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Utilizing Short Tandem Repeats (STRs) as a Resolving Matrix in Parental Dispute DNA Analysis
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作者 George Gborienemi Simeon Alade Tolulope Olukemi 《American Journal of Molecular Biology》 2018年第3期156-165,共10页
Interest in DNA analysis using short tandem repeats (STR) as finger printing tools in forensic medicine has gained tremendous application, as expression of these nuclear factors have enhanced forensic examination. Her... Interest in DNA analysis using short tandem repeats (STR) as finger printing tools in forensic medicine has gained tremendous application, as expression of these nuclear factors have enhanced forensic examination. Here we used this Biochemical characterization after conventional extraction process, polymerase chain reaction (PCR), gel electrophoresiss and a sequencer to distinguish and resolve parental dispute. The differential migration of labeled DNA fragments which attains excitation energy with a laser elicits fluorescent light of different wavelength depending on the dye used. A data collection software (Genemapper) collects raw data (spectrograph) and converts it to an electropherogram that is interpreted. By comparing the DNA profiles, inclusion and exclusion criteria were elucidated to resolve disputes. The inherent discriminating power of STRs used in analysis enhances resolution of cell mixtures, genetic aberration, substantiation of tissue origin and provides genetic distinction which is a robust and reliable approach in resolving parental disputes. 展开更多
关键词 short tandem repeats Matrix PARENTAL DNA Analysis
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Identification and characterization of short tandem repeats in the Tibetan macaque genome based on resequencing data 被引量:1
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作者 San-Xu Liu Wei Hou +4 位作者 Xue-Yan Zhang Chang-Jun Peng Bi-Song Yue Zhen-Xin Fan Jing Li 《Zoological Research》 SCIE CAS CSCD 2018年第4期291-300,共10页
The Tibetan macaque, which is endemic to China, is currently listed as a Near Endangered primate species by the International Union for Conservation of Nature (IUCN)(2017). Short tandem repeats (STRs) refer to r... The Tibetan macaque, which is endemic to China, is currently listed as a Near Endangered primate species by the International Union for Conservation of Nature (IUCN)(2017). Short tandem repeats (STRs) refer to repetitive elements of genome sequence that range in length from 1-6 bp. They are found in many organisms and are widely applied in population genetic studies. To clarify the distribution characteristics of genome-wide STRs and understand their variation among Tibetan macaques, we conducted a genome-wide survey of STRs with next-generation sequencing of five macaque samples. A total of 1 077 790 perfect STRs were mined from our assembly, with an N50 of 4 966 bp. Mono-nucleotide repeats were the most abundant, followed by tetra- and di-nucleotide repeats. Analysis of GC content and repeats showed consistent results with other macaques. Furthermore, using STR analysis software (IobSTR), we found that the proportion of base pair deletions in the STRs was greater than that of insertions in the five Tibetan macaque individuals (P〈0.05, t-test). We also found a greater number of homozygous STRs than heterozygous STRs (P〈0.05, t-test), with the Emei and Jianyang Tibetan macaques showing more heterozygous loci than Huangshan Tibetan macaques. The proportion of insertions and mean variation of alleles in the Emei and Jianyang individuals were slightly higher than those in the Huangshan individuals, thus revealing differences in STR allele size between the two populations The polymorphic STR loci identified based on the reference genome showed good amplification efficiency and could be used to study population genetics in Tibetan macaques. The neighbor-joining tree classified the five macaques into two different branches according to their geographical origin, indicating high genetic differentiation between the Huangshan and Sichuan populations. We elucidated the distribution characteristics of STRs in the Tibetan macaque genome and provided an effective method for screening polymorphic STRs. Our results also lay a foundation for future genetic variation studies of macaques. 展开更多
