We developed a system for the regeneration of Lombardy poplar (Populus nigra L. var. italica) shoots from internodal stem explants. Using this system, shoots regenerated from 87% of the stem explants placed on Murashi...We developed a system for the regeneration of Lombardy poplar (Populus nigra L. var. italica) shoots from internodal stem explants. Using this system, shoots regenerated from 87% of the stem explants placed on Murashige and Skoog (MS) medium supplemented with 0.1 mg/L indole-3-acetic acid and 0.5 mg/L benzylaminopurine without undergoing callus formation. About 80% of the in vitro regenerated shoots developed roots on MS medium supplemented with 0.5 mg/L indole-3-butyric acid and 0.02 mg/L 1-naphthylacetic acid. Well-rooted seven-to eight-week-old regenerated plants could be transferred to soil for further growth and the survival rate of such plants after three weeks was 88%. The protocol presented here is simple and economical because it does not rely on pre-incubation in callus induction medium or repeated subculture in shoot induction medium containing trans-zeatin, an expensive substance. The in vitro regeneration system presented here could be used for evaluation of radiation sensitivity for Lombardy poplar tissues.展开更多
The overwinter terminal buds of Paulownia elongata seedlings,tranplanted in February or before the mid of March in Zhengzhou of Henan Province were not able to flush due to water lost from the buds,and the bud died fr...The overwinter terminal buds of Paulownia elongata seedlings,tranplanted in February or before the mid of March in Zhengzhou of Henan Province were not able to flush due to water lost from the buds,and the bud died from the dry within 15～20 days after transplantation. Supplementary measures would be used to postpone flushing of terminal buds without influencing survival of the seedlings. By burying the seedlings in soil to the last ten days of March or the first ten days of April,the flush rate of the buds was able to be promoted. The rate of height of stem extension by terminal buds in height of main stem was 96.71% in the same year,and the rate of trunk straight by main stem extension of terminal buds was 100%. The figure of main stem extension process of terminal buds of Paulownia elongata seedling trees was given in this paper for the first time.展开更多
文摘We developed a system for the regeneration of Lombardy poplar (Populus nigra L. var. italica) shoots from internodal stem explants. Using this system, shoots regenerated from 87% of the stem explants placed on Murashige and Skoog (MS) medium supplemented with 0.1 mg/L indole-3-acetic acid and 0.5 mg/L benzylaminopurine without undergoing callus formation. About 80% of the in vitro regenerated shoots developed roots on MS medium supplemented with 0.5 mg/L indole-3-butyric acid and 0.02 mg/L 1-naphthylacetic acid. Well-rooted seven-to eight-week-old regenerated plants could be transferred to soil for further growth and the survival rate of such plants after three weeks was 88%. The protocol presented here is simple and economical because it does not rely on pre-incubation in callus induction medium or repeated subculture in shoot induction medium containing trans-zeatin, an expensive substance. The in vitro regeneration system presented here could be used for evaluation of radiation sensitivity for Lombardy poplar tissues.
文摘The overwinter terminal buds of Paulownia elongata seedlings,tranplanted in February or before the mid of March in Zhengzhou of Henan Province were not able to flush due to water lost from the buds,and the bud died from the dry within 15～20 days after transplantation. Supplementary measures would be used to postpone flushing of terminal buds without influencing survival of the seedlings. By burying the seedlings in soil to the last ten days of March or the first ten days of April,the flush rate of the buds was able to be promoted. The rate of height of stem extension by terminal buds in height of main stem was 96.71% in the same year,and the rate of trunk straight by main stem extension of terminal buds was 100%. The figure of main stem extension process of terminal buds of Paulownia elongata seedling trees was given in this paper for the first time.
