The ultracytochemical effects in the liver of rabbit undergoing high energy shock wave (HESW) were studied with electron microscope. The application of lanthanum as a tracer for ultrastructural study demonstrated that...The ultracytochemical effects in the liver of rabbit undergoing high energy shock wave (HESW) were studied with electron microscope. The application of lanthanum as a tracer for ultrastructural study demonstrated that intracellular lanthanum could be observed, most of which entered the hepatocytes and its mitochondria. The lanthanum granules were also found to deposite in the zone of tight junctions of bile canaliculi, which indicated that the tight junctions had been damaged. The activities of succinate dehydrogenase (SDH) in liver cells, alkaline phosphatase (ALP) and thiamine pyrophosphatase (TPPase) on the wall of bile canaliculi became diminished obviously. Both the activites and localizations of TPPase had changed. Some TPPase from the damaged lysosome like vesicles and Golgi saccules of liver cells discharged into cytoplasm. TPPase reaction production in some bile canaliculi decreased. In the intercellular space of the liver cells and the tight junctions of bile canaliculi TPPase reaction could be seen. Ultrastructurally, the changes commonly seen were hydropic mitochondria and dilatation of rough endoplasmic reticulum. Serologic test demonstrated that there was an abnormal change of SGPT, SGOT and ALP. The results showed that HESW can damage the ultrastructure and function of liver.展开更多
The slit-type energy dissipater（STED）is widely used in hydraulic projects of high water head,large discharge,and narrow river valley,thanks to its simple structure and high efficiency.However,the water wing caused b...The slit-type energy dissipater（STED）is widely used in hydraulic projects of high water head,large discharge,and narrow river valley,thanks to its simple structure and high efficiency.However,the water wing caused by the shock waves in the contraction section of the STED may bring about harmful effects.A coefficient is introduced for the application of Ippen？s theory in the STED.The expression of the coefficient is experimentally obtained.Simplified formulas to calculate the shock wave angle and the water wing scope are theoretically derived,with relative errors within 5%.展开更多
文摘The ultracytochemical effects in the liver of rabbit undergoing high energy shock wave (HESW) were studied with electron microscope. The application of lanthanum as a tracer for ultrastructural study demonstrated that intracellular lanthanum could be observed, most of which entered the hepatocytes and its mitochondria. The lanthanum granules were also found to deposite in the zone of tight junctions of bile canaliculi, which indicated that the tight junctions had been damaged. The activities of succinate dehydrogenase (SDH) in liver cells, alkaline phosphatase (ALP) and thiamine pyrophosphatase (TPPase) on the wall of bile canaliculi became diminished obviously. Both the activites and localizations of TPPase had changed. Some TPPase from the damaged lysosome like vesicles and Golgi saccules of liver cells discharged into cytoplasm. TPPase reaction production in some bile canaliculi decreased. In the intercellular space of the liver cells and the tight junctions of bile canaliculi TPPase reaction could be seen. Ultrastructurally, the changes commonly seen were hydropic mitochondria and dilatation of rough endoplasmic reticulum. Serologic test demonstrated that there was an abnormal change of SGPT, SGOT and ALP. The results showed that HESW can damage the ultrastructure and function of liver.
基金supported by the National Nature Science Foundation of China(Grant Nos.51279013,51379020 and 51509015)the National Key R&D Program of China(Grant No.2016YFC0401900)
文摘The slit-type energy dissipater（STED）is widely used in hydraulic projects of high water head,large discharge,and narrow river valley,thanks to its simple structure and high efficiency.However,the water wing caused by the shock waves in the contraction section of the STED may bring about harmful effects.A coefficient is introduced for the application of Ippen？s theory in the STED.The expression of the coefficient is experimentally obtained.Simplified formulas to calculate the shock wave angle and the water wing scope are theoretically derived,with relative errors within 5%.