关键词 Tibetan macaque (Macaca thibetana) genome short tandem repeats Variation analysis POLYMORPHISM Next-generation sequencing
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Mapping short tandem repeats for liver gene expression traits helps prioritize potential causal variants for complex traits in pigs
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作者 Zhongzi Wu Huanfa Gong +6 位作者 Zhimin Zhou Tao Jiang Ziqi Lin Jing Li Shijun Xiao Bin Yang Lusheng Huang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2022年第3期707-720,共14页
Background:Short tandem repeats(STRs)were recently found to have significant impacts on gene expression and diseases in humans,but their roles on gene expression and complex traits in pigs remain unexplored.This study... Background:Short tandem repeats(STRs)were recently found to have significant impacts on gene expression and diseases in humans,but their roles on gene expression and complex traits in pigs remain unexplored.This study investigates the effects of STRs on gene expression in liver tissues based on the whole-genome sequences and RNA-Seq data of a discovery cohort of 260 F6 individuals and a validation population of 296 F7 individuals from a heterogeneous population generated from crosses among eight pig breeds.Results:We identified 5203 and 5868 significantly expression STRs(eSTRs,FDR<1%)in the F6 and F7 populations,respectively,most of which could be reciprocally validated(π1=0.92).The eSTRs explained 27.5%of the cisheritability of gene expression traits on average.We further identified 235 and 298 fine-mapped STRs through the Bayesian fine-mapping approach in the F6 and F7 pigs,respectively,which were significantly enriched in intron,ATAC peak,compartment A and H3K4me3 regions.We identified 20 fine-mapped STRs located in 100 kb windows upstream and downstream of published complex trait-associated SNPs,which colocalized with epigenetic markers such as H3K27ac and ATAC peaks.These included eSTR of the CLPB,PGLS,PSMD6 and DHDH genes,which are linked with genome-wide association study(GWAS)SNPs for blood-related traits,leg conformation,growth-related traits,and meat quality traits,respectively.Conclusions:This study provides insights into the effects of STRs on gene expression traits.The identified eSTRs are valuable resources for prioritizing causal STRs for complex traits in pigs. 展开更多
关键词 Cis-eQTL CO-LOCALIZATION Gene expression LIVER Pig heterogeneous population short tandem repeats
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The study of engraft evidence in allogeneic bone marrow transplantation by 9 short tandem repeats loci
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《中国输血杂志》 CAS CSCD 2001年第S1期376-,共1页
关键词 BONE The study of engraft evidence in allogeneic bone marrow transplantation by 9 short tandem repeats loci
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STRs-PCR分型技术在法医学上的应用 被引量:6
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作者 范晶 许杨 熊勇华 《生物技术通报》 CAS CSCD 2003年第2期29-32,共4页
短串联重复序列 (STRs)是广泛存在于人类基因组的一类具有长度多态性的DNA序列 ,属高信息基因座。概述了STRs PCR的法医学应用特点以及它们在亲子鉴定、个体识别等领域的法医学应用及其理论基础、现状和前景。
关键词 strs-PCR分型技术 法医学 应用 亲子鉴定 个体识别 短串联重复序列 人类基因组 ’微卫星
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西藏藏族15个STRs位点多态性及其与其他民族群体的遗传关系 被引量:3
