为检测抗原,通过优化ELISA反应条件,初步建立了检测血清特异性GST-mTSST-1抗体的间接ELISA方法,监测了小鼠血清中特异性GST-mTSST-1抗体水平的动态变化规律。方阵试验确定的G... 以纯化的金黄色葡萄球菌无毒诱变超抗原GST-mTSST-1融合蛋白作为检测抗原,通过优化ELISA反应条件,初步建立了检测血清特异性GST-mTSST-1抗体的间接ELISA方法,监测了小鼠血清中特异性GST-mTSST-1抗体水平的动态变化规律。方阵试验确定的GST-mTSST-1抗原的最适包被浓度为5.0μg/mL,血清最佳稀释倍数为1∶100,ELISA阳性反应的临界值为D490 nm≥0.219,批内和批间重复试验的变异系数均小于10%。应用该方法对试验小鼠血清进行检测,结果表明,建立的间接ELISA方法具有较高的敏感性、特异性和较好的重复性,适用于检测特异性GST-mTSST-1血清抗体。 展开更多
关键词 金黄色葡萄球菌 GsT-mTssT-1 融合蛋白 间接ELIsA 超抗原
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热锻炼能提高菜豆细胞可溶性蛋白、SOD、膜ATP酶和质子泵在体外的热稳定性 被引量:5
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作者 吴显荣 刘箭 徐晓玲 《生物化学杂志》 CSCD 1995年第3期259-263,共5页
以菜豆为实验材料,研究了蛋白质分子和生物膜在体外热稳定性的变化和原因。实验结果表明:热锻炼可增加细胞的耐热性,并赋予SOD、膜ATP酶和生物膜质子泵在体外的热稳定性;通过对可溶性蛋白热变性后的浊度分析和用ANS荧光探... 以菜豆为实验材料,研究了蛋白质分子和生物膜在体外热稳定性的变化和原因。实验结果表明:热锻炼可增加细胞的耐热性,并赋予SOD、膜ATP酶和生物膜质子泵在体外的热稳定性;通过对可溶性蛋白热变性后的浊度分析和用ANS荧光探针探测蛋白质的结构变化,发现蛋白热变性的原因是疏水区的暴露和分子相互团聚,^(35)S-Met标记分析和二维电泳谱揭示,热锻炼诱导合成的热激蛋白具有阻止蛋白质热变性和生物膜热破坏的功能。 展开更多
关键词 热激蛋白 ATP酶 热锻炼 菜豆 超氧化物歧化酶
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渗透休克法从大肠杆菌中提取GST-Tα1-TP5融合蛋白 被引量:3
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作者 谢琦 刘永明 《华夏医学》 CAS 2016年第4期9-12,共4页
目的:从大肠杆菌中选择性抽提GST-Tα1-TP5融合蛋白。方法:采用渗透休克法从大肠杆菌中提取GST-Tα1-TP5融合蛋白,优化操作条件,并与超声破碎法进行比较。结果:优化后的渗透休克法从大肠杆菌提取GST-Tα1-TP5融合蛋白可使杂蛋白量含量... 目的:从大肠杆菌中选择性抽提GST-Tα1-TP5融合蛋白。方法:采用渗透休克法从大肠杆菌中提取GST-Tα1-TP5融合蛋白,优化操作条件,并与超声破碎法进行比较。结果:优化后的渗透休克法从大肠杆菌提取GST-Tα1-TP5融合蛋白可使杂蛋白量含量相对超声破碎法降低一个数量级。结论:渗透休克法可高效提取大肠杆菌表达的GST-Tα1-TP5融合蛋白。 展开更多
关键词 渗透休克法 大肠杆菌 谷胱甘肽硫转移酶 重组胸腺肽Tα1-TP5
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