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作者 康龙丽 章晓风 +1 位作者 刘凯 赵健民 《中南大学学报(医学版)》 CAS CSCD 北大核心 2008年第9期800-808,共9页
目的:研究西藏藏族人群15个短串联重复序列(short tandem repeats,STRs)位点(D8S1179,D21S11,D7S820,CSF1PO,D3S1358,TH01,D13S317,D16S539,D2S1338,D19S433,vWA,TPOX,D18S51,D5S818,FGA)的多态性分布及群体遗传学和法医学应用价... 目的:研究西藏藏族人群15个短串联重复序列(short tandem repeats,STRs)位点(D8S1179,D21S11,D7S820,CSF1PO,D3S1358,TH01,D13S317,D16S539,D2S1338,D19S433,vWA,TPOX,D18S51,D5S818,FGA)的多态性分布及群体遗传学和法医学应用价值。并分析它们与西藏其他民族及其他亚洲人群间的遗传学关系。方法:采用ABI3100遗传分析仪检测STRs基因多态性,用ARLEQUIN 3.1软件计算等位基因频率和各种多态性参数。并将其结果与文献报道的其他亚洲人群的STRs结果进行比对,DISPAN软件计算遗传距离(DA)、基因分化系数(Gst)和杂合度(Ht),MEGA4.0软件绘制进化树,SPSS14.0进行多维量表法(MDS)分析。结果:藏族群体中共检出132种等位基因,频率分布0.0050-0.5990;杂合度、个体识别力、多态性信息量等群体遗传学指标分析显示,15个STRs位点具有中度或高度多态性,中国藏族群体具有较独立的遗传结构。结论:所选择的15个STRs位点具有较高的个体识别力和多态性信息量,可用于群体遗传学和法医学研究。 展开更多
关键词 短串联重复序列 群体遗传 进化树 多态性 藏族
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皖南地区汉族人群21号染色体上2个STRs位点的多态性分析 被引量:2
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作者 李慧 窦本芝 +1 位作者 李铁臣 孙青 《皖南医学院学报》 CAS 2010年第3期169-172,共4页
目的:研究21号染色体上2个短串联重复序列位点(D21S11、D21S1409)在安徽皖南地区汉族人群中的遗传多态性,评价它们在唐氏综合征产前诊断中的应用价值。方法:采用聚合酶链反应、聚丙烯酰胺凝胶电泳和银染技术,对皖南地区汉族无亲缘关系... 目的:研究21号染色体上2个短串联重复序列位点(D21S11、D21S1409)在安徽皖南地区汉族人群中的遗传多态性,评价它们在唐氏综合征产前诊断中的应用价值。方法:采用聚合酶链反应、聚丙烯酰胺凝胶电泳和银染技术,对皖南地区汉族无亲缘关系的90名个体的样本进行检测。结果:D21S11和D21S1409位点均由多个等位片段构成,片段大小分别在202~260bp和173~233p之间。两个位点的观察杂合度分别为0.4778和0.6222。结论:D21S1409有较高的杂合度,可作为唐氏综合征产前诊断的遗传标志。 展开更多
关键词 唐氏综合征 短串联重复序列 遗传多态性 产前诊断
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1例胎儿唐筛18三体高风险孕妇产前羊水细胞X染色体STRs相关位点基因拷贝数、细胞核型分析
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作者 龙若庭 陈淑霞 +3 位作者 林萃 罗华玉 李恋湘 李淑娜 《山东医药》 CAS 2021年第18期15-19,共5页
目的对1例胎儿唐氏筛查结果为18三体高风险孕妇的产前羊水细胞X染色体短串联重复序列(STRs)相关位点基因拷贝数、细胞染色体核型进行分析,明确胎儿的遗传学诊断。方法采集1例唐氏筛查结果为18三体高风险的孕妇羊水细胞,采用荧光定量聚... 目的对1例胎儿唐氏筛查结果为18三体高风险孕妇的产前羊水细胞X染色体短串联重复序列(STRs)相关位点基因拷贝数、细胞染色体核型进行分析,明确胎儿的遗传学诊断。方法采集1例唐氏筛查结果为18三体高风险的孕妇羊水细胞,采用荧光定量聚合酶链反应(达瑞生物、瑞典Devyser紧凑型V3非整倍体检测试剂盒和中德美联AGCU19X-STRs多重荧光扩增试剂盒)扩增羊水细胞DNA,检测X染色体上STRs相关位点,测算基因拷贝数,并分析其羊水细胞染色体核型。结果该孕妇产前羊水细胞X染色体Xq13区基因存在2个拷贝,其他区域基因仅有1个拷贝。X染色体上DXS10159、DXS10164、DXS10162、DXS10079、DXS10074等5个位点上有不平衡双峰,而其余14个XSTRs位点均为单峰。羊水细胞染色体核型为46,X,+ma[r33]/45,X[67]。结论唐筛18三体高风险孕妇的羊水细胞丢失部分X染色体,羊水细胞染色体核型为46,X,+ma[r33]/45,X[67]嵌合体核型。 展开更多
关键词 短串联重复序列 染色体核型 非整倍体 嵌合体 18三体综合征 羊水细胞
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Genetic Polymorphism of 38 Y-chromosome Short Tandem Repeats in Beijing Han Population from China
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作者 Yan Liu Chengtao Jiang +4 位作者 Dong Zhao Jinpei Zhang Libin Wu Di Lu Li Yuan 《Journal of Forensic Science and Medicine》 2023年第4期340-346,共7页
Objective:To investigate 38 Y-chromosome short tandem repeat(Y-STR)genetic polymorphisms in Beijing Han and analyze the genetic distance with neighboring or linguistically similar populations.Materials and Methods:In ... Objective:To investigate 38 Y-chromosome short tandem repeat(Y-STR)genetic polymorphisms in Beijing Han and analyze the genetic distance with neighboring or linguistically similar populations.Materials and Methods:In the study,we selected 531 unrelated male individuals of Beijing Han,and the results were statistically analyzed by testing with GSTAR™41Y reagents.Results:The allele peak heights were balanced among the Y loci,the amplified fragment ranged from 100 to 500 bps.A total of 531 haplotypes were detected in 531 samples.Eight null genotypes were observed on locus DYS448.One and three double alleles were observed on single-copy locus DYS576 and DYS19,respectively.DYS385 a/b,DYF387S1 a/b,and DYS527 a/b were more common in double copies,but 3,13,and 11 triple alleles were detected,respectively.The gene diversity values of Y-STRs except DYS391,DYS438,and DYS645 were>0.5.Twenty-seven Y-STRs of Beijing Han population were selected for genetic distance comparison with 17 populations including Changchun Han,with Rst values ranging from 0.0002 to 0.1703.Conclusion:The 38 Y-STRs in this study have strong male lineage identification ability and have great potential for individual identification,kinship identification,Y-STR database construction,and genetic relationship research. 展开更多
关键词 Genetic distance genetic polymorphism Han nationality in Beijing short tandem repeats Y-CHROMOSOME
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Genetic and structural characterization of 20 autosomal short tandem repeats in the Chinese Qinghai Han population and its genetic relationships and interpopulation differentiations with other reference populations
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作者 Zhanhai Wang Bin Lu +3 位作者 Xiaoye Jin Jiangwei Yan Haotian Meng Bofeng Zhu 《Forensic Sciences Research》 2018年第2期145-152,共8页
China is a multinational country composed of 56 ethnic groups of which the Han Chinese accounts for 91.60%.Qinghai Province is located in the northeastern part of the Qinghai-Tibet Plateau,has an area of 72.12 km2,and... China is a multinational country composed of 56 ethnic groups of which the Han Chinese accounts for 91.60%.Qinghai Province is located in the northeastern part of the Qinghai-Tibet Plateau,has an area of 72.12 km2,and is the fourth largest province in China.In the present study,we investigated the genetic polymorphisms of 20 short tandem repeat (STR) loci in a Qinghai Han population,as well as its genetic relationships with other populations.A total of 273 alleles were identified in 2 000 individuals at 20 loci,and the allelic frequency ranged from 0.0002 to 0.5327.The 20 STR loci showed a relatively high polymorphic rate in the studied group.Observed and expected heterozygosities ranged 0.613 0-0.907 5 and 0.614 8-0.920 0,respectively.The combined power of discrimination,and the probability of exclusion in duo and trio cases were 0.999 999 999 999 999 999 999 999 34,0.9999960 and 0.9999999965,respectively.Analyses of interpopulation differentiation revealed that the most significant differences were found between the Qinghai Han and Malaysian,while no significant differences were found between the Qinghai Han and Han people from Shaanxi and Jiangsu.The results of principal component analysis,multidimensional scaling analysis and phylogenetic reconstructions also suggested the close relationships between the Qinghai Han and other two Han populations.The present results,therefore,indicated that these 20 STR loci could be used for paternity testing and individual identification in forensic applications,and may also provide information for the studies of genetic relationships between Qinghai Han and other groups. 展开更多
关键词 Genetic polymorphisms forensic genetics phylogenetic reconstruction short tandem repeat
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Identification of Half‑Sisters from Different Mothers by Autosomal and X Chromosomal Short Tandem Repeats:A Case Study
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作者 Jinpei Zhang Shicheng Hao +1 位作者 Yan Liu Li Yuan 《Journal of Forensic Science and Medicine》 2021年第2期66-69,共4页
Complex kinship identification such as half‑sibling identification is a difficult task in forensic biology Here we represented an approach in dealing with half‑sisters from different mothers,with the combination of au... Complex kinship identification such as half‑sibling identification is a difficult task in forensic biology Here we represented an approach in dealing with half‑sisters from different mothers,with the combination of autosomal and X chromosomal short‑tandem repeats(STRs)data.X chromosomal STRs can offer additional information,especially in some cases where autosomal STRs alone may not provide enough information for an accurate opinion.In this case,half‑sister or unrelated relationship between two women(S_(1)and S_(2))with different mothers were distinguished.23 autosomal and 31 X chromosomal STRs of S_(1),S_(2),S_(1)’s mother(M1),S_(2)’s mother(M2)and S_(1)’s grandmother(G1)were profiled with three different commercial kits.As to X‑chromosome STRs,likelihood ratios(LRs)were calculated by FamLinkX with consideration of linkage,linkage disequilibrium,and mutations.When only the profiles of the two individuals(S_(1)and S_(2))were available,LRs between S_(1)and S_(2)were 1.1110×10^(2)based on 23 autosomal STRs and 3.2257 om107 based on 31 X chromosomal STRs.When the maternal genotypes were taken into consideration,LRs increased to 2.5297×10^(3)and 3.0563×10^(18).Therefore,both the DNA profiles of each mothers and X chromosomal STRs are important in dealing with the identification of half‑sisters from different mothers. 展开更多
关键词 Forensic case half‑sisters likelihood ratio short tandem repeat X chromosomal short tandem repeat
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A NORTHWEST DATABASE MODEL OF SHORT TANDEM REPEAT LOCI IN FORENSIC MEDICINE 被引量:1
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作者 王振原 朱波峰 +6 位作者 刘雅诚 严江伟 霍振义 金天博 李涛 樊拴良 方杰 《Journal of Pharmaceutical Analysis》 SCIE CAS 2003年第1期93-96,110,共5页
Objective To establish the northwest database of short tandem repeat(STR) loci in forensic medicine. Methods Bloodstains or whole blood samples were collected from the unrelated prisoners in Xi'an city. Genetic ... Objective To establish the northwest database of short tandem repeat(STR) loci in forensic medicine. Methods Bloodstains or whole blood samples were collected from the unrelated prisoners in Xi'an city. Genetic distribution for 13 STR loci and amelogenin locus were determined in prisons based on GeneScan. One primer for each locus was labeled with the fluorescent by 5 FAM, JOE, or NED. The forensic database were generated by using multiple amplification, GeneScan, genotype, and genetic distribution analysis. Results 113 alleles and 302 genotypes were observed, with the corresponding frequency between 0.0050-0.5250 and 0.0100-0.4100. The mean H was 0.7667. The accumulative DP was 0.9999999,. The accumulative EPP was 0.9999999. The scope of PIC was 0.6036- 0.8562 . PM was less than 10 -11 . The observed and expected genotype frequencies were evaluated using χ 2 test and all were in accordance with Hardy Weinberg equilibrium ( P > 0.05 ). Conclusion STR loci is an ideal genetic marker with powerful polymorphism and stable heredity. It can be used for individual identification and paternity in forensic medicine. The forensic DNA database model can be established successfully. 展开更多
关键词 short tandem repeat(STR) DNA database GENESCAN polymerase chain reaction GENOTYPE
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Carrier Detection and Presymptomatic Identification of Wilson Disease in Chinese by Non-Isotopic Linkage Analysis with Four Short Tandem Repeat Polymorphisms 被引量:1
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作者 吴志英 王柠 +1 位作者 慕容慎行 阮旭中 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 1999年第1期51-53,66,共4页
Summary: Wilson disease (WD) is an autosomal recessive disorder of copper metabolism. To establish an efficient, accurate and fast diagnostic method for carrier detection and presymptomatic identification of WD in Chi... Summary: Wilson disease (WD) is an autosomal recessive disorder of copper metabolism. To establish an efficient, accurate and fast diagnostic method for carrier detection and presymptomatic identification of WD in Chinese population, we studied haplotypes of short tandem repeat (STR) polymorphisms flanking the WD gene in 40 Chinese WD families. The results suggested that this genetic diagnosis system based on the four STR polymorphisms is of high value for the detection of potential carriers and WD homozygotes in families with at least one previously affected child. It is an efficient, accurate and fast diagnostic method that can be well suited for routine use in clinical laboratories. 展开更多
关键词 Wilson disease short tandem repeat gene diagnosis
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ALLELE DISTRIBUTION OF FIVE X-CHROMOSOME SHORT TANDEM REPEAT LOCI IN EWENKE POPULATION OF NORTH CHINA
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作者 Shan-zhi Gu Teng Chen Qing-bo Liu Bing Yu Sheng-bin Li 《Chinese Medical Sciences Journal》 CAS CSCD 2005年第4期237-241, ,共5页
Objective To study the allele genetic polymorphism of five short tandem repeat (STR) loci on X-chromosome in Ewenke population of north China and to provide basic data for forensic identification. Methods Genomic D... Objective To study the allele genetic polymorphism of five short tandem repeat (STR) loci on X-chromosome in Ewenke population of north China and to provide basic data for forensic identification. Methods Genomic DNA was extracted from EDTA-whole blood of Ewenke population by Chelex-100. The DNA samples were amplified by PCR and were analyzed by polyacrylamide gel electrophoresis and silver staining. The sequence length variations of DXS6799, DXS8378, DXS101, HPRTB, and DXS6789 loci on X-chromosome in 98 unrelated Ewenke individuals were investigated. Results All five loci analyzed showed high polymorphism and genetic stability. The data of the five X-chromosome STR loci in Ewenke ethnic group of China was in accordance with Hardy-Weinberg equilibrium by Chi-square test. Conchusion Allele polymorphism of five X-chromosome STR loci can be used as a genetic marker for forensic identification and population genetic research. 展开更多
关键词 allele distribution X-CHROMOSOME short tandem repeat Ewenke population
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Application of Short Tandem Repeat in Prenatal Diagnosis for Phenmylketonuria during the First Trimester
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作者 赵晓岚 叶国玲 +3 位作者 楚雍烈 刘琪 蔡晓宁 李明丽 《Journal of Nanjing Medical University》 2003年第2期58-61,共4页
Objective : To find a simple and rapid way far the prenatal diagnosis of phenyUce-tonuria (PKU) during the first trimester in order to prevent inborn PKU patients as early as possible. Methods :DNA was extracted respe... Objective : To find a simple and rapid way far the prenatal diagnosis of phenyUce-tonuria (PKU) during the first trimester in order to prevent inborn PKU patients as early as possible. Methods :DNA was extracted respectively from the Mood sampleps of 9 families' members and chori-onic tissues of 9 embryoes by cliorionic vittus sampling (CVS). The independent short tandem repeat (STR) alleles of members in 9 families with classic form of PKU were analyzed and prenatal diagnosis were conducted using polymerase chain reaction (PCR) together with denaturing gradient gel elec-trophoresis(DGGE)and silver dyeing. Results-.We identified 1 embryo with PKU, 2 normal individuals and 5 carriers among 9 subjects. Conclusion: Prenatal diagnosis for PKU by STR is available in the first trimester. This procedure was promising and would be widely used in Chinese population. 展开更多
关键词 prenatal diagnosis PHENYLKETONURIA short tandem repeat first trimester
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Distribution of six short tandem repeat (STR) loci in Yugu ethnic group in Gansu province of China
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《中国输血杂志》 CAS CSCD 2001年第S1期363-,共1页
关键词 STR Distribution of six short tandem repeat
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Study on the application of short tandem repeat (SIR) complex amplication technique in difficult cases of paternity test
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《中国输血杂志》 CAS CSCD 2001年第S1期368-,共1页
关键词 SIR complex amplication technique in difficult cases of paternity test Study on the application of short tandem repeat
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Forensic investigation of 23 autosomal STRs and application in Han and Mongolia ethnic groups 被引量:5
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作者 Xiang Sheng Yali Wang +5 位作者 Jiashuo Zhang Liqin Chen Yuan Lin Zhenmin Zhao Chengtao Li Suhua Zhang 《Forensic Sciences Research》 2018年第2期138-144,共7页
A forensic validation study of the Early Access HuaxiaTM Platinum Polymerase Chain Reaction (PCR) kit was completed to document the performance capabilities and limitations.The genotyping of DNA samples was consistent... A forensic validation study of the Early Access HuaxiaTM Platinum Polymerase Chain Reaction (PCR) kit was completed to document the performance capabilities and limitations.The genotyping of DNA samples was consistent across a large range of template DNA concentrations,with complete profiles obtained at 0.125 ng;however,no more than 2 mm× 1.2 mm punches of samples would be recommended for direct amplification.The size precision and accuracy test revealed the genotyping ability;while consistent results were obtained when comparing the kit with other commercially available systems.In addition,the whole PCR amplification can finish within approximately 45 min,making the system suitable for fastdetection.However,only partial profiles may be obtained with challenging samples,including DNA stored on Foam-Tipped Applicators (FTA) cards or some case samples.For the forensic application in ethnic groups,a total of 282 and 229 alleles were obtained in Han and Mongolia,respectively.Since the 23 short tandem repeats were independent from each other,the cumulative power of exclusion in duos was 0.999999157188 and the cumulative power of exclusion in trios was 0.999999999859 in the Han group while the cumulative power of exclusion in duos (CPEduo) was 0.999 998 848 26 and cumulative power of exclusion in trios (CPEtrio) was 0.999 999 999 79 in the Mongolia group.And good internal consistency was found between the two investigated groups and the Sichuan Han,Hui,Tibetan and Uygur according to available reference data. 展开更多
关键词 Forensic genetics short tandem repeats(strs) Early Access HuaxiaTM Platinum PCR kit
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Genetic study on nines hort tandem repeat (STR) loci among Han population in southern China and their application of parentage tests
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《中国输血杂志》 CAS CSCD 2001年第S1期364-,共1页
关键词 STR Genetic study on nines hort tandem repeat loci among Han population in southern China and their application of parentage tests
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深圳汉族人群42个常染色体短串联重复序列基因座的遗传多态性
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作者 钟艳平 伍立桃 +5 位作者 李桢 周丹 全湛柔 梁爽 邓志辉 张胤鸣 《中山大学学报(医学科学版)》 CAS CSCD 北大核心 2024年第5期739-744,共6页
【目的】调查深圳汉族人群42个常染色体短串联重复序列(STR)基因座(含41个非CODIS系统STR基因座)等位基因的遗传多态性,研究其在法医鉴定中的应用价值。【方法】采用AGCU21+1和阅微MR23荧光扩增试剂盒对深圳汉族人群435个无关个体STR基... 【目的】调查深圳汉族人群42个常染色体短串联重复序列(STR)基因座(含41个非CODIS系统STR基因座)等位基因的遗传多态性,研究其在法医鉴定中的应用价值。【方法】采用AGCU21+1和阅微MR23荧光扩增试剂盒对深圳汉族人群435个无关个体STR基因座进行序列多态性分析。通过Modified-Powerstates和arlequin v3.5软件统计等位基因频率、法医遗传学参数并进行Hardy-Weinberg平衡检验。【结果】深圳汉族人群435个无关个体共检出418个等位基因,均符合Hardy-Weinberg平衡定律(P>0.05/42),频率分布在0.0011~0.5529之间。D1S1656和D21S1270基因座多态性最高,均检出16个等位基因;D4S2408基因座检出的等位基因最少;个体识别能力(DP)为0.7988(D1S1627)~0.9686(D7S3048),多态性信息含量(PIC)为0.5680(D1S1627)~0.8598(D7S3048),杂合度(H)为0.6276(D1S1627)~0.8782(D20S470)。【结论】42个常染色体STR基因座的等位基因在深圳地区汉族群体遗传多态性较好,具有较高的个体识别能力,在个体识别和亲权鉴定尤其是单亲或出现基因突变的情况下具有较高的应用价值;所得的数据亦为STR群体遗传学提供基础数据。 展开更多
关键词 法医遗传学 遗传多态性 常染色体 短串联重复序列 深圳 汉族
